30,888 results

1. pET28a-cMSP1D1

   Plasmid

   #199768

   Purpose

   Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSP1D1
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   external His-Tags flanking the C and N termini

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKL2299A

   Plasmid

   #222105

   Purpose

   A ternary vir helper plasmid that carries Agrobacterium tumefaciens virulence genes for enhanced plant genetic transformation.

   Depositor

   Kan Wang

   Article

   Aliu et al Front Plant Sci. 2024 Jul 23;15:1429353.

   Insert

   virA
   

   Expression

   Bacterial and Plant

   Promoter

   virA promoter

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF1-Pls3-sfGFP

   Plasmid

   #205750

   Purpose

   Expresses sfGFP under IPTG inducible promoter Pls3 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Medium/High strength.

   Depositor

   Jerome Bonnet

   Article

   Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95

   Type

   Empty backbone

   Use

   Shuttle vector gram+ gram-

   Expression

   Bacterial

   Promoter

   Pls3 (Phyperspank mutant, IPTG inducible)

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. p11d-tRPA(123)

   Plasmid

   #102613

   Purpose

   Expression of human RPA in E. coli

   Depositor

   Marc Wold

   Article

   Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

   Inserts

   RPA1 (RPA1 Human)
   RPA2 (RPA2 Human)
   RPA3 (RPA3 Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF1-Pls1-sfGFP

   Plasmid

   #205748

   Purpose

   Expresses sfGFP under IPTG inducible promoter Pls1 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Low strength.

   Depositor

   Jerome Bonnet

   Article

   Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95

   Type

   Empty backbone

   Use

   Shuttle vector gram+ gram-

   Expression

   Bacterial

   Promoter

   Pls1 (Phyperspank mutant, IPTG inducible)

   Available Since

   Aug. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-cMSP26

   Plasmid

   #199769

   Purpose

   Expresses split-intein fused MSP26 for circularization during expression (internal His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSP26
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   internal His-Tag

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-cMSPdH4-6

   Plasmid

   #199766

   Purpose

   Expresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSPdH46
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   internal His-Tag

   Available Since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBad-EBFP2

   Plasmid

   #14891

   Purpose

   Bacterial expression of EBFP2 blue fluorescent protein

   Depositor

   Robert Campbell

   Article

   Ai et al Biochemistry. 2007 May 22;46(20):5904-10

   Has Service

   Cloning Grade DNA

   Insert

   EBFP2
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   The fluorescent protein was inserted between XhoI…

   Available Since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. L4440_BioBrick-sgRNA

   Plasmid

   #177783

   Purpose

   Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL004

    Plasmid

    #215336

    Purpose

    amyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank-Hy promoter)

    Depositor

    Gene-Wei Li

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    hyper-pSpank

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTU2-AmyE-Pveg-eGFP

    Plasmid

    #112792

    Purpose

    Expresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformation

    Depositor

    Chris French

    Article

    Wicke et al bioRxiv 185108

    Insert

    Pveg-eGFP
    

    Expression

    Bacterial

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEVOL-pBpF

    Plasmid

    #31190

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.

    Depositor

    Peter Schultz

    Article

    Chin et al Proc Natl Acad Sci U S A. 2002 Aug 20;99

    Insert

    M.j. p-benzoylphenylalanine RS (2 copies +tRNA)
    

    Expression

    Bacterial

    Mutation

    Y32G, E107P, D158T, I159S

    Available Since

    Dec. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEvol-pAcFRS.2.t1

    Plasmid

    #73544

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAcFRS.2.t1
    pAcFRS.2.t1
    

    Expression

    Bacterial

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pF4Ag NanoLuc (ATG-42)

    Plasmid

    #137777

    Purpose

    Expresses NanoLuc in multi promoter expression vector (CMV andT7 promoters)

    Depositor

    Lance Encell

    Article

    Hall et al ACS Chem Biol. 2012 Nov 16;7(11):1848-57

    Insert

    NanoLuc (Nluc)
    

    Expression

    Bacterial and Mammalian

    Mutation

    NanoLuc contains 16 amino acid changes compared t…

    Promoter

    T7 and CMV

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPR-PsppA-mCherry

    Plasmid

    #225428

    Purpose

    Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherry

    Depositor

    Geir Mathiesen

    Article

    Wiull et al Microbiol Spectr. 2025 Feb 4;13(2):e0202

    Insert

    mCherry, Cas9, SgRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PsppA upstream of mCherry and Cas9, P3 upstream o…

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28c-F30-Broccoli

    Plasmid

    #66788

    Purpose

    Expresses Broccoli in the F30 scaffold in bacterial cells

    Depositor

    Samie Jaffrey

    Article

    Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

    Insert

    Broccoli in the F30 scaffold
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPROEX Aqua

    Plasmid

    #42889

    Depositor

    Fabienne Merola

    Article

    Erard et al Mol Biosyst. 2013 Feb 2;9(2):258-67. doi

    Insert

    Aquamarine
    

    Tags

    6xHis and TEV cleavage

    Expression

    Bacterial

    Mutation

    T65S and H148G

    Available Since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. 10xHis-3C-BRD4(42-459) BD1-BD2

    Plasmid

    #233886

    Purpose

    pRSF-DUET1 for protein overexpression of human BRD4

    Depositor

    Alessio Ciulli

    Article

    Hsia et al Nature. 2024 Mar;627(8002):204-211. doi:

    Insert

    BRD4 (BRD4 Human)
    

    Tags

    10x his 3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBJ2

    Plasmid

    #167131

    Purpose

    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    Kanamycin promoter

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRE118

    Plasmid

    #43830

    Depositor

    Dieter Schifferli

    Article

    Edwards et al Gene. 1998 Jan 30;207(2):149-57.

    Type

    Empty backbone

    Use

    Bacterial allelic exchange vector with sacb1

    Expression

    Bacterial

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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