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8,173 results

Showing: 521 - 560 of 8173 results

  7. pDS240

    Plasmid
    #34966
    Purpose
    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity epitope).
    Depositor
    Michael Glotzer
    Article
    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm
    Insert
    Mid2(SS/TM)-GFP-LOVpep
    Expression
    Yeast
    Mutation
    K533∆, high-affinity epitope
    Promoter
    GAL1
    Available Since
    March 21, 2012
    Availability
    Academic Institutions and Nonprofits only
    34966-plasmid-map-sequence-id-42500

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  16. p2B-58

    Plasmid
    #177928
    Purpose
    Expresses yeGFP under control of doxycycline-responsive TET4 promoter (contains four rtTA binding sites)
    Depositor
    Vadim Gladyshev
    Article
    Barre et al (unpublished)
    Insert
    EGFP
    Expression
    Bacterial and Yeast
    Promoter
    TET4 (contains four rtTA binding sites)
    Available Since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177928-plasmid-map-sequence-id-349996

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  20. pGR475

    Plasmid
    #213784
    Purpose
    Integrates at the CAN1 locus the WT TP-DNAP1 and the Hygromycin resistance marker
    Depositor
    Chang Liu
    Insert
    WT TP-DNAP1 (recoded)
    Use
    Synthetic Biology
    Expression
    Yeast
    Available Since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213784-plasmid-map-sequence-id-424627

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  24. pCASB

    Plasmid
    #190175
    Purpose
    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence
    Depositor
    Jessica Tyler
    Article
    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:
    Insert
    sgRNA cloning site
    Expression
    Bacterial and Yeast
    Mutation
    Added 5'-agagacc-3' upstream and 5'…
    Available Since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    190175-plasmid-map-sequence-id-372326

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  25. p405ADH1

    Plasmid
    #15966
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    p405ADH1 (ADH1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    ADH1 promoter is between SacI and XbaI restrictio…
    Available Since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15966-plasmid-map-sequence-id-8451

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  28. ZJOM135

    Plasmid
    #133659
    Purpose
    Integration of SEC63-2mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-SEC63-2mTagBFP2 (SEC63 Budding Yeast)
    Tags
    mTagBFP2
    Expression
    Yeast
    Available Since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    133659-plasmid-map-sequence-id-265905

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  31. pE6n

    Plasmid
    #17460
    Purpose
    modified pENTR vector for N-terminal EYFP tag fusion with your gene of interest
    Depositor
    Giovanna Benvenuto
    Article
    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.
    Type
    Empty backbone
    Use
    Modified gateway entry vector
    Tags
    EYFP
    Expression
    Bacterial, Insect, Mammalia…
    Available Since
    March 21, 2008
    Availability
    Academic Institutions and Nonprofits only
    17460-plasmid-map-sequence-id-8628

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  32. pEVA1

    Plasmid
    #58953
    Purpose
    Contains a synthetic DNA sequence, VAR1u, that specifies the Var1 protein. The Cox4 targeting signal is fused to the N-terminus to direct import of Var1. Complements var1 mutations in mtDNA.
    Depositor
    Thomas Fox, Thomas Mason
    Article
    Sanchirico et al EMBO J. 1998 Oct 1;17(19):5796-804.
    Insert
    VAR1u, VAR1 recoded for expression from the nucleus
    Use
    Yeast ecoli shuttle vector
    Tags
    preCox4 mitochondrial matrix targeting signal
    Expression
    Yeast
    Mutation
    Synthetic gene in standard genetic code
    Promoter
    ADH1
    Available Since
    Aug. 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  33. p404GALS

    Plasmid
    #17435
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    GALS promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    GALS promoter is between SacI and XbaI restrictio…
    Available Since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17435-plasmid-map-sequence-id-8609

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  34. yeast_DualReporter

    Plasmid
    #127546
    Purpose
    A dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).
    Depositor
    Aviv Regev
    Article
    de Boer et al Nat Biotechnol. 2020 Jan;38(1):56-65. do
    Inserts
    bla
    mCherry
    URA3 (URA3 Budding Yeast)
    yEVenus
    Nat1
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    TEF, TEF2, URA3, bla (E. coli), and pTpA+MCS
    Available Since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127546-plasmid-map-sequence-id-251803

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  37. pINTO_7/8

    Plasmid
    #206431
    Purpose
    A vector backbone (pCC1BAC-based) containing a single SapI recognition site. When digested, it exposes overhangs that allow integration of the vector into the P. tricornutum chloroplast genome.
    Depositor
    Bogumil Karas
    Article
    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:
    Type
    Empty backbone
    Expression
    Bacterial and Yeast
    Available Since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    206431-plasmid-map-sequence-id-419745

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Showing: 521 - 560 of 8173 results