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Plasmid#121054PurposeGFP^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.DepositorInsertGFP^SEC^AID*::3xFlag
UseCRISPR and Cre/LoxTags3xFLAG, C. elegans codon optimized GFP, and auxin…ExpressionWormMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSM03
Plasmid#139043PurposeMosSCI SapTrap assembly: Cassette 3 for 4-cassette systemDepositorInsertdendra2 (includes STOP codon)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSDH61
Plasmid#139033PurposeMosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette systemDepositorInsertepdz (no STOP codon)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
L4053
Plasmid#1631DepositorTypeEmpty backboneUseTagsIVSA, ATG-SecSig, pes10ExpressionWormMutationPromoterAvailable sinceMarch 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pBCN27-Pmyo-2::GFP::unc-54_3'UTR
Plasmid#26347DepositorInsertPmyo-2::GFP::unc-54_3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceDec. 6, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAB16 (pmyo-3::QuasAr)
Plasmid#130272PurposeExpresses the voltage sensor QuasAr in BWMs of C. elegans.DepositorInsertQuasAr
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1955
Plasmid#84830PurposeMinimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic intronsDepositorInsertC. elegans codon optimized tdTomato
UseTagsExpressionBacterial and WormMutationPromoterPeft-3Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGC185
Plasmid#19624DepositorInsertPrpl-28<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<4xNLS-tdimer2(12)::let-858(3’)
UseFlp/frtTagsExpressionWormMutationPromoterAvailable sinceFeb. 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJH127
Plasmid#162483PurposeSEC plasmid containing LG1 homology arms and myo-2p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.DepositorInsertmyo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
UseCRISPRTagsmyo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTRExpressionWormMutationPromoterAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
BSP611
Plasmid#122256Purposeexpression vector for mtagRFP-T under EFT3 promoterDepositorInsertmtagRFP-T
UseTagsExpressionWormMutationPromoterEFT3Available sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMLS485
Plasmid#188890PurposeWorm Cas9 expression vector, Peft-3 promoterDepositorInsertPeft-3::Cas9
UseTagsExpressionWormMutationC. elegans codom optimization and intron additionPromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ352
Plasmid#30539DepositorInsertttTi4348 region
UseTagsExpressionWormMutationPromoterAvailable sinceJune 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCFJ178 - cxTi10882_MCS
Plasmid#19331DepositorArticleInsertcxTi10882 targetting region
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDD04neo pmyo2::gfp
Plasmid#26393DepositorTypeEmpty backboneUseTagsExpressionBacterial and WormMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]
Plasmid#159798PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGC257
Plasmid#19660DepositorInsertUseFlp/frtTags4xNLSExpressionWormMutationPromoterAvailable sinceFeb. 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pGC381
Plasmid#19663DepositorInsert<*-CFP::let-858(3’)
UseFlp/frtTagsExpressionWormMutationPromoterAvailable sinceFeb. 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMZ9
Plasmid#198786PurposeDrives specific expression in the AWC (ON) neuronsDepositorInsertPsrt-28-GAL4-SK(DBD)-VP64
UseTagsExpressionWormMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCC14
Plasmid#198781PurposeDrives specific expression in the ASJ neuronsDepositorInsertPtrx-1-GAL4-SK(DBD)-VP64
UseTagsExpressionWormMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMZ10
Plasmid#198787PurposeDrives specific expression in the AWC (OFF) neuronsDepositorInsertPsrt-47-GAL4-SK(DBD)-VP64
UseTagsExpressionWormMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWP005
Plasmid#198785PurposeDrives specific expression in the AWB neuronsDepositorInsertPstr-1-GAL4-SK(DBD)-VP64
UseTagsExpressionWormMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL065
Plasmid#198784PurposeDrives specific expression in the AWA neuronsDepositorInsertPodr-10-GAL4-SK(DBD)-VP64
UseTagsExpressionWormMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWP061
Plasmid#198775PurposeDrives specific expression in the ASE neuronsDepositorInsertPgcy-5-GAL4-SK(DBD)-VP64
UseTagsExpressionWormMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEY51
Plasmid#191054Purposemec-3 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertmec-3 (mec-3 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ccas9LacZ
Plasmid#113083PurposeEnhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.DepositorTypeEmpty backboneUseCRISPRTagsExpressionWormMutationPromoterAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)
Plasmid#154840PurposeInsertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.DepositorInserthomology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
UseCRISPR and Cre/LoxTagsExpressionWormMutationHYGR∆ encodes aa 1-226PromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCZGY1534 tomm-20N::miniSOG in pENTR[1-2]
Plasmid#66753PurposeminiSOG fused to N terminal 55 aa of C. elegans tomm-20 for targeting to the mitochondriaDepositorInsertminiSOG
UseTagstomm-20 (N terminal 55 aa)ExpressionWormMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEY26
Plasmid#191031Purposecho-1(3.3-2.6k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorInsertcho-1(3.3-2.6k) (cho-1 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY39
Plasmid#191043Purposeacr-2(2.1k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertacr-2(2.1k) (acr-2 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJR81
Plasmid#75029PurposetagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInserttagBFP2^SEC^3xFlag
UseCRISPRTags3xFLAG and C. elegans codon optimized tagBFP2ExpressionWormMutationPromoterAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEY52
Plasmid#191055Purposeodr-1 fluorescent neural reporter driving nuclear mNeptune2.5 and mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertodr-1 (odr-1 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2475 - pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
Plasmid#159813PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
TC476
Plasmid#122629PurposePunc-54::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1DepositorInsertrtetR-QFAD::P2A::mKate::T2A::tetR-pie1
UseTagsExpressionWormMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
PU6::klp-12_sgRNA
Plasmid#46170Purposeklp-12 targeting sgRNADepositorInsertklp-12 targeting sgRNA (klp-12 Synthetic)
UseCRISPRTagsExpressionWormMutationPromoterC. elegans U6 snRNA pol III promoterAvailable sinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1655
Plasmid#51481PurposeminiMos transposable element with HygroR, R4-R3 Gateway cloningDepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRB1080
Plasmid#71309PurposeExpresses VQR Cas9 variant with eef-1A.1 (eft-3) promoterDepositorInsertVQR Cas9 variant
UseCRISPRTagsExpressionWormMutationPromotereef-1A.1 (eft-3)Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2334 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)
Plasmid#159850PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDJ12 - pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]
Plasmid#159799PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Pvha6-AgDD
Plasmid#80626Purposecreate protein aggregates in C. elegansDepositorInsertNES-L106P-sfGFP (FKBP1A Human)
UseTagsMLALKLAGLDIExpressionWormMutationPromoterAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only