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Showing: 521 - 560 of 7933 results
  1. pMVS142_pACT1_mCherry_Zif268_EBD_MCS_KAN

    Plasmid
    #99049
    Purpose
    Transcription Factor chassis for activation domains. mCherry, Zif268 DNA binding domain, estrogen response domain
    Depositor
    Inserts
    ACT1 promoter (ACT1 Budding Yeast)
    mCherry
    Zif268 DNA binding Domain
    Estrogen response domain (ESR1 Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pGAL-PDE1

    Plasmid
    #160388
    Purpose
    Overxpression of PDE1 in yeast
    Depositor
    Insert
    PDE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pV1090

    Plasmid
    #111427
    Purpose
    gRNA entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at RPS1 (Part of Duet system)
    Depositor
    Insert
    sgRNA-BsmBI stuffer
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. Epac-SH225

    Plasmid
    #191449
    Purpose
    expression of cAMP sensor in yeast
    Depositor
    Insert
    mT2Del-EPACdDEPCD-tdTomato
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. ZJOM82

    Plasmid
    #133648
    Purpose
    Integration of NAB2-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast nucleus.
    Depositor
    Insert
    p1k-NAB2-mCherry (NAB2 Budding Yeast)
    Use
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. p405ADH1

    Plasmid
    #15966
    Insert
    p405ADH1 (ADH1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    ADH1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
  7. pFA6a-PAmCherry-KanMX6

    Plasmid
    #87026
    Purpose
    Tag S. pombe genes in C terminal with PAmCherry
    Depositor
    Insert
    PAmCherry
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Feb. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. pBHM2329

    Plasmid
    #171686
    Purpose
    pRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCR
    Depositor
    Insert
    PCnU6-sgRNA_scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    C. neoformans U6
    Available since
    May 4, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. pAV125

    Plasmid
    #63191
    Purpose
    pRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Oct. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
  10. pCas3

    Plasmid
    #229538
    Purpose
    pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.
    Depositor
    Insert
    Cas3
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae
    Promoter
    Available since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. ZJOM64

    Plasmid
    #133655
    Purpose
    Integration of COX4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.
    Depositor
    Insert
    p1k-COX4-DuDre (COX4 Budding Yeast)
    Use
    Tags
    DuDre
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 23, 2020
    Availability
    Academic Institutions and Nonprofits only
  12. pBHM2641

    Plasmid
    #229525
    Purpose
    Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Insert
    OsTIR1
    Use
    CRISPR
    Tags
    t2a-mNeonGreen-AID*
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    TEF1
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
  13. pBHM2642

    Plasmid
    #229526
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7_opt, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Insert
    OsTIR1
    Use
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
  14. pBHM2644

    Plasmid
    #229528
    Purpose
    Carries AID* tag, empty sgRNA, and NATR marker
    Depositor
    Insert
    AID* - empty sgRNA - NATR
    Use
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
  15. pBHM2645

    Plasmid
    #229529
    Purpose
    Carries mIAA7_opt tag, empty sgRNA, and NATR marker
    Depositor
    Insert
    mIAA7_opt - empty sgRNA - NATR
    Use
    CRISPR
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
  16. pBHM2646

    Plasmid
    #229530
    Purpose
    Carries mAID_opt tag, empty sgRNA, and NATR marker
    Depositor
    Insert
    mAID_opt - empty sgRNA - NATR
    Use
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 521 - 560 of 7933 results