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5,038 results

Showing: 901 - 950 of 5038 results

  1. Dynein-2_complex

    Plasmid
    #132536
    Purpose
    Full length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.
    Depositor
    Andrew Carter
    Article
    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1
    Inserts
    DYNC2H1 (DYNC2H1 Human)
    DYNC2LI1 (DYNC2LI1 Human)
    WDR34 (WDR34 Human)
    WDR60 (WDR60 Human)
    DYNLL1 (DYNLL1 Human)
    DYNLL2 (DYNLL2 Human)
    DYNLRB1 (DYNLRB1 )
    DYNLRB2 (DYNLRB2 Human)
    DYNLT1 (DYNLT1 Human)
    TCTEX1D2 (TCTEX1D2 Human)
    DYNLT3 (DYNLT3 Human)
    LC8-like (LOC392067 Human)
    Tags
    8x His, Linker-TEV-linker-TEV, SNAPf, Strep, and …
    Expression
    Insect
    Mutation
    Codon optimised for Sf9 expression
    Available Since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    132536-plasmid-map-sequence-id-270901

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  5. pKM50

    Plasmid
    #123656
    Purpose
    Encodes Renilla luciferase under the Aedes aegypti ubiquitin UbL40 promoter for constitutive expression in mosquito cells
    Depositor
    Kevin Maringer
    Article
    Fredericks et al PLoS Negl Trop Dis. 2019 Nov 6;13(11):e0
    Insert
    Aedes aegypti UbL40 promoter
    Use
    Luciferase
    Expression
    Insect
    Promoter
    UbL40 promoter
    Available Since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    123656-plasmid-map-sequence-id-243712

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  15. pOCC154

    Plasmid
    #118902
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    Tags
    TwinStrep, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118902-plasmid-map-sequence-id-232325

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  20. pCoofy64

    Plasmid
    #122016
    Purpose
    Baculovirus vector for parallel SLIC cloning containing gp67 signal sequence, N-terminal His6-Smt3Star (SumoStar*) and different C-tag options
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Tags
    His6-Smt3Star and Multi3: depending on the LP2 pr…
    Expression
    Insect
    Available Since
    May 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    122016-plasmid-map-sequence-id-243594

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  21. pGEM-3XP3.RFP-tan

    Plasmid
    #83474
    Purpose
    RFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.
    Depositor
    Patricia Wittkopp
    Article
    Lamb et al Fly (Austin). 2016 Aug 5:1-12.
    Insert
    3XP3.RFP-tan
    Use
    CRISPR
    Expression
    Bacterial and Insect
    Promoter
    3XP3 eye-expression Promoter
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    83474-plasmid-map-sequence-id-159168

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  24. pKSB- 3xP3-YFPsv40 GGGG AAGA

    Plasmid
    #174530
    Purpose
    3xP3-YFP fluorescence marker cassette for gene knock-in in mosquitoes
    Depositor
    Eric Marois
    Insert
    3xP3-YFP BsaI cassette
    Expression
    Insect
    Promoter
    3xP3
    Available Since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    174530-plasmid-map-sequence-id-343225

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  26. AAEL006511-Cas9

    Plasmid
    #100580
    Purpose
    The promoter of AAEL006511 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti
    Depositor
    Omar Akbari
    Article
    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p
    Inserts
    AAEL006511-hspcas9-GFP
    Opie2-dsRed
    Use
    CRISPR
    Tags
    GFP and dsRED
    Expression
    Insect
    Promoter
    AAEL006511 and Opie2
    Available Since
    Oct. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    100580-plasmid-map-sequence-id-193175

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  28. pTriEx-PA-Rac1-I539E

    Plasmid
    #22026
    Purpose
    Expression of photoactivatable Rac1 ('lit' state mutant)
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1-I539E (RAC1 Avena sativa (oat), Human)
    Tags
    6X His
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV domain contains 'lit state' mutatio…
    Promoter
    CMV, p10
    Available Since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only

