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8,265 results

Showing: 1 - 50 of 8265 results

  4. pPMVAd3

    Plasmid
    #98298
    Purpose
    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.
    Depositor
    Claudia Vickers
    Article
    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.
    Insert
    PRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A
    Expression
    Yeast
    Available Since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98298-plasmid-map-sequence-id-190181

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  5. pML107

    Plasmid
    #67639
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    pSNR52 and pTDH3 (aka GAP promoter)
    Available Since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67639-plasmid-map-sequence-id-220324

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  8. ZJOM68

    Plasmid
    #133657
    Purpose
    Integration of TGL3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast lipid droplet.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-TGL3-mCherry (TGL3 Budding Yeast)
    Tags
    mCherry
    Expression
    Yeast
    Available Since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133657-plasmid-map-sequence-id-268966

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  13. pPMVAu5 (R)

    Plasmid
    #98296
    Purpose
    Enhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.
    Depositor
    Claudia Vickers
    Article
    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.
    Insert
    PSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1
    Expression
    Yeast
    Available Since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    98296-plasmid-map-sequence-id-201300

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  17. pTSK450

    Plasmid
    #34552
    Depositor
    James McNally
    Insert
    MS2-CP-NLS
    Tags
    mCherry
    Expression
    Yeast
    Promoter
    Met25
    Available Since
    Jan. 19, 2012
    Availability
    Academic Institutions and Nonprofits only
    34552-plasmid-map-sequence-id-41124

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  24. Linked Free NES (M)

    Plasmid
    #182436
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Insert
    FPPS(M)-NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182436-plasmid-map-sequence-id-360120

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  31. pJRoC30_Th3

    Plasmid
    #188064
    Purpose
    PROSS-designed high-redox potential laccase from Trametes hirsuta
    Depositor
    Sarel Fleishman
    Article
    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173
    Insert
    PROSS-designed high-redox potential laccase from Trametes hirsuta
    Tags
    S. cerevisiae evolved α-factor prepro-leader sign…
    Expression
    Yeast
    Mutation
    20 mutations, described in publication
    Available Since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    188064-plasmid-map-sequence-id-370132

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  39. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid
    #104909
    Purpose
    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    June 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    104909-plasmid-map-sequence-id-206071

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  42. BB3cH_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104907
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Jan. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    104907-plasmid-map-sequence-id-206042

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  44. pFTK093

    Plasmid
    #171365
    Purpose
    Vector part (LVL1) from the Fungal Modular Cloning ToolKit.
    Depositor
    Arnold Driessen
    Article
    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021
    Insert
    sgRNA transcription unit (MoClo lvl1 unit), P-gpdA-HH-sgRNA-HDV-Ttrpc, replacable LacZ gene
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Mutation
    n/a
    Available Since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    171365-plasmid-map-sequence-id-352204

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  46. pUCC001

    Plasmid
    #124451
    Purpose
    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets
    Depositor
    John Morrissey
    Article
    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97
    Insert
    HH-BsaI
    Expression
    Yeast
    Mutation
    gRNA expression cassette from pUDP002 modified to…
    Promoter
    TDH3 promoter from S.cerevisiae (already present …
    Available Since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    124451-plasmid-map-sequence-id-257763

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  47. pWS172

    Plasmid
    #90519
    Purpose
    Cas9 gap repair vector - HIS3
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Feb. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    90519-plasmid-map-sequence-id-287208

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  48. pML104

    Plasmid
    #67638
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    pSNR52 and pTDH3
    Available Since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67638-plasmid-map-sequence-id-322882

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Showing: 1 - 50 of 8265 results