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Showing: 1 - 40 of 11635 results

  3. pCRISPRyl

    Plasmid
    #70007
    Purpose
    CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al ACS Synth Biol. 2015 Dec 29.
    Inserts
    Codon optimized Cas9 from S. pyogenes
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    70007-plasmid-map-sequence-id-137578

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  5. p5-71

    Plasmid
    #118085
    Purpose
    N-29-1 split N-terminal T7 RNAP variant-linker-ZA expression plasmid
    Depositor
    Bryan Dickinson
    Article
    Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d
    Insert
    N-29-1 split N-terminal T7 RNAP variant-linker-ZA expression plasmid
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    118085-plasmid-map-sequence-id-227374

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  10. pCK302

    Plasmid
    #87768
    Purpose
    As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Inserts
    PrhaBAD-sfGFP
    rhaS from E. coli with its native RBS from E. coli
    Use
    Synthetic Biology
    Tags
    6xHis Tag
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaBAD rhamnose-inducible promoter from E. coli …
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87768-plasmid-map-sequence-id-171131

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  19. pJBL703

    Plasmid
    #140373
    Purpose
    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 2BP dowsntream from the promoter
    Depositor
    Julius Lucks
    Article
    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038
    Insert
    T7-2BP-tetO-3WJdB-T
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140373-plasmid-map-sequence-id-297839

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  23. pHK352.3

    Plasmid
    #235473
    Purpose
    Message phagemid carrying sgRNA3 (prom. J23110, backbone RSF1030)
    Depositor
    Yolanda Schaerli
    Article
    et al Nat Commun 16, 3569 (2025)
    Insert
    sgRNA3
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    235473-plasmid-map-sequence-id-469104

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  24. pCAROTENE5X

    Plasmid
    #208816
    Purpose
    Encodes bacterial crtEBIY operon under the control of synthetic promoter responsive to JUB1-derived artificial transcription factor in bacterial cell
    Depositor
    Marc Erhardt
    Article
    Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:
    Insert
    Five copies of JUB1 binding site within the synthetic promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    JUB1 TF-responsive promoter
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    208816-plasmid-map-sequence-id-420948

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  26. pKB256

    Plasmid
    #170039
    Purpose
    Encodes LacI-controlled expression of surface-displayed Bac2a and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    Bac2a
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170039-plasmid-map-sequence-id-336759

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  30. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

    Plasmid
    #73440
    Purpose
    Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    PG6-vioABE-P4A6-vioC-P3A2-vioD
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PG6, P4A6, and P3A2 (orthogonal T7-lac variants)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73440-plasmid-map-sequence-id-135339

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  31. AAVS1-iSAM

    Plasmid
    #211495
    Purpose
    AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa
    Depositor
    Antonella Fidanza, Lesley Forrester
    Article
    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7
    Inserts
    UniSAM insert
    TET-ON
    Neo Resistance
    Insulator
    Use
    CRISPR and Synthetic Biology
    Tags
    T2A-mCherry Tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a, NA, Splicing acceptor, and TREGV promoter
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    211495-plasmid-map-sequence-id-454077

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  34. pOpen-TEV protease

    Plasmid
    #165509
    Purpose
    Used for site-specific cleavage of proteins. TEV cleaves the protein consensus sequence EXXYXQ^S/G
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    TEV protease
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165509-plasmid-map-sequence-id-338590

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  39. pCDNA3.2/V5-DEST-Noa1

    Plasmid
    #191969
    Purpose
    Overexpression of Nitric Oxide Associated 1 protein (NOA1), tagged with V5.
    Depositor
    Luca Scorrano
    Insert
    Noa1
    Use
    Synthetic Biology
    Tags
    V5
    Expression
    Mutation
    Promoter
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    191969-plasmid-map-sequence-id-379647

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Showing: 1 - 40 of 11635 results