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Plasmid#134808PurposeIPTG inducible expression of Y-TAGA-Evo1 qtRNADepositorInsertY-TAGA-Evo1 qtRNA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
B5-4
Plasmid#111483PurposeTrp-tRNA synthetaseDepositorInsertTrp-tRNA synthetase
UseTags5xHisExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-GST-CD(Cbx7)
Plasmid#82525PurposeExpresses GST-CD (Cbx7) fusion proteins in E. coli. Cbx7 chromodomain only; amino acids 1–62.DepositorInsertChromobox Homolog 7 (CBX7 Human)
UseTagsGSTExpressionBacterialMutationCD(Cbx7) ; amino acids 1–62PromoterAvailable sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEERM6 Km
Plasmid#64027PurposePlasmid for integration into sqs site of Synechocystis 6803DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPnrsBAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBA1018
Plasmid#189587PurposeT4wtGT7 deletion HR vector 250bp HR Arms (T4 phage editing)DepositorInsertT4wtGT7 Deletion Locus 250bp Homology Arms
UseSynthetic BiologyTagsExpressionBacterialMutationEncodes for a full deletion of T4 from 52.1 (part…PromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMPMA3dPlac-PsopB-GFPLVA
Plasmid#23341DepositorInsertGFP(LVA)
UseTagsGFP(LVA)ExpressionBacterialMutationPromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBAD-iGECInano
Plasmid#186189PurposeExpression of small FRET-based near-infrared genetically-encoded calcium indicator in bacteriaDepositorInsertiGECInano
UseTagsExpressionBacterialMutationPromoterAraCAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBXC3H
Plasmid#47068PurposeFX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site and 10x His tagDepositorTypeEmpty backboneUseTags3C protease site (PreScission site) and decaHis (…ExpressionBacterialMutationPromoteraraBADAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S129G
Plasmid#36049DepositorInsertalpha-synuclein S129G (SNCA Human)
UseTagsExpressionBacterialMutationS129GPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
4QQ6
Plasmid#61946PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSMN1:JMC047-F11:C221089 (SMN1 Human)
UseTags6xHis and MHHHHHHSSGRENLYFQGExpressionBacterialMutationSee commentsPromoterT7Available sinceMarch 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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KG#371
Plasmid#110882PurposeExpresses the lysosome marker CTNS-1-mCherry cDNA in a subset of ventral cord cholinergic motor neuronsDepositorInsertsunc-129 promoter
CTNS-1a-mCherry cDNA
unc-54 3' control region with 1 intron just upstream and 1 intron in control region
UseTagsintron-mCherryExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOGG009
Plasmid#113983PurposeVector construction module. Gentamicin resistance module for construction of Golden Gate cloning vectorsDepositorInsertGentamicin
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTKEI-tLOV
Plasmid#100574PurposeExpression plasmid for the thermostable anaerobic fluorescent protein tLOVDepositorInserttLOV
UseTags6xHisExpressionBacterialMutationPromoterpTrcAvailable sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK100
Plasmid#73264PurposeProduces Bacillus subtilis phosphoribosylformylglycinamidine synthase small subunit (BsPurS)DepositorInsertphosphoribosylformylglycinamidine synthase subunit PurS
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBXNH3LCA_TM287/288
Plasmid#132701Purposeexpresses TM287/288 in E.coli, to use as positive control in ELISADepositorUseTagsAvi and HisExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp3a_Ub1
Plasmid#156467PurposeBacterial expression of Sars-CoV2 Nsp3a_Ub1 protein with His-tag and GST-tagDepositorInsertNsp3a_Ub1 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGR-ECK120033127
Plasmid#46011DepositorInsertECK120033127 Terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pL-rpb1-N488D
Plasmid#10988DepositorInsertrpb1-N488D (RPO21 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMutation 1462 A to G in the RPB1 ORF resulting in…PromoterAvailable sinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pETM33_CEP55_EABR
Plasmid#178469PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertCEP55_EABR (CEP55 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBXNH3A
Plasmid#47075PurposeFX cloning E.coli expression vector with araBAD promoter and N-terminal 10x His, 3C protease cleavage site and Avi-tagDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), Avi-tag, and…ExpressionBacterialMutationPromoteraraBADAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYTK079
Plasmid#65186PurposeEncodes HygromycinR as a Type 6 part to be used in the Dueber YTK systemDepositorInsertHygromycinR
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLacIq-Wasabi
Plasmid#86112PurposemWasabi reporter gene driven by LacIq promoterDepositorInsertmWasabi
