We narrowed to 2,798 results for: ALC

1. ER-G-Ca-FLITS-KDEL

   Plasmid

   #191464

   Purpose

   Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   G-Ca-FLITS
   

   Use

   Tags

   ER and KDEL

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. sspa-GCaMP6s

   Plasmid

   #67559

   Purpose

   short photoactivatable fluorescent calcium reporter

   Depositor

   Ehud Isacoff

   Article

   Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

   Insert

   spa-GCaMP6s
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

   Promoter

   Available since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pRSET-triple mutation-pa-GCaMP6f

   Plasmid

   #67570

   Purpose

   bacterial expression of a photoactivatable fluorescent calcium reporter

   Depositor

   Ehud Isacoff

   Article

   Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

   Insert

   pa-GCaMP6f
   

   Use

   Tags

   HIS-tag

   Expression

   Bacterial

   Mutation

   V115H, L64F, T65S

   Promoter

   T7

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA5/FRT/TO/intron/FlagCter

   Plasmid

   #113548

   Purpose

   Mammalian expression plasmid that can be used with the Flp-In T-REx system.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   Flag
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAV-Y-GECO1

   Plasmid

   #55767

   Purpose

   Express Y-GECO1 in neurons

   Depositor

   Robert Campbell

   Article

   Zhao et al Integr Biol (Camb). 2014 May 19.

   Insert

   Y-GECO1.0
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p104Tol2-tp1:Cas9-t2A-GFP, 4xU6:sgRNA

   Plasmid

   #227773

   Purpose

   Expression of tp1 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs in zebrafish

   Depositor

   Bushra Raj

   Article

   Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

   Inserts

   Cas9-t2A-GFP
   4xU6:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6 and tp1

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p155ME-loxP-dsR-loxP-Cas9-t2A-GFP

   Plasmid

   #227774

   Purpose

   Middle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloning

   Depositor

   Bushra Raj

   Article

   Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

   Insert

   loxP-dsR-loxP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GreenT-EC - pRSET

   Plasmid

   #193947

   Purpose

   Bacterial expression vector for a low-affinity calcium indicator

   Depositor

   Oliver Griesbeck

   Article

   Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

   Insert

   GreenT-EC
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GreenT-EC.b - pRSET

   Plasmid

   #193948

   Purpose

   Bacterial expression vector for a low-affinity calcium indicator

   Depositor

   Oliver Griesbeck

   Article

   Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

   Insert

   GreenT-EC.b
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GreenT-EC.c - pRSET

    Plasmid

    #193949

    Purpose

    Bacterial expression vector for a low-affinity calcium indicator

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC.c
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFPO-His-G-Ca-FLITS

    Plasmid

    #191455

    Purpose

    Bacterial isolation of genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    G-Ca-FLITS
    

    Use

    Tags

    His

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CeNL(Ca2+)_110μ-pcDNA3

    Plasmid

    #111929

    Purpose

    Cyan color bright luminescent calcium indicator with low affinity

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_110μ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E31D, F92W, E104D. D133E at CaM

    Promoter

    CMV

    Available since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGP-CMV-NES-fRCaMP1

    Plasmid

    #125249

    Purpose

    Expresses red fluorescent calcium sensor in mammalian cells

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRCaMp1
    

    Use

    Tags

    NES signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKL001

    Plasmid

    #98917

    Purpose

    Constitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Insert

    CaPR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23118 - Strong Constitutive

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LAMP1-mGFP

    Plasmid

    #34831

    Depositor

    Esteban Dell'Angelica

    Article

    Falcon-Perez et al J Cell Sci. 2005 Nov 15;118(Pt 22):5243-

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Tags

    GSTGSTGSTGA linker, Kozak, and mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA3.1(+)-NEMOm

    Plasmid

    #189930

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOs
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA3.1(+)-NEMOc

    Plasmid

    #189933

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGP-CMV-NEW-fRGECO2

    Plasmid

    #125248

    Purpose

    Expresses red fluorescent calcium sensor in mammalian cells

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRGECO2
    

    Use

    Tags

    NES signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-synapsin-HaloCaMP1a-EGFP

    Plasmid

    #138327

    Purpose

    chemigenetic calcium indicator

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Has Service

    AAV1 and AAV9

    Insert

    HaloCaMP1a
    

    Use

    AAV

    Tags

    EGFP, His-tag, and Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-NFAT luciferase

    Plasmid

    #17870

    Purpose

    3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue

    Depositor

    Jerry Crabtree

    Article

    Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.

