8,265 results
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Plasmid#105157PurposeYeast gene targetingDepositorInserttdTomato-hphMX6
TagstdTomatoExpressionBacterial and YeastAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS428
Plasmid#11257DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDS275
Plasmid#34972PurposeExpresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A mutations).DepositorInsertMid2(SS/TM)-GFP-LOVpep
ExpressionYeastMutationT406A, T407A, I532AAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
linker-mCitrine-PIF6-NAT
Plasmid#51576PurposeTag protein of interest with PIF6 in Saccharomyces cerevisiaeDepositorInsertPIF6 (PIL2 Mustard Weed)
Tagslinker and mCitrineExpressionYeastMutation1-100aa of PIF6PromoternoneAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
MS2-Snapf
Plasmid#65860PurposeExpresses RNA-binding protein MS2 with fast SNAP fusion tagDepositorInsertsMS2
Fast SNAP
TagsHA and NLSExpressionBacterial and YeastAvailable SinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pE2c
Plasmid#17462Purposemodified pENTR vector for C-terminal 3x HA tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTags3xHAExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
JME4472
Plasmid#129658PurposeURA3ex_CrisprCas9-yl_RFP: CAS9 vector with URA3ex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYZ158
Plasmid#98414PurposePombe Expression Vector - nmt1 promoter with Sp.leu1 markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-sfGFP (pJJF001)
Plasmid#173457PurposeC-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timerDepositorInsertmCherry-sfGFP
ExpressionYeastPromoterno promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
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ZJOM43
Plasmid#133652PurposeIntegration of VPS4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.DepositorAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
p426*-SpSgH
Plasmid#136993PurposeHelper plasmid for SpCas9 gRNA cloning and expressionDepositorTypeEmpty backboneExpressionYeastPromoterSNR52Available SinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGR618
Plasmid#213786PurposeIntegrates at the CAN1 locus the BB-Tv TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassetteDepositorInsertBB-Tv TP-DNAP1
UseSynthetic BiologyExpressionBacterial and YeastAvailable SinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pRS315e_pGal-nCas9(D10A)-PmCDA1
Plasmid#79617PurposeExpresses nCas9(D10A)-PmCDA1 in yeast cellsDepositorInsertSpCas9
TagsPmCDA1ExpressionYeastMutationD10A for nickase Cas9PromoterpGal1Available SinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
GAL FLAG103QDProGFPp303
Plasmid#15587DepositorInserthtt 103Q Delta Pro (HTT Human)
TagsFLAG and GFPExpressionYeastMutationProline-rich region removedAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pM-TagRFP657
Plasmid#176563PurposeExpression of TagRFP657 for auxotrophic selection in the absence of methionineDepositorInsertTagRFP657
ExpressionYeastPromoterTDH1(GAP)Available SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK-DN6
Plasmid#180287PurposeHygR multi-gene yeast expression vector (GFP-dropout)DepositorInsertConLS' - GFP - ConRE' - HygR - CEN6/ARS4
ExpressionBacterial and YeastAvailable SinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGal4AD-CIBN
Plasmid#28246PurposeExpresses Gal4 Activation Domain fused to CIBN (aa1-170) for blue-light-induced transcription activationDepositorInsertCIB1 (1-171aa)
TagsGal4ADExpressionYeastAvailable SinceMarch 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-SEC7-mCherry2Bx6
Plasmid#105267PurposePlasmid for pop-in/pop-out replacement of Sec7 with Sec7-mCherry2Bx6.DepositorAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYES260-sc.eEF2 K509A
Plasmid#85120Purposeyeast expression of mutant EEF2DepositorTags6xHisExpressionYeastMutationK509APromoterGAL1Available SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
3396 pBM272 Bax
Plasmid#8770DepositorInsertBax (Bax Mouse)
ExpressionYeastAvailable SinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGWKS9
Plasmid#139416PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mTurquoise2 marker.DepositorInsertmTurquoise2
ExpressionYeastPromoterTEF1Available SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM17
Plasmid#210569PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastPromoterSpH2BproAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJW1661
Plasmid#112035PurposeOsTIR1 expression cassette marked with URADepositorInsertTIR1
ExpressionYeastAvailable SinceSept. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMTKM07
Plasmid#233479PurposeSpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and URA selection.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDEST-DHFR F[3]-C (LEU2)
Plasmid#177796PurposeGateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.DepositorTypeEmpty backboneTagsDHFR F[3]ExpressionYeastPromoterADH1Available SinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-DHFR F[1,2]-C (TRP1)
