32,069 results

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pETDuet-1-chmI

Plasmid

#221341

Purpose

Express ChmI in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmI

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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Human Parkin (1-465)

Plasmid

#110758

Purpose

Bacterial expression of human Parkin

Depositor

David Komander

Article

Wauer et al EMBO J. 2013 Jul 31;32(15):2099-112. doi

Insert

PRKN (PRKN Human)

Tags

N-His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pH6HTC_PRMT1-Halo

Plasmid

#175334

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

PRMT1 (PRMT1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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p6XHis_NLS-SaCas9

Plasmid

#101086

Purpose

Express SaCas9 in bacteria with a 6xHis tag for purification

Depositor

Rick Tarleton

Article

Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

Insert

CRISPR-associated protein Cas9/Csn1

Use

CRISPR

Tags

TrxA-6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pdCas9-J23111

Plasmid

#113149

Purpose

Plasmid constitutively expressing dCas9 protein

Depositor

Chong Zhang

Article

Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

Insert

dCas9

Expression

Bacterial

Mutation

D10A & H840A

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMRE163

Plasmid

#118511

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Expression

Bacterial

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pWSK29

Plasmid

#172972

Purpose

Low copy number cloning vector

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtS1R1R2B2

Plasmid

#160882

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcS-S1, rbcX, raf1, raf2, bsd2

Expression

Bacterial

Promoter

rbcS-S1: T7, chaperones: T7

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP1354

Plasmid

#119275

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pET24a-ncOGT-FL

Plasmid

#190821

Purpose

For expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

O-GlcNAc Transferase (OGT Human)

Tags

6x His

Expression

Bacterial

Mutation

Codon optimized for expression in E.coli

Promoter

T7

Available Since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pET21a-GlnRS-His

Plasmid

#124108

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glutaminyl-tRNA ligase (glnS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)_NADP-Snifit

Plasmid

#113913

Purpose

Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NADP-Snifit

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAB061i

Plasmid

#79226

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-HA4; PlacIq lacI

Expression

Bacterial

Promoter

Ppro1; PlacIq

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a FKBP12-V5

Plasmid

#194117

Purpose

Expresses FKBP12 protein with V5 tag

Depositor

Jun Liu

Insert

FKBP1A (FKBP1A Human)

Expression

Bacterial

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_FYB-1/2

Plasmid

#91459

Purpose

Protein expression and purification of human SH3 domain construct FYB-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

FYB-1/2 (FYB1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_FYB-2/2

Plasmid

#91300

Purpose

Protein expression and purification of human SH3 domain construct FYB-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

FYB-2/2 (FYB1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pQE30-His-PheRS

Plasmid

#124116

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pJMK001

Plasmid

#33780

Depositor

Adam Cohen

Article

Kralj et al Science. 2011 Jul 15;333(6040):345-8.

Insert

Proteorhodopsin Optical Proton Sensor

Expression

Bacterial

Mutation

D97N

Promoter

AraBAD

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pRK172 asyn(1-103)

Plasmid

#240597

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

Ni et al J Mol Biol. 2019 Sep 6;431(19):3913-3919

Insert

aSyn (SNCA Human)

Expression

Bacterial

Mutation

aa 1-103 only

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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14x PP7 with loxP-kanMX-loxP

Plasmid

#189939

Purpose

Integration of 14xPP7 loops in Saccharomyces cerevisiae. Selection by KanMX (flanked by LoxP sites for marker removal)

Depositor

Tineke Lenstra

Article

Donovan et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

Insert

14x PP7 with loxP-kanMX-loxP

Tags

kanMX

Expression

Bacterial

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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NP-11-64-1

Plasmid

#234359

Purpose

Expressing rubisco and prk in Δrpi

Depositor

David Savage

Article

Prywes et al Nature. 2025 Jan 22. doi: 10.1038/s41586

Insert

R.rubrum rubisco

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptac

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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ETV7_pet28a

