30,514 results

1. pBad33_ISDge10_reRNA

   Plasmid

   #205340

   Purpose

   Bacterial expression of ISDge10 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   ISDge10 TnpB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pnsgRNA

   Plasmid

   #172717

   Purpose

   Delivery of guide RNA for prime editing

   Depositor

   Tilmann Weber

   Article

   Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

   Insert

   J23119-gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP

   Plasmid

   #198753

   Purpose

   Cre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activity

   Depositor

   Jacco van Rheenen

   Article

   Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:

   Insert

   EF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   See depositor comments below

   Promoter

   EF1a

   Available Since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEB2-E2-Crimson

   Plasmid

   #104010

   Purpose

   Plasmid encoding E2-Crimson

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   E2-Crimson
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to DsRed (R2D, S4T, K5E, E10P,…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWM_12x601_20bpLinker

   Plasmid

   #157787

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 20 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_20bp linker
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNitET-SacB-kan

   Plasmid

   #107692

   Purpose

   Expression of the phage Che9c RecET system for Recombineering in mycobacteria

   Depositor

   Kenan Murphy

   Article

   Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

   Inserts

   Che9 phage RecET
   PNit2-NitR
   SacRB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNO286-3

   Plasmid

   #107746

   Purpose

   Key genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)

   Depositor

   Jeffrey Tabor

   Article

   Ong et al Chembiochem. 2018 Feb 8. doi: 10.1002/cb

   Inserts

   CcaS
   Ho1
   PcyA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Deleted AA 223 and 512

   Promoter

   J23106 and J23108

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWM_12x601_45bpLinker

   Plasmid

   #157791

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_45bp linker
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. ERG_pet28a

   Plasmid

   #131642

   Purpose

   Bacterial expression of ETS gene ERG

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ERG (ERG Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21-pelB-LaG-16-4GS-LaG-2

    Plasmid

    #172764

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16-4GS-LaG-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBJ2

    Plasmid

    #167131

    Purpose

    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Kanamycin promoter

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWM_12x601_35bpLinker

    Plasmid

    #157790

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 35 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_35bp linker
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-SisTnpB1–ωRNA

    Plasmid

    #228365

    Purpose

    Expresses SisTnpB1 and RNP complex in E. coli Rosetta (DE3) cells

    Depositor

    Tao Liu

    Article

    Xu et al Commun Biol. 2024 Nov 20;7(1):1541. doi:

    Insert

    SisTnpB1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBbS5c-mCherry-AsLOV2*(543)

    Plasmid

    #202712

    Purpose

    Expresses an IPTG inducible mCherry fused to a blue light responsive degradation tag (LOVdeg)

    Depositor

    Mary Dunlop

    Article

    Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

    Insert

    mCherry-AsLOV2*(543)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC28-LIC-sfGFP-His

    Plasmid

    #165479

    Purpose

    pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

    Depositor

    Renato Bruni

    Article

    Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

    Type

    Empty backbone

    Use

    Tags

    TEV-GFP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAB167

    Plasmid

    #101672

    Purpose

    Opto-T7RNAP*(563), equal expr.

    Depositor

    Mustafa Khammash

    Article

    Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

    Insert

    Opto-T7RNAP*(563), equal expr.
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-MinE-His

    Plasmid

    #133623

    Purpose

    Expression vector for the Escherichia coli MinE with an C-terminal fusion of a His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Glock et al ACS Synth Biol. 2018 Dec 20. doi: 10.102

    Insert

    MinE-His (minE E. coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. YL-CashGem-BAT1-URA3-LINEAR

    Plasmid

    #174837

    Purpose

    pRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolytica

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    Codon optimized Cas9:hGem
    

    Use

    CRISPR and Synthetic Biology

    Tags

    hGeminin tag

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    GPDp

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX6P1-human LIC1 C-terminal half

    Plasmid

    #74599

    Purpose

    used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
    

    Use

    Tags

    N-terminal GST and Strep II

    Expression

    Bacterial

    Mutation

    amino acids 389-523 of LIC1

    Promoter

    T7

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTYB2-eIF1A

    Plasmid

    #37218

    Depositor

    Jon Lorsch

    Article

    Acker et al Methods Enzymol. 2007;430:111-45.

    Insert

    eIF1A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only