-
Plasmid#170990PurposeFor bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-R9-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-pHD118-GFP-HiBit
Plasmid#171015PurposeFor bacterial expression of bdSUMO tagged muGFP with pHD118 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-pHD118-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Empty Anchor Control
Plasmid#127665PurposeEncodes for puromycin resistance expressed from the EF1a promoterDepositorInsertPuro
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
p416GAL1-Zipp.-L-IFP-F[1] (Yeast)
Plasmid#52895PurposePlasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[1]
UseTagsL-IFP-F[1]ExpressionYeastMutationPromoterGAL1Available sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
Plasmid#173866PurposeMammalian expression of mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)DepositorInsertmVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjFOXL2-coemiRFP670-PGK-Puro
Plasmid#186168PurposeKnock-in gene targeting in marmoset cells at FOXL2 locusDepositorInsertemiRFP670
UseMarmoset targetingTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
Gata3-MIEG-hCD4
Plasmid#40568DepositorInsertGata3 (Gata3 Mouse)
UseRetroviralTagsIRES hCD4ExpressionMutationPromoterAvailable sinceMarch 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7g1
Plasmid#14624DepositorInsertMKK7g1 (Map2k7 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
H2B-2A-GFP-IRESpuro2
Plasmid#183746PurposeExpresses H2B-2A-GFPDepositorInsertH2B (H2BC16P Human)
UseTagsGFP with 2A protease cleavage site between H2B an…ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
HGXPRT
Plasmid#64201PurposeExpression of Plasmodium falciparum HGXPRTDepositorInsertHypoxanthine guanine xanthine phosphoribosyltransferase
UseTagsHistidine tagExpressionBacterialMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15A-tac- YFP
Plasmid#186570Purposep15A ori, lacI, Ptac promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsExpressionBacterialMutationPromotertac promoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSPYngK-hp
Plasmid#48118PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoterDepositorInsertsignal peptide of YngK
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-mNeurog2 gRNA_1-MS2-Puro
Plasmid#192680PurposeLentiviral expression of sgRNA targeting mIL1RN promoter to activate mouse Neurog2 transcriptionDepositorInsertMouse Neurog2 activating gRNA #1 (Neurog2 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P32
Plasmid#120043PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with S-TagDepositorInsertS-Tag
UsePcr templateTagsS-TagExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
1352wUV2 F3bbs kan NNNTGGGCGk
Plasmid#18756DepositorInsert3 tandem zinc fingers
UseTagsExpressionBacterialMutationA Kan cassette (1090bp) is inserted between two b…PromoterAvailable sinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMT-GFP-p150Dynactin
Plasmid#24283DepositorInsertp150 Dynactin (DCTN1-p150 Fly)
UseTagsGFPExpressionInsectMutationPromoterAvailable sinceJune 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
FKBP-YPet-RanGAP*
Plasmid#175245PurposeBacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPetDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationAmino acid 562 F to APromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
CyPet-FKBP-E2
Plasmid#175244PurposeBacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycinDepositorInsertUBE2I (UBE2I Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
1352wUV2 F1bbs kan GCGTGGNNN
Plasmid#18753DepositorInsert3 tandem zinc fingers
UseTagsExpressionBacterialMutationA Kan cassette (1090bp) is inserted between two b…PromoterAvailable sinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-EBFP2
Plasmid#184939PurposeER Visualization (OSER assay) Expresses EBFP2 in fusion with N-terminal part of p450DepositorInsertCytERM-EBFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-PTEN D92A
Plasmid#50521PurposeThis plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertPTEN (PTEN Human)
UseTagsGFPExpressionMammalianMutationD92APromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z25
Plasmid#120068PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with YFP-LIDDepositorInsertYFP-LID
UsePcr templateTagsYFP-LIDExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
SGL40C.EFS.RFP657
Plasmid#69147PurposeAdvanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with RFP657 expression (EFS Promoter)DepositorInsertssgRNA
EFS
tagRFP657
UseTagsExpressionMutationPromoterAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab4 DN
Plasmid#37691DepositorInsertRab4 (Rab4 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS22N (dominant negative)PromoterGAL4Available sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNTPTP alpha (S180A/S204A)
Plasmid#17700DepositorInsertPTPalpha S180A S204A (PTPRA Human)
UseTagsExpressionMammalianMutationS180A / S204APromoterAvailable sinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P19
Plasmid#124789PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry-mNeonGreenDepositorInsertmCherry-mNeonGreen
UsePcr templateTagsmCherry-mNeonGreenExpressionMutationPromoterNoneAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
2674 pBS Bcl-2 P1+P2
Plasmid#8746DepositorInsertBcl-2 P1+P2 (BCL2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCsy-crRNA-EV
Plasmid#153944PurposeFor cloning and expression of Type I-F P. aeruginosa (PaeCascade) crRNA in mammalian cellsDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPyCAG-NE-EGFP-IRES-Pac
Plasmid#183617PurposeMammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered EGFP (NLS-EGFP-EmerinTMD). Confers puromycin resistance.DepositorInsertEGFP
UseTagsHuman EMD transmembrane domain and NLSExpressionMammalianMutationPromoterCAGAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3XFlag-CMV10-Flag-mMcl-1 KR
Plasmid#32979DepositorInsertMcl-1 (Mcl1 Mouse)
UseTags3XFlagExpressionMammalianMutationAll lys changed to ArgPromoterCMVAvailable sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z29
Plasmid#120072PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Strep-IIDepositorInsertStrep-II
UsePcr templateTagsStrep-IIExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBBK75 pHomL-RPL18Bp-Scarlet(rfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR)
Plasmid#179059PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-2xPH(PLCd), a marker for the plasma membraneDepositorInsertRPL18Bp-Scarlet(rfp)-Link-2xPH(PLCd)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT sfGFP 150TAG
Plasmid#154771Purposeplasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAGPromoterEF1Available sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-AtAFB2-mTagBFP2
Plasmid#179889PurposeExpresses AtAFB2 (auxin-inducible degron system) fused to mTagBFP2 (lentiviral plasmid)DepositorInsertAtAFB2 (AFB2 Synthetic, Mustard Weed)
UseLentiviralTagsmTagBFP2ExpressionBacterial and MammalianMutationPromoterEF1Available sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
p413GAL1-Zipp.-L-IFP-F[2] (Yeast)
Plasmid#52893PurposePlasmid+insert encoding GCN4-leucine zipper fused to IFP-F[2] by the linker (GGGGS) X 2. Zipper-L-IFP-F[2] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[2]
UseTagsL-IFP-F[2]ExpressionYeastMutationPromoterGAL1Available sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL4.13/GUG-FFLuc-3XFLAG
Plasmid#127334PurposeExpress 3xFLAG tagged firefly luciferase (FFLuc) from GUG start codonDepositorInsertFirefly luciferase
UseTags3xFLAGExpressionMammalianMutationPromoterSV40Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(rhesus)
Plasmid#79033PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(rhesus)
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterpolyhedrinAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-CAML-Myc-His
Plasmid#22295DepositorInsertcalcium modulating ligand (CAMLG Human)
UseTagsMyc-HisExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-zDicer
Plasmid#171539PurposeEncodes Zebrafish Dicer proteinDepositorInsertdicer1(MK642699) (dicer1 Zebrafish)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL2-Skp2 promoter-luciferase
Plasmid#81119PurposeReporter activity of SKP2 gene promoterDepositorInsertSKP2 (Skp2 Mouse)
UseLuciferaseTagsluciferaseExpressionMutationcontains the region from 1_kb upstream of transcr…PromoterSKP2Available sinceSept. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
SEPT11 sh1 shRNA
Plasmid#122020PurposeKnock Down of Septin 11. It targetes Septin 11 mRNA. It knocks down all known splice variants of Sept 11 mRNAs.DepositorInsertSEPT11 shRNA (Sept11 Rat)
UseRNAiTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-164
Plasmid#118544PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmOrange2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-pHlip-GFP-HiBit
Plasmid#171016PurposeFor bacterial expression of bdSUMO tagged muGFP with pHlip peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-pHlip-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-166
Plasmid#118546PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmCardinal
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRI007-pGEM-PgpdA-Cas12aScaffold-BsmbI-TtrpC
Plasmid#140200PurposeCloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsdLbCas12a crRNA scaffoldExpressionMutationPromoterPgpdAAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15a-PLtetO-1-UTR1-LacI-T500
Plasmid#45784DepositorInsertLac repressor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLtetO-1Available sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-Vinculin-23
Plasmid#57978PurposeLocalization: Focal Adhesions, Excitation: 549, Emission: 565DepositorInsertVinculin (VCL Human)
UseTagsmOrange2ExpressionMammalianMutationV234L in vinculinPromoterCMVAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
5mCUC1 pGreenII0129
Plasmid#12067DepositorInsertCUC1 mutant (CUC1 Mustard Weed)
UsePlant transformation via agrobacteriumTagsExpressionMutationmiR-resistant CUC1. Made by introducing five sile…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLpC-Zipp.-L-IFP-F[2]+Venus (mammalian)
Plasmid#52906PurposeBinary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[2]+Venus
UseRetroviralTagsL-IFP-F[2]ExpressionMammalianMutationPromoterUnknownAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO Plk4 KD ∆24-mCherry
Plasmid#80272Purposemammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deletedDepositorInsertPlk4 (PLK4 Human)
UseTagsmCherryExpressionMammalianMutationdeletion of multi-phospho degron (amino acids 282…PromoterCMVAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only