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Mammalian Expression

76,300 results

Showing: 7101 - 7150 of 76300 results

  1. pBA950

    Plasmid
    #122239
    Purpose
    CROP-seq vector optimized for sgRNA expression and CRISPRi activity. This vector expresses a eGFP-NT2 sgRNA with modified U6 promotor and sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    BFP selectable marker, plus a modified mU6 promoter and sgRNA constant region for optimized CRISPRi activity
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122239-plasmid-map-sequence-id-292012

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  2. pCAGGS-mTagBFP2-T2A-iCre (v2)

    Plasmid
    #125822
    Purpose
    Monomeric TagBFP2 and iCre recombinase (version 2)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCre
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    125822-plasmid-map-sequence-id-247153

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  12. C1-MPAct-mCherry

    Plasmid
    #155222
    Purpose
    Monitor changes in the density of membrane-proximal F-actin (MPA)
    Depositor
    Tobias Meyer
    Article
    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210
    Insert
    F-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155222-plasmid-map-sequence-id-310131

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  13. dCas12Max-CBE

    Plasmid
    #195337
    Purpose
    vector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized dCas12Max-CBE
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N243R, E336R, D1049A
    Promoter
    CBh, CMV, hU6
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195337-plasmid-map-sequence-id-383131

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  17. pSLIK-Hygro

    Plasmid
    #25737
    Purpose
    3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.
    Depositor
    Iain Fraser
    Article
    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 13, 2010
    Availability
    Academic Institutions and Nonprofits only
    25737-plasmid-map-sequence-id-202030

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  23. N174-MCS

    Plasmid
    #81061
    Purpose
    Lentiviral vector for constitutive gene expression.
    Depositor
    Adam Karpf
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α
    Available since
    Sept. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    81061-plasmid-map-sequence-id-359456

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  34. plex_307-ccdB-EYFP

    Plasmid
    #201146
    Purpose
    pLEX_307 plasmid with EYFP marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201146-plasmid-map-sequence-id-395512

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  37. pMH004_phiC31_neo-HS4-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid
    #179432
    Purpose
    Dual fluorescent reporter construct with double HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    HS4-TRE-cit-HS4-mch (DH)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF alpha, pRSV
    Available since
    May 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    179432-plasmid-map-sequence-id-362555

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  45. gCBEv3

    Plasmid
    #220618
    Purpose
    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv3) driven by EF1a promoter, guide RNAs compatible with SpG driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220618-plasmid-map-sequence-id-436973

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  48. pcDNA3-ER-AKAR4

    Plasmid
    #64733
    Purpose
    Endoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.
    Depositor
    Jin Zhang
    Article
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    AKAR4
    Use
    Tags
    Cerulean, N-terminal 27 amino acids from CYP450, …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64733-plasmid-map-sequence-id-109352

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Showing: 7101 - 7150 of 76300 results