We narrowed to 31,527 results for: Eng
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Hiv7CMV-Cremyc-2A-GFP
Plasmid#117148PurposeExpress Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.DepositorInsertCremyc-2A-GFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHCas9-Nours
Plasmid#107733PurposeEscherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiaeDepositorInsertCas9
UseCRISPRTagsSV40 NLSExpressionYeastMutationHuman optimizedPromoterTEF1Available sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-Syk-tSH2
Plasmid#111269Purposeexpress murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJKW1813
Plasmid#174003PurposeExpresses PpCYP90G4 and AtATR1 for reconstitution of diosgenin biosynthesis in yeastDepositorInsertsUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Nxph4-6A-HA
Plasmid#128103PurposeExpress HA tagged Nxph4-6ADepositorInsertNxph4 (Nxph4 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUP95PDZGW (C5)
Plasmid#74021Purposelentiviral expression of Psd95-EGFP fusionDepositorInsertPsd95 (DLG4 Rat)
UseLentiviralTagsExpressionMammalianMutationPDZPromoterhUbCAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_10A
Plasmid#91207PurposeDirect Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: noneDepositorInsert35S:AtCas9 + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pITGFP89
Plasmid#83560PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.DepositorInsertTEF1 promoter -yEGFP-CLN2PEST
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
tetO7_Ef1a_OCT4
Plasmid#18918DepositorInsertOCT4 (POU5F1 Human)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop
Plasmid#169369PurposeExpression of gRNA in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop
UseCRISPRTagsExpressionInsectMutationPromoterCPIJ039653Available sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pLV-EF1a-V5-Vrk2
Plasmid#120240PurposeExpresses V5-tagged Vrk2 in lentiviral vectorDepositorInsertVrk2 (Vrk2 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVMG0146_Cq-U6-1_2xBbsI-gRNA
Plasmid#169238PurposeExpression of gRNA in Culex quinquefasciatus or other mosquitoesDepositorInsertCq-U6-1_2xBbsI-gRNA
UseCRISPRTagsExpressionInsectMutationPromoterCPIJ039653Available sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVMG0149_Cq-U6-6_2xBbsI-gRNA
Plasmid#169323PurposeExpression of gRNA in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0149_Cq-U6-6_2xBbsI-gRNA
UseCRISPRTagsExpressionInsectMutationPromoterCPIJ039596Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag-Sesn1b
Plasmid#61867PurposeTransient overexpression of Flag-Sesn1b (mouse)DepositorInsertSesn1 (Sesn1 Mouse)
UseTagsFlagExpressionMammalianMutationnonePromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEM-myr-eGFP-SV40pA-FKF
Plasmid#119866PurposeDonor cassette containing myr-tagged eGFP and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertmyr-tagged eGFP and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTags2x myristoylationExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-zDicer
Plasmid#171539PurposeEncodes Zebrafish Dicer proteinDepositorInsertdicer1(MK642699) (dicer1 Zebrafish)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM-myr-tdTomato-SV40pA-FKF
Plasmid#119867PurposeDonor cassette containing myr-tagged tdTomato and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertmyr-tagged tdTomato and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTags2x myristoylationExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
FHp5pUP95C3,5SGW (E5)
Plasmid#74035Purposelentiviral expression of Psd95 shRNA and Psd95-C3,5S-EGFP fusionDepositorInsertPsd95
UseLentiviral and RNAiTagsExpressionMammalianMutationPCR: C3,5S (tct aga cca cca tgg act Ctc tAt CGa t…PromoterH1Available sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP86
Plasmid#83543PurposeUsed to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.DepositorInsertRPL4A promoter-yEGFP
UseTagsExpressionYeastMutationPromoterRPL4AAvailable sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pInducer21 Flag-Sesn2
Plasmid#61829PurposeDox inducible lentivirial expression of Flag-Sesn2 (mouse)DepositorInsertSesn2 (Sesn2 Mouse)
UseTagsFlagExpressionMammalianMutationnonePromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop
Plasmid#169370PurposeExpression of gRNA in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop
UseCRISPRTagsExpressionInsectMutationPromoterCPIJ039596Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFP84
Plasmid#83542PurposeUsed to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.DepositorInsertSSA1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterSSA1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TU#1806_CRISPR_unc-73_exon21
Plasmid#82360Purposeto create unc-73E null alleleDepositorInsertCas9 and sgRNA against unc-73 exon21 (unc-73 Nematode)
UseCRISPRTagsExpressionWormMutationPromoterU6Available sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tol2-UAS-Dendra2-miR-223 sponge
