163,606 results

1. pT3TS nErCas12an

   Plasmid

   #132642

   Purpose

   Used for in vitro transcription of dual NLS ErCas12a mRNA

   Depositor

   Stephen Ekker

   Article

   Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

   Insert

   ErCas12a
   

   Use

   CRISPR; In vitro transcription

   Tags

   SV40 NLS

   Expression

   Mutation

   Promoter

   T3

   Available Since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSFFV_CloGFP(1-10)

   Plasmid

   #157994

   Purpose

   Lentiviral vector of constitutive expression of CloGFP(1-10)

   Depositor

   Bo Huang

   Article

   Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

   Insert

   CloGFP(1-10)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   G32Q, F46L, K101R, S202L

   Promoter

   SFFV

   Available Since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFRT/TO/HIS/FLAG/HA-MYBBP1A

   Plasmid

   #38084

   Depositor

   Markus Landthaler

   Article

   Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

   Insert

   MYBBP1A MYB binding protein (P160) 1a (MYBBP1A Human)
   

   Use

   Tags

   HIS/FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-puro

   Plasmid

   #45561

   Depositor

   Michael McVoy

   Article

   Abbate et al Biotechniques. 2001 Aug;31(2):336-40.

   Insert

   Puromycin N-acetyl transferase
   

   Use

   Bifunctional selection protein

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Phcmv (Strong human cytomegalovirus immediate ear…

   Available Since

   June 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX317-VRK1

   Plasmid

   #199643

   Purpose

   Expresses VRK1

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   VRK1 (VRK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLpA-DN.JNK1

   Plasmid

   #51942

   Purpose

   Adenoviral vector for dominant negative JNK1 expression

   Depositor

   Jeffery Molkentin

   Article

   Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.

   Insert

   DN JNK1 (Mapk8 Mouse)
   

   Use

   Adenoviral

   Tags

   FLAG

   Expression

   Mutation

   dominant negative: the dual phosphorylation moti…

   Promoter

   CMV

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

7. miRFP670nano-pcDNA

   Plasmid

   #127443

   Purpose

   Monomeric near-infrared fluorescent protein miRFP670nano in mammalian plasmid

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   miRFP670nano
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-CMV-NES-jRGECO1a

   Plasmid

   #61563

   Purpose

   Red fluorescent calcium sensor protein

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Insert

   NES-jRGECO1a
   

   Use

   Tags

   nuclear export signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-ADAM17-HA

   Plasmid

   #65105

   Purpose

   Expression of HA tagged ADAM17 (TACE) in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

   Insert

   ADAM17 (ADAM17 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG_iPE-N

    Plasmid

    #214734

    Purpose

    encodes iPE-N prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the N terminus of nCas9-H840A) driven by a CAG promoter

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

    Insert

    CAG_iPE-N_bGH
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-CMV-Galpha-i2 ONE-GO

    Plasmid

    #189736

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-i2 ONE-GO
    GNAI2 (Gnai2 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pHR-scFv-GCN4-sfGFP-GB1-dWPRE

    Plasmid

    #60907

    Purpose

    The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP for imaging

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    scFv-GCN4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. Hs.KRAS4b G12D

    Plasmid

    #83131

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12D mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    G12D

    Promoter

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. PiggyBac-TetO-Lmx1a-t2a-Pitx3-puro

    Plasmid

    #176484

    Purpose

    TetO-Lmx1a-t2a-Pitx3 in a PiggyBac vector with puromycin resistance

    Depositor

    Marius Wernig

    Article

    Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

    Insert

    Lmx1A-T2A-Pitx3 (PITX3, LMX1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDule2-Acd82

    Plasmid

    #174100

    Purpose

    Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

    Depositor

    Ryan Mehl

    Article

    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

    Insert

    Acridone 82 tRNA synthetase and tRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N311S, C313G, V366A, W382T

    Promoter

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHREAC

    Plasmid

    #134908

    Purpose

    Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Download fully annotated map under Resource Information.

