76,351 results
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pLenti-SF3B1-K700E-reporter
Plasmid#200318PurposeA fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.DepositorInsertBSD-2A-EGFPi
UseLentiviralTagsExpressionMammalianMutationPromoterEF1-aAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-mXFPm-IFNGR1(18-489)
Plasmid#192786PurposeExpression of mXFPm-tagged IFNGR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNGR1 (IFNGR1 Human)
UseTagsIg k-chain leader sequence and mXFPmExpressionMammalianMutationmXFPm: tryptophan 66 to phenylalanine, gluatmic a…PromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-3xHA-Puro
Plasmid#207729PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Puro cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAGGS-C-TEV-Twin-Strep
Plasmid#202527PurposeExpress N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cellDepositorTypeEmpty backboneUseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationPromoterCAGAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLAS2w.Phyg-HAVCR1
Plasmid#232669Purposeexpresses HAVCR1 in mammalian cellsDepositorInsertHAVCR1 (HAVCR1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mScarlet-Puro-H2BC11
Plasmid#207761PurposeDonor template for mScarlet-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorInsertH2BC11 Homology Arms flanking a mScarlet-Puro Cassette (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
CMV-mEGFP-CREB
Plasmid#137001PurposemEGFP-CREB donor for G-CREBDepositorInsertCREB1 (Creb1 Rat)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-MKLP1
Plasmid#70154Purposemammalian expression of MKLP1 with an mCherry fusionDepositorInsertMKLP1 (KIF23 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-sh-hSOX9-1
Plasmid#40644DepositorInsertsh-hSOX9-1 (SOX9 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
mPlum-C1
Plasmid#54839PurposeLocalization: C1 Cloning Vector, Excitation: 590, Emission: 649DepositorTypeEmpty backboneUseTagsmPlumExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pC-GoldyTALEN
Plasmid#38143Purposedestination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoterDepositorInsertGoldyTALEN
UseTagsAcV5 and FokI homodimerExpressionMammalianMutationTruncate at N and C terminousPromoterminiCaggsAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RHO-IntC-dCas9C-VPR-polyA
Plasmid#166692PurposeExpress C-terminal part of split dCas9-VPRDepositorInsertIntC-dCas9C-VPR
UseAAV and CRISPRTagsExpressionMammalianMutationPromotershort human rhodopsin promoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA
Plasmid#165450PurposeExpress N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoterDepositorInsertsdCas9N-IntN
3x Opn1mw promoter-targeting sgRNAs
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6 and short human rhodopsin promoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
MBP-TSF-TEVcs-ULK1
Plasmid#171416PurposeFor the purifcation of human ULK1 complexDepositorInsertULK1 (ULK1 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
CCR4-Tango
Plasmid#66241PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertCCR4 (CCR4 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-V5-Puro
Plasmid#207730PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Puro cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pC0090
Plasmid#181954PurposeBeta catenin reporter M50 Super 8x (TCF/LEF binding sites) TOPFlash with Gluc/ClucDepositorInsert7xTOPFlash
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-mCherry-KIX-mCherry
Plasmid#137004PurposemCherry-KIX acceptor for G-CREBDepositorInsertKIX domain (Crebbp Rat)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
peGFP C3-SPG20 8xV
Plasmid#218579PurposeExpresses GFP-tagged human SPG20 bulky hydrophobic amino acid mutant in mammalian cellsDepositorInsertSPG20 (SPART Human)
UseTagseGFPExpressionMammalianMutationchanged Tryptophan 419, Leucine 438, Isoleucine 4…PromoterCMVAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB-rDA3h-IRES-EGFP-CAAX
Plasmid#208697PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h and a membrane-localized EGFP in mammalian cellsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_rDA3h
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mScarlet-EFS-Blast
Plasmid#227260PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Blast cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCR8-Tango
Plasmid#66245PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertCCR8 (CCR8 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP-CREB (S133A)
Plasmid#137002PurposemEGFP-CREB donor for G-CREB with S133A mutationDepositorInsertCREB1 (Creb1 Rat)
UseTagsmEGFPExpressionMammalianMutationChanges Serine 133 into AlaninePromoterCMVAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX_307
Plasmid#41392PurposeConstitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tagDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Gateway destination vectorTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-RUNX3-T2A-PuroR
Plasmid#162349PurposeLentiviral expression of RUNX3 under the control of the TetON promoterDepositorInsertRUNX3 (RUNX3 Human)
UseLentiviralTagsT2A-PuroRExpressionMammalianMutationPromoterTet-ONAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
p7140 pHAGE-P-CMVt-N-HA-GAW-EME1
Plasmid#100150PurposeExpresses EME1DepositorInsertEME1 (EME1 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-C1-CAMKIIalpha-T286A
Plasmid#21220DepositorInsertCAMKIIalpha-T286A (Camk2a Rat)
UseTagsGFPExpressionMammalianMutationT286A (Lab plasmid KS 0003).PromoterAvailable sinceJuly 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
FRB_nSuMMVp
Plasmid#118970PurposeExpresses N-terminus of sunflower mild mosaic virus protease (SuMMVp) fused to the FKBP-rapamycin binding (FRB) domainDepositorInsertsplit nSuMMVp in fusion with FRB
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
FKBP_cSuMMVp
Plasmid#119182PurposeExpresses C-terminus of sunflower mild mosaic virus protease (SuMMVp) fused to the FK-506 binding protein (FKBP)DepositorInsertsplit cSuMMVp in fusion with FKBP
UseTagsHA tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0091
Plasmid#181955PurposeBeta catenin reporter control M51 Super 8x (mutated TCF/LEF binding sites) FOPFlash with Gluc/ClucDepositorInsert7xFOP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-C1-CAMKIIalpha-K42R
Plasmid#21221DepositorInsertCAMKIIalpha-K42R (Camk2a Rat)
UseTagsGFPExpressionMammalianMutationK42R (Lab plasmid KS 0007).PromoterAvailable sinceJuly 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA-DP-1
Plasmid#133705PurposeExpression of HA-tagged TFDP1.DepositorInsertTFDP1 (TFDP1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHM092
Plasmid#225526PurposeEncodes rtTA under AR8 promoter, and constitutive BFPDepositorInsertsrtTA
BFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHM093
Plasmid#225525PurposeEncodes rtTA under IBRE4 promoter, and constitutive BFPDepositorInsertsrtTA
BFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHM039
Plasmid#225523PurposeEncodes Wnt-responsive rtTA and constitutive BFPDepositorInsertsrtTA
BFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Xph15-GCaMP6f
Plasmid#135538PurposeEncodes a specific PSD-95 binder (Xph15) fused to GCaMP6fDepositorInsertXph15
UseTagsGCaMP6fExpressionMammalianMutationPromoterCMVAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Raptor Halo
Plasmid#112754Purposeexpress Raptor-HaloDepositorInsertRaptor-Halo (RPTOR Human)
UseTagsHaloExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
LLP249 pGK-dCas9-DNMT3a V3
Plasmid#100938PurposedCas9 and DNMT WT driven by pGK promoter and Puromycin driven by EF1aDepositorInsertdCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)
UseCRISPR and LentiviralTags3xHA and 3xTy1ExpressionMammalianMutationD10A, H840A for dCas9PromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