Plasmid#177795PurposeGateway destination vector to insert genes of interest having a C-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.DepositorTypeEmpty backboneTagsDHFR F[1,2]ExpressionYeastPromoterADH1Available SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSP568
Plasmid#139493PurposeInsertion of the pSTL1 promoter and 24 MS2 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpSTL1 24xMS2sl
ExpressionYeastMutationWTPromoterpSTL1Available SinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-VPH1-mEGFP
Plasmid#105270PurposePlasmid for pop-in/pop-out replacement of Vph1 with Vph1-mEGFP.DepositorAvailable SinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRG705_lexO-AsiSI_LexA-TF_LEU2MX
Plasmid#154817PurposeInducible expression of AsiSI endonuclease in S. cerevisiaeDepositorInsertsAsiSI endonuclease
LexA-ER-B112
TagsFLAG and NLSExpressionYeastPromoterACT1 and lexO_4-CYC1_coreAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
p416 72Q GPD
Plasmid#1179DepositorAvailable SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pFLAG UBR1 SBX
Plasmid#24506DepositorAvailable SinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPICZA_CeTRIC-B1_Del
Plasmid#87344PurposeInducible expression of CeTRIC-B1 in P.Pichia strainsDepositorInsertTRIC-B1
TagspolyhistidineExpressionYeastMutationΔ248-297PromoterAOX1Available SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS423-CUP1-His-Ubi
Plasmid#99541PurposeYeast episomal vector for expression of His-tagged ubiquitin; HIS3 markerDepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-EGFP
Plasmid#166140PurposeEGFP expression under the control of the Galactose-inducible promoter yeast. Contains CEN/ARS element for low copy within yeast.DepositorInsertEGFP
ExpressionYeastPromoterGAL1Available SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp LYS2-LEU2
Plasmid#122909PurposeLYS2-LEU2 integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable SinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-FKBP(M)x4
Plasmid#85003PurposeExpresses EGFP-FKBP(M)x4 in yeastDepositorInsertEGFP-FKBP(M)x4
ExpressionYeastAvailable SinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPL5606_TEF1*-Cre_ADE2
Plasmid#60932PurposeConstitutive expression vector for Cre-recombinase containing ADE2 selection marker.DepositorInsertCre
TagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastPromotermutant TEF1Available SinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFS561
Plasmid#242861PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetS promoter at its endogenous locusDepositorInsertsTetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetS promoterAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFS562
Plasmid#242862PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetSW1 promoter at its endogenous locusDepositorInsertsTetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW1 promoterAvailable SinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFS564
Plasmid#242864PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetSW3 promoter at its endogenous locusDepositorInsertsTetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW3 promoterAvailable SinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFS565
Plasmid#242865PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetSW4 promoter at its endogenous locusDepositorInsertsTetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW4 promoterAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-MFA-NFAPoptim
Plasmid#221095PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ERDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT899
Plasmid#228288PurposeConstitutive mutant rPhlTA expression with strong promoterDepositorInsertrphlTA mutant
UseSynthetic BiologyTagsThree copies of VP16 (VP48)-Nuclear localization …ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143KPromoterKpGAPDH promoterAvailable SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal TDP43 WT
Plasmid#27458DepositorInsertTDP-43 (TARDBP Human)
ExpressionYeastAvailable SinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
p404-MTS-mCherry-GFP1-10
Plasmid#91958PurposeExpresses mCherry-GFP1-10 in yeast cell mitochondriaDepositorInsertMTS-mCherry-GFP1-10
ExpressionYeastPromoterGAPAvailable SinceJune 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAV106-pTDH3-mCherry-10xSumo-6xSIM
Plasmid#116928PurposeYeast integrative vector for expression of the SUMO-SIM complexDepositorInsert10xSUMO-6xSIM
TagsmCherryExpressionYeastPromoterTDH3Available SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GBP-kanMX6
Plasmid#89067Purposegenomic GBP epitope C-terminal tagging in S. pombeDepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsGBPExpressionYeastAvailable SinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G1-Ph
Plasmid#46814PurposeTEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformationDepositorTypeEmpty backboneTagsFLAG and c-mycExpressionYeastPromoterTEF1 and PGK1Available SinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only