    Depositor

    George Lomonossoff

    Article

    Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    double 35S

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. PA-mCherry1-H2B-C-10

    Plasmid

    #57181

    Purpose

    Localization: Histones/Nucleus, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    PA-mCherry1

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTPH418b

    Plasmid

    #185725

    Purpose

    Directed evolution of Cas9 adenine base editors with altered PAM compatibility

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    gIII(N)-NpuN-NpuC-gIII(C)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Hs.KRAS4b

    Plasmid

    #83129

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG_iPE-C

    Plasmid

    #214735

    Purpose

    encodes iPE-C prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the C terminus of nCas9-N863A) driven by a CAG promoter

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

    Insert

    CAG_iPE-C_bGH
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pT3TS-spCas9-ABE-Umax-rest1

    Plasmid

    #222144

    Purpose

    Adenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pT7-EGFP-C1-HsXRN1_K

    Plasmid

    #146784

    Purpose

    Mammalian Expression of HsXRN1

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

    Insert

    HsXRN1 (XRN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAWP78

    Plasmid

    #61263

    Purpose

    Small IncP-based broad host range replicating vector.

    Depositor

    Mary Lidstrom

    Article

    Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTPH412

    Plasmid

    #185724

    Purpose

    Directed evolution of Cas9 adenine base editors with altered PAM compatibility

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-gp41-8N
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    ecTadA(8e) R26G

    Promoter

    Available Since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGL0_12 [pAMβ1 ORI]

    Plasmid

    #198926

    Purpose

    Level 0 part

    Depositor

    Charlie Gilbert, Cultivarium Tools, Nili Ostrov

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pAMβ1 ORI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. elavl3:hArr-TEV;he1.1:YFP

    Plasmid

    #218855

    Purpose

    D. rerio elavl3 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.

    Depositor

    Marnie Halpern

    Article

    Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

    Insert

    hArr
    

    Use

    Tags

    human Arrestin-Tobbaco Etch Virus protease fusion…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLVTHM

    Plasmid

    #12247

    Purpose

    2nd gen lentivector expressing shRNA from H1 promoter and GFP. For direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from pSUPER. Dox-controllable if used with tTRKRAB expressing plasmid

    Depositor

    Didier Trono

    Article

    Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-Ef1a-ConVERGD-eGFP-WPRE

    Plasmid

    #218745

    Purpose

    Provides AND intersectional (Cre+Flp) expression of eGFP

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody

    Plasmid

    #138130

    Purpose

    Expresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    mCherry-anti-mouse-IgG(kappa)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. IDG_CACNA2D2_OE_1

    Plasmid

    #161616

    Purpose

    Overexpresses CACNA2D2

    Depositor

    Mike McManus

    Insert

    CACNA2D2 (CACNA2D2 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHR-UCOE-EF1a-Zim3-dCas9-loxP-P2A-EGFP-loxP

    Plasmid

    #188773

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. SlugCas9

    Plasmid

    #163793

    Purpose

    Expresses SlugCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pT2KXIG (Tol2)-tp63:ikk1:GFP

    Plasmid

    #105646

    Purpose

    Ikk1(alpha) expression in basal keratinocytes

    Depositor

    Sandra Rieger

    Article

    Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

    Insert

    tp63:ikk1:GFP (chuk Zebrafish)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1(+)_Pmel-1_TCRB

    Plasmid

    #175288

    Purpose

    Expresses Pmel-1 TCR beta chain

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    T cell receptor beta chain (Tcrb Mouse)
    

    Use

    Suitable for ivt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1(+)_Pmel-1_TCRA

    Plasmid

    #175287

    Purpose

    Expresses Pmel-1 TCRA alpha chain

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    T cell receptor alpha chain (Tcra Mouse)
    

    Use

    Suitable for ivt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAlbumin

    Plasmid

    #22037

    Depositor

    Didier Trono

    Insert

    partial sequence of human albumine
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. plRL61

    Plasmid

    #163633

    Purpose

    Optimized M. smegmatis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634).

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet-On

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. TFORF0952

    Plasmid

    #143894

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pWZL Neo Myr Flag PLK1

    Plasmid

    #20589

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    PLK1 (PLK1 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-PuroR-SMAR-g5

    Plasmid

    #208377

    Purpose

    Episomal plasmid for expression of 2 proteins with puromycin selection and EGFP monitoring

    Depositor

    Daniel Hawiger

    Article

    Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human CMV

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge