32,370 results
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Plasmid#180454PurposeE. coli expression of soluble MHC proteinDepositorInsertH-2D(b)
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_45bpLinker
Plasmid#157791PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_45bp linker
ExpressionBacterialAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pExp-sfGFP
Plasmid#112573PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8xHis, Strep II, and sfGFPExpressionBacterialAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDJSVT87
Plasmid#109302PurposeExpress periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and His6 tagDepositorTypeEmpty backboneTagsOmpA 1-27, 6HisExpressionBacterialPromoterT7Available SinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-ALFA-TwinStrep
Plasmid#240248PurposeArabinose-inducible plasmid with ALFA and Twin-Strep tagsDepositorTypeEmpty backboneTags10x HIS, ALFA, Thrombin, and Twin-StrepExpressionBacterialPromoteraraBADAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SET
Plasmid#234708Purposeexpresses His-tagged human SET in E coliDepositorAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
CBP TAZ1(340-439)
Plasmid#173760Purposebacterial expression of mouse CBP TAZ1 domainDepositorAvailable SinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
HisGb1-STAT1(710-750) + CBP TAZ2 (1764-1855)
Plasmid#99342PurposeBacterial expression of mouse CBP TAZ2 domain by coexpression with HisGB1 tagged STAT1 TADDepositorAvailable SinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pA-Opto
Plasmid#253255PurposeOpto-CheY expressed using the proA promoter and T7 RBS variant GAAGGAGgTDepositorInsertOpto-CheY
ExpressionBacterialMutationT7 RBS variant GAAGGAGgTAvailable SinceApril 6, 2026AvailabilityAcademic Institutions and Nonprofits only -
p5g-Opto_N449S
Plasmid#253285PurposeOpto-CheY N449S expressed using the pro5 promoter and T7 RBS variant GAAGGAGgTDepositorInsertOpto-CheY with N449S mutation
TagsT7 RBS variant GAAGGAGgTExpressionBacterialAvailable SinceApril 6, 2026AvailabilityAcademic Institutions and Nonprofits only -
p30T crRNA55/120
Plasmid#250980PurposeExpresses two Cas13a guide RNAs targeting orf55 and orf120 in phiKZDepositorInsertDual CRISPR array expressing two Cas13a crRNAs to target orf55 and orf120 in phiKZ
ExpressionBacterialAvailable SinceMarch 31, 2026AvailabilityAcademic Institutions and Nonprofits only -
pUCminiTn7-Mariner-flag
Plasmid#250982PurposepUC mini-Tn7 inserting Mariner transposase-flag into genomeDepositorInsertMariner transposase with a FLAG tag
ExpressionBacterialAvailable SinceMarch 31, 2026AvailabilityAcademic Institutions and Nonprofits only -
pHERD30T-TnV1acrVIA1
Plasmid#250983PurposepHERD30T vector providing the transposon donor with acrVIA1DepositorInsertTransposon donor V1 encoding acrVIA1
ExpressionBacterialAvailable SinceMarch 31, 2026AvailabilityAcademic Institutions and Nonprofits only -
pHERD20T-V3TnAcrVIA1
Plasmid#250984PurposepHERD20T vector providing the transposon donor with acrVIA1DepositorInsertTransposon donor V3 encoding acrVIA1
ExpressionBacterialAvailable SinceMarch 31, 2026AvailabilityAcademic Institutions and Nonprofits only -
pA_higher-Opto_N449S
Plasmid#253258PurposeOpto-CheY N449S expressed using the proA promoter and wild type T7 RBSDepositorInsertOpto-CheY with N449S mutation
Tagswild type T7 RBSExpressionBacterialAvailable SinceMarch 19, 2026AvailabilityAcademic Institutions and Nonprofits only -
pA-Opto_N449S
Plasmid#253256PurposeOpto-CheY N449S expressed using the proA promoter and T7 RBS variant GAAGGAGgTDepositorInsertOpto-CheY with N449S mutation
TagsT7 RBS variant GAAGGAGgTExpressionBacterialAvailable SinceMarch 19, 2026AvailabilityAcademic Institutions and Nonprofits only -
pA-Opto_V416T
Plasmid#253257PurposeOpto-CheY V416T expressed using the proA promoter and T7 RBS variant GAAGGAGgTDepositorInsertOpto-CheY with V416T mutation
ExpressionBacterialMutationT7 RBS variant GAAGGAGgTAvailable SinceMarch 19, 2026AvailabilityAcademic Institutions and Nonprofits only -
pET29b_SpyTag003-L-VHH21-His
Plasmid#233333PurposeTo express nanobody VHH21 with N terminal SpyTagDepositorInsertSpyTag003-L-VHH21-His
ExpressionBacterialPromoterT7Available SinceOct. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-a(+)-6xHis-mBlueberry(-9)
Plasmid#219416PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertmBlueberry2(-9)
TagsMGHHHHHGGExpressionBacterialMutationK137E, K199E, R234EPromoterT7Available SinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
in-frame ampicillin PKAKTR backbone
Plasmid#177197PurposePKAKTR mutagenesis template with in-frame ampicillin siteDepositorTypeEmpty backboneExpressionBacterialAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only