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159,589 results

Showing: 5351 - 5400 of 159589 results

  4. TFORF3018

    Plasmid
    #144494
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NEUROG3 (NEUROG3 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    144494-plasmid-map-sequence-id-392724

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  5. FSW_jGCaMP8f

    Plasmid
    #197034
    Purpose
    Express GCaMP8f from hSyn1 promoter
    Depositor
    Ronald Hart
    Insert
    GCaMP8f
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197034-plasmid-map-sequence-id-392710

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  8. PB-CT3G-CERP2-MG-IRES2-mTagBFP2

    Plasmid
    #175509
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175509-plasmid-map-sequence-id-345099

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  9. pET StrepII TEV LIC cloning vector (1R)

    Plasmid
    #29664
    Depositor
    Scott Gradia
    Type
    Empty backbone
    Use
    Tags
    Strep II and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    29664-plasmid-map-sequence-id-13779

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  11. ND4.2-Left TALE-G1397-N-DddA11-mCherry

    Plasmid
    #179682
    Purpose
    Expression of base editor in mammalian cells and for cell sorting
    Depositor
    David Liu
    Article
    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387
    Insert
    COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_P2A_mCherry_ATP5B 3'UTR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S1330I A1341V N1342S E1370K T1380I
    Promoter
    Available since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    179682-plasmid-map-sequence-id-355098

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  17. pHdzCas9-KRAB

    Plasmid
    #92341
    Purpose
    Expression dzCas9-KRAB in microalgae. CRISPR/Cas based plant genome editing and gene regulation, Hyg resistance
    Depositor
    I-Son Ng
    Article
    Kao et al Bioresour Technol. 2017 May 4. pii: S096
    Type
    Empty backbone
    Use
    CRISPR; Plant expression
    Tags
    KRAB
    Expression
    Plant
    Mutation
    Promoter
    CaMV35S
    Available since
    June 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    92341-plasmid-map-sequence-id-181654

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  22. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

    Plasmid
    #217635
    Purpose
    AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.
    Depositor
    Martin Kampmann
    Insert
    mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    EF1alpha and mU6
    Available since
    Oct. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    217635-plasmid-map-sequence-id-449624

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  28. APEX2-OMM

    Plasmid
    #79056
    Purpose
    Soybean APEX2 anchored to cytosolic face of outer mitochondrial membrane with C-terminal targeting sequence of last 31aa of human mitochondrial antiviral-signaling protein
    Depositor
    Alice Ting
    Article
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Insert
    Soybean APEX2
    Use
    Lentiviral
    Tags
    flag and last 31 amino acids of human MAVS
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV immearly promotor
    Available since
    Aug. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    79056-plasmid-map-sequence-id-238450

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  30. pCS2+MCS-P2A-sfGFP

    Plasmid
    #74668
    Purpose
    Plasmid for expression of wild-type or mutant cDNA fragments (mutation reporter) or complete cDNAs in frame with superfolderGFP.
    Depositor
    Jason Berman
    Insert
    sfGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    74668-plasmid-map-sequence-id-146800

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  31. pRJPaph-bjGFP

    Plasmid
    #67032
    Purpose
    Plasmid for Paph-bjGFP integration downstream of B. diazoefficiens (B. japonicum) scoI
    Depositor
    Julia Vorholt
    Article
    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.
    Inserts
    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    bjGFP
    Use
    Conjugative bacterial vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67032-plasmid-map-sequence-id-115543

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  33. pOPS0379

    Plasmid
    #115510
    Purpose
    Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing sfGFP
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Rlv3841pNifH(pOGG082) sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115510-plasmid-map-sequence-id-224287

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  34. pCMV-MbPylRS(DiZPK)

    Plasmid
    #91706
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest
    Depositor
    P. Chen
    Article
    Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5
    Inserts
    pylS
    pylT
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A
    Promoter
    Available since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91706-plasmid-map-sequence-id-183279

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  38. pRL_GI_PCB28

    Plasmid
    #98745
    Purpose
    For phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919
    Inserts
    heme oxygenase 1 (TED4 Mustard Weed)
    Phycocyanobilin:ferredoxin oxidoreductase
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    ADH1m and PGK1
    Available since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98745-plasmid-map-sequence-id-189854

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  42. pOPS0381

    Plasmid
    #133231
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133231-plasmid-map-sequence-id-282538

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  43. pCS2+ heiCas9

    Plasmid
    #186929
    Purpose
    For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS
    Depositor
    Joachim Wittbrodt
    Article
    Thumberger et al Elife. 2022 Mar 25;11. pii: 70558. doi:
    Insert
    myc-flexible linker-oNLS-Cas9-oNLS
    Use
    CRISPR
    Tags
    myc-flexible linker-optimizedNLS and optimizedNLS
    Expression
    Mammalian
    Mutation
    Promoter
    sp6
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    186929-plasmid-map-sequence-id-390733

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  47. pET28a-HsSENP1

    Plasmid
    #71465
    Purpose
    Encodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 1 (SENP1 Human)
    Use
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  49. pRRlsinPGK_BFP2_WPRE

    Plasmid
    #91787
    Purpose
    Constitutive expression of mTagBFP2 using PGK promoter in a lentiviral backbone.
    Depositor
    Beverly Torok-Storb
    Insert
    mTagBFP2
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    91787-plasmid-map-sequence-id-180618

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  50. plex.mcs sv40promoter-luc

    Plasmid
    #129429
    Purpose
    Luc expression under SV40 promoter
    Depositor
    Oskar Laur
    Insert
    LUC
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129429-plasmid-map-sequence-id-256107

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Showing: 5351 - 5400 of 159589 results