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Showing: 3721 - 3760 of 29646 results

  2. pDest-Sumo

    Plasmid
    #106980
    Purpose
    LR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tag
    Depositor
    Han Choe
    Article
    Nguyen et al Sci Rep. 2017 Nov 23;7(1):16139. doi: 10
    Type
    Empty backbone
    Use
    Tags
    His8-Sumo
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    106980-plasmid-map-sequence-id-204719

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  5. pcDNA3.1.Gαi3-LgB91

    Plasmid
    #134359
    Purpose
    Nanoluc complementation assay. Expression of Gαi3 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi3. Addition of the HA epitope at N terminus of Gαi3.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gαi3-LgB91 (GNAI3 Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134359-plasmid-map-sequence-id-306392

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  6. SiriusGFP

    Plasmid
    #123201
    Purpose
    We have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.
    Depositor
    Joseph Santos-Sacchi
    Article
    Zhong et al J Neurosci Methods. 2019 Feb 1;313:68-76
    Insert
    SiriusGFP
    Use
    Mouse Targeting
    Tags
    N/A
    Expression
    Bacterial and Mammalian
    Mutation
    eGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…
    Promoter
    CMV
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    123201-plasmid-map-sequence-id-239192

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  8. pDIV515

    Plasmid
    #177703
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    tRNA-sgRNA-tRNA
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…
    Available since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177703-plasmid-map-sequence-id-353317

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  10. pET24a MDH_AQUAR

    Plasmid
    #204239
    Purpose
    Bacterial expression of A. arcticum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_AQUAR
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204239-plasmid-map-sequence-id-413339

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  15. LACTB2

    Plasmid
    #39115
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    LACTB2 (LACTB2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  21. pBA1015

    Plasmid
    #189589
    Purpose
    T7gp1.7 deletion HR vector 250bp HR Arms (T7 phage editing)
    Depositor
    Jennifer Doudna
    Article
    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.
    Insert
    T7gp1.7 Locus 250bp Homology Arms
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Encodes for a full deletion of T7gp1.7
    Promoter
    Available since
    Aug. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    189589-plasmid-map-sequence-id-370607

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  23. pJMP3653

    Plasmid
    #222348
    Purpose
    Expression vector with PabstBR promoter, kanR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.
    Depositor
    Jason Peters
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    222348-plasmid-map-sequence-id-439082

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  36. pSLC-238

    Plasmid
    #73192
    Purpose
    Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (chpB) under the control of rhamnose induceable promoter (PrhaB) for negative selection.
    Depositor
    Swaine Chen
    Article
    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8
    Insert
    PrhaB-chpB (chpB )
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaB
    Available since
    June 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    73192-plasmid-map-sequence-id-151373

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  39. p006-GFP-pBAD

    Plasmid
    #108315
    Purpose
    GFP from jellyfish Aquorea sp. under pBAD promoter with AraC repressor; kanamycin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    GFP under control of arabinose-inducible promoter pBAD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Aug. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    108315-plasmid-map-sequence-id-218821

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Showing: 3721 - 3760 of 29646 results