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Plasmid#126717Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
UseTagsExpressionYeastMutationPromoterGAPAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMVS142_pACT1_mCherry_Zif268_EBD_MCS_KAN
Plasmid#99049PurposeTranscription Factor chassis for activation domains. mCherry, Zif268 DNA binding domain, estrogen response domainDepositorInsertsUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDP0437
Plasmid#221127PurposePlasmid for generating integration cassette to C-terminally tag gene of interest with pHluorin::HPHMXDepositorInsertpHluorin
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG10
Plasmid#164702PurposeyEVenus under the control of GALL promoterDepositorInsertGALLpr - yEVenus - CLN2 PEST - ADH1t
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGAL-PDE1
Plasmid#160388PurposeOverxpression of PDE1 in yeastDepositorInsertPDE1
UseTagsExpressionYeastMutationPromoterGALAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pV1382
Plasmid#111436PurposeS. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatRDepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pV1090
Plasmid#111427PurposegRNA entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at RPS1 (Part of Duet system)DepositorInsertsgRNA-BsmBI stuffer
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
GAL FLAG103QDProGFPp303
Plasmid#15587DepositorInserthtt 103Q Delta Pro (HTT Human)
UseTagsFLAG and GFPExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
Epac-SH225
Plasmid#191449Purposeexpression of cAMP sensor in yeastDepositorInsertmT2Del-EPACdDEPCD-tdTomato
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
ZJOM82
Plasmid#133648PurposeIntegration of NAB2-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast nucleus.DepositorInsertp1k-NAB2-mCherry (NAB2 Budding Yeast)
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pAG425GAL-ccdB
Plasmid#14153DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseGateway destinationTagsExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-PAmCherry-KanMX6
Plasmid#87026PurposeTag S. pombe genes in C terminal with PAmCherryDepositorInsertPAmCherry
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBHM2329
Plasmid#171686PurposepRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCRDepositorInsertPCnU6-sgRNA_scaffold
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterC. neoformans U6Available sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTH761-CEN-mCherry_v4
Plasmid#40599DepositorInsertmCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences
UseTagsExpressionYeastMutationmCherry ORF partially codon optimised for yeast e…PromoterGPD (TDH3)Available sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAV125
Plasmid#63191PurposepRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCas3
Plasmid#229538PurposepMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.DepositorInsertCas3
UseCRISPRTagsExpressionBacterial and YeastMutationcodon optimized for S.cerevisiaePromoterAvailable sinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGAN230
Plasmid#131122Purposereverse yTRAP, RNQ1-aTF reporting on tetR-mKate2DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterSUP35Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAR-Ec318
Plasmid#130871PurposeA nuclear plasmid encoding the WT TP-DNAP1 for OrthoRepDepositorInsertTP-DNAP1
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYD1-mSA
Plasmid#39865DepositorInsertmonomeric streptavidin
UseTagsAga2, FLAG, and HAExpressionYeastMutationPromoterAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGTy1mhis3-AI or pGTy1his3-AI
Plasmid#62228PurposeA Ty1 element tagged with the retrotransposition indicator gene his3-AI is fused to the GAL1 promoter on a multicopy URA3-based shuttle plasmid.DepositorInsertTy1H3 retrotransposon
UseTagsExpressionYeastMutationPromoterGAL1Available sinceFeb. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pESC-LEU (V5 and HA)
Plasmid#222486PurposeCo-expression of two genes in yeast with V5 and HA tags and LEU2 selectionDepositorTypeEmpty backboneUseTagsHA and V5ExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEmpty_Control
Plasmid#162607PurposeEmpty control for Cas9 experiments and to clone new guide RNAs for paperDepositorInsertTef1-Cas9 with RPL25 Intron
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGAL-GFP kan
Plasmid#122060Purposegalactose inducible expression of GFP in yeast. marked with URA3DepositorInsertgreen fluorescent protein
UseTagsExpressionYeastMutationPromoterGAL1Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal Q331K YFP
Plasmid#27450DepositorInsertTDP-43 (TARDBP Human)
UseTagsYFPExpressionYeastMutationGlutamine 331 mutated to Lysine (Q331K)PromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKate2-CaURA3
Plasmid#44878PurposeYeast tagging vector to create gene fusion with yeast optimized mKate2 with CaUra3 selectionDepositorInsertyomKate2
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSK221
Plasmid#129249Purposereverse yTRAP, tetR-responsive Neon reporterDepositorInsertpADH1(tetO2)-mNeonGreen
UseTagsExpressionYeastMutationPromoterADH1Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pILGFP91
Plasmid#83548PurposeUsed to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.DepositorInsertPGK1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterPGK1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCYC2
Plasmid#43970DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterCYC1Available sinceApril 9, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
ZJOM64
Plasmid#133655PurposeIntegration of COX4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.DepositorInsertp1k-COX4-DuDre (COX4 Budding Yeast)
UseTagsDuDreExpressionYeastMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRG207
Plasmid#64530PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG416-Gal-PR50
Plasmid#84901Purposeinduce expression of dipeptide repeat PR in yeastDepositorInsertPR50 (C9orf72 Synthetic)
UseTagsFLAG and MycExpressionYeastMutationPromoterGAL1Available sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV0765
Plasmid#133501PurposeExpression of mCherry targeted to the nucleus with one NLS signalsDepositorInsertpLys3(BstZ17I)-p(tdh1)-NLSSV40-mCherry-terminator(ScADH1)-kanMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymScarletI-URA3
Plasmid#168055PurposeReplaces Addgene plasmid 118457. Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymScarletI
UseTagsymScarletIExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRG705_lexO-AsiSI_LexA-TF_LEU2MX
Plasmid#154817PurposeInducible expression of AsiSI endonuclease in S. cerevisiaeDepositorInsertsAsiSI endonuclease
LexA-ER-B112
UseTagsFLAG and NLSExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBHM2641
Plasmid#229525PurposeCarries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
UseCRISPRTagst2a-mNeonGreen-AID*ExpressionBacterial and YeastMutationF74GPromoterTEF1Available sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2642
Plasmid#229526PurposeCarries OsTIR1-t2a-mNG-mIAA7_opt, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
UseTagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2644
Plasmid#229528PurposeCarries AID* tag, empty sgRNA, and NATR markerDepositorInsertAID* - empty sgRNA - NATR
UseTags2xFLAGExpressionBacterial and YeastMutationPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2645
Plasmid#229529PurposeCarries mIAA7_opt tag, empty sgRNA, and NATR markerDepositorInsertmIAA7_opt - empty sgRNA - NATR
UseCRISPRTags2xFLAGExpressionBacterial and YeastMutationPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2646
Plasmid#229530PurposeCarries mAID_opt tag, empty sgRNA, and NATR markerDepositorInsertmAID_opt - empty sgRNA - NATR
UseTags2xFLAGExpressionBacterial and YeastMutationPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only