31,059 results
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Plasmid#239127PurposeMinimal centromeric shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker URA3DepositorTypeEmpty backboneUseCentromericExpressionBacterial and YeastAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only
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ReU_pET29b
Plasmid#215426PurposeExpression construct for ReU gene, codon optimized for expression in E. coliDepositorInsertReU
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEM7063
Plasmid#214001PurposePrpsM driven expression of ssrA-tagged eGFP (LVA)DepositorInserteGFP-LVA
ExpressionBacterialPromoterPrpsMAvailable SinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCBD2A-c103
Plasmid#53624Purposebacterial expression plasmidDepositorInsertPCBD2A
TagsHis-6-TEVExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFLxR3
Plasmid#167518PurposeBroad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Tetracycline Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPLuxBAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
MEK1C222
Plasmid#164638PurposeBacterial expression plasmid for His6-MEK1C222DepositorInsertmitogen-activated protein kinase kinase 1 (MAP2K1 Human)
TagsHis6-tagExpressionBacterialMutationG(-19)F/S222C/C277S/C376SPromoterT7Available SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-TT (TetR, P21)
Plasmid#45989DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDR111_Bgal no NLS
Plasmid#188397PurposeExpresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.DepositorInsertlacZ
TagsPhoD secretion peptideExpressionBacterialPromoterPhyper spankAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-KRaION1
Plasmid#177799PurposeBacterial expression of green fluorescent potassium indicator KRaION1DepositorInsertKRaION1
ExpressionBacterialPromoteraraBAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGT-Ah6
Plasmid#122573PurposeMAGIC donor plasmid (Himar transposon with catP/sfGFP payload)DepositorInsertcatP/sfGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCK306
Plasmid#110544PurposerhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.DepositorInsertsPrhaBAD
yfp
rhaS
ExpressionBacterialPromoterN/A, PrhaBAD, and kanR promoter (upstream of kanR…Available SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-NTOM20-mVenus-Zdk2
Plasmid#81011PurposeExpression of Zdk2 anchored to mitochondriaDepositorInsertZdk2
Tags6x His tag, FLAG, NTOM20, and mVenusExpressionBacterial, Insect, and Mamm…Available SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
human p53-(82-393)
Plasmid#24867DepositorAvailable SinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
CeNL_pRSETb
Plasmid#89510PurposeCyan color variant of bright luminescent protein for bacterial expressionDepositorInsertCeNL
TagsHis X 6ExpressionBacterialPromoterT7Available SinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTF-lacZ-rfp
Plasmid#153037PurposeCRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.DepositorInsertCRISPR array
ExpressionBacterialAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTR47m4-GFP
Plasmid#102436PurposeFormaldehyde biosensor plasmid with sGFP reporter and modified frm promoterDepositorInsertsFormaldehyde repressor protein (frmR)
Superfolder GFP
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL1749 (pDonor, R*MmeI)
Plasmid#160733PurposeMini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.DepositorInsertVchCAST donor DNA
ExpressionBacterialPromoterN/AAvailable SinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag
Plasmid#109030PurposeWild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
TagsFlag TagExpressionBacterialPromoterT7Available SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
1B-GFP1-10
Plasmid#193867PurposeE. coli expression vector for Hisx6 tagged GFP1-10.DepositorInsertsfGFP1-10
Tags6xHisExpressionBacterialPromoterT7 PromoterAvailable SinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGT-B2
Plasmid#122580PurposeMAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)DepositorInsertsfGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTP1174
Plasmid#104162PurposeBacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)DepositorInsertAnti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEC2483 (pSpy1C)
Plasmid#220248Purposereplicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expressionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP1187
Plasmid#119256Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
(SIM)10
Plasmid#126946PurposeBacterial expression plasmid for (SIM)10 client to assess SUMO-SIM scaffolds.DepositorInsert10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)
ExpressionBacterialPromoterunknownAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXY027
Plasmid#98915PurposeExpress FtsZ tagged with GFP in E coliDepositorInsertFtsZ
TagsGFPExpressionBacterialPromoterT5-lacAvailable SinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15bHis6Tnp
Plasmid#79807PurposeTransform BL21 Gold(DE3) to induce the expression of HisTnp hyperactive mutationsDepositorInsertHis6Tnp
TagsHisExpressionBacterialAvailable SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
Fusion of Zika NS2B-NS3 proteins
Plasmid#204815PurposeBacterial expression of a fusion of Zika NS2B-NS3 proteinsDepositorInsertZika NS2B-NS3 fusion (orf1ab Zika)
ExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD/myc-his A Rv0712 (FGE)
Plasmid#16132PurposeBacterial expression of Mycobacterium tuberculosis FGEDepositorInsertFormylglycine-generating enzyme
ExpressionBacterialAvailable SinceNov. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-mCherry
Plasmid#127058PurposeLow phosphate inducible mCherry with N-terminal linker and His tagDepositorInsertmCherry
ExpressionBacterialPromoteryibDpAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-TRIM28
Plasmid#45570DepositorAvailable SinceMay 29, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSL0283 (pTnsABC)
Plasmid#130633PurposeExpresses V. cholerae CAST TnsA, TnsB, and TnsC from a T7 promoter.DepositorInsertVchTnsA, VchTnsB, VchTnsC
UseCRISPR; TransposonExpressionBacterialPromoterT7Available SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-FLAG
Plasmid#119759PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal FLAG tag.DepositorTypeEmpty backboneTagsFLAG and GSTExpressionBacterialPromotertac promoterAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGT-B3
Plasmid#122581PurposeMAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)DepositorInsertsfGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRS-LacI-GFP
Plasmid#220198Purposemini-Tn7 GFP expression vector - LacI/pLac promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterLacI/pLacAvailable SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL0527 (pDonor)
Plasmid#130634PurposeEncodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.DepositorInsertVchCAST donor DNA (Mini-Tn)
UseCRISPR; TransposonExpressionBacterialAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHMM
Plasmid#67717PurposeExpression vector MBP/hexahistidine tagged variant of the MS2 coat proteinDepositorInsertHexahistidine/Maltose binding protein/MS2 coat protein fusion
TagsHexahistidine tagExpressionBacterialPromoterT7 RNA polymeraseAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACYC-TtCas6-4xcrRNA4.5
Plasmid#127764PurposeFor expression of T. thermophilus Csm complex in E. coli (crRNA)DepositorInsertsSynthetic CRISPR array containing four identical T. thermophilus spacers
Cas6A
ExpressionBacterialMutationCodon optimized for expression in E. coliPromoterT7Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
mPapaya-pBAD
Plasmid#54838PurposeLocalization: Protein Expression Vector , Excitation: 530, Emission: 541DepositorTypeEmpty backboneTags6xHis and mPapayaExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJC175e-DddI
Plasmid#179693PurposeMutagenesis plasmid with constitutive bacterial expression of DddI immunity proteinDepositorInsert[SD8] gIII
ExpressionBacterialAvailable SinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1 TIM9,10
Plasmid#170280PurposeExpresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli productionDepositorInsertExpressionBacterialPromoterTim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…Available SinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only