    Insert

    3x NFAT binding sequence (IL2 )
    

    Use

    Luciferase

    Tags

    IL2 promoter and Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    IL-2 promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDNA3.1(+)-NEMOs

    Plasmid

    #189931

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOs
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDNA3.1(+)-NEMOf

    Plasmid

    #189932

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Salsa6f

    Plasmid

    #140188

    Purpose

    Salsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)

    Depositor

    Michael Cahalan

    Article

    Dong et al eLife. 2017 Dec 14;6. pii: 32417. doi: 1

    Insert

    Salsa6f
    

    Use

    Tags

    tdTomato-v5-GCaMP6f

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Immediate Early

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDNA3.1(+)-NEMOb

    Plasmid

    #189934

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOb
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGP-CMV-NES-fRCaMP2

    Plasmid

    #125250

    Purpose

    Expresses red fluorescent calcium sensor in mammalian cells

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRCaMP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D396A

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. piGECI-N1

    Plasmid

    #160421

    Purpose

    Cytoplasmic expression of a near-infrared genetically encoded calcium indicator iGECI

    Depositor

    Vladislav Verkhusha

    Article

    Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

    Insert

    iGECI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-synapsin-HaloCaMP1b-EGFP

    Plasmid

    #138328

    Purpose

    chemigenetic calcium indicator

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Has Service

    AAV1 and AAV9

    Insert

    HaloCaMP1b
    

    Use

    AAV

    Tags

    EGFP, His-tag, and Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJMK074: CMV QuasAr2-TS-GCaMP6f

    Plasmid

    #72303

    Purpose

    Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator

    Depositor

    Adam Cohen

    Article

    Dempsey et al J Pharmacol Toxicol Methods. 2016 May 13

    Insert

    CaViar
    

    Use

    Lentiviral

    Tags

    TS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Opto-CRAC version 1

    Plasmid

    #101245

    Purpose

    Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling

    Depositor

    Yubin Zhou

    Article

    He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1

    Insert

    LOV2 and STIM1 cytosolic fragment
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV.hSyn.CaMPARI.WPRE.SV40

    Plasmid

    #100832

    Purpose

    AAV expression of CaMPARI, a photoconvertible fluorescent protein-based calcium integrator

    Depositor

    Eric Schreiter, Loren Looger

    Article

    Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    CaMPARI
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pFR-mScarletI-antiFRET-P2A-G-Ca-FLITS

    Plasmid

    #191457

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) G-Ca-FLITS to mScarlet-I fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    G-Ca-FLITS
    

    Use

    Tags

    His-mScarletI-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. GreenT-EC - Display - CyRFP1

    Plasmid

    #193943

    Purpose

    Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC
    

    Use

    Tags

    Igk_secretion - HA-Flag and PDGFR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. G-GECO1-Orai3

    Plasmid

    #73563

    Purpose

    Green fluorescent reporter of Orai3-associated calcium influx

    Depositor

    Michael Cahalan

    Article

    Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

    Insert

    G-GECO1-Orai3 (ORAI3 Human, Synthetic)
    

    Use

    Tags

    G-GECO1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Immdeiate Early

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. GreenT-EC.b - Display - CyRFP1

    Plasmid

    #193944

    Purpose

    Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC.b
    

    Use

    Tags

    Igk_secretion - HA-Flag and PDGFR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. GreenT-EC.c - Display - CyRFP1

    Plasmid

    #193945

    Purpose

    Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC.c
    

    Use

    Tags

    Igk_secretion - HA-Flag and PDGFR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3-ER-CaNAR2

