7,933 results
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Plasmid#217315PurposeGAL7(p)-mRuby3:amdS fusion reporterDepositorInsertAcetamidase
UseTagsmRuby3ExpressionYeastMutationPromoterGAL7Available sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSPR31
Plasmid#217316PurposeTEF1(p)-mRuby3:amdS fusion reporterDepositorInsertAcetamidase
UseTagsmRuby3ExpressionYeastMutationPromoterTEF1Available sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSPR32
Plasmid#217317PurposeGAL7(p)-mNeonGreen:amdS fusion reporterDepositorInsertAcetamidase
UseTagsmNeonGreenExpressionYeastMutationPromoterGAL7Available sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSPR33
Plasmid#217318PurposeTEF1(p)-mNeonGreen:amdS fusion reporterDepositorInsertAcetamidase
UseTagsmNeonGreenExpressionYeastMutationPromoterTEF1Available sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-Ura3Kl
Plasmid#69195Purposecomplementation of yeast auxotrophic marker URA3(Kluyveromyces lactis)DepositorInsertKluyveromyces lactis URA3
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
RIB VP64 R1
Plasmid#161493PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R1_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting R1 in the RIB1 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJZC588
Plasmid#62315PurposesgRNA with 2x MS2 (wt+f6) for yeast cellsDepositorInsertsgRNA + 2x MS2 (wt+f6) binding module
UseTagsExpressionYeastMutationPromoterSNR52Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSP-G1
Plasmid#64736PurposeYeast expression plasmidDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
bRA66
Plasmid#100952PurposeGAL1 driven Cas9. gRNA can be cloned into BplI sites.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-ADH700-yeCherry-pTEF1 (S.C.)-yeGFP-DHFR
Plasmid#24584DepositorInsertpADH1 yeCherry::pTEF1 (S.C.)yeGFP-DHFR
UseTagsExpressionYeastMutation1. Truncated version of ADH700 promoter (SbfI/Spe…PromoterAvailable sinceMay 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFM689
Plasmid#74635Purposealias pFA6a-mCherry-hphNT1 - vector for C-terminal tagging of yeast genes with mCherry and hygromycin selection markerDepositorInsertsUseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDN0604 (pDEST, HygR)
Plasmid#210483PurposepDEST vector with HygR marker for gene expression in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN0603 (pDEST, NatR)
Plasmid#210482PurposepDEST vector with NatR marker for gene expression in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCu-MCP-2yeGFP(405)
Plasmid#121419PurposeExpressing MCP-2yeGFPDepositorInsertCUP1p-MCP-2yeGFP
UseTagsExpressionYeastMutationPromoterCUP1 promoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM20C
Plasmid#135487PurposeAgrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hphDepositorInserthph SpunH2A/Bpr H2B-tdTomato
UseTagstdTomatoExpressionBacterial and YeastMutationPromoterSpunH2A/Bpr (divergent)Available sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
YEp181-CUP1-His-Smt3
Plasmid#99536PurposeYeast episomal vector for expression of His-tagged Smt3 (SUMO); LEU2 markerDepositorInsertSMT3 (SMT3 Budding Yeast)
UseTags7xHisExpressionYeastMutationPromoterCUP1Available sinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAV0620
Plasmid#133506PurposeExpression of eGFP tagged PCNA to monitor DNA replicationDepositorInsertpUra4(AfeI)-p(pcn1)-eGFP-pcn1-terminator(nmt1)-natMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDS220
Plasmid#34980PurposeExpresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.DepositorInsertePDZb–mCherry
UseTagsExpressionYeastMutationPromoterTEFAvailable sinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pE2n
Plasmid#17456Purposemodified pENTR vector for N-terminal 3x HA tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTags3xHAExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRSCupSup35GFP
Plasmid#1087DepositorInsertSUP35 (SUP35 Budding Yeast)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceAug. 9, 2005AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-His-tag-kanMX6
Plasmid#20763DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionYeastMutationPromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ura4MX6
Plasmid#49184Purposegene deletion in S. pombeDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-His-tag-hphMX4
Plasmid#20764DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionYeastMutationPromoterAvailable sinceMarch 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCfB2794
Plasmid#63651PurposepCfB2794 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pI1-MTU-DO-G418
