76,321 results

1. pGP-CMV-NES-jRGECO1a

   Plasmid

   #61563

   Purpose

   Red fluorescent calcium sensor protein

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Insert

   NES-jRGECO1a
   

   Use

   Tags

   nuclear export signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. CROPseq-multi-Puro (pRW1441)

   Plasmid

   #216217

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi construct with Puromycin resistance

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1 GFP shRNA

   Plasmid

   #30323

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   GFP shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. ER-GCaMP6f

   Plasmid

   #182548

   Purpose

   Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

   Depositor

   Chris Richards

   Article

   Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

   Insert

   ER localized P450
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBbi-Pur

   Plasmid

   #60523

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: puromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG

   Plasmid

   #222852

   Purpose

   Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-L
   

   Use

   Tags

   IgG1_Fc

   Expression

   Mammalian

   Mutation

   L234A, L235A, P329G

   Promoter

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-BRD4

   Plasmid

   #65378

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Tags

   EmGFP

   Expression

   Mammalian

   Mutation

   R1232W

   Promoter

   CMV

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti sgRNA(MS2)_puro backbone

   Plasmid

   #73795

   Purpose

   3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-Halotag-FRB-EX-nes_pLX304

   Plasmid

   #120913

   Purpose

   Lentiviral vector expressing HA-Halotag-FRB-EX-NES in mammalian cytosol

   Depositor

   Alice Ting

   Article

   Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

   Insert

   HA-Halotag-FRB-EX-NES
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-mCherry-Mango II x 24

    Plasmid

    #127587

    Purpose

    Expresses the mCherry-Mango II x 24 array from a TetON responsive promoter

    Depositor

    David Rueda

    Article

    Cawte et al Nat Commun. 2020 Mar 9;11(1):1283. doi:

    Insert

    mCherry-Mango II x24
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE Tet ON

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. TERT - WT

    Plasmid

    #213827

    Purpose

    Expresses human telomerase reverse transcriptase, the catalytic subunit of telomerase

    Depositor

    Coleman Lindsley

    Article

    Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

    Insert

    human TERT (TERT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-responsive promoter PTight, consisting of sev…

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti6.2_miRFP670

    Plasmid

    #113726

    Purpose

    Expresses the fluorescent protein miRFP670 in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Insert

    miRFP670
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. cytoplasmic NAD+ biosensor

    Plasmid

    #186787

    Purpose

    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasm

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    cytoplasmic NAD+ biosensor
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-C-Myc-DDK-IRES-Puro HNRNPQ

    Plasmid

    #200729

    Purpose

    Lentiviral expression of Flag-Tagged human HNRNPQ

    Depositor

    Claudio Alarcón

    Insert

    HNRNPQ (SYNCRIP Human)
    

    Use

    Lentiviral

    Tags

    Myc-DDK from backbone plasmid

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiCRISPR-E

    Plasmid

    #78852

    Purpose

    Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette

    Depositor

    Phillip Abbosh

    Article

    Sikder et al Mol Cancer Res. 2020 Dec 3. pii: 1541-77

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Cas9-P2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti_PE7-2A-BSD

    Plasmid

    #234073

    Purpose

    For lentiviral expression of PE7 with blasticidin resistance

    Depositor

    Greg Findlay

    Article

    Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

    Insert

    PE7
    

    Use

    CRISPR and Lentiviral

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α core promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. nuclear NAD+ biosensor

    Plasmid

    #186789

    Purpose

    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleus

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    nuclear NAD+ biosensor
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2-Arl13b-mCardinal

    Plasmid

    #194542

    Purpose

    Expresses Arl13b with mCardinal in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiSAM v2 (Puro)

    Plasmid

    #92062

    Purpose

    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Adam Karpf

    Article

    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (sgRNA) and EF1a (dCas9-VP64)

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP-fMCP

    Plasmid

    #238908

    Purpose

    Contains EGFP-fMCP fluorogenic protein

    Depositor

    Jian-Hui Jiang

    Article

    Zhou et al Angew Chem Int Ed Engl. 2025 Apr 10:e202

    Insert

    EGFP-fMCP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. phOct4-EGFP

    Plasmid

    #38776

    Depositor

    Wei Cui

    Article

    Gerrard et al Stem Cells. 2005;23(1):124-33.

