7,942 results

1. pMK425 (GAL-OsTIR1(F74A))

   Plasmid

   #140657

   Purpose

   GAL-OsTIR1(F74A)

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   GAL-OsTIR1(F74A)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDR FLIP43 GPS1

   Plasmid

   #101084

   Purpose

   GA biosensor expression plasmid

   Depositor

   Alexander Jones, Wolf Frommer

   Article

   Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

   Insert

   GPS1
   

   Use

   Tags

   6HIS and cMyc

   Expression

   Yeast

   Mutation

   Promoter

   pPMA1

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pG2J

   Plasmid

   #102882

   Purpose

   Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Inserts

   OsTIR1
   mGFP-AID
   

   Use

   Tags

   AID(71-114)

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 and TEF1

   Available since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PADH1-TIR1-9myc-URA3

   Plasmid

   #207016

   Purpose

   Expresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   TIR1
   

   Use

   Tags

   9xMyc

   Expression

   Yeast

   Mutation

   Promoter

   pADH1

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGR595

   Plasmid

   #213785

   Purpose

   Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.

   Depositor

   Chang Liu

   Insert

   BadBoy2 TP-DNAP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNTI647 dCas9-Mxi1 TetR KanMX

   Plasmid

   #139474

   Purpose

   Integrates CRISPRi effector and tet-responsive regulator

   Depositor

   Nicholas Ingolia

   Article

   McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

   Inserts

   dCas9-Mxi1
   Tet Repressor
   

   Use

   CRISPR

   Tags

   Mxi1 and NLS

   Expression

   Yeast

   Mutation

   D10A, H840A

   Promoter

   pGPM1 and pTEF1

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA6a-link-ymNeongreen-URA3

   Plasmid

   #168059

   Purpose

   Replaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymNeonGreen
   

   Use

   Tags

   ymNeonGreen

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCASB

   Plasmid

   #190175

   Purpose

   Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence

   Depositor

   Jessica Tyler

   Article

   Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:

   Insert

   sgRNA cloning site
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Added 5'-agagacc-3' upstream and 5'…

   Promoter

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. p426MET25_sfpHluorin (MRV55)

   Plasmid

   #115697

   Purpose

   superfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)

   Depositor

   Eckhard Boles

   Article

   Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10

   Insert

   sfpHluorin
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   for introduced mutations see publication

   Promoter

   MET25

   Available since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-link-ymNeongreen-SpHis5

    Plasmid

    #125704

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with SpHIS5 selection

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymNeonGreen
    

    Use

    Tags

    ymNeonGreen

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6a-link-ymScarletI-URA3

    Plasmid

    #168055

    Purpose

    Replaces Addgene plasmid 118457. Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymScarletI
    

    Use

    Tags

    ymScarletI

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-link-yomKate2-SpHis5

    Plasmid

    #44852

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mKate2 with SpHis5 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomKate2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cas9-NAT

    Plasmid

    #64329

    Purpose

    Cas9 expression cassette carried by a yeast single-copy episome plasmid

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA6a-link-ymScarletI-SpHis5

    Plasmid

    #219844

    Purpose

    tagging vector to tag genes with ymScarletI and a HIS5 gene

    Depositor

    Bas Teusink

    Insert

    ymScarletI-SpHis5
    

    Use

    Tags

    ymScarletI

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pDIV270

    Plasmid

    #226181

    Purpose

    Expresses Cas9 and gRNA targeting KU70 gene in K. phaffii.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

    Insert

    tRNA-sgRNA-tRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TEF1p (Komagataella phaffii)

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDIV151

    Plasmid

    #226180

    Purpose

    Expresses Cas9 in a backbone compatible with K. phaffii.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    GAP1

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDRF1 Cyterm-ymStayGold

    Plasmid

    #219855

    Purpose

    expression of Cyterm-ymStayGoldd in budding yeast

    Depositor

    Bas Teusink

    Insert

    Cyterm-ymStayGold
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pETCON-HtsptLB12v.3

    Plasmid

    #45121

    Depositor

    David Baker

    Article

    Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.

    Insert

    HtsptLB12v.3
    

    Use

    Tags

    Aga2p for yeast surface display and c-myc epitope…

    Expression

    Yeast

    Mutation

    Promoter

    GAL

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMitoLOC

    Plasmid

    #58980

    Purpose

    Expression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrila

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Mitochondrion. 2015 Sep;24:77-86. doi: 1

    Inserts

    mCherry
    GFP
    

    Use

    Tags

    preCOX4 and preSU9

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 and MET17

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGI3EM17

    Plasmid

    #210569

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    SpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter
    

    Use

    Luciferase

    Tags

    NanoLuc luciferase

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpH2Bpro

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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