8,350 results
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Plasmid#139426PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.DepositorInsertmNeonGreen
ExpressionYeastPromoterTEF1Available SinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE46
Plasmid#139425PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.DepositorInsertmRuby3
ExpressionYeastPromoterTEF1Available SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKmK.C3
Plasmid#125061PurposeStorage plasmid for K.marxianus centromereDepositorInsertKmARS/CEN6
UseSynthetic BiologyExpressionYeastAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
TDH3-STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)LexAVP16-tCYC
Plasmid#135465PurposeMembrane-anchored TF used for yeast directed evolution of TEV proteaseDepositorInsertSTE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)-LexAVP16
TagsBFP, flagExpressionYeastPromoterTDH3Available SinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I3R
Plasmid#125065PurposeStorage plasmid for K.marxianus right homology armDepositorInsertRight homology arm for targeting integration to SWF1/ARO1
UseSynthetic BiologyExpressionYeastAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I1R
Plasmid#125063PurposeStorage plasmid for K.marxianus right homology armDepositorInsertRight homology arm for targeting integration to LAC4
UseSynthetic BiologyExpressionYeastAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV126
Plasmid#63192PurposepRS426 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I1L
Plasmid#125030PurposeStorage plasmid for K.marxianus left homology arm; type 1 partDepositorInsertLeft homology arm for targeting integration to LAC4
UseSynthetic BiologyExpressionYeastAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I3L
Plasmid#125032PurposeStorage plasmid for K.marxianus left homology arm; type 1 partDepositorInsertLeft homology arm for targeting integration to SWF1/ARO1
UseSynthetic BiologyExpressionYeastAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
EMC1-2EGFP-ter-NAT
Plasmid#184763PurposeMarker for yeast endoplasmic reticulum (ER). Integration of 2xGFP tag at EMC1 C terminus. Uses antibiotic resistance marker natMX6.DepositorAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
p426*-SaSgH
Plasmid#136994PurposeHelper plasmid for SaCas9 gRNA cloning and expressionDepositorTypeEmpty backboneExpressionYeastPromoterSNR52Available SinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWKS11
Plasmid#139418PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mNeonGreen marker.DepositorInsertmNeonGreen
ExpressionYeastPromoterTEF1Available SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM27
Plasmid#133641PurposeIntegration of GFP-PHO8 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast vacuole.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS315e_pGal-nCas9(D10A)-PmCDA1
Plasmid#79617PurposeExpresses nCas9(D10A)-PmCDA1 in yeast cellsDepositorInsertSpCas9
TagsPmCDA1ExpressionYeastMutationD10A for nickase Cas9PromoterpGal1Available SinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-FLAG-HIS3MX6
Plasmid#20750DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable SinceMarch 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fSNAP-NAT
Plasmid#188916PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fSNAP-NAT
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fCLIP-NAT
Plasmid#188921PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fCLIP-NAT
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pE2c
Plasmid#17462Purposemodified pENTR vector for C-terminal 3x HA tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTags3xHAExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
Gal4BD-CRY2(535)
Plasmid#75369PurposeYeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)DepositorAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB6633
Plasmid#106162PurposeEasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6677 into Yarrowia lipolytica chromosomal location IntE_1, amp resistanceDepositorInsertunknown
ExpressionYeastAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-ccdB-EYFP
Plasmid#14219DepositorTypeEmpty backboneUseGateway CloningTagsEYFPExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMTU-DO-LEU-C4
Plasmid#160341PurposeHigh-copy expression vector for K. marxianus, leucine selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFTK048
Plasmid#171320PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertDsRed.T1-SKL fluorescent reporter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherry
Plasmid#68280PurposeSKARS: Mpk1 MAPK activity sensor: Mkk2DS (1-100) 2xNLS mCherryDepositorAvailable SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHUK
Plasmid#64183Purposeharbours marker genes HIS3, URA3 and LYS2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
YCPlac33-sGFP-VRG4
Plasmid#25447DepositorInsertSynthetic Green Fluorescent Protein - VRG4
TagsGFPExpressionYeastAvailable SinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pV1025
