32,069 results
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Plasmid#188454PurposeExpress in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.DepositorAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only
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pJS167
Plasmid#48881PurposePositive feedback plasmid from oscillatorDepositorInsertsaraC gene with ssrA tag
monomeric yeast-enhanced green fluorescent protein
UseSynthetic BiologyTagsssrA (TSAANDENYALAA)ExpressionBacterialPromoterPara/lacAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
HSD11B1
Plasmid#39155PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1_4xUb
Plasmid#199779PurposeUsed for the expression of 4X human ubiquitin in tandem (SMC interal no. 538)DepositorInsert4X Ubiquitin in Tandem
TagsGSTExpressionBacterialAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJ1996_v2
Plasmid#140664PurposedCas9 & Csy4DepositorInsertdCas9 & Csy4
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP3
Plasmid#79875PurposeBacillus subtilis sgRNA expression vector; integrates into thrCDepositorInsertsgRNA RR1
UseCRISPRExpressionBacterialPromotervegAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECJ3
Plasmid#75465PurposeToggle switch plasmid containing lacI-pdt3DepositorInsertlacI-pdt3
ExpressionBacterialPromoterpLtetOAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYTK069
Plasmid#65176PurposeEncodes ConR3 as a Type 5 part to be used in the Dueber YTK systemDepositorInsertConR3
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAPB02
Plasmid#65628PurposeA transformation-associated recombination (TAR) based genomic capture and expression vector for antibiotic production in B. subtilisDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
p15aC-GFP
Plasmid#107862PurposeControl plasmid expressing GFPDepositorInsertGFP
TagsmycExpressionBacterialPromoterproDAvailable SinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
bactin:GFP-LAP2b
Plasmid#105980Purposetransgenesis, ubiquitous expressionDepositorInsertLAP2b
TagsGFPExpressionBacterialAvailable SinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-hsf-1-sgRNA-B
Plasmid#177787PurposeOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)DepositorInserthsf-1 (hsf-1 Nematode)
ExpressionBacterialAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDule2 - Mj Acd A9
Plasmid#197652PurposeE. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pairDepositorInsertsMj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA
TagsNoneExpressionBacterialMutationY32A, L65D, F108L, Q109E, D158S, L162TPromoterGlnS and lppAvailable SinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
plRL118
Plasmid#163632PurposeRenilla luciferase reporter plasmid; Giles integrating.DepositorTypeEmpty backboneUseLuciferaseExpressionBacterialPromoterMycobacterial Optimized Promoter (MOP)Available SinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDAI-SceI-pheS
Plasmid#111611PurposeYeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.DepositorInsertpheS
ExpressionBacterialAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL1-GFP
Plasmid#180420PurposeKan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a GFP promoter.DepositorInsertGFP
ExpressionBacterialAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAWPSG-Po-nCas9-BE
Plasmid#195740PurposeExpress APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coliDepositorArticleInsertDi-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPo (phoenol-inducible promoter)Available SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pN3
Plasmid#233520PurposeNb Vector Plasmid (under galactose induction), AmpR (E. coli) and HygroR (yeast), Cen6/ARSDepositorTypeEmpty backboneExpressionBacterial and YeastPromoterpGAL1Available SinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
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poly H35S SUMO1
Plasmid#221071PurposeExpression of poly H35S SUMO1 protein under T7 promoterDepositorInsertFive repeats of H35S SUMO1 each separated by (GGS)4 (SUMO1 Human)
TagsHis and MBPExpressionBacterialMutationH35SPromotertacAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
polySIM
Plasmid#221068PurposeExpression of polySIM protein under T7 promoterDepositorInsert10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4
TagsHis and MBPExpressionBacterialPromoterunknownAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pCDFDuet-1:ChrH-ChrI
Plasmid#225083PurposeExpresses multinuclear non-heme iron dependent oxidative enzyme ChrH and partner protein ChrI in E.coliDepositorInsertChrH
ExpressionBacterialMutationWTPromoterT7Available SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Himar-155
Plasmid#185939PurposeHimar transposon delivery plasmid that delivers a red fluorescent mScarlet-I protein geneDepositorInsertmScarlet-I
ExpressionBacterialAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Himar-157
Plasmid#185940PurposeHimar transposon delivery plasmid that delivers a green fluorescent mClover3 protein geneDepositorInsertmClover3
ExpressionBacterialAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Himar-143
Plasmid#185932PurposeHimar transposon delivery plasmid that delivers a yellow fluorescent sYFP2 protein geneDepositorInsertsYFP2
ExpressionBacterialAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pY001 (pFnCpf1_full)
Plasmid#69973PurposeExpresses FnCpf1 locus including Cas genes and spacers 1-4 of CRISPR array.DepositorInsertFnCpf1 locus
UseCRISPRExpressionBacterialAvailable SinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET19b+H3
Plasmid#157807PurposeExpression of cysteine-free human histone H3 (c111A)DepositorInsertH3
ExpressionBacterialMutationC111APromoterT7Available SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-BirA-eCPX-AP
Plasmid#121907PurposeExpresses BirA and eCPX-APDepositorInsertsTags6xHisExpressionBacterialPromoterT7 and pBAD promoterAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5371
Plasmid#128732PurposeGateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal Cya tagDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI
Plasmid#115267PurposeExpresses LbuCas13a from a SUMO-tagged bacterial expression constructDepositorInsertLbuCas13a
TagsSUMOExpressionBacterialAvailable SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMVV099
Plasmid#215305PurposeExpresses sfGFP under induction with the Cuminic Acid Marionette Cassette (CymRAM + PCymRC-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialPromoterPCymRCAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.AT110
Plasmid#135480PurposeE. coli periplasmic expression of conformational nanobody Nb.AT110 recognizing angiotensin II type 1 receptorDepositorInsertNanobody AT110
Tags6xHis-tag and pelB leaderExpressionBacterialPromoterT7Available SinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMyBADNT-kan
Plasmid#84691PurposeProtein expression in mycobacteriaDepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromoterAraCAvailable SinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UnaG
Plasmid#203485PurposeFor recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.DepositorInsertUnaG
ExpressionBacterial and PlantAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM20C
Plasmid#135487PurposeAgrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hphDepositorInserthph SpunH2A/Bpr H2B-tdTomato
TagstdTomatoExpressionBacterial and YeastPromoterSpunH2A/Bpr (divergent)Available SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFEndoNA2
Plasmid#174667PurposeExpresses GFP-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.DepositorInsertGFP-endoNA2 fusion protein
TagsHistagExpressionBacterialPromoterT5Available SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
SARS_pNIC-CTB10H
Plasmid#153056PurposeExpression construct for producing Avi tagged Seryl-tRNA synthetaseDepositorAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYC-GFPM2
Plasmid#102791PurposeExpresses GFP under WT T7 promoterDepositorInsertGreen Fluorescent Protein
ExpressionBacterialAvailable SinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only