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Plasmid#155180PurposeExpresses colicin E1 residues 1-190DepositorInsertcolicin E1 residues 1-190
UseTagsC-terminal 6x Histidine tagExpressionBacterialMutationtruncation of colicin E1 including 1-190PromoterT7Available sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
nuc-mCherry-pTRIP
Plasmid#163520PurposeUsed to construct lentivirus to express nucleus-targeted mCherry in mammalian cellsDepositorInsertnucleus targeted mCherry
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT-HA-hM3D(Gq)
Plasmid#45547PurposeExpresses hM3D (Gq) neuronal activatorDepositorInserthM3D (Gq)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-26b-NbVHH05-HA-His
Plasmid#171567PurposeBacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)DepositorInsertNbVHH05-HA-His (UBE2J1 Human)
UseTagsHA-tag/His-tag and PelB signal peptideExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET StrepII-StrepII TEV LIC cloning vector (2RRT)
Plasmid#55215DepositorTypeEmpty backboneUseTags2xStrep II and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c-ddcpf1-rfp
Plasmid#153038PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.DepositorInsertAscpf1
UseTagsExpressionBacterialMutationE993APromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3015b
Plasmid#209317PurposeExpresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.DepositorInsertGroup B Streptococcus-compatible single guide RNA
UseTagsExpressionBacterialMutationPromoterXyl/tet promoter that is constitutively activeAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTF-lacZ-rfp
Plasmid#153037PurposeCRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCIRCgp41-1
Plasmid#74227PurposeVector for SICLOPPS-mediated protein circularization using the gp41-1 intein.DepositorTypeEmpty backboneUseSynthetic BiologyTagsgp41-1 C-intein and gp41-1 N-inteinExpressionBacterialMutationPromoterT7-lacAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-26b-NbVHH05-ALFA-His
Plasmid#171569PurposeBacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His)DepositorInsertNbVHH05-ALFA-His (UBE2J1 Human)
UseTagsALFA-tag/His-tag and PelB signal peptideExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10)
Plasmid#55209DepositorTypeEmpty backboneUseTags10xAsn, 10xHis, MPB, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPTDH
Plasmid#66183PurposeExpresses phosphite dehydrogenase in E. coliDepositorInsertPhosphite Dehydrogenase
UseTagsHis6 tagExpressionBacterialMutationE175A, A176RPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET Trx His6 LIC cloning vector (2Tc-T)
Plasmid#37239DepositorTypeEmpty backboneUseTags6xHis and Thioredoxin (Trx)ExpressionBacterialMutationPromoterT7Available sinceAug. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
mem-mCherry-pTRIP
Plasmid#163521PurposeUsed to construct lentivirus to express membrane-targeted mCherry in mammalian cellsDepositorInsertmembrane targeted mCherry
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMP1358
Plasmid#119277Purposefor cloning new sgRNAsDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pJH645
Plasmid#172169PurposeReporter plasmid for pylBCD variant in pBAD backboneDepositorInsertPem7 luxCDA(B.1TAG)E; Plpp pylS; PproK pylT
UseTagsExpressionBacterialMutationInserted amber codon immediately after initiator …PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFCiR2
Plasmid#167512PurposeBroad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, mRFP, Kanamycin Resistance Marker.DepositorInsertCinRAM-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCinAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH462
Plasmid#172154PurposepylBCD variant in pBAD backboneDepositorInsertPara (Sumo-tagged pylBCD); AraC
UseTagsExpressionBacterialMutationSUMO tag fused to N-terminus of PylBPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b-12x
Plasmid#61699PurposeExpresses PTDH mutant Opt12xDepositorInsertOpt12x
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJH643
Plasmid#172168PurposeReporter plasmid for pylBCD variant in pBAD backboneDepositorInsertPara sfGFP.3TAG; Plpp pylS; PproK pylT; AraC; Cam
UseTagsExpressionBacterialMutationsfGFP(N39*, N135*, Y151*)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL103-SpCas9-FLAG-3XNLS
Plasmid#182032PurposeExpression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNPDepositorInsertStreptococcus pyogenes cas9 (cas9 Synthetic)
UseCRISPRTags12X His tag, 3XNLS, FLAG, and MBPExpressionBacterialMutationPromoterT7Available sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
Human USP30 (64-502, construct 8, MG-26-21)
Plasmid#110745PurposeBacterial expression of human USP30 (construct 8)DepositorInsertUSP30 (USP30 Human)
UseTagsN-His6-3C-GST-3CExpressionBacterialMutationF348D, M350D, I353E + insertion deletion: 64-357;…PromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBLego
Plasmid#219967PurposeThe pSBLego vector enables sybodies for the Legobody method with a PelB signal for periplasmic expression and a "SLEHHHHHH" motif for purification and Legobody Fab binding.DepositorTypeEmpty backboneUseTagsSLEHHHHHH and pelB signal sequenceExpressionBacterialMutationPromoterpBADAvailable sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