Plasmid#97146PurposeFor miR-223 knock downDepositorInsertmiR-223 sponge
UseZebrafishTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
L1-1151Y>A
Plasmid#13269DepositorInsertL1CAM (L1CAM Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pInducer21 Flag-Sesn1b
Plasmid#61865PurposeDox inducible lentivirial expression of Flag-Sesn1b (mouse)DepositorInsertSesn1 (Sesn1 Mouse)
UseTagsFlagExpressionMammalianMutationnonePromoterAvailable sinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
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Tol2-krt4-RFP-miR-223 sponge
Plasmid#97150PurposeFor miR-223 knock downDepositorInsertmiR-223 sponge
UseZebrafishTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-human PUM1-HD PUF531_Q867E-Q939E-C935S-Q1011E- C1007S-intein-CBD
Plasmid#73306PurposeExpresses mutant human PUM1-HDDepositorInserthuman PUM1 Pumilio homology domain (PUM1 Human)
UseTagsinteinExpressionBacterialMutationQ867E-Q939E-C935S-Q1011E-C1007SPromoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFL-TK-AID3'UTR-MUT
Plasmid#19987DepositorInsertAID 3' UTR (Aicda Mouse)
UseTagsExpressionMammalianMutationMutationPromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pUASp-Flag-Myc-Qin-attB
Plasmid#44208DepositorInsertQin full length (qin Fly)
UseFly transformationTagsFlag and MycExpressionMutationPromoterAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHRIG-S6K
Plasmid#59446Purposelentiviral expression of S6K and EGFPDepositorInsertS6K (Rps6kb1 Rat)
UseLentiviralTagsIRES-EGFPExpressionMammalianMutationS6K starts at aa24 and contains 7 extra amino aci…PromoterCMVAvailable sinceOct. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT16
Plasmid#223388PurposeT-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter.DepositorInsertZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceMay 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG005
Plasmid#231309PurposeVector part for BEVA; BEVA2.0 Position 3 RSF1010 broad host range origin of replication with oriT, CmR.DepositorInsertBEVA2.0 Position 3 RSF1010 broad host range origin of replication with oriT, CmR.
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT15
Plasmid#223387PurposeT-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by 2x35s and the sgRNA was driven by AtU3 promoter.DepositorInsert2x35s-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT17
Plasmid#223389PurposeT-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter.DepositorInsertZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT23
Plasmid#223395PurposeT-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and sgRNA was driven by OsU3 promoter.DepositorInsertZmUbi-hA3A-Y130F-SpRYD10A-UGI-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT26
Plasmid#223398PurposeT-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by AtU3; BASTA for plants select.DepositorInsertZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT28
Plasmid#223400PurposeT-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and sgRNA was driven by OsU3; Hygromycin for plant select.DepositorInsertZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT29
Plasmid#223401PurposeT-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by OsU3; BASTA for plant select.DepositorInsertZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT30
Plasmid#223402PurposeT-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 and the sgRNA was driven by separate ZmUbi1; Hygromycin for plants selection.DepositorInsertZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-ZmUbi-gRNA scaffold 2.0-tRNA
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT14
Plasmid#223386PurposeT-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and sgRNA was by AtU3; BASTA for plant selection.DepositorInsertZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLS-CMV-mP-Rluc
Plasmid#213507PurposeCMV-driven Renilla luciferase expression vector for luciferase lentivirus-based reporter assayDepositorInsertLuciferase
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT221: pR6K-Donor(ZooTsy)_H1:12_E1:135_E2:20_H2:12
Plasmid#200874PurposeZooTsy donor harboring KanR cargo.DepositorInsertZooTsy
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT401: pHelper(AjTn6022)_QF-separated_TniQ-K361D
Plasmid#200861PurposeInducible expression of AjTn6022 proteins.DepositorInsertAjTn6022
UseTagsExpressionBacterialMutationTniQ-K361DPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT400: pHelper(AjTn6022)_QF-separated_TniQ-K348D
Plasmid#200860PurposeInducible expression of AjTn6022 proteins.DepositorInsertAjTn6022
UseTagsExpressionBacterialMutationTniQ-K348DPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMSIP103: pTarget(CyCAST)
Plasmid#200849PurposeTarget plasmid containing CyPSP1 and attachement site for CyCAST.DepositorInsertsPAM for CyCAST + CyPSP1
tRNA-Leu
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only
