We narrowed to 12,998 results for: cdna

1. pcDNA3.1-mas-KB2123-Nluc

   Plasmid

   #204325

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   KB2123-Nluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA 3.1(-) mouse Egr3

   Plasmid

   #107998

   Purpose

   Expresses mouse Egr3 in mammalian cells

   Depositor

   Peter Johnson

   Article

   Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

   Insert

   Egr3 (Egr3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA5FRT-EF-Pdgfralpha-EGFPN D842V

   Plasmid

   #66789

   Purpose

   Expression of constitutively active murine PDGFRalpha D842V tagged with GFP under the control of EF1a promoter

   Depositor

   Lotte Pedersen

   Article

   Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

   Insert

   PDGFRalpha (Pdgfra Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D842V (constitutively active)

   Promoter

   EF1a

   Available since

   July 14, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1(+)/Puro/TagRFP-LL5b

   Plasmid

   #112828

   Purpose

   transient/stable overexpression

   Depositor

   Joshua Sanes, Tomasz Prószyński

   Article

   Proszynski et al J Cell Sci. 2013 May 15;126(Pt 10):2225-

   Insert

   LL5b (Phldb2 Mouse)
   

   Use

   Tags

   TagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-FLAG-MTOR-E1799K

   Plasmid

   #69009

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-E1799K (MTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Glutamate 1799 to Lysine

   Promoter

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-dCas9-dMQ1-EGFP

   Plasmid

   #89637

   Purpose

   Targeted CpG methylation

   Depositor

   Margaret Goodell

   Article

   Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

   Insert

   site-specific DNA-methyltransferase SssI
   

   Use

   CRISPR

   Tags

   3*FLAG, 6*His, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   C141S, TGC-->TCC; S317A, AGC-->GCC

   Promoter

   CMV

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5-FRT/TO-N-mEGFP-PARP6

   Plasmid

   #178005

   Purpose

   Expresses N-terminally mEGFP-tagged PARP6

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   PARP6 (PARP6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA5-FRT/TO-N-mEGFP-PARP15

   Plasmid

   #178000

   Purpose

   Expresses N-terminally mEGFP-tagged PARP15

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   PARP15 (PARP15 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5-FRT/TO-N-mEGFP-PARP11

   Plasmid

   #177998

   Purpose

   Expresses N-terminally mEGFP-tagged PARP11

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   PARP11 (PARP11 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5/FRT EGFP-miniAID

    Plasmid

    #101714

    Purpose

    For amplification of a EGFP-miniAID for N-terminal tagging

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    EGFP-miniAID
    

    Use

    Tags

    EGFP-miniAID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5/FRT/TO GFP DNAJA1

    Plasmid

    #19491

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    DNAJA1 (DNAJA1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AIRAP 9: PSMD2.GST.V2.pcDNA3

    Plasmid

    #21799

    Depositor

    David Ron

    Article

    Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

    Insert

    PSMD2 (PSMD2 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-mNeonGreen-LEHD-NanoLuc

    Plasmid

    #98289

    Purpose

    caspase-9 BRET Sensor

    Depositor

    Maarten Merkx

    Article

    den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

    Insert

    mNeonGreen-LEHD-NanoLuc
    

    Use

    Tags

    His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA6-ARID1A 1759-2285

    Plasmid

    #39476

    Depositor

    Ie-Ming Shih

    Article

    Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

    Insert

    ARID1A (ARID1A Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    expresses ARID1A aa1759-2285

    Promoter

    Available since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-FLAG-MTOR-R2505P

    Plasmid

    #69015

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-R2505P (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Arginine 2505 to Proline

    Promoter

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1(+)-NES-ZapCV2 (cpV143)

    Plasmid

    #112060

    Purpose

    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)

    Depositor

    Amy Palmer

    Article

    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

    Insert

    NES-ZapCV2
    

    Use

    Tags

    Nuclear Export Signal (NES) derived from HIV-1

    Expression

    Mammalian

    Mutation

    ECFP gene is truncated 36 bp at 3' end. Venu…

    Promoter

    CMV

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-N-Myc_DED/AAA USP7

    Plasmid

    #131253

    Purpose

    Mammalian expression of an N-terminally Myc-tagged USP7 mutant, with mutations in UBL2 (DE758AA_D764A)

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Contains point mutations that convert USP7 aspart…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-StrepHA-SUMO2(Q87R)

    Plasmid

    #66868

    Purpose

    Mammalian expression of SUMO2(Q87R) with a strep and an HA tag

    Depositor

    Ying Liu

    Article

    Bursomanno et al J Proteome Res. 2015 Apr 3;14(4):1687-99

    Insert

    SUMO2 (SUMO2 Human)
    

    Use

    Tags

    HA and Strep

    Expression

    Mammalian

    Mutation

    Q87R

    Promoter

    CMV

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-HNF1B-WT-Flag-UTR

    Plasmid

    #183239

    Purpose

    Expression of FLAG tagged HNF1B

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    HNF1 homeobox B (HNF1B Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-Nuc-ExRai-AktAR2