PET-B-evoSpCas9-NLS-6xHis
Plasmid#207390PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-evoSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationM495V, Y515N, K526E, R661Q, amino acids 1005-1013…PromoterT7Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
SNX32A-c014
Plasmid#175486PurposeProtein expression in bacterial cells. PX domain, S17-M197DepositorInsertSNX32 (SNX32 Human)
UseTagsHis6-SUMOExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
3DAX
Plasmid#51333DepositorInsert3DAX
UseTagsExpressionBacterialMutationTruncation: See CommentsPromoterT7Available sinceFeb. 24, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-Cas9-His
Plasmid#98158PurposePurification of Cas9 proteinDepositorInsertNLS-Cas9-NLS
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEcgRNA
Plasmid#166581PurposeCRISPR-Cas9-assisted genome editing in Escherichia coliDepositorInsertccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-RfxCas13d-His
Plasmid#141322PurposePlasmid for bacterial expression and purification of RfxCas13d proteinDepositorInsertRfx-Cas13d
UseCRISPRTags6xHisExpressionBacterialMutationPromoterT7Available sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSETB/mStayGold
Plasmid#212017PurposemStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.DepositorInsertmStayGold
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b-AavLEA1
Plasmid#53093PurposeAavLEA1 expression (in BL21 DE3 E. coli strain)DepositorInsertAavLEA1
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJune 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
EGFP-pBAD
Plasmid#54762PurposeLocalization: Protein Expression Vector , Excitation: 488, Emission: 507DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags6xHis and EGFPExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCherry-pBAD
Plasmid#54630PurposeLocalization: Protein Expression Vector , Excitation: 587, Emission: 610DepositorTypeEmpty backboneUseTags6xHis and mCherryExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSANG10-3F-BG4
Plasmid#55756PurposeExpresses BG4 scFv anti-G-quadruplex antibody in bacteriaDepositorInsertBG4
UseTags6xHis, pelB leader, and tri-FLAGExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-D4H*-mCherry
Plasmid#134604Purposefluorescent cholesterol-binding probeDepositorInsertD4H*
UseTagsGST and mCherryExpressionBacterialMutationY415A and A463WPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
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Pet28a_Human_H3.1
Plasmid#42631DepositorInsertHuman H3.1 (H3C1 Human)
UseTagsUntaggedExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHEN2-SG4
Plasmid#196071PurposeExpress wild type SG4 in E.coliDepositorInsertSG4
UseTags3XFLAG and 6XHisExpressionBacterialMutationPromoterT7Available sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEcCas
Plasmid#73227PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRNADepositorInsertsCas9
sgRNA
UseCRISPR; Red recombinaseTagsExpressionBacterialMutationPromoternative promoterAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
OmpA3-PNGaseF-TEV-His6
Plasmid#114274PurposeAmidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.DepositorInsertPeptide: N-glycosidase F
UseTagsOmpA3 leader sequence (for periplasmic localisati…ExpressionBacterialMutationPromoterT7Available sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSAM_AraC
Plasmid#91569PurposeMariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.DepositorInsertAraC/PBAD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-BioID2-BioID2-HA
Plasmid#176740PurposeExpresses the promiscuous small biotin ligase, BioID2, in astrocytesDepositorInsertBioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHEN2-SG4 R105A
Plasmid#196072PurposeExpress mutant (R105A) SG4 in E.coliDepositorInsertSG4 R105A
UseTags3XFLAG and 6XHisExpressionBacterialMutationR105A mutation in SG4PromoterT7Available sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas3cRh
Plasmid#133773PurposeExpresses all necessary components of Type I-C CRISPR-Cas systemDepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Pet28a_Human_H4
Plasmid#42633DepositorInsertHuman H4 (H4C1 Human)
UseTagsUntaggedExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
Pet28a_Synthetic_Human_H2A.1
Plasmid#42634DepositorInsertHuman H2A.1 (H2AC13 Human, Synthetic)
UseTagsUntaggedExpressionBacterialMutationCodon OptimizedPromoterT7Available sinceFeb. 25, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
Pet28a_Human_H2B.1
Plasmid#42630DepositorInsertHuman H2B.1 (H2BC8 Human)
UseTagsUntaggedExpressionBacterialMutationPromoterT7Available sinceFeb. 22, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-28a(+)::NL
Plasmid#141289PurposeNLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertNLUC
UseLuciferaseTagsExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPSU1
Plasmid#89439PurposeHigh copy number plasmid 1 to prepare Penn State DNA molecular weight laddersDepositorInsertsUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only