We narrowed to 9,280 results for: Pol
-
Plasmid#186435PurposeExpresses mCherry Cenexin in mammalian cells and zebrafish embryosDepositorAvailable SinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only
-
pCLXEBR-pTF-Ngn3
Plasmid#114307PurposeTET-inducible expression of the Neugenin 3 transcription factorDepositorInsertNgn3 (NEUROG3 Human)
UseLentiviralAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tom20-ECFP-FRB
Plasmid#210289Purposemitochondrial OM marker and "anchor" in FKBP-FRB CIDDepositorInsertTom20-ECFP-FRB
TagsECFP and FRBExpressionMammalianPromoterCMVAvailable SinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221075PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
ExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti.CMV/TO_PRKAA1
Plasmid#74446PurposeLentiviral expression vector encoding untagged WT human AMPK alpha 1DepositorAvailable SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSR646
Plasmid#65215PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-6 cap
UseAAVExpressionInsectPromoterP10 and polyhedrinAvailable SinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINS-USP21 (USP, aa 196-565)
Plasmid#61585PurposeExpresses human USP21 (USP domain) in E. coli.DepositorInsertUSP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)
TagsHis6-SUMOExpressionBacterialMutationDeleted aa 1-195.PromoterT7Available SinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
peGFP C1-SPG20 SC domain
Plasmid#218580PurposeExpresses GFP-tagged SC domain of human SPG20 in mammalian cellsDepositorInsertSPG20 (SPART Human)
TagseGFPExpressionMammalianMutationdeleted amino acids 1-417PromoterCMVAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBa. BDNF-Halo
Plasmid#162718PurposeMammalian expression of rat BDNFDepositorInsertBrain derived neurotrophic factor (Bdnf Rat)
TagsHaloExpressionMammalianMutationnonePromoterChicken Beta actinAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-eGFP-Cre
Plasmid#120219PurposeExpresses cre under the control of CamKIIa promotorDepositorInsertcre
UseAAV and Cre/LoxTagsEGFPAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pCMV-SEMPER_GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221076PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertGGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
ExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221077PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertCCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
ExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221079PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertTTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
ExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-mOC-STAMP-GFP
Plasmid#73579PurposeLentivirus expression of mOC-STAMPDepositorAvailable SinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmRFP-N1 human cofilin S3A
Plasmid#50857Purposeexpresses constituvely active cofilin fused to mRFP in mammalian cellsDepositorInsertcofilin 1 S3A (CFL1 Human)
TagsmRFPExpressionMammalianMutationS3A constitutively activePromoterCMVAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIRES-Puro-MGMT
Plasmid#187654PurposeExpresses hMGMT together with the puromycin resistance gene via an IRES.DepositorAvailable SinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCA12-pHR-SFFV-BFP-B23
Plasmid#199443PurposeExpression of BFP-B23 in mammalian cellsDepositorAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-FLInChR-mVenus
Plasmid#119298PurposeFLinChR is the fusion signal sequence and transmembrane (TM) domain of Neurexin 1B-delta to ChR2E123T/T159C for inversion ChR2 to an optogenetic inhibitorDepositorInsertNx1BTM-FCS-ChR2ET/TC-mVenus
UseAAVTagsmVenusPromoterCAGAvailable SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-KDM4A
Plasmid#188233PurposeExpress human KDM4A in mammalian cellsDepositorAvailable SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV BARK D110A p2A mCherry
Plasmid#117690PurposeCMV-iBARK(D110A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencingDepositorInsertBARKrgs D110A peptide
TagsHA / FLAGExpressionMammalianMutationD110AAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry-FKBP-GEF(TIAM1)
Plasmid#85156Purposeacute activation of Rac through rapamycin-induced translocation to membrane-localized FRBDepositorInsertFusion of mCherry, FKBP, and the DH-domain of human TIAM1 (amino acids 1012-1250)
TagsmCherry-FKBPExpressionMammalianPromoterCAGAvailable SinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSR660
Plasmid#65216PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-8 cap
UseAAVExpressionInsectPromoterP10 and polyhedrinAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hTGM2-V5
Plasmid#180413Purposelentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal V5 tag) geneDepositorAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 human cofilin S3E
Plasmid#50855Purposeexpresses psuedo-phosporylated, non-activatable cofilin in mammalian cellsDepositorInsertcofilin 1 S3E (CFL1 Human)
ExpressionMammalianMutationS3E psuedo-phosporylated, non-activatablePromoterCMVAvailable SinceFeb. 5, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
TRE-STOP EF1a-rtTA3
Plasmid#195882Purposecontrol construct of TRE-VEGFA EF1a-rtTA3DepositorAvailable SinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
