31,059 results
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Plasmid#68449PurposeTOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible geneDepositorInserttTR-KRAB-2A-rtTA3-2A-Luciferase
UseLuciferase; GmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-G6
Plasmid#73437PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.DepositorInsertRepressor G6 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable SinceMarch 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAmeR-F1
Plasmid#74675PurposeAmeR-F1 gateDepositorInsertPTac-RBSF1-AmeR-PAmeR-YFP
ExpressionBacterialAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-3a_mb2m
Plasmid#211368PurposeExpresses murine b2mDepositorInsertb2m (B2m Mouse)
ExpressionBacterialAvailable SinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQCasTns(Vch)-entry(BsaI)
Plasmid#190273PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(BsaI)
ExpressionBacterialMutationnoAvailable SinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTG131
Plasmid#198640PurposeThermosensitive pSC101-based plasmid expressing a sgRNA under the control of the J23119 promoter and Cas9 under the control of the DAPG-inducible PhlF promoterDepositorInsertCas9
ExpressionBacterialPromoterpPhlFAvailable SinceJune 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
C163
Plasmid#141127PurposeExpresses sfCFP under lac promoterDepositorInsertsfCFP
TagsssrA degradation tagExpressionBacterialPromoterengineered lac promoterAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
C164
Plasmid#141128PurposeExpresses sfYFP under lac promoterDepositorInsertsfYFP
TagsssrA degradation tagExpressionBacterialPromoterengineered lac promoterAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
As2
Plasmid#123160PurposeArsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, ParsR-ABS84, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLIParaF.Ec-250n
Plasmid#18855DepositorInsertarabinose FRET sensor
TagsCFP and VenusExpressionBacterialAvailable SinceNov. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mTurquoise2
Plasmid#103974PurposePlasmid encoding mTurquoise2DepositorInsertmTurquoise2
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F64L, Y66W, …PromoterproCAvailable SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbA4c-RFP
Plasmid#35299DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterProEAvailable SinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-TEV-IFIT3
Plasmid#53559Purposebacterial expression of IFIT3DepositorAvailable SinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2 I427V
Plasmid#81044Purposemammalian expression of LOV2 I427VDepositorInsertLOV I427V
TagsmCherryExpressionBacterial, Insect, and Mamm…MutationI427VAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pACYC.GFP1Amb
Plasmid#102792PurposeExpresses GFP with a premature stop codonDepositorInsertGreen Fluorescent Protein
ExpressionBacterialMutationY39StopAvailable SinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET23a RanGAP
Plasmid#13387DepositorInsertRanGAP (RANGAP1 Human)
ExpressionBacterialAvailable SinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
Human HMG-CoA lyase (HL)
Plasmid#52433Purposebacterial expression of human HMG-CoA lyase (HL)DepositorAvailable SinceApril 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a_DmKHC[1-421]-SNAP-6xHis
Plasmid#196975PurposeBacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tagDepositorAvailable SinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-GFP-mHP1alpha
Plasmid#181912PurposeBacterial protein expression vector for GFP-tagged HP1alphaDepositorAvailable SinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_WDR5_WD
Plasmid#178491PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTYB2-eIF1
Plasmid#37217DepositorInserteIF1
ExpressionBacterialPromoterT7Available SinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-D91G
Plasmid#47646PurposepAC-EsaR with a a272g mutation in esaRDepositorInsertesaR-D91G
UseSynthetic BiologyExpressionBacterialMutationChanged Aspartic acid 91 to GlycinePromoterPlacAvailable SinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETM6-C4-mCherry
Plasmid#66530PurposeExpresses mCherry under the control of Mutant T7 Promoter C4DepositorInsertmCherry
UseSynthetic BiologyExpressionBacterialMutationCodon Optimized for E. coliPromoterMutant T7 Promoter - C4Available SinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
POLArIS_NbVim-cp10GFP
Plasmid#171020PurposeExpresses NbVim-cp10GFP#2 in mammalian cellsDepositorInsertanti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)
Tagscp10GFPExpressionBacterial and MammalianPromoterCMV promoterAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAM12
Plasmid#86471PurposeExpresses p12 and p9-mCitrineDepositorInsertp12, p9-mCitrine
UseSynthetic BiologyTagsmCitrineExpressionBacterialPromoterpBADAvailable SinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCas9-beta-mmr
Plasmid#169241PurposeCas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutationsDepositorInsertscas9
beta
mutL-E36K
UseCRISPR and Synthetic BiologyExpressionBacterialMutationChanged glutamic acid 36 to lysineAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
T7-U4
Plasmid#111336Purposefor in vitro snRNA transcriptionDepositorInsertsnR14
ExpressionBacterialAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
UIMLx2
Plasmid#174888PurposeUIMLx2 ubiquitin probe for K48 ubiquitylated proteinsDepositorInsertMet4 UIML
TagsAvi-6xHis-Smt3ExpressionBacterialAvailable SinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
METT10D
Plasmid#25485PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
SBC009876
Plasmid#226283PurposeExpresses PhCFAT and ObEGS1 from trc promoter. Biosynthesis of phenylpropenes from (hydroxy)cinnamyl alcohols.DepositorInsertsConiferyl alcohol acyltransferase
Eugenol synthase 1
ExpressionBacterialAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-amidase
Plasmid#128135PurposeThe cell-free adapted backbone, pBEAST, expressing the amidase enzyme gene (benzamid to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertamidase
UseSynthetic BiologyExpressionBacterialPromoterJ23101Available SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAJM.944
Plasmid#108523PurposePCin-YFP Reporter. OHC14 sensor for use in Marionette strain.DepositorInsertPCin-YFP
ExpressionBacterialAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK_proK1_eGFP
Plasmid#59853PurposeConstitutively expresses eGFP with proK1 promoterDepositorInsertEnhanced Green Fluorescent Protein
Tags6x-HisExpressionBacterialMutationS2R,S65G,S72APromoterproK1Available SinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTn7xTS-mPlum
Plasmid#117392PurposeTn7 tagging vector pTn7xTS with mPlum expression scaffoldDepositorInsertmPlum
UseSynthetic BiologyExpressionBacterialPromoterPtacAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-EZH2-T487A mutant
Plasmid#28061DepositorAvailable SinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-Num1-PH
Plasmid#71369Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertNum1-PH
TagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa2563-2692PromotertacAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-mUbp43-His
Plasmid#12458DepositorAvailable SinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pQE-60NA-mScarlet-2I
Plasmid#160463PurposeEncodes a variant of mScarlet-I that displays reduced cytotoxicity in bacteriaDepositorInsertmScarlet-2I
ExpressionBacterialPromoterT5Available SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046833
Plasmid#145649PurposeBacterial Expression plasmid for SARS-CoV-2 nsp9DepositorInsertSARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX Abl-SH2
Plasmid#46402DepositorInsertAbl (ABL1 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 120-225PromoterTacAvailable SinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only