    Plasmid

    #64732

    Purpose

    Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of ER membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CYP450-CaNAR2
    

    Use

    Tags

    Cerulean3, N-terminal 27 amino acids from CYP450,…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCI-syn-fRGECO2

    Plasmid

    #125244

    Purpose

    Expresses red fluorescent calcium sensor in hippocampal slices

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRGECO2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W44Y, D432A

    Promoter

    synapsin

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. 1127-DP NTP wild type

    Plasmid

    #32398

    Depositor

    Kathleen Green

    Article

    Kowalczyk et al J Cell Biol. 1997 Nov 3;139(3):773-84.

    Insert

    Desmoplakin (DSP Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains only the first 584 aa

    Promoter

    CMV

    Available since

    Nov. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

39. pcDNA3-PM-CaNAR2

    Plasmid

    #64730

    Purpose

    Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    Lyn-CaNAR2
    

    Use

    Tags

    Cerulean3, N-terminal targeting sequence from Lyn…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV-GCaMP3bright

    Plasmid

    #74423

    Purpose

    Express fluorescent calcium indicator GCaMP3bright in mammalian cells

    Depositor

    Katalin Torok

    Article

    Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

    Insert

    GCaMP3bright
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D392A

    Promoter

    CMV

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCMV-GCaMP3fast

    Plasmid

    #74422

    Purpose

    Express fluorescent calcium indicator GCaMP3fast in mammalian cells

    Depositor

    Katalin Torok

    Article

    Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

    Insert

    GCaMP3fast
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W4Y D356A

    Promoter

    CMV

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3-Mito-CaNAR2

    Plasmid

    #64731

    Purpose

    Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNAR2
    

    Use

    Tags

    Cerulean3, N-terminal 30 amino acids from DAKAP1,…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pENN.AAV.CAG.Flex.CaMPARI.WPRE.SV40

    Plasmid

    #100831

    Purpose

    AAV expression of CaMPARI, a photoconvertible fluorescent protein-based calcium integrator

    Depositor

    Eric Schreiter, Loren Looger

    Article

    Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

    Insert

    CaMPARI
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCI-syn-fRCaMP2

    Plasmid

    #125246

    Purpose

    Expresses red fluorescent calcium sensor in hippocampal slices

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRCaMP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D396A

    Promoter

    synapsin

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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45. pMJA089

    Plasmid

    #128518

    Purpose

    Expresses human Lysozyme in Pichia pastoris

    Depositor

    Marcos Alcocer

    Article

    Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

    Insert

    Artificial gene for human lysozyme (LYZ Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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46. pcDNA3.1(+)-Mito-CaNARi

    Plasmid

    #64851

    Purpose

    Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNARi
    

    Use

    Tags

    Cerulean, N-terminal 30 amino acids from DAKAP1, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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47. piGECI-NES-N1

    Plasmid

    #160422

    Purpose

    Expression of a near-infrared genetically encoded calcium indicator iGECI with a nuclear export signal sequence

    Depositor

    Vladislav Verkhusha

    Article

    Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

    Insert

    iGECI-NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. pFR-mTq2-antiFRET-P2A-jRCaMP1b

    Plasmid

    #191468

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) jRCaMP1b to mTurquoise2 fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    jRCaMP1b
    

    Use

    Tags

    His-mTurquoise2-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pGP-CMV-NES-fRGECO1

    Plasmid

    #125247

    Purpose

    Expresses red fluorescent calcium sensor in mammalian cells

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRGECO2
    

    Use

    Tags

    NES signal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. p35S::mCh-HA-P2A-lifeact-mCh

    Plasmid

    #135234

    Purpose

    constitutive expression of 2 mCherry molecules: one tagged with HA and another that binds to actin (control plasmid)

    Depositor

    Armin Djamei

    Article

    Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

    Insert

    mCh-HA-P2A-lifeact-mCh
    

    Use

    Tags

    HA, lifeact, and p2a

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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