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169,885 results

Showing: 181 - 200 of 169885 results

  1. pDest-Sumo

    Plasmid
    #106980
    Purpose
    LR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tag
    Depositor
    Han Choe
    Article
    Nguyen et al Sci Rep. 2017 Nov 23;7(1):16139. doi: 10
    Type
    Empty backbone
    Tags
    His8-Sumo
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    106980-plasmid-map-sequence-id-204719

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  3. lenti-sgRNA puro

    Plasmid
    #104990
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Article
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104990-plasmid-map-sequence-id-443800

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  4. TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone

    Plasmid
    #216154
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic exon and C-terminal FLAG
    Expression
    Mammalian
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216154-plasmid-map-sequence-id-437765

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  6. GLP1R-DuET

    Plasmid
    #213253
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GLP1R (GLP1R Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213253-plasmid-map-sequence-id-430803

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  8. myc-BioID2-MCS

    Plasmid
    #74223
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    Myc and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    74223-plasmid-map-sequence-id-334595

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  9. TDP-REGv2(mScarlet-FLAG)#9 in pTwist-CMV backbone

    Plasmid
    #216153
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells (brighter than #7). It uses a single intron. [Code 'B11']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic splice site and C-terminal FLAG tag
    Expression
    Mammalian
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216153-plasmid-map-sequence-id-437757

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  10. pCAGGS-FuG-E

    Plasmid
    #67509
    Purpose
    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type E envelope coating virus particle with NeuRet.
    Depositor
    Kazuto Kobayashi
    Article
    Kato et al J Neurosci Methods. 2014 Apr 30;227:151-
    Insert
    Fusion Glycoprotein type E
    Use
    Lentiviral
    Tags
    Fusion protein of Vesicular stomatitis Indiana vi…
    Promoter
    CAGGS
    Available Since
    July 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67509-plasmid-map-sequence-id-118281

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  11. pB025

    Plasmid
    #183092
    Purpose
    Expresses FnCas12a in Bacteroides and used for genome editing
    Depositor
    Lei Dai
    Article
    Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47
    Insert
    FnCas12a, gRNA, Promoter, HAB, TetR,
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P1TDPGH023
    Available Since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183092-plasmid-map-sequence-id-368147

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Showing: 181 - 200 of 169885 results