34,148 results
-
Plasmid#13422DepositorTypeEmpty backboneUseCre/LoxExpressionBacterialAvailable sinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by
-
pET His6 MBP TEV LIC cloning vector (1M)
Plasmid#29656DepositorTypeEmpty backboneTags6xHis, MBP, and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCas9-CR4
Plasmid#62655PurposeCas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetRDepositorInsertCas9 nuclease
ExpressionBacterialAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMJ806
Plasmid#39312DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialPromoterT7Available sinceSept. 7, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRISPomyces-2
Plasmid#61737PurposeStreptomyces expression of codon-optimized Cas9 and custom gRNADepositorPublicationInsertssSpCas9
gRNA cassette
UseCRISPRExpressionBacterialMutationBbsI-flanked lacZ cassette inserted in place of s…Promotergapdhp(EL) and rpsL(XC)-BbsIAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX6P1-mCherry-Nanobody
Plasmid#70696PurposeExpresses GST-tagged anti-mCherry Nanobody in E. coli cells.DepositorInsertmCherry-Nanobody
TagsGSTExpressionBacterialAvailable sinceMarch 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
tau/pET29b
Plasmid#16316DepositorInserttau (MAPT Human)
ExpressionBacterialAvailable sinceDec. 21, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKD4
Plasmid#45605DepositorInsertFRT-kan-FRT
ExpressionBacterialPromoterR6KAvailable sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDZ2087
Plasmid#92414PurposeExpresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coliDepositorInsertTEV protease
TagsMBP (self cleaving)ExpressionBacterialMutationL56V/S135G/S219VPromotertacAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOPINE GFP nanobody
Plasmid#49172PurposeBacterial expression of GFP nano bodyDepositorInsertGFP nanobody
TagsHHHHHHExpressionBacterial and MammalianPromoterT7Available sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT3TS-nCas9n
Plasmid#46757Purposeexpression of an optimized Cas9 for genome editing in zebrafishDepositorInsertCas9
UseCRISPRTagsSV40 NLSExpressionBacterialPromoterT3Available sinceAug. 6, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET His6 Sumo TEV LIC cloning vector (1S)
Plasmid#29659DepositorTypeEmpty backboneTags6xHis, SUMO, and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKDsgRNA-p15
Plasmid#62656PurposeArabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmidDepositorInsertexo, beta, gam, sgRNA
ExpressionBacterialAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TurboID-His6_pET21a
Plasmid#107177Purposeexpresses His6-tagged TurboID in bacteriaDepositorInsertTurboID (BirA mutant)
ExpressionBacterialMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Available sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAD-mTagBFP2
Plasmid#34632DepositorInsertmTagBFP2
ExpressionBacterialMutationI174A compared to mTagBFP (enhanced photostabilit…Available sinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
-
pSANG10-3F-BG4
Plasmid#55756PurposeExpresses BG4 scFv anti-G-quadruplex antibody in bacteriaDepositorInsertBG4
Tags6xHis, pelB leader, and tri-FLAGExpressionBacterialPromoterT7Available sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pwtCas9-bacteria
Plasmid#44250PurposeaTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdownDepositorInsertwild-type Cas9
UseCRISPRExpressionBacterialPromoterpLtetO-1Available sinceApril 11, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTriEx-RhoA FLARE.sc Biosensor WT
Plasmid#12150DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRhoA Biosensor (RHOA Human)
Tags6xHisExpressionBacterial, Insect, and Mamm…Available sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTriEx-mCherry-PA-Rac1
Plasmid#22027PurposeExpression of photoactivatable Rac1DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPA-Rac1 (RAC1 Human, Avena sativa (oat))
Tags6xHis and mCherryExpressionBacterial, Insect, and Mamm…MutationLOV(Leu404-Leu546), Rac1 starts at Ile4 and conta…PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by