8,173 results
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Plasmid#118599PurposeExpresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in miceDepositorInsertMus musculus sphingosine kinase 1
TagsFLAGExpressionYeastPromoterGAL1, 10Available SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-Dcr1
Plasmid#22307DepositorInsertDCR1
Use2micronExpressionYeastAvailable SinceOct. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAT222
Plasmid#40942DepositorInsertGFP-LacI**
TagsGFP(S65T, V163A) and SV40 NLSExpressionYeastMutationLacI**/LacI2G(4 mutations) (see comment below); lā¦PromoterHis3 promoter fragmentAvailable SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ube2b
Plasmid#31408DepositorAvailable SinceOct. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pJS646
Plasmid#183175PurposeY2H vector compatible with pJS647 PYR1 insertDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
HsSPHK1
Plasmid#118091PurposeExpresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)DepositorInsertHomo sapiens Sphingosine Kinase 1
TagsFLAGExpressionYeastPromoterGAL1, 10Available SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymScarletI-T2A-mTurquoise2
Plasmid#118441PurposeProduces equimolar expression of yeast codon-optimized mScarletI and mTq2DepositorInsertymScarletI-T2A-mTurquoise2
ExpressionYeastAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-Strep-tagII-HIS3MX6
Plasmid#20756DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable SinceMarch 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
PADH1-TIR1-9myc-URA3
Plasmid#207016PurposeExpresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).DepositorInsertTIR1
Tags9xMycExpressionYeastPromoterpADH1Available SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYX233-b2delta-DHFR
Plasmid#163760PurposeGal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)delta(19)-DHFRDepositorInsertb2delta-DHFR (Dhfr Budding Yeast, Mouse)
ExpressionYeastMutationAmino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fuā¦PromoterGAL1Available SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ube2r1
Plasmid#31426DepositorAvailable SinceOct. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-HALO-URA
Plasmid#188932PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-HALO-URA
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
POM43-mTFP1
Plasmid#69853PurposeN-terminal tagging with mTFP1 using LEU2 as a selection marker in S. cerevisiae.DepositorInsertmTFP1 minus start and stop codon
ExpressionYeastAvailable SinceApril 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ube2s
Plasmid#31417DepositorAvailable SinceOct. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 Cyterm-ymStayGold
Plasmid#219855Purposeexpression of Cyterm-ymStayGoldd in budding yeastDepositorInsertCyterm-ymStayGold
ExpressionYeastAvailable SinceAug. 27, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJB06T
Plasmid#16008DepositorInsertACT1pr-DsRed.T1.N1
ExpressionYeastAvailable SinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pU
Plasmid#64180Purposeharbours marker gene URA3DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEM112B
Plasmid#210602PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Ater-mClover3-SpH2Apro | SpH2Bpro-GP2A-GT2A-Hshph-Synter8
TagsmClover3ExpressionBacterial and YeastPromoterSpH2A/Bpro (divergent)Available SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ube2t
Plasmid#31427DepositorAvailable SinceOct. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymScarletI
Plasmid#118452PurposeOverexpression of yeast codon-optimized mScarletIDepositorInsertymScarletI
ExpressionYeastAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHCT2
Plasmid#130970PurposeHyPr plasmid with natMX markerDepositorInsertempty backbone
ExpressionYeastAvailable SinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHMK34
Plasmid#130971PurposeHyPr plasmid with hphMX markerDepositorInsertempty backbone
ExpressionYeastAvailable SinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFM699
Plasmid#74636Purposealias pFA6a-mCherry-natNT2 - vector for C-terminal tagging of yeast genes with mCherry and nourseothricin selection markerDepositorInsertmCherry
ExpressionYeastPromoternoneAvailable SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUNIV-EGFP
Plasmid#24706DepositorInsertEGFP (eGFP Aequoria victoria)
ExpressionBacterial, Mammalian, and Yā¦Available SinceDec. 6, 2010AvailabilityAcademic Institutions and Nonprofits only -
p414GPD_Hsp82viiB
Plasmid#61709PurposeYeast plasmid with Hsp82 heterodimer construct.DepositorInsertHsp82viiB (HSP82 Budding Yeast)
