75,914 results

1. pMD2.G

   Plasmid

   #12259

   Purpose

   VSV-G envelope expressing plasmid

   Depositor

   Didier Trono

   Insert

   VSV G
   

   Use

   Lentiviral; Envelope

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. psPAX2

   Plasmid

   #12260

   Purpose

   2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)

   Depositor

   Didier Trono

   Type

   Empty backbone

   Use

   Lentiviral; Packaging

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9(BB)-2A-GFP (PX458)

   Plasmid

   #48138

   Purpose

   Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiCRISPRv2-mCherry

   Plasmid

   #99154

   Purpose

   Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.

   Depositor

   Agata Smogorzewska

   Inserts

   Cas9
   mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiCRISPR v2

   Plasmid

   #52961

   Purpose

   Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-VSV-G

   Plasmid

   #8454

   Purpose

   Envelope protein for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral).

   Depositor

   Bob Weinberg

   Article

   Stewart et al RNA 2003 Apr;9(4):493-501.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW57-CMV-ssRFP-GFP-KDEL

   Plasmid

   #128257

   Purpose

   Fluorescence reporter to monitor ER-phagy activity

   Depositor

   Noboru Mizushima

   Article

   Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

   Insert

   ssRFPGFPKDEL
   

   Use

   Lentiviral

   Tags

   RFPGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP

   Plasmid

   #176015

   Purpose

   Mammalian expression of EGFP. Parton lab clone DFS

   Depositor

   Rob Parton

   Article

   Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSV-Rev

   Plasmid

   #12253

   Purpose

   3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)

   Depositor

   Didier Trono

   Article

   Dull et al J Virol. 1998 Nov . 72(11):8463-71.

   Insert

   Rev (rev )
   

   Use

   Lentiviral; Packaging

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti CMV Puro LUC (w168-1)

    Plasmid

    #17477

    Purpose

    3rd gen lentiviral Luciferase expression vector, CMV promoter, Puro

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. PRS_8-ChrimsonR-tdTomato

    Plasmid

    #192587

    Purpose

    AAV-vector for optogenetic activation of noradrenergic neurons

    Depositor

    Simon Wiegert

    Article

    Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

    Insert

    ChrimsonR
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    PRSX8

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiGuide-Puro

    Plasmid

    #52963

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Cloning Grade DNA

    Inserts

    S. pyogenes sgRNA cassette
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-a and hU6

    Available since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3-FLAG-human NPM1 wt

    Plasmid

    #160692

    Purpose

    Mammalian expression of FLAG-tagged NPM1

    Depositor

    Charles Sherr

    Article

    Bertwistle et al Mol Cell Biol. 2004 Feb . 24(3):985-96.

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV2/2

    Plasmid

    #104963

    Purpose

    AAV packaging plasmid expressing Rep/Cap genes for production of serotype 2

    Depositor

    Melina Fan

    Insert

    Rep2/Cap2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p5

    Available since

    Oct. 2, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. v5 eVLP gag-pro-pol

    Plasmid

    #228466

    Purpose

    Expresses MMLVgag-pro-pol(Q226P) for producing v5 eVLPs

    Depositor

    David Liu

    Article

    Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

    Insert

    MMLVgag-pro-pol(Q226P)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q226P

    Promoter

    CMV

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiMPH v2

    Plasmid

    #89308

    Purpose

    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Insert

    MS2-P65-HSF1_2A_Hygro (RELA Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1a

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUAS-NanoLuc

    Plasmid

    #87696

    Purpose

    Gal4VP16 driven expression of NanoLuc

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    NanoLuc
    

    Use

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. CRISPRoff-v2.1

    Plasmid

    #167981

    Purpose

    Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter

    Depositor

    Luke Gilbert

    Article

    Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

    Insert

    CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
    

    Use

    Tags

    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV2/5

    Plasmid

    #104964

    Purpose

    AAV packaging plasmid expressing Rep/Cap genes for production of serotype 5

    Depositor

    Melina Fan

    Insert

    Rep2/Cap5
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p5

    Available since

    Oct. 2, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pU6-tevopreq1-GG-acceptor

    Plasmid

    #174038

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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