79,061 results

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pLJM1-Tmem192-mRFP-3xHA

Plasmid

#134631

Purpose

bait for affinity-isolation of lysosomes

Depositor

Roberto Zoncu

Article

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

TMEM192 (TMEM192 Human)

Use

Lentiviral

Tags

3xHA, TEV, and mRFP

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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mEmerald-Calreticulin-N-16

Plasmid

#54023

Purpose

Localization: Endoplasmic Reticulum, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Calreticulin (CALR Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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psiCHECK-2-XNS

Plasmid

#196655

Purpose

Promega's psiCHECK-2 Dual-Luciferase Reporter vector modified to allow the insertion of the target in opposite directions

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Type

Empty backbone

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUAS-NanoLuc

Plasmid

#87696

Purpose

Gal4VP16 driven expression of NanoLuc

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

NanoLuc

Tags

nuclear localization signal

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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LentiSAMPHv2

Plasmid

#167934

Purpose

All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-BICD1

Plasmid

#49487

Purpose

expresses EGFP-BICD1 in mammalian cells

Depositor

Marci Scidmore

Article

Moorhead et al Infect Immun. 2007 Feb;75(2):781-91. Epu

Insert

BICD1 (BICD1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

** see notes

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
Tet-inducible Luciferase reporter

Plasmid

#64127

Purpose

Photoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

luciferase

Use

Luciferase

Expression

Mammalian

Promoter

TetOx13-CMV

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCW empty vector

Plasmid

#184708

Purpose

Lentiviral empty vector for doxycycline-inducible expression

Depositor

Alessia Ciarrocchi, Gloria Manzotti

Article

Gugnoni et al J Exp Clin Cancer Res. 2022 Mar 25;41(1)

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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TDP-43 WT GFP

Plasmid

#237542

Purpose

Expression of GFP-tag fused to wild-type TDP-43

Depositor

Tatyana Shelkovnikova

Article

Matsukawa et al RNA Biol. 2021 Jan 11:1-9. doi: 10.1080/

Insert

GFP-tagged TDP-43 WT (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

wild-type TDP-43

Promoter

CMV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pSP2-48-merTetO-EFS-BLaR

Plasmid

#118712

Purpose

Contains an optimized 48-mer TetO repeat for imaging of desired loci

Depositor

Huimin Zhao

Article

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Inserts

Blasticidin resistance gene
EFS-NS promoter

Expression

Mammalian

Promoter

EFS-NS

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-Smad3

Plasmid

#172098

Purpose

Expresses fluorescently tagged human Smad3 in mammalian cells.

Depositor

Lea Goentoro

Article

Frick et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Insert

Smad 3 (Smad3 Mouse)

Tags

mNeonGreen

Expression

Mammalian

Mutation

C49S mutation in mCerulean

Promoter

CMV

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Human SERCA2b (pcDNA3.1+)

Plasmid

#75188

Purpose

Mammalian expression of ATP2A2

Depositor

Jonathan Lytton, David MacLennan

Article

Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

Insert

ATP2A2 (ATP2A2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pABpuro-BluF

Plasmid

#46824

Purpose

luciferase reporter for BmalI promoter

Depositor

Steven Brown

Article

Brown et al PLoS Biol. 2005 Oct;3(10):e338. Epub 200

Insert

Bmal1 (Bmal1 Mouse)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Bmal1

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pXJ219-pAAV-PB3'IR-U6-gRNA-EF1a-mtagBFP2-KASH-PB5'IR

Plasmid

#210525

Purpose

AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

Depositor

Xin Jin

Article

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

mtagBFP-KASH
U6-gRNA

Use

AAV

Expression

Mammalian

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Pepper-fused sgRNA

Plasmid

#217520

Purpose

Expresses Pepper-fused sgRNA in mammalian cells with U6 promoter

Depositor

Xing Li

Article

Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

Insert

Pepper-fused sgRNA for spCas9

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMVHA E2F1

Plasmid

#24225

Purpose

Mammalian expression of human E2F1 with HA tag

Depositor

Kristian Helin

Article

Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

Insert

E2F1 (E2F1 Human)

Tags

HA

Expression

Mammalian

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)

Plasmid

#140659

Purpose

OsTIR1(F74G)-P2A-mAID-EGFP-Nluc

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74G) mAID-EGFP-Nluc TOL2

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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5HT6-mCherry-G-GECO1.0

Plasmid

#47500

Purpose

Fluorescent reporter for Ca2+ inside primary cilia with mCherry reference

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

G-GECO1.0

Tags

5HT6 and mCherry

Expression

Mammalian

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pUltra-Hot-PC

Plasmid

#184466

Purpose

Lentiviral vector for bi-cistronic expression of mCherry and PC (seperated by P2A)

Depositor

Gerardo Ferbeyre

Article

Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

Insert

Pyruvate Carboxylase (PC Human)

Use

Lentiviral

Expression

Mammalian

Promoter

UbC

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-CD36-C-10

Plasmid

#55011

Purpose

Localization: Membrane, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

CD36 (CD36 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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