Plasmid#111424PurposeExpression of Candida albicans codon-optimized Cas9 (CaCas9) - integrates at ENO1 (Part of Duet system)DepositorInsertCaCas9
UseCRISPRExpressionYeastAvailable SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
N-GFP-Dcp2
Plasmid#163585Purposeexpress N-terminal GFP tagged Dcp2 FL at endogenous level in S.cerevisiaeDepositorAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
ZJOM135
Plasmid#133659PurposeIntegration of SEC63-2mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).DepositorAvailable SinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G3-Km
Plasmid#46817PurposeTEF1 and GAL10 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneTagsFLAG and c-mycExpressionYeastPromoterTEF1 and GAL10Available SinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFS555
Plasmid#242855PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetSW4 promoter at its endogenous locusDepositorInsertsTetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW4 promoterAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
NM R2E2 GFP
Plasmid#1208DepositorInsertsup35 NM domain (SUP35 Budding Yeast)
TagsGFPExpressionYeastMutation2 additional copies of second oligopeptide repeat…Available SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-tdTomato-hphMX6
Plasmid#105157PurposeYeast gene targetingDepositorInserttdTomato-hphMX6
TagstdTomatoExpressionBacterial and YeastAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
BS-His3
Plasmid#69197Purposecomplementation of yeast auxotrophic marker HIS3DepositorInsertHIS3
ExpressionYeastAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUG34-NES-EGFP-cPHx3
Plasmid#183669PurposePtdIns(3,4)P2 biosensor for expression in yeastDepositorInsertPLEKHA1 (PLEKHA1 Frog, Human)
TagsX. laevis map2k1.L(32-44)-EGFPExpressionYeastMutationAmino acids 169-329 (tandem trimer)PromoterMET25Available SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL-RPB1
Plasmid#10981DepositorAvailable SinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJKW1148
Plasmid#119029PurposeYeast expression plasmid for PmCYP719A26DepositorInsertPmCYP719A26
ExpressionYeastPromoterGAL10Available SinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS416-HIS3p-TID-FKBP-HA3
Plasmid#228006PurposeExpresses TurboID-FKBP fusion from HIS3 promoter; yeast BioIDDepositorInsertTurboID-FKBP
TagsHA tagExpressionYeastPromoterHIS3Available SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FKBP-3HA-KanMX6
Plasmid#228024PurposeTo integrate TID-FKBP fusion at yeast promoter; yeast BioID with KanMX6 markerDepositorInsertTurboID-FKBP
TagsHA tagExpressionYeastAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pREP3-TEV-NLS
Plasmid#21146DepositorInsertTEV protease (TEVgp1 tobacco etch virus)
UseS. pombeTags2xNLS, NLS, and mycExpressionYeastAvailable SinceJuly 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLH_Scr15
Plasmid#100537PurposeCEN/ARS plasmid with expression cassettes for PhyBNT-CreN and PIF3-NLS-CreC fusion proteins for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.DepositorUseCre/Lox and Synthetic BiologyTagsCreC, CreN, and SV40 NLSExpressionYeastMutationN-terminal version of PhyB (AA 1-621)/ Mutation o…PromoterFBA1 and TDH3Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
p416 72Q GPD
Plasmid#1179DepositorAvailable SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-MFA-NFAPoptim
Plasmid#221090PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ERDepositorTypeEmpty backboneExpressionYeastAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGal4AD-CIBN
Plasmid#28246PurposeExpresses Gal4 Activation Domain fused to CIBN (aa1-170) for blue-light-induced transcription activationDepositorInsertCIB1 (1-171aa)
TagsGal4ADExpressionYeastAvailable SinceMarch 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAV0328
Plasmid#133489PurposeExpression of nucleo-cytoplasmic mCherryDepositorInsertpUra4(AfeI)-p(act1)-mCherry-terminator(tdh1)
ExpressionYeastAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACT-Lex
Plasmid#183149PurposeYeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)DepositorInsertGAL4 AD
UseYeat two-hybridTagsHA tagExpressionYeastAvailable SinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pV1393:SpRY
Plasmid#172189PurposeGenome editing of C. albicansDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
426Gal-FUS
Plasmid#29591DepositorInsertFUS (FUS Human)
ExpressionYeastAvailable SinceJuly 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJT1
Plasmid#98299PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoterDepositorInsertPTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
ExpressionYeastAvailable SinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRG713_lexO-HO_LexA-TF_URA3MX
Plasmid#154821PurposeInducible expression of HO endonuclease in S. cerevisiaeDepositorInsertsTagsFLAGExpressionYeastPromoterACT1 and lexO_4-CYC1_coreAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only