Plasmid#160012PurposeIntegrative vector for K. marxianus, targeting the LAC4 locus. G418 selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
p404GAL1
Plasmid#17417DepositorInsertGAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGAL1 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
ZJOM5
Plasmid#133635PurposeIntegration of CHS5-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.DepositorInsertp1k-CHS5-GFP (CHS5 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mTID-HA3-KanMX6
Plasmid#227982PurposeTo insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID, KanMX6 markerDepositorInsertminiTurboID
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
p416 25Q GAL
Plasmid#1185DepositorInserthtt 25Q (HTT Human)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMTS-hFluc
Plasmid#177716PurposeFirefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targetingDepositorInserthFluc
UseTagsMitochondrial Targeting Sequence MTS SSExpressionBacterial, Mammalian, and Y…MutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pJW1664
Plasmid#112038PurposeCEN/ARS plasmid expression of mitochondrial Su9-TagBFP marked with HISDepositorInsertSu9
UseTagsTagBFPExpressionYeastMutationamino acids 1-76PromoterAvailable sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCUPI NPD-GFP
Plasmid#1191DepositorInsertN-terminus of Sup35 (SUP35 Budding Yeast)
UseCup1 promoterTagsGFPExpressionYeastMutationfused N-terminal Sup35 (~1-253aa) to GFPPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMZ379
Plasmid#74215PurposeCombined sgRNA/Cas9 vector with NatMX markerDepositorInsertsCas9
rrk1:sgRNA
UseCRISPRTagsExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-Alpha-synuclein-A53T
Plasmid#181722PurposeGalactose inducible expression of Alpha-synuclein-A53TDepositorInsertAlpha-synuclein (SNCA Human)
UseTagsExpressionYeastMutationA53TPromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-fCLIP-3xHA
Plasmid#188936PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertURA-GAL1-fCLIP-3xHA
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fCLIP-NAT
Plasmid#188921PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fCLIP-NAT
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH3FS
Plasmid#85780PurposeN-ICE plasmid pH3FSDepositorInsertloxP-HphMX-loxP-3FLAG
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLexA
Plasmid#11342DepositorTypeEmpty backboneUseTagsLexA DNA Binding DomainExpressionYeastMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PHO5-GFP-Osh1
Plasmid#58835PurposeExpresses GFP-Tagged Osh1 in yeastDepositorUseTagsGFPExpressionYeastMutationPromoterPHO5Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS712
Plasmid#213041PurposeCRISPR activation based overexpression in Nakaseomyces (Candida) glabrataDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWS3867
Plasmid#185744PurposeSub-array backbone 3/4DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT AID*-GFP osTIR1
Plasmid#182514PurposeC-SWAT compatible vector for C-terminal tagging with AID*-GFP for auxin inducible gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration.DepositorInsertAID*
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG303-Gal-GA50
Plasmid#84907Purposeinduce expression of dipeptide repeat GA in yeastDepositorInsertGA50 (C9orf72 Synthetic)
UseTagsFLAG and MycExpressionYeastMutationPromoterGAL1Available sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTCW012
Plasmid#83527PurposeCloning destination vector for phermone mediated expression from the FUS1J2 promoter and CYC1 terminatorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pScDD2_ERdd
Plasmid#109047Purposedestabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).DepositorInsertER50 (ESR1 H. sapiens gene with yeast enhanced codon)
UseTagsyeast enhanced GFP with 8 amino acids TSGRGEGQ li…ExpressionYeastMutation3 mutations : E380G, R434G, N532SPromoterSOD1Available sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMTU-DO-URA-C4
Plasmid#160339PurposeHigh-copy expression vector for K. marxianus, uracil selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pI3-MTU-DO-G418
Plasmid#160023PurposeIntegrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). G418 selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Osa-pCAMBIA-1301-STTM319
Plasmid#84201PurposeDisturb the expression of miR319 in riceDepositorInsertmiR319
UseTagsExpressionBacterial, Plant, and Yeast…MutationPromoter2x35SAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
3413 pBF339 Bcl-XL
Plasmid#8773DepositorInsertBcl-XL (Bcl2l1 Mouse)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only