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5,038 results

Showing: 651 - 700 of 5038 results

  4. pDest-633

    Plasmid
    #161877
    Purpose
    Standard Gateway (attR1-attR2) vector for expression of His6-GST fusions using the Bac-to-Bac insect cell system
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Tags
    His6-GST
    Expression
    Insect
    Available Since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    161877-plasmid-map-sequence-id-327078

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  7. pSN840

    Plasmid
    #184832
    Purpose
    Expresses human KIF5A(āˆ†exon27) and human KLC1 in insect cells
    Depositor
    Kyoko Chiba
    Article
    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g
    Inserts
    KIF5A (āˆ†exon27) (KIF5A Human)
    KLC1 (KLC1 Human)
    Use
    Cre/Lox
    Tags
    His-FLAG and mScarlet-2xStrepII
    Expression
    Insect
    Mutation
    āˆ†exon27 form of human KIF5A
    Promoter
    p10 and polyhedrin
    Available Since
    July 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    184832-plasmid-map-sequence-id-367218

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  12. pAcGP67A-TfR

    Plasmid
    #12130
    Depositor
    Pamela Bjorkman
    Article
    Lebron et al Cell. 1998 Apr 3. 93(1):111-23.
    Insert
    transferrin receptor (TFRC Human)
    Tags
    6X-His tag, Factor Xa cleavage site, and leader pā€¦
    Expression
    Insect
    Mutation
    The portion of the human TfR gene encoding residuā€¦
    Available Since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only

  14. pUASTattb-cAMPr Y205A, R739A

    Plasmid
    #84933
    Purpose
    cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity.
    Depositor
    Justin Blau
    Article
    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/
    Insert
    cAMPr Y205A R739A
    Expression
    Insect
    Mutation
    PKA-C Y205A, PKA-R R739A
    Promoter
    5X UAS
    Available Since
    March 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    84933-plasmid-map-sequence-id-180303

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  16. ORCO-T2A-QF-DBD

    Plasmid
    #162525
    Purpose
    Plasmid for CRISPR-mediated knock-in line that expresses the DNA-binding domain of QF by knocking-in a T2A-QF-DBD fragment replacing the stop codon of the endogenous Aedes aegypti Orco gene
    Depositor
    Leslie Vosshall
    Article
    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.
    Insert
    Orco-left-HDR-arm; T2A-Zip-::QFDBD-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
    Expression
    Insect
    Available Since
    Aug. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    162525-plasmid-map-sequence-id-354642

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  17. PA-DAD-wt

    Plasmid
    #53095
    Purpose
    Expresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.
    Depositor
    Klaus Hahn, Ronen Zaidel-Bar
    Article
    Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3
    Insert
    LOV(wt)-6aa-DAD
    Tags
    6x His and mVenus
    Expression
    Bacterial, Insect, and Mammā€¦
    Mutation
    L47F and C49S in Venus
    Available Since
    Aug. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    53095-plasmid-map-sequence-id-119520

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  18. pFastBac1 human full-length CENP-E-mNeon-6xHis

    Plasmid
    #180484
    Purpose
    pFastBac1 human CENP-E-mNeon-His, containing a 3c protease cleavage site between mNeon and His tag. Codon optimized for expression in Sf9 cells
    Depositor
    Julie Welburn
    Article
    Craske et al Open Biol. 2022 Mar;12(3):210389. doi: 1
    Insert
    CENP-E (CENPE Human, human, Synthetic)
    Tags
    mNeon-6xHis
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    180484-plasmid-map-sequence-id-377471

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  24. pDest-795

    Plasmid
    #161881
    Purpose
    Standard Gateway (attR1-attR2) vector for expression of maltose-binding protein (MBP) fusions using the Bac-to-Bac insect cell system
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Tags
    MBP
    Expression
    Insect
    Available Since
    Jan. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    161881-plasmid-map-sequence-id-322112

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  29. pFastBac1-Nucleocapsid

    Plasmid
    #179696
    Purpose
    Expresses H6 tagged SARS CoV-2 nucleocapsid in Sf9 cells
    Depositor
    Mark Arbing
    Insert
    SARS-CoV-2 Nucleocapsid (N protein) (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    His6-TEV protease site
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    179696-plasmid-map-sequence-id-352199

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  34. ppBAalbNixE1E2 PUb-GFP

    Plasmid
    #173667
    Purpose
    transgenesis vector for Aedes albopictus ectopic expression of the Nix gene
    Depositor
    Eric Marois
    Article
    Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1
    Inserts
    Nix masculinisation gene
    EGFP
    Expression
    Insect
    Mutation
    Nix isoforms with only exons 1, 2 separated by inā€¦
    Promoter
    Aedes aegypti Polyubiquitin and cloned Nix promotā€¦
    Available Since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    173667-plasmid-map-sequence-id-342953

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  37. pBac[3xP3-EGFP;Tc'hsp5'-Gal4Delta-3'UTR]

    Plasmid
    #86449
    Purpose
    pBAc transposon with transformation marker for Tribolium transgenesis; Drives EGFP expression in the eyes; contains Gal4_ driven by Tribolium heatshock promoter; (Transformation plasmid)
    Depositor
    Gregor Bucher
    Insert
    3xP3-EGFP;Tc'hsp5'-Gal4Delta-3'UTR
    Expression
    Insect
    Available Since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86449-plasmid-map-sequence-id-171154

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  41. pFB-LIC-Bse

    Plasmid
    #26108
    Purpose
    SGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection
    Depositor
    Opher Gileadi
    Article
    Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    His6 - TEV
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    Oct. 4, 2010
    Availability
    Academic Institutions and Nonprofits only
    26108-plasmid-map-sequence-id-12260

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  46. pDR125

    Plasmid
    #37150
    Depositor
    Dale Ramsden
    Insert
    lig4 (LIG4 Human)
    Tags
    H6
    Expression
    Insect
    Available Since
    July 18, 2012
    Availability
    Academic Institutions and Nonprofits only
Showing: 651 - 700 of 5038 results