Tags6xHisExpressionYeastMutationHeteromeric coiled coil inserted after amino acidā¦PromoterGPD1Available SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHOHO2_Hsp82viiA
Plasmid#61708PurposeYeast integration plasmid targeted to the HO loci with heterodimeric Hsp82. Digest with NotI prior to addition to yeast.DepositorInsertHsp82viiA (HSP82 Budding Yeast)
UseYeast integration vector targeting inserts to theā¦Tags6xHisExpressionYeastMutationHeteromeric coiled coil inserted after amino acidā¦PromoterTEF1Available SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX-(3ĆFLAG)-TEV-ProtA
Plasmid#52692PurposeFor C-terminal tagging of ORFs with a (3ĆFLAG)-TEV-ProtADepositorInsert(3ĆFLAG)-TEV-ProtA
ExpressionYeastPromotern/aAvailable SinceApril 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG
Plasmid#158127PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertmTagBFP
ExpressionYeastPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBBK94 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (KanR)
Plasmid#179078PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
416Gal-FUS-YFP
Plasmid#29593DepositorAvailable SinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS305-mAID-Nb(VHHGFP4)
Plasmid#198412PurposeExpress mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeastDepositorInsertmAID-Nb (VHHGFP4)
ExpressionYeastPromoterADH1 promoterAvailable SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBKWH-p53
Plasmid#133900PurposePositive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWHDepositorAvailable SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG305GPD-ccdB
Plasmid#14138DepositorTypeEmpty backboneUseGateway destinationExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
Linked Free ClLIS1 (M)
Plasmid#182487PurposeYeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-ClLIS1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertFPPS(M)-LS
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP38-AtLEA4-5
Plasmid#178189PurposeSED1 biosensor for expression in Saccharomyces cerevisiaeDepositorInsertSED1 biosensor
ExpressionYeastPromoterpPMA1Available SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCYC2
Plasmid#43970DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastPromoterCYC1Available SinceApril 9, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-link-yoTagRFP-T-CaURA3
Plasmid#44877PurposeYeast tagging vector to create gene fusion with yeast optimized TagRFP-T with CaUra3 selectionDepositorInsertyoTagRFP-T
ExpressionYeastAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFTK055
Plasmid#171327PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertrTA2S-M2 (TetR-3xVP16) transcriptional activator
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yEmCFP-Hygro
Plasmid#44644DepositorInsertyEmCFP
ExpressionYeastMutationA206RAvailable SinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKT0128
Plasmid#8729DepositorInsertyEGFP
TagsCodon optimized linkerExpressionYeastAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRG207
Plasmid#64530PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pY2-MET-IV
Plasmid#218117PurposeMET-HYGRO plasmid (218133) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY2-LYS-IV
Plasmid#218118PurposeLYS-Leu2 plasmid (218114) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG305GAL-ccdB
Plasmid#14137DepositorTypeEmpty backboneUseGateway destinationExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGS_129
Plasmid#160652PurposeExpresses Human APOE4 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoterDepositorInsertApolipoprotein E e4 (APOE Human)
TagsKar2 signal sequenceExpressionYeastMutatione4 allelePromoterpZ estradiol inducible promoterAvailable SinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVTU-Dest
Plasmid#105088PurposeYeast expression vector for Gateway cloning and SUS bridge assaysDepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZJOM57
Plasmid#133656PurposeIntegration of PEX3-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast peroxisome.DepositorAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-ADH700-yeCherry-p150-yeGFP-DHFR
Plasmid#24378DepositorInsertpADH1 yeCherry::p150yeGFP-DHFR
ExpressionYeastMutation1. Truncated version of ADH700 promoter (SbfI/Speā¦Available SinceMay 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGAN230
Plasmid#131122Purposereverse yTRAP, RNQ1-aTF reporting on tetR-mKate2DepositorTypeEmpty backboneExpressionYeastPromoterSUP35Available SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only