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179,521 results

Showing: 4121 - 4160 of 179521 results

  1. pDG458

    Plasmid
    #100900
    Purpose
    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100900-plasmid-map-sequence-id-457690

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  5. DRD2-Tango

    Plasmid
    #66269
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DRD2 (DRD2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    66269-plasmid-map-sequence-id-157899

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  11. mCherry-Vimentin-N-18

    Plasmid
    #55158
    Purpose
    Localization: Intermediate Filaments, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Vimentin (VIM Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    55158-plasmid-map-sequence-id-267740

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  12. mApple-VE-Cadherin-N-10

    Plasmid
    #54959
    Purpose
    Localization: Tight Junctions, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VE-Cadherin (CDH5 Human)
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    I503T
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    54959-plasmid-map-sequence-id-209018

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  35. pDAC439

    Plasmid
    #195237
    Purpose
    Mammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)
    Depositor
    Jennifer Doudna
    Publication
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
    Expression
    Mammalian
    Available since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195237-plasmid-map-sequence-id-382945

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  36. pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #190033
    Purpose
    vector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promoters
    Depositor
    Huawei Tong
    Publication
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized hfCas13X
    Expression
    Mammalian
    Mutation
    Y672A,Y676A
    Promoter
    CBh, SV40, hU6
    Available since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    190033-plasmid-map-sequence-id-488812

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Showing: 4121 - 4160 of 179521 results