78,292 results

1. TFORF3536

   Plasmid

   #145012

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   HOXD4 (HOXD4 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2 GFP-GSK3-MAPK

   Plasmid

   #29689

   Purpose

   GFP reporter protein fused to three GSK3 phosphorylation sites

   Depositor

   Edward De Robertis

   Article

   Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

   Insert

   GFP glycogen synthase kinase 3 recognition site primed by MAPK
   

   Tags

   Flag and Flag-EGFP

   Expression

   Mammalian

   Mutation

   recognition sites only, and E3 ligase site

   Promoter

   SP6

   Available Since

   July 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti6.3-enCjCas9-BlastR

   Plasmid

   #220978

   Purpose

   Expresses human codon-optimized enCjCas9

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   enCjCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF0427

   Plasmid

   #142620

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PBX1 (PBX1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDisplay-GRAB_OT1.0

   Plasmid

   #185384

   Purpose

   Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   GPCR activation based oxytocin sensor GRAB_OT1.0
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-gp78-FLAG

   Plasmid

   #62370

   Purpose

   Express C-terminally FLAG tagged gp78

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   gp78 (AMFR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   *

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-MRLC1 T18D, S19D

   Plasmid

   #35682

   Depositor

   Tom Egelhoff

   Article

   Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

   Insert

   MRLC1 (MYL9 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   T18D and S19D

   Promoter

   CMV

   Available Since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pMito-miRFP703

   Plasmid

   #80000

   Purpose

   Mitochondria labeling with near-infrared fluorescent protein miRFP703

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

   Insert

   cytochrome C oxidase subunit VIII (COX8A Human)
   

   Tags

   miRFP703

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-MRLC T18A,S19A

   Plasmid

   #35681

   Depositor

   Tom Egelhoff

   Article

   Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

   Insert

   MRLC1 (MYL9 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   T18A and S19A

   Promoter

   CMV

   Available Since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pCS2 GFP-GSK3mut-MAPK

    Plasmid

    #29690

    Purpose

    GFP reporter protein fused to three mutated GSK3 phosphorylation sites

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    GFP glycogen synthase kinase 3 recognition site mutated primed by MAPK
    

    Tags

    Flag and Flag-EGFP

    Expression

    Mammalian

    Mutation

    recognition sites only, and E3 ligase site, GSK3 …

    Promoter

    SP6

    Available Since

    Jan. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. tdTomato-Mito-7

    Plasmid

    #58115

    Purpose

    Localization: Mitochondria, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Article

    Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

    Insert

    Mito (COX8A Human)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

12. TFORF0431

    Plasmid

    #142622

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    AIRE (AIRE Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX404 Campylobacter jejuni Cas9

    Plasmid

    #68338

    Purpose

    Campylobacter jejuni Cas9

    Depositor

    Feng Zhang

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    HA-NLS and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCD5-H3TX12-CO-GCN4-TEV-sfGFP-TS

    Plasmid

    #158739

    Purpose

    Expresses influenza A virus trimeric hemagglutinin A/H3/TX/12 fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

    Insert

    Hemagglutinin A/TX/50/12 H3
    

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Mutation

    G186V S219F

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF3119

    Plasmid

    #144595

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    DDIT3 (DDIT3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. psd44-G12QL

    Plasmid

    #46825

    Purpose

    expression of mutant G protein alpha 12 in mammalian cells

    Depositor

    Agnese Mariotti

    Insert

    GNA12 (GNA12 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Q231L (the mutation reduces GTPase activity resul…

    Promoter

    PGK

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCD5-H3CH13-CO-GCN4-TEV-sfGFP-TS

    Plasmid

    #158740

    Purpose

    Expresses influenza A virus trimeric hemagglutinin A/H3/CH/13 fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

    Insert

    Hemagglutinin A/MI/45/15 H1N1
    

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Mutation

    AA61-469

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCIS.MTRIA-NPFF

    Plasmid

    #184612

    Purpose

    For expression of a fluorescent sensor for neuropeptide FF in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-NPFF
    

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3.1-HsPcdh21-mNeonGreen

    Plasmid

    #210819

    Purpose

    Mammalian overexpression vector for C-terminally mNeonGreen tagged human Pchd21 (WT)

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    Protocadherin 21 (CDHR1 Human)
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTriEx-mVenus-Zdk1

    Plasmid

    #81047

    Purpose

    mammalian expression of Zdk1

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk1
    

    Tags

    6x His tag, FLAG, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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