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32,156 results

Showing: 8841 - 8880 of 32156 results

  2. 6CCR (GID4)

    Plasmid
    #119960
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    GID4 (C17orf39 Human)
    Tags
    6xHIS, thrombin,SUMO and TEV cleavage
    Expression
    Bacterial
    Mutation
    contains amino acids 116-300
    Promoter
    T7
    Available Since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119960-plasmid-map-sequence-id-232678

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  3. DNMT3A

    Plasmid
    #25328
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DNMT3A_11 (DNMT3A Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 17, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25328-plasmid-map-sequence-id-356911

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  6. 4A3.PBAD-repL(WT).J23115-gfp

    Plasmid
    #197872
    Purpose
    Arabinose induced expression of wildtype repL gene, which triggers DNA replication in cis and in trans, weak level of constitutive GFP expression
    Depositor
    Baojun Wang
    Article
    Huan et al Front Microbiol. 2023 Feb 9;14:1095671.
    Inserts
    part kilA/repL
    gfp
    Expression
    Bacterial
    Mutation
    contains only the 24 bp of 3'-end of kilA ge…
    Promoter
    Bba_J23115 and PBAD
    Available Since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197872-plasmid-map-sequence-id-395257

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  11. htpG2-GFP

    Plasmid
    #109388
    Purpose
    GFP cassette under the control of the htpG2 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.
    Depositor
    Tom Ellis
    Article
    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463
    Insert
    phtpG2-GFP
    Expression
    Bacterial
    Available Since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    109388-plasmid-map-sequence-id-210731

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  12. pOPINE-3C-YFP

    Plasmid
    #214028
    Purpose
    Expression of fluorescent fusion proteins
    Depositor
    Ray Owens
    Insert
    YFP
    Tags
    Hexahistidine
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    214028-plasmid-map-sequence-id-422812

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  13. UBC80

    Plasmid
    #25267
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubc80.229.304 (TSG101 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. pIYN3

    Plasmid
    #197099
    Purpose
    To express the variant of M. jannaschii TyrRS specific for halogenated tyrosines and Nap3 amber suppressor tRNA and allow UAG codon to be translated into these derivatives.
    Depositor
    Kensaku Sakamoto
    Article
    Mukai et al Biochem Biophys Res Commun. 2011 Aug 12;
    Insert
    TyrRS variant, Nap3 tRNA
    Expression
    Bacterial
    Mutation
    Ala70, Thr158, Ser159, Arg286 (TyrRS)
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    197099-plasmid-map-sequence-id-387837

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  23. pET28a-B4E

    Plasmid
    #162067
    Purpose
    For expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4E
    Depositor
    Karen Polizzi
    Article
    Moya-Ramirez et al Nucleic Acids Res. 2020 Dec 16;48(22):e1
    Insert
    ampR-eIF4E fusion protein
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    eIF4E incorporating the K119A mutation, which is …
    Promoter
    T7
    Available Since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    162067-plasmid-map-sequence-id-325536

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  30. pKD072

    Plasmid
    #136472
    Purpose
    SSB-C-term-GFP fusion expression plasmid
    Depositor
    James Keck
    Article
    Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60
    Insert
    SSB-C-term-GFP (ssb E. coli)
    Tags
    superfolder GFP
    Expression
    Bacterial
    Mutation
    superfolder GFP attached to C-terminus at Phe 178
    Promoter
    T7 promoter
    Available Since
    April 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    136472-plasmid-map-sequence-id-267962

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  32. HDAC7

    Plasmid
    #25478
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HDAC7 (HDAC7 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. pHis-TEV-Nsp15

    Plasmid
    #187656
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Jernigan et al Structure. 2022 Dec 30:S0969-2126(22)004
    Insert
    Nsp15 (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7lac
    Available Since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    187656-plasmid-map-sequence-id-388768

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  38. JMJD1CA-c089

    Plasmid
    #79644
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    JMJD1C (JMJD1C Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    79644-plasmid-map-sequence-id-150204

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Showing: 8841 - 8880 of 32156 results