cASPG1-mRFP
Plasmid#181962PurposeASPG1 protein tagged with mRFP, under control of TBA2 promoterDepositorAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-wt
Plasmid#60728PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.DepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…Available SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQHA-USP11 WT puroR
Plasmid#46749PurposeRetroviral vector that expresses wild type HA-tagged wild type human USP11DepositorAvailable SinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
LLP469 pEF1a-mCherry-EMPTY-gRNA
Plasmid#100958PurposemCherry driven by EF1a, empty gRNA scaffold driven by U6DepositorInsertgRNA empty backbone with RFP
UseCRISPRTagsRFPExpressionMammalianAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_Ubiquitin
Plasmid#131258PurposeMammalian expression of ubiquitin with an N-terminal HA tagDepositorAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSR651
Plasmid#65213PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-1 cap
UseAAVExpressionInsectPromoterp10 and polyhedrinAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
RDX-mCherry
Plasmid#214476PurposePlasmid for transient transfection of RDX-mCherry fusion protein in mammalian cells.DepositorInsertRDX (RDX Human)
TagsmCherryExpressionMammalianMutationSequence matches cDNA clone MGC:48283 IMAGE:52844…PromoterCMVAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQHA-USP11 CS puroR
Plasmid#46750PurposeRetroviral vector that expresses catalytically inactive form of HA-tagged human USP11DepositorInsertUSP11 (USP11 Human)
UseRetroviralTagsHAExpressionMammalianMutationC318S--catalytically inactivePromoterCMVAvailable SinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-OT (wt TCF-4 sites)
Plasmid#16558DepositorInsertTCF-4 binding sites (TCF4 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutation3 copies of wildtype Tcf-4 binding sitesAvailable SinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET-20b-M-Ntx-S1
Plasmid#58716PurposeExpresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1SDepositorInsertNotexin
TagsMethionineExpressionBacterialMutationChanged Asparagine 1 to SerinePromoterT7Available SinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SYN-H-sDarken
Plasmid#184797PurposeHigh affinity version of the Serotonin Sensor sDarken under the control of a hSyn Promoter.DepositorInsertH-sDarken
UseAAVExpressionMammalianPromoterhSynAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1-eIF4E K119A
Plasmid#199440PurposeBacterial expression plasmids for production of recombinant GST-eIF4E K119ADepositorInsert1977 (EIF4E Human)
TagsGST-fusion proteinExpressionBacterialMutationchanged lysine 119 to alanineAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEf1a OsTIR ires NEO pA T2BH
Plasmid#127910PurposeTransposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoterDepositorInsertOsTIR
UseTransposonAvailable SinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-Hyg-Dest-mCherry-PAR-6B
Plasmid#194908PurposeTo generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.DepositorInsertPAR-6B (Pard6b Mouse)
UseMouse TargetingTagsmCherryExpressionMammalianMutation39 C to G, silent mutationAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hTGM3-Flag
Plasmid#180408Purposelentiviral transduction of human TGM3 (with a C-terminal Flag tag) geneDepositorAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330 Human 3' HP1a gRNA
Plasmid#127907PurposeWT Cas9 Vector targeting the 3' end of the human HP1a geneDepositorInsertgRNA for Human 3' HP1a
UseCRISPRAvailable SinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-NLS-Cre-2A-mCherry-FKF
Plasmid#119868PurposeDonor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertNLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
TagsmCherryExpressionBacterialAvailable SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET19-MTdTwt
Plasmid#207460PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT)DepositorAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDT386
Plasmid#174733PurposeExpresses His-tagged DT386, a truncated diphtheria toxin without a receptor-binding domain.DepositorInsertDT386
TagsHistagExpressionBacterialMutationDeleted amino acids 412-560 (the receptor binding…PromoterT5Available SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_WT RAD23B
Plasmid#201439PurposeExpresses human RAD23B with an N-terminal FLAG tag in mammalian cellsDepositorInsertUV excision repair protein RAD23 homolog B (RAD23B Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-mycRSK2wt
Plasmid#15827DepositorInsertRibosomal S6 kinase 2 (Rps6ka3 Mouse)
UseRetroviralTagsmycExpressionMammalianMutationwild typeAvailable SinceNov. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
CMV-Tagger
Plasmid#129396PurposeExpresses 2A-separated rpl22, UPRT, mKate2 and Ago2 driven by CMV promoterDepositorInsertrpl22-HA-2A-UPRT-mKate2-FLAG-V5-Ago2
TagsFLAG, HA, and V5ExpressionMammalianPromoterCMVAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCWXPG-UBI-MafA
Plasmid#114302PurposeConstitutive expression of the MafA trancription factorDepositorInsertMafA (MAFA Human)
UseLentiviralAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-shRNA_Tet3
Plasmid#85740PurposeshRNA against mouse Tet3DepositorAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only