32,163 results
-
Plasmid#111486PurposePro-tRNA synthetaseDepositorInsertPro-tRNA synthetase
Tags6xHisExpressionBacterialAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD-SYNZIP3
Plasmid#80659PurposeEncodes the synthetic coiled coil peptide SYNZIP3DepositorInsertSYNZIP3
Tags6xHisExpressionBacterialAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b-Casp8 delta DED C360A
Plasmid#11828DepositorInsertCaspase 8 minus Death Effecter Domain catalytic mutant (CASP8 Human)
Tags6xHisExpressionBacterialMutationMinus Death Defecter Domain (aa 1-216), C360AAvailable SinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-Sumo-IntNtr-eSrt2A
Plasmid#126015PurposeBacterial expression of IntNtr-eSrt2A fusion protein (with N-terminal His6-Sumo)DepositorInsertHis6-Sumo-IntNtr-eSrt2A
Tags6xHis-SUMOExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBTRCk-pVhb-maACR
Plasmid#215651PurposepBBR1 Origin, KanR, with maACR gene under Vhb promoterDepositorInsertacyl-CoA reductase
TagsMaltose binding protein and His tagExpressionBacterialPromoterVhbAvailable SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-pVhb-vfFadBA
Plasmid#215650PurposepACYC Origin, CmR, with vfFadBA gene under Vhb promoterDepositorInsertthiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
ExpressionBacterialPromoterVhbAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJK_ToeRepG2_N19_trigger
Plasmid#132747PurposeT7 RNAP-driven expression of second-generation toehold repressor index 19 trigger RNADepositorInsertToeRepG2_N19_trigger
ExpressionBacterialAvailable SinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCold TDP43 G294A
Plasmid#27452DepositorInsertTDP-43 (TARDBP Human)
TagsFactor Xa and His6ExpressionBacterialMutationGlycine 294 mutated to Alanine (G294A)Available SinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBJG1 T7-8xHis-OGT 5N5A
Plasmid#154280PurposeBacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
Tags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationN322A, N356A, N390A, N424A, N458A mutations; bact…Available SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
ACOT11A
Plasmid#42424PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDZ207 (loxP KAN loxP 24xPP7SL MDN1)
Plasmid#35191DepositorInsertloxP KAN loxP 24xPP7SL MDN1
UseCre/LoxExpressionBacterialAvailable SinceFeb. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYTK035
Plasmid#65142PurposeEncodes I-SceI (ORF) as a Type 3 part to be used in the Dueber YTK systemDepositorInsertI-SceI (ORF)
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
pT7-7 asyn S87E/S129E
Plasmid#36064DepositorAvailable SinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRGM1
Plasmid#48789PurposeHA-I-SceI expression plasmid, episomalDepositorInsertI-SceI
TagsHA tagExpressionBacterialPromoterMOPAvailable SinceJan. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
MetNI (Lm)
Plasmid#78316PurposeMethionine ABC TransporterDepositorInsertsMetI
MetN
Tags10x Histidine Tag and 6x Histidine TagExpressionBacterialPromoterT7 PromoterAvailable SinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R472A_H477A
Plasmid#83483PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).DepositorInsertLbu_C2c2_R472A_H477A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR472A, H477AAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLOXp-GUS
Plasmid#154059PurposeLevel 0.5 Golden Gate vector, loxP-GUS-nosT-loxPDepositorInsertbeta-glucoronidase (E. coli)
UseSynthetic BiologyExpressionBacterialPromoterN/AAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTHSSd_18
Plasmid#59953PurposeQuad split (at 61, 179, 601-SZ)DepositorInsertFour-fragment T7* RNA polymerase
ExpressionBacterialMutationN/APromoterPTacAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1R1AtR2B2
Plasmid#160880PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana rubisco chaperone proteins raf2, and bsd2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
ExpressionBacterialPromoterrbcS-S1: T7, chaperones: T7Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-lambdaRED_NOC
Plasmid#127032PurposeBasic Part NOC- Complete ORF; LambdaRED / _RED; Policistronic "NOC" part with coding regions for the three lambda red proteins (the first; gam; doesn't have RBS; but bet and exo genes have RBS).DepositorInsertPart ?RED_NOC
UseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKM263-EcPPK
Plasmid#38333DepositorInsertE. coli polyphosphate kinase (ppk Escherichia coli)
Tags6xHIS, GST, and TEVExpressionBacterialPromoterT7Available SinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBLO5.01
Plasmid#121418PurposepUC19_BsaI-dCas9-BsaIDepositorInsertdcas9
ExpressionBacterialPromoterlacAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJBL7026
Plasmid#128811PurposeFluoride riboswitch-regulated 3-way junction dimeric broccoliDepositorInsertFluoride riboswitch-regulated 3-way junction dimeric broccoli
UseSynthetic BiologyExpressionBacterialAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP E17TAG/Q157TAG
Plasmid#68299PurposeE17/Q157 TAG GFP reporterDepositorInsertGFP E17TAG/Q157TAG
TagsHisExpressionBacterialAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQCascade-IS186
Plasmid#140627PurposeExpresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS186. The crRNA-IS186 targets IS186 loci in Escherchia coli.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS186
UseCRISPR; TransposonExpressionBacterialAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
AD-003
Plasmid#25502PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pETM33_Orf9b
Plasmid#156485PurposeBacterial expression of Sars-CoV2 Orf9b protein with His-tag and GST-tagDepositorInsertOrf9b
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Intersectin I SH3 A domain (human)
Plasmid#47413DepositorInsertIntersectin SH3 domain A (ITSN1 Human)
TagsGSTExpressionBacterialMutationonly SH3 domain APromotertacAvailable SinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLII12Pglu-600u
Plasmid#13563DepositorInsertFLIPglu F16A Y12S-ECFP-D13R
TagsCitrineExpressionBacterialMutationF16A ECFP insertion in binding proteinAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
attB Neo* attP
Plasmid#138412PurposeattB and attP-flanked pPgk-pEM7-Neo*-bpA positive selection cassetteDepositorInsertNeo*
UseMouse Targeting; Att/phic31ExpressionBacterial and MammalianAvailable SinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJB066
Plasmid#98913PurposeExpress FtsZ tagged with pAmCherry1 in E coliDepositorInsertFtsZ
TagspAmCherry1ExpressionBacterialPromoterpBADAvailable SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS hBAF170
Plasmid#17875DepositorInsertBAF170 (SMARCC2 Human)
ExpressionBacterialAvailable SinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
Endophilin Full
Plasmid#47408DepositorInsertEndophilin
TagsGSTExpressionBacterialPromotertacAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
J-HARS-4
Plasmid#166529PurposescFv of a human scaffold targeting Histidyl-tRNA synthetase. Antigen coverage aa 1-509 of 509, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
L-SARS-7
Plasmid#166527PurposescFv of a human scaffold targeting Seryl-tRNA synthetase. Antigen coverage aa 1-514 of 514, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX Hck-SH2
Plasmid#46445DepositorInsertHck (HCK Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 134-254PromoterTacAvailable SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET23b-Casp9 C287A-His
Plasmid#11830DepositorAvailable SinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTU2S-a (colE1 origin)
Plasmid#74091PurposeSecondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 2 TU's with secondary module and colE1 origin
UseSynthetic BiologyExpressionBacterialPromoterNoneAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB088i
Plasmid#79216PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-TnCAD-F3
ExpressionBacterialPromoterPlacZ-opt (OR1); Ppro1Available SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only