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8,173 results

Showing: 1 - 50 of 8173 results

  1. pV1524

    Plasmid
    #111431
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)
    Depositor
    Gerald Fink
    Article
    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111431-plasmid-map-sequence-id-292191

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  4. pML104

    Plasmid
    #67638
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    pSNR52 and pTDH3
    Available Since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67638-plasmid-map-sequence-id-322882

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  9. pFA6a-link-ymTurquoise2-Kan

    Plasmid
    #224092
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with Kan selection
    Depositor
    Bas Teusink
    Insert
    ymTurquoise2
    Tags
    ymTurquoise2
    Expression
    Yeast
    Available Since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224092-plasmid-map-sequence-id-453797

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  16. pYD1

    Plasmid
    #73447
    Purpose
    expression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cells
    Depositor
    Dane Wittrup
    Article
    Kieke et al Protein Eng. 1997 Nov;10(11):1303-10.
    Type
    Empty backbone
    Tags
    6xHis, AGA2, T7 peptide, V5 tag, and Xpress tag
    Expression
    Yeast
    Promoter
    GAL1
    Available Since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    73447-plasmid-map-sequence-id-364550

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  19. pFA6a-HaloTag-KanMX6

    Plasmid
    #87029
    Purpose
    Tag S. pombe genes in C terminal with HaloTag
    Depositor
    Julien Berro
    Insert
    HaloTag
    Expression
    Bacterial and Yeast
    Available Since
    Feb. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87029-plasmid-map-sequence-id-173001

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  21. pDEST-N2H-C1

    Plasmid
    #125549
    Purpose
    Gateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment1
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment1 attached to c-terminus of Gatew…
    Expression
    Mammalian and Yeast
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125549-plasmid-map-sequence-id-254837

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  22. pDEST-N2H-C2

    Plasmid
    #125559
    Purpose
    Gateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment2
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment2 attached to c-terminus of Gatew…
    Expression
    Mammalian and Yeast
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125559-plasmid-map-sequence-id-254701

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  23. pDEST-N2H-N2

    Plasmid
    #125548
    Purpose
    Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment2
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment2 attached to n-terminus of Gatew…
    Expression
    Mammalian and Yeast
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125548-plasmid-map-sequence-id-254836

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  24. pLSB-NAT

    Plasmid
    #166698
    Purpose
    Combined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA promoter
    Available Since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166698-plasmid-map-sequence-id-329407

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  25. pJYDN

    Plasmid
    #162450
    Purpose
    Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    Aga2p, HA, myc tags, and eUnaG2 fluorescent prote…
    Expression
    Yeast
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162450-plasmid-map-sequence-id-321442

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  26. pML107

    Plasmid
    #67639
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    pSNR52 and pTDH3 (aka GAP promoter)
    Available Since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67639-plasmid-map-sequence-id-220324

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  31. LHZ1493

    Plasmid
    #222102
    Purpose
    Cloning backbone for sgRNA in Kluyveromyces marxianus. Expresses highly specific SlugCas9.
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    222102-plasmid-map-sequence-id-441240

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  32. pFA6a-CLIP-KanMX6

    Plasmid
    #87025
    Purpose
    Tag S. pombe genes in C terminal with CLIP
    Depositor
    Julien Berro
    Insert
    CLIP
    Expression
    Bacterial and Yeast
    Available Since
    Feb. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    87025-plasmid-map-sequence-id-172996

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  37. pRS416-yZ3EV-Z3pr (RB3578)

    Plasmid
    #69099
    Purpose
    URA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr
    Depositor
    David Botstein
    Inserts
    yZ3EV cassette
    Z3 promoter
    Expression
    Yeast
    Available Since
    Sept. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    69099-plasmid-map-sequence-id-122355

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  43. pNA0304

    Plasmid
    #165612
    Purpose
    Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    NotI site replaced the CAN1 spacer in parent vect…
    Promoter
    SNR52
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165612-plasmid-map-sequence-id-331070

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  49. GFP-ATG8(416)/GFP-AUT7(416)

    Plasmid
    #160389
    Purpose
    Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter , NAT selection
    Depositor
    John McCusker
    Insert
    autophagy-related 8 (ATG8 Budding Yeast)
    Use
    Cen/ars
    Expression
    Yeast
    Promoter
    Endogenous ATG8 promoter
    Available Since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    160389-plasmid-map-sequence-id-314524

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  50. pFA6a-link-ymScarletI3-URA3

    Plasmid
    #224075
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with URA3 selection
    Depositor
    Bas Teusink
    Insert
    ymScarletI3
    Tags
    ymScarletI3
    Expression
    Yeast
    Available Since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224075-plasmid-map-sequence-id-460510

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Showing: 1 - 50 of 8173 results