    Insert

    Oct-4 promoter (POU5F1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    human OCT4

    Available since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1(+)-ExRai-AKAR2

    Plasmid

    #161753

    Purpose

    Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase A activity in living cells and in vivo.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    ExRai-AKAR2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGP-AAV-syn-jGCaMP8m-WPRE

    Plasmid

    #162375

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. V5-miniTurbo-NES_pCDNA3

    Plasmid

    #107170

    Purpose

    expresses V5-tagged miniTurbo in the mammalian cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mito NAD+ biosensor

    Plasmid

    #186791

    Purpose

    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondria

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    mito NAD+ biosensor
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. plenti.CMV.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #236407

    Purpose

    Expresses cytosolic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV5-Flag-XBP1s

    Plasmid

    #63680

    Purpose

    Mammalian expression of Flag tagged XBP1s

    Depositor

    Lingfang Zeng, Qingbo Xu

    Article

    Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

    Insert

    XBP1 (XBP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    spliced

    Promoter

    CMV

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti6.3/V5-DEST-GFP

    Plasmid

    #40125

    Depositor

    Lynda Chin

    Article

    Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p53-TRE-mStrawberry reporter

    Plasmid

    #158684

    Purpose

    This plasmid is a p53 pathway reporter. It has two copies of wild-type p53 binding sites promoter driving the expression of mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. c-myc-PT3EF1a

    Plasmid

    #92046

    Purpose

    Expresses c-Myc in mammalian cells

    Depositor

    Xin Chen

    Article

    Liu et al Hepatology. 2017 Mar 30. doi: 10.1002/he

    Insert

    C-MYC (MYC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    July 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. shp53 pLKO.1 puro

    Plasmid

    #19119

    Purpose

    3rd gen lentiviral vector for knocking down p53 gene expression

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    small hairpin RNA against tumor protein p53 (TP53 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCAG-tTA

    Plasmid

    #104102

    Purpose

    Expresses tTA2 under the CAG promoter in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

    Insert

    tTA2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. p7056 pHAGE-P-CMVt-N-HA-GAW-CPT1A

    Plasmid

    #100146

    Purpose

    Expresses CPT1A

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    CPT1A (CPT1A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHAGE2_EF1a_TetOn_IRES_mCherry

    Plasmid

    #204155

    Purpose

    Lentivirus backbone expressing rtTA linked to mCherry

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    Tet-On 3G
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMXs-c-Myc

    Plasmid

    #13375

    Purpose

    Retroviral expression of mouse c-Myc

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    myelocytomatosis oncogene (Myc Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSBbi G418R mbIL21

    Plasmid

    #194329

    Purpose

    Consitiutive mammalian expression of membrane bound human IL-21

    Depositor

    Alexander McLellan

    Article

    Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

    Insert

    mbIL-21 (IL21 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-Ef1a-fDIO hChR2(H134R)-EYFP

    Plasmid

    #55639

    Purpose

    Flp-Dependent ChR2-EYFP

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde

    Insert

    hChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA 3.1(Hygro) myc-hPolQ-Flag

    Plasmid

    #73132

    Purpose

    To express in mammalian cells human PolQ

    Depositor

    Agnel Sfeir

    Article

    Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

    Insert

    Polymerase theta (POLQ Human)
    

    Use

    Tags

    Flag and myc

    Expression

    Mammalian

    Mutation

    Promoter

    cmv promoter

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3-ExRai-CKAR2

    Plasmid

    #236096

    Purpose

    Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

    Depositor

    Jin Zhang

    Article

    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

    Insert

    ExRai-CKAR2
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid

    #118154

    Purpose

    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-P2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    EF1a core and U6

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge