29,712 results

1. pET28_6xHis-PARP1-CATdeltaHD

   Plasmid

   #174811

   Purpose

   Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-CATdeltaHD (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Residues 678-787 replaced by eight-residue linker…

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pYTK072

   Plasmid

   #65179

   Purpose

   Encodes ConRE as a Type 5 part to be used in the Dueber YTK system

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Insert

   ConRE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-mAmetrine-CNA35

   Plasmid

   #61604

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with mAmetrine

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   mAmetrine and CNA35
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPM2213

   Plasmid

   #167553

   Purpose

   Encodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12

   Depositor

   Renato Morona

   Article

   Macpherson et al Mol Microbiol. 1991 Jun;5(6):1491-9. doi

   Insert

   rfb region of Shigella flexneri serotype 2a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET51b_HCAII

   Plasmid

   #187110

   Purpose

   Expression of HCAII in bacteria

   Depositor

   Kai Johnsson

   Article

   Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

   Insert

   HCAII
   

   Use

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28-SaCas9

   Plasmid

   #178901

   Purpose

   SaCas9 (Cas9 from Staphylococcus aureus)

   Depositor

   Hendrik Milting

   Article

   Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

   Insert

   SaCas9
   

   Use

   Tags

   His6-Tag, bipartite nuclear localization signal f…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pQTEV-MAT2B

   Plasmid

   #34795

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

   Insert

   methionine adenosyltransferase II, beta (MAT2B Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEx-4T-1-YTHDF1

   Plasmid

   #70087

   Purpose

   Encode YTHDF1 with N-terminal GST tag for E. coli expression

   Depositor

   Chuan He

   Article

   Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

   Insert

   YTH N(6)-methyladenosine RNA binding protein 1 (YTHDF1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDule- Mj Acd A9

   Plasmid

   #197651

   Purpose

   E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair

   Depositor

   Ryan Mehl

   Article

   Sungwienwong et al Org Biomol Chem. 2017 May 3;15(17):3603-

   Inserts

   Mj Acd A9 synthetase
   Methanocaldococcus jannaschii tRNA
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32A, L65D, F108L, Q109E, D158S, L162T

   Promoter

   GlnS and lpp

   Available since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET32-MCM2/7

    Plasmid

    #116949

    Purpose

    Expresses human MCM2 and human MCM7

    Depositor

    James Chong

    Article

    Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

    Inserts

    Human MCM7 (MCM7 Human)
    Human MCM2 (MCM2 Human)
    

    Use

    Tags

    Thioredoxin, His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFW1000

    Plasmid

    #224210

    Purpose

    E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGP-RSET-iGABASnFR2-6xHis

    Plasmid

    #218885

    Purpose

    Bacterial expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    T7

    Available since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pVoPo-mNG

    Plasmid

    #187843

    Purpose

    Constitutive mNeonGreen-expression vector for Fusobacterium nucleatum

    Depositor

    Jörg Vogel

    Article

    Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28_mouse-Caspase-1_enzymatic-domain

    Plasmid

    #183385

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1 (AA130-404) (Casp1 Mouse)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSC5GGv1

    Plasmid

    #194645

    Purpose

    pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 1)

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCFT4.2

    Plasmid

    #208807

    Purpose

    Expresses the fluorescent protein phiLOV2.1

    Depositor

    François-Xavier Campbell-Valois

    Article

    Tchagang et al Front Microbiol. 2023 Oct 20;14:1245755.

    Insert

    phiLOV2.1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RNAPII_CTD27-52

    Plasmid

    #98678

    Purpose

    Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

    Depositor

    Nicolas Fawzi

    Article

    Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

    Insert

    RNA polymerase II C-terminal domain (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDG04439

    Plasmid

    #216365

    Purpose

    xhNup133-Nb2t (+2Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup133-Nb2t (+2Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFLxR3

    Plasmid

    #167518

    Purpose

    Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Tetracycline Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    LuxR-mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLuxB

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZ8-Ptac

    Plasmid

    #74064

    Purpose

    IPTG inducible version of pZ8-1 plasmid, to which lacIq was added, KanR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ptac

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRK172-α-syn-TEV-GFP

    Plasmid

    #166671

    Purpose

    Bacterial expression of alpha synuclein coupled to GFP via a TEV site for uptake studies

    Depositor

    Iris Lindberg

    Article

    Jarvela et al PLoS One. 2021 Jan 26;16(1):e0241161. do

    Insert

    mouse synuclein (from V. Lee) (Snca Mouse)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    added a TEV cleavage site sequence between synucl…

    Promoter

    CMV

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGEX-human p53-(1-393)

    Plasmid

    #24860

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBEST-p15a-pBAD-UTR1-TetR-T500

    Plasmid

    #45787

    Depositor

    Richard Murray

    Insert

    Tetracycline repressor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHV738

    Plasmid

    #117476

    Purpose

    Expression of NMT1

    Depositor

    Richard Kahn

    Article

    Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

    Insert

    NMT1 (NMT1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAH01Sp::Cas9

    Plasmid

    #128160

    Purpose

    Ptet-controlled expression of S. pyogenes Cas9

    Depositor

    Calvin Henard

    Article

    Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

    Insert

    Sp Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TS181_pETNKI_his3C_pA-Dam

    Plasmid

    #200721

    Purpose

    producing pA-DamID methylation protein in bacteria

    Depositor

    Bas van Steensel

    Article

    van Schaik et al Methods Mol Biol. 2022;2458:215-229. doi

    Insert

    His3C-pA-Dam
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized

    Promoter

    Available since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pACYCT2

    Plasmid

    #45799

    Depositor

    Luc Ponchon

    Article

    Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CD Ugi4

    Plasmid

    #177196

    Purpose

    Greengate entry vector pGGC000 modified to contain bladderwort insulator 4

    Depositor

    Wayne Parrott

    Article

    Park et al bioRxiv 2021.11.04.467280

    Insert

    Utricularia gibba insulator 4
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. KE-005

    Plasmid

    #133440

    Purpose

    Human elF4A1 residues 1-406, N-term His tag, thrombin cleavage, in pET21 vector backbone.

