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32,069 results

Showing: 3721 - 3760 of 32069 results

  1. pMD19T-slr0230-PL22-sgRNANT1-KmR

    Plasmid
    #73224
    Purpose
    Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli
    Depositor
    Paul Hudson
    Article
    Yao et al ACS Synth Biol. 2015 Dec 28.
    Insert
    PL22-sgRNA NT1
    Expression
    Bacterial
    Promoter
    PL22
    Available Since
    April 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73224-plasmid-map-sequence-id-135053

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  14. pKD4_mCherry

    Plasmid
    #187386
    Purpose
    To chromosomally tag genes of interest with mCherry. FRT-mCherry-kan-FRT
    Depositor
    Roi Avraham
    Insert
    mCherry
    Expression
    Bacterial
    Available Since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    187386-plasmid-map-sequence-id-370396

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  23. BRD4

    Plasmid
    #38943
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRD4 (BRD4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    38943-plasmid-map-sequence-id-456555

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  25. pcDNA3.1.Gαi2-LgB91

    Plasmid
    #134358
    Purpose
    Nanoluc complementation assay. Expression of Gαi2 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi2. Addition of the HA epitope at N terminus of Gαi2.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gαi2-LgB91 (GNAI2 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134358-plasmid-map-sequence-id-306397

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  26. pET28-SpRY-NLS-6xHis (gbr2101)

    Plasmid
    #181743
    Purpose
    bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    bacterial codon optimized SpRY
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS(SV40)-6xHis and MKIEE MBP tag
    Expression
    Bacterial
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    T7
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181743-plasmid-map-sequence-id-354675

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  27. pET30a-Tn5

    Plasmid
    #127917
    Purpose
    Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.
    Depositor
    Silin Zhong
    Article
    Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:
    Insert
    Tn5
    Tags
    Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Mutation
    E54K, L372P
    Promoter
    T7
    Available Since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    127917-plasmid-map-sequence-id-254855

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  28. pKOS6b-KOpstS

    Plasmid
    #237503
    Purpose
    Knockout of pstS
    Depositor
    Buz Barstow
    Insert
    phosphate ABC transporter substrate-binding protein PstS
    Expression
    Bacterial
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237503-plasmid-map-sequence-id-483088

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  29. pKOS6b-URP112

    Plasmid
    #237504
    Purpose
    Upregulation of mgdh with P112
    Depositor
    Buz Barstow
    Insert
    membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family
    Expression
    Bacterial
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237504-plasmid-map-sequence-id-475903

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  33. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

    Plasmid
    #215769
    Purpose
    encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus
    Depositor
    Dimitrios Wagner
    Article
    Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:
    Inserts
    TRAC-5' homology arm (TRAC Human)
    P2A-CD19.28.zeta-bgH (CD19 Bovine, Mouse, Human)
    TRAC-3' homology arm (TRAC Human)
    Expression
    Bacterial
    Mutation
    Cas12a PAM mutated
    Available Since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    215769-plasmid-map-sequence-id-435524

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  35. pGP-Tn7-PcL

    Plasmid
    #225281
    Purpose
    Mini-Tn7 pGP-Tn7-Cm vector with constitutive PcL promoter for the constitutive expression of target genes inserted into the attTn7 chromosomal site.
    Depositor
    Mark Schembri
    Article
    Chang et al Appl Environ Microbiol. 2024 Oct 23;90(1
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    Constitutive PcL promoter
    Available Since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    225281-plasmid-map-sequence-id-457263

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  36. pGP-Tn7-PrpsM

    Plasmid
    #225282
    Purpose
    Mini-Tn7 pGP-Tn7-Cm vector with constitutive PrpsM promoter for the constitutive expression of target genes inserted into the attTn7 chromosomal site.
    Depositor
    Mark Schembri
    Article
    Chang et al Appl Environ Microbiol. 2024 Oct 23;90(1
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    Constitutive PrpsM promoter
    Available Since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    225282-plasmid-map-sequence-id-457262

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  37. p406ADH1

    Plasmid
    #15974
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    ADH1 promoter + polylinker + CYC1 terminator (ADH1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    ADH1 promoter is between SacI and XbaI restrictio…
    Available Since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15974-plasmid-map-sequence-id-8459

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Showing: 3721 - 3760 of 32069 results