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Mammalian Expression

76,418 results

Showing: 10851 - 10900 of 76418 results

  7. Rlucrarr3-Flash6

    Plasmid
    #74134
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 410 of Arrestin3.
    Depositor
    Louis Luttrell
    Article
    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi
    Insert
    Arrestin3
    Use
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Promoter
    Available since
    March 25, 2016
    Availability
    Academic Institutions and Nonprofits only

  12. U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti

    Plasmid
    #135955
    Purpose
    Neuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3b
    Depositor
    David Liu
    Article
    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586
    Insert
    Prime editor v2 (PE2) N-terminal
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSynapsin
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    135955-plasmid-map-sequence-id-269512

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  19. V1-STING

    Plasmid
    #124263
    Purpose
    N-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
    Depositor
    Eric Schirmer
    Insert
    TMEM173 (STING1 Human)
    Use
    Tags
    Venus1
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124263-plasmid-map-sequence-id-241474

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  25. DRD2-DuET

    Plasmid
    #213228
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    DRD2 (DRD2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213228-plasmid-map-sequence-id-430789

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  26. pcDNA 3.3 HA-hTSPAN12

    Plasmid
    #115784
    Purpose
    Expresses human TSPAN12 with N-terminal HA-tag in mammalian cells. Contains multiple silent mutations that introduce restriction sites.
    Depositor
    Harald Junge
    Article
    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.
    Insert
    TSPAN12 (TSPAN12 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    multiple silent mutations introduce restriction s…
    Promoter
    CMV
    Available since
    Oct. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    115784-plasmid-map-sequence-id-224023

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  28. TFORF2660

    Plasmid
    #142350
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    STAT1 (STAT1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142350-plasmid-map-sequence-id-397203

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  38. GFP Cav2.2 human HAgHA -plus exon 18a - LONG-Cterm in pcDNA3

    Plasmid
    #198451
    Purpose
    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, contains exon 18a- Long form of alternatively spliced C-teminus, in pcDNA3 vector
    Depositor
    Annette Dolphin
    Article
    Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060
    Insert
    Cav2.2 (CACNA1B Human)
    Use
    Tags
    N-terminal GFP, internal double HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    198451-plasmid-map-sequence-id-392120

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  39. GFP Cav2.2 human HAgHA -minus exon 18a -LONG-Cterm in pcDNA3

    Plasmid
    #198914
    Purpose
    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, does not contain exon 18a - Long form of alternatively spliced C-teminus, in pcDNA3 vector
    Depositor
    Annette Dolphin
    Article
    Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060
    Insert
    Cav2.2 (CACNA1B Human)
    Use
    Tags
    N-terminal GFP, internal double HA
    Expression
    Mammalian
    Mutation
    exon 18a deleted, exon 19 starts 3 nucleotides ea…
    Promoter
    CMV
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    198914-plasmid-map-sequence-id-392117

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  46. MELOPS

    Plasmid
    #80151
    Purpose
    Genetically encoded melanosome-localized pH sensor.
    Depositor
    Santiago Di Pietro
    Article
    Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11
    Insert
    OCA2 (OCA2 Human)
    Use
    Tags
    mNectarine in first luminal loop
    Expression
    Mammalian
    Mutation
    nonpathologic polymorphism D257A, pore mutation V…
    Promoter
    CMV
    Available since
    Sept. 20, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 10851 - 10900 of 76418 results