    Depositor

    Gerhard Wagner

    Insert

    EIF4A1 (EIF4A1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. tracrRNA(scr)-CJ8421_04975 mRNA

    Plasmid

    #199718

    Purpose

    Expresses the Campylobacter mRNA CJ8421_04975 and tracrRNA with scrambled anti-repeat sequencesequence

    Depositor

    Chase Beisel

    Article

    Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

    Insert

    CJ8421_04975 mRNA and scrambled tracrRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCR8-attL3-pegRNA-attL2

    Plasmid

    #213052

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL3 and attL2

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    35S-CmYCLV-AtU6

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid

    #223065

    Purpose

    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    

    Use

    CRISPR

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Bacterial

    Mutation

    Three regions of SpCas9 encode type IIS restricti…

    Promoter

    Dual T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRExT2A-CT-PmSc

    Plasmid

    #232549

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-PAC-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    mScarlet
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pTKW106alp7A

    Plasmid

    #69360

    Purpose

    Stabilized lacZ expression vector containing alp7A and hok/sok

    Depositor

    Jeff Hasty

    Article

    Danino et al Sci Transl Med. 2015 May 27;7(289):289ra

    Inserts

    lacZ
    alp7A
    hok
    placIq-lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTac and placIq

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBLADE_ONLY_C

    Plasmid

    #168050

    Purpose

    Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

    Depositor

    Barbara Di Ventura

    Article

    Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

    Insert

    Blue light-inducible AraC dimers in Escherichia coli
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101**

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGEx-4T-1-YTHDF3

    Plasmid

    #70088

    Purpose

    Encode YTHDF3 isoform a with N-terminal GST tag for E. coli expression

    Depositor

    Chuan He

    Article

    Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

    Insert

    YTH N(6)-methyladenosine RNA binding protein 3 (YTHDF3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pCDF-His-mTET1CD

    Plasmid

    #81053

    Purpose

    bacterial expression of the catalytic domain of murine TET1

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TET1 (Tet1 Mouse)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    catalytic domain amino acid 1367-2057

    Promoter

    T7

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET-16b-10His-TEV-MCES_VACCA_D1_D12

    Plasmid

    #203671

    Purpose

    The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage

    Depositor

    Stephen Cusack

    Article

    Kyrieleis et al Structure. 2014 Mar 4;22(3):452-65. doi:

    Insert

    MCES_VACCA_D1_D12
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDEST-periHisMBP

    Plasmid

    #11086

    Depositor

    David Waugh

    Article

    Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

    Type

    Empty backbone

    Use

    Tags

    6xHis, MBP, and MBP signal peptide

    Expression

    Bacterial

    Mutation

    Promoter

    Tac (lactose/IPTG inducible)

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pST-KT

    Plasmid

    #44561

    Purpose

    for ectopic inducible expression of mycobacterial proteins

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    UV15 promoter

    Available since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET28a-6xHis-MBP-TEV site-TadA8e

    Plasmid

    #208319

    Purpose

    Overxpression MBP-TadA8e

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8e
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutations in TadA are described in the paper

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pSC101-pT7-T3RNAP

    Plasmid

    #140872

    Purpose

    T7 promoter driven synthesis of T3 RNA polymerase

    Depositor

    Neal Devaraj

    Article

    Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

    Insert

    T3 RNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET21a-C2-SNAP

    Plasmid

    #208769

    Purpose

    To express C2-SNAP in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2-SNAP (Mfge8 Mouse)
    

    Use

    Tags

    SNAP-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pExchange_tdk_BT1311p-tetR-1

    Plasmid

    #90325

    Purpose

    Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 4861701 and 4861702

    Depositor

    Andrew Goodman

    Article

    Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

    Insert

    TetR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pINTO_7/8

    Plasmid

    #206431

    Purpose

    A vector backbone (pCC1BAC-based) containing a single SapI recognition site. When digested, it exposes overhangs that allow integration of the vector into the P. tricornutum chloroplast genome.

    Depositor

    Bogumil Karas

    Article

    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pBTR1

    Plasmid

    #22468

    Depositor

    Gary Pielak

    Article

    Olteanu et al Biochem Biophys Res Commun. 2003 Dec 19.

    Insert

    cytochrome c (CYCS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pNCS mStrawberry

    Plasmid

    #91768

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Strawberry (mStrawberry)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Insert

    monomeric Strawberry
    

    Use

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. Clomeleon

    Plasmid

    #203723

    Purpose

    A genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentration

    Depositor

    George Augustine

    Article

    Kuner et al Neuron. 2000 Sep;27(3):447-59. doi: 10.1

    Insert

    cyan fluorescent protein linked yellow florescent protein
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Cyan Fluorescent Protein, Topaz Fluorescent Prote…

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pMSR0

    Plasmid

    #161984

    Purpose

    Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile ribotype 027 and other ribotypes. Uses inducible expression of CD2517.1 toxin as a counter-selection marker

    Depositor

    Olga Soutourina

    Article

    Peltier et al Commun Biol. 2020 Nov 27;3(1):718. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pET15b-ACPs (from S. pneumonia)

    Plasmid

    #63687

    Purpose

    Inducible expression of Acyl Carrier Protein Synthase (ACPs) from S. pneumoniae

    Depositor

    Michael Johnson

    Insert

    acpS
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only