    Plasmid

    #184049

    Purpose

    Nuclear-targeted ExRai-AktAR2.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    Nuc-ExRai-AktAR2
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA5-FRT/TO-Flag-RNF169

    Plasmid

    #74243

    Purpose

    Expresses RNF169 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Panier et al Mol Cell. 2012 Aug 10;47(3):383-95. doi:

    Insert

    RNF169 (RNF169 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pcDNA5/FRT/TO-GAlpha11-RLuc8

    Plasmid

    #140983

    Purpose

    Encodes a G alpha subunit (GNA11) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlpha11-RLuc8 (GNA11 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-Golgi-ExRai-AktAR2

    Plasmid

    #184051

    Purpose

    Golgi-targeted ExRai-AktAR2.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    Golgi-ExRai-AktAR2
    

    Use

    Tags

    N-terminal 35 amino acids from human endothelial …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-hAsCpf1(TYCV) (pY210)

    Plasmid

    #89351

    Purpose

    Expresses humanized AsCpf1 variant that recognizes TYCV PAMs

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1, RR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    S542R/K607R

    Promoter

    CMV

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA5-FRT/TO-FLAG-SHLD2

    Plasmid

    #114117

    Purpose

    Expresses N-terminally tagged FLAG-SHLD2 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD2 (SHLD2 Human)
    

    Use

    Genomic integration, tetracycline inducible

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO2

    Available since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pcDNA4-TO-Hygromycin-mVenus-MAP

    Plasmid

    #44099

    Depositor

    Dannel McCollum

    Article

    Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

    Type

    Empty backbone

    Use

    Tags

    mVenus-MAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA5/FRT/TO-FLAG-CHK1

    Plasmid

    #86875

    Purpose

    To express CHK1 with a FLAG tag at N-terminus.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    CHEK1 (CHEK1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA5 FRT-TO-EGFP-UPF1-WT

    Plasmid

    #136000

    Purpose

    DOX-inducible WT UPF1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    UPF1
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3-Casp7 C186A-Flag

    Plasmid

    #11816

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

    Insert

    Caspase 7 catalytic mutant (CASP7 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C186A

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

30. pcDNA3-eGFP-NLS-RNF168

    Plasmid

    #133977

    Purpose

    mammalian expression vector of eGFP tagged wild type RNF168

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    siRNA resistant sequence: GAGGAGTCGTGTTTATTGA

    Promoter

    CMV

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pcDNA3.1(+)-HLA-Flag-natT1R2

    Plasmid

    #113947

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from HLA class I …

    Expression

    Mammalian

    Mutation

    residues 22-839

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3-H2A-Akt-STOPS

    Plasmid

    #175010

    Purpose

    Akt-STOPS targeted to the nucleus via histone H2A.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    H2A-mCherry-Akt-STOPS
    

    Use

    Tags

    Histone H2A and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA-c/o NL4-3 Env delta CT

    Plasmid

    #139285

    Purpose

    HIV-1 NL4-3 glycoprotein lacking cytoplasmic tail

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    codon optimized HIV-1 Env delta cytoplasmic tail
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of cytoplasmic tail after residue 710

    Promoter

    CMV IE

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA4-Beclin1 243-450

    Plasmid

    #24392

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains amino acids 243-450.

    Promoter

    Available since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

35. pcDNA4-Beclin1 151-241

    Plasmid

    #24390

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains amino acids 151-241.

    Promoter

    Available since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.1(-) MID51 4xMyc Hisx6

    Plasmid

    #44598

    Depositor

    David Chan

    Article

    Loson et al Mol Biol Cell. 2013 Jan 2.

    Insert

    MiD51 (Mief1 Mouse)
    

    Use

    Tags

    4xMyc and 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-K9 histone methylation reporter

    Plasmid

    #22866

    Depositor

    Alice Ting

    Article

    Lin et al J Am Chem Soc. 2004 May 19. 126(19):5982

    Insert

    K9 HMR
    

    Use

    Tags

    CFP and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

38. pcDNAintron-CHIKV-nsp3-c3xFLAG

    Plasmid

    #215696

    Purpose

    Produces the Chikungunya virus nonstructural protein 3 with a c-terminal 3xFLAG tag

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV nsp3
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pcDNA5 FRT TO Myc ArA WT

    Plasmid

    #59804

    Purpose

    Allows the integration of Myc ArA in the genome and Tet-inducible expression

    Depositor

    Stephen Taylor

    Article

    Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

    Insert

    Aurora A (AURKA Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA/Myc DeltaN TCF4

    Plasmid

    #16513

    Depositor

    Bert Vogelstein

    Article

    Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

    Insert

    TCF4 (TCF7L2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Delta N: lacks aa 1-32

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only