UseTags6xHisExpressionBacterialMutationPromoterpLacIqAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Skp2 WT
Plasmid#81117Purposebacterial expression plasmid of Skp2DepositorInsertSKP2 (SKP2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCW-LIC-N8C_Fab1a
Plasmid#203687PurposeExpresses N8C_Fab1aDepositorInsertN8C_Fab1a
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAtipiTrc
Plasmid#53275PurposeContains an Arabidopsis thaliana idi1 cDNA fused to a strong Trc promoter. Produces an active isopentenyl diphosphate isomerase (IDI) in E. coli.DepositorInsertipi1 (IPP2 Mustard Weed)
UseTagsExpressionBacterialMutationPromoterTrcAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GP-2-DNMT3A PWWP
Plasmid#59696PurposeContains histone modification interacting domain (DNMT3A PWWP)DepositorInsertDNMT3A PWWP (Dnmt3a Mouse)
UseTagsExpressionBacterialMutationPromotertac promoterAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO5-SgHottip-GFP-CRISPRa
Plasmid#134990PurposedCas9-mediated activation of HOTTIP in mammalian cellsDepositorInsertHOTTIP (HOTTIP Human)
UseLentiviralTagsGFPExpressionBacterial and MammalianMutationPromoterU6Available sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a_His-TEV-EiCsm6
Plasmid#172487PurposeBacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.DepositorInsertEnterococcus italicus Csm6 (codon optimized)
UseTagsHis6ExpressionBacterialMutationPromoterT7 promoter and lac operatorAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMP1071
Plasmid#119245Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUWL201PW-creDE
Plasmid#112241PurposeStreptomyces/E.coli shuttle expression vector encoding creD and creEDepositorInsertscreD
creE
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B16-TEF(203)
Plasmid#44377DepositorInsertUAS1B16-TEF(203) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B16-TEF(203)Available sinceApril 16, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEB1-comCerulean3
Plasmid#104017PurposePlasmid encoding comCerulean3DepositorInsertcomCerulean3
UseLow copyTagsExpressionBacterialMutationSame protein sequence as mCerulean3 (codon optimi…PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pMALp2x-SCO5461
Plasmid#42507DepositorInsertguanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))
UseTagsmaltose-binding proteinExpressionBacterialMutationPromotertacAvailable sinceApril 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
BASIC_8_RBS34- mCherry -B0015
Plasmid#68142Purposestorage vector for BASIC part: RBS-RFP(ORF)DepositorInsertRBS34-mcherry-B0015
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mRFP1
Plasmid#104001PurposePlasmid encoding mRFP1DepositorInsertmRFP1
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (R2A, K5E, N6D, T21S,…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pV2A-T
Plasmid#153022PurposeSingle-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoterDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-soNadV
Plasmid#83363PurposeExpresses nadV from Shewanella oneidensis in Escherichia coliDepositorInsertnadV
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBTBXh-4
Plasmid#26080DepositorTypeEmpty backboneUseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-cytoBirA-2a-mCherry
Plasmid#79885PurposeUbiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseTags3x HAExpressionBacterialMutationPromoterUbiquitinAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T2 SHP2 E76A
Plasmid#8324DepositorInsertSHP2 (PTPN11 Human)
UseTagsGSTExpressionBacterialMutationE76A (activated mutant)PromoterAvailable sinceJune 17, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P1-Vinculin tail
Plasmid#107154Purposebacterial expressionDepositorInsertVinculin (VCL Human)
UseTagsGSTExpressionBacterialMutationPromoterPtacAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS mPrx1 (CT#640)
Plasmid#13961DepositorInsertPrx1 promoter (Prrx1 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSCBe
Plasmid#110065PurposeTrc1O promoter express gene in Synechocystis 6803DepositorTypeEmpty backboneUseTags3' end His tag and 5' end FLAG tagExpressionBacterialMutationPromoterTrc1OAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP TEV co-transformation cloning vector (13K-C)
Plasmid#48317DepositorTypeEmpty backboneUseTagsHis6 and MBPExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1 deltains1
Plasmid#78461PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
UseTagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; delta 2149-2170; Q2513R (please see…PromoterT7Available sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdestDD03
Plasmid#26414DepositorInsertattR4-ccdB-attR2 cassette
UseTagsExpressionBacterial and WormMutationPromoterAvailable sinceApril 23, 2012AvailabilityAcademic Institutions and Nonprofits only