  29. pTriEx-PA-Rac1-C450A

    Plasmid
    #22025
    Purpose
    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
    Tags
    6X His
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV domain contains light-insensitive mutation C4…
    Promoter
    CMV, p10
    Available Since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only

  30. RABEP1A-c019

    Plasmid
    #175477
    Purpose
    Protein expression in insect cells. Entire CDS, M1-T862. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    RABEP1 (RABEP1 Human)
    Tags
    TEV-TwinStrep
    Expression
    Insect
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175477-plasmid-map-sequence-id-353250

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  32. pE4c

    Plasmid
    #17464
    Purpose
    modified pENTR vector for C-terminal GST tag fusion with your gene of interest
    Depositor
    Giovanna Benvenuto
    Article
    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.
    Type
    Empty backbone
    Use
    Modified gateway entry vector
    Tags
    GST
    Expression
    Bacterial, Insect, Mammalia…
    Available Since
    March 21, 2008
    Availability
    Academic Institutions and Nonprofits only
    17464-plasmid-map-sequence-id-8632

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  37. pOET5 rota-VLP

    Plasmid
    #218147
    Purpose
    Forms rotavirus-like particles in insect cells by expression of VP6 (GenBank ID: ACL93331.1).
    Depositor
    Minna Hankaniemi
    Insert
    Rotavirus VP6
    Expression
    Insect
    Promoter
    Polyhedrin
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218147-plasmid-map-sequence-id-433323

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  38. pOET5 CVB3-VLP

    Plasmid
    #218146
    Purpose
    Forms coxsackievirus 3-like particle in insect cells by expression of VP1–VP4 polyprotein (GeneID M33854.1) and 3CD protease (GeneID M33854.1).
    Depositor
    Minna Hankaniemi
    Inserts
    VP1–VP4
    3CD
    Expression
    Insect
    Promoter
    CMV and Polyhedrin
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218146-plasmid-map-sequence-id-433321

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  39. pOET5 GII.4 Sydney noro-VLP

    Plasmid
    #218092
    Purpose
    Forms Sydney GII.4 Sydney norovirus-like particle in insect cells by expression of VP1 from strain GII.4/Sydney/NSW0514/2012/AU (GenBank ID: AFV08795).
    Depositor
    Minna Hankaniemi
    Insert
    noro-VLP
    Expression
    Insect
    Promoter
    Polyhedrin
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218092-plasmid-map-sequence-id-433318

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  41. pE3c

    Plasmid
    #17463
    Purpose
    modified pENTR vector for C-terminal 6x MYC tag fusion with your gene of interest
    Depositor
    Giovanna Benvenuto
    Article
    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.
    Type
    Empty backbone
    Use
    Modified gateway entry vector
    Tags
    6xMyc
    Expression
    Bacterial, Insect, Mammalia…
    Available Since
    March 21, 2008
    Availability
    Academic Institutions and Nonprofits only
    17463-plasmid-map-sequence-id-8631

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  42. pOCC304

    Plasmid
    #118897
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with TEV and C-terminal HIS6, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-TwinStrep-MBP-TEV-NotI-ccdB-AscI-TEV-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with TEVprotease and TwinStrep-MB…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118897-plasmid-map-sequence-id-232369

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  47. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7f-T2A-tdTomato-p10

    Plasmid
    #172915
    Purpose
    Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Insert
    jGCaMP7f-T2A-tdTomato
    Tags
    6xHis and T2A-tdTomato
    Expression
    Insect
    Promoter
    hsp70
    Available Since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    172915-plasmid-map-sequence-id-341221

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  48. VanGlow-RR-DSCP

    Plasmid
    #83339
    Purpose
    for Gateway cloning of enhancer elements upstream of the DSCP and mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.
    Depositor
    Cheng-Yu Lee
    Article
    Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    mCherry::Renilla(nls)
    Expression
    Insect
    Promoter
    Drosophila Synthetic Core Promoter
    Available Since
    Dec. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    83339-plasmid-map-sequence-id-158654

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Showing: 901 - 950 of 5038 results