Plasmid

#131656

Purpose

Bacterial expression of ETS gene ETV7

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV7 (ETV7 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pRibo-APEX2m

Plasmid

#176842

Purpose

Expression of APEX2 from a codon-optimized gene (multi-copy episomal plasmid)

Depositor

Jessica Seeliger

Article

Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

Insert

pRibo-APEX2m

Expression

Bacterial

Promoter

pRibo, theophilline riboswitch inducible hsp60 pr…

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PYCR1

Plasmid

#38842

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PYCR1 (PYCR1 Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
AP1

Plasmid

#179678

Purpose

Accessory plasmids

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[SD8] gIII

Expression

Bacterial

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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PT7lacO-EF-Ts-His6

Plasmid

#224371

Purpose

Express EF-Ts protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al ACS Synth Biol. 2025 May 16;14(5):1496-1

Insert

EF-Ts (tsf E. coli)

Tags

His6

Expression

Bacterial

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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PT7lacO-EF-G-His8

Plasmid

#224369

Purpose

Express EF-G protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al ACS Synth Biol. 2025 May 16;14(5):1496-1

Insert

EF-G (fusA E. coli)

Tags

His8

Expression

Bacterial

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAT203

Plasmid

#134880

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYC-Skp-TF

Plasmid

#83921

Purpose

Co-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Trigger Factor

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Sirius/pRSETB

Plasmid

#51956

Purpose

bacterial expression of Sirius, a pH-insensitive and photostable ultramarine fluorescent protein

Depositor

Takeharu Nagai

Article

Tomosugi et al Nat Methods. 2009 May;6(5):351-3. doi: 1

Insert

Sirius

Tags

6xHis

Expression

Bacterial

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pET16b-His-ArgRS

Plasmid

#124104

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Argin+I4 ligase (argS Escherichia coli)

Tags

10x-His

Expression

Bacterial

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBHM2403

Plasmid

#171687

Purpose

pRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.

Depositor

Hiten Madhani

Article

Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

Insert

CnoCAS9

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

TEF1

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SRC_HUMAN_D0

Plasmid

#79700

Purpose

This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

SRC (SRC Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pUC18-mini-Tn7T-Gm-lacZ

Plasmid

#63120

Purpose

mini-Tn7 lacZ transcriptional fusion vector

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Expression

Bacterial

Available Since

May 17, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAWP89

Plasmid

#61264

Purpose

IncP-based replicating vector expressing dTomato under tac promoter.

Depositor

Mary Lidstrom

Article

Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

Insert

Ptac-dTomato

Use

Synthetic Biology

Expression

Bacterial

Promoter

tac

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
mCitrine-pBAD

Plasmid

#54723

Purpose

Localization: Protein Expression Vector , Excitation: 516, Emission: 529

Depositor

Robert Campbell, Michael Davidson, Oliver Griesbeck, Roger Tsien

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Type

Empty backbone

Tags

6xHis and mCitrine

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDIV643

Plasmid

#226182

Purpose

Expresses a color marker (uidA) cassette for disruption on KU70 gene in K. phaffii.

Depositor

Uffe Mortensen

Article

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

uidA marker flanked with targeting regions to KU70 gene in K.phaffii

Expression

Bacterial

Promoter

TEF1p

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-PRDM16 880-1038

Plasmid

#53350

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Shingo Kajimura, Bruce Spiegelman

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 880-1038

Promoter

tac

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
GST-PRDM16 224-454

Plasmid

#53347

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Shingo Kajimura, Bruce Spiegelman

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 224-454

Promoter

tac

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
pET21-Streptavidin-K121R

Plasmid

#89880

Purpose

Streptavidin that has unimpaired biotin binding after dye labeling

Depositor

Mark Howarth

Article

Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

Insert

Streptavidin-K121R-Glutamate_Tag

Tags

6 glutamate tag (C terminal on insert)

Expression

Bacterial

Mutation

K121R

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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