Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 31,648 results for: REP

Clear keywords
Showing: 5551 - 5600 of 31648 results

  7. pFUS-WT-VC

    Plasmid
    #175068
    Purpose
    expresses FUS wildtype tagged to mVenus and mCherry in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    FUS glycine rich protein (FUS Human)
    Tags
    HA and mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Available Since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175068-plasmid-map-sequence-id-353791

    Enlarge

  9. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A

    Plasmid
    #51257
    Purpose
    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248,789,805,817,848,923,956 mutated to Ala
    Depositor
    Rajat Rohatgi
    Article
    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1
    Insert
    Gli2 (Gli2 Mouse)
    Use
    Flpin system
    Tags
    3xHA-TEV
    Expression
    Mammalian
    Mutation
    amino acids 216,220,224,230,248,789,805,817,848,9…
    Promoter
    EF1a
    Available Since
    Feb. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51257-plasmid-map-sequence-id-501235

    Enlarge

  12. pCC_10 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139095
    Purpose
    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dSpCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139095-plasmid-map-sequence-id-273892

    Enlarge

  20. pVITRO1-102.1F10-IgG4/λ

    Plasmid
    #50369
    Purpose
    Expresses grass pollen allergen Phl p 7 specific human IgG4/λ antibody isotype (102.1F10-IgG4/λ)
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    grass pollen allergen Phl p 7 specific human Gamma 4 heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    Expression
    Mammalian
    Promoter
    Mouse Elongation Factor 1 Alpha and Rat Elongatio…
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50369-plasmid-map-sequence-id-368213

    Enlarge

  21. pCSIIpuro-EREG-ScNeo

    Plasmid
    #209896
    Purpose
    To monitor the status of Epiregulin, the plasmid encodes a recombinant Epiregulin fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    EREG-ScNeo (EREG Human)
    Use
    Lentiviral
    Tags
    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…
    Available Since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209896-plasmid-map-sequence-id-416598

    Enlarge

  22. pAAV hPGRN

    Plasmid
    #213682
    Purpose
    Expresses human progranulin (PGRN) with an N-terminal twin-Strep-V5 tag
    Depositor
    Thomas Kukar
    Article
    Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi
    Insert
    Human Progranulin (hPGRN) (GRN Human)
    Use
    AAV
    Tags
    Twin-Strep tag and V5 tag (after signal peptide)
    Promoter
    cytomegalovirus enhancer/chicken β-actin promoter
    Available Since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    213682-plasmid-map-sequence-id-455751

    Enlarge

  28. AAV-Dlx-SynaptoTAG

    Plasmid
    #210729
    Purpose
    AAV vector for mapping synaptic projections. GABAergic neuron-specific Dlx promoter expresses EGFP-Synaptobrevin-2 to label synaptic terminals and Palm-tdTomato to label neuronal soma and axons.
    Depositor
    Wei Xu
    Article
    Du et al Cell Rep Methods. 2023 Jun 16;3(6):10050
    Insert
    EGFP-Synaptobrevin-2, P2A, Palm-tdTomato (tdTomato with Palmitoylation sequence) (Vamp2 Synthetic, Rat)
    Use
    AAV
    Promoter
    Dlx
    Available Since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    210729-plasmid-map-sequence-id-418041

    Enlarge

  31. pCSIIpuro-EGF-ScNeo

    Plasmid
    #209893
    Purpose
    To monitor the status of EGF, the plasmid encodes a recombinant EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    EGF-ScNeo (EGF Human)
    Use
    Lentiviral
    Tags
    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…
    Available Since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209893-plasmid-map-sequence-id-416580

    Enlarge

  35. pPBbleo-EREG-ScNeo

    Plasmid
    #209905
    Purpose
    To monitor the status of Epiregulin, the plasmid encodes a recombinant Epiregulin fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    EREG-ScNeo (EREG Human)
    Tags
    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…
    Expression
    Mammalian
    Available Since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209905-plasmid-map-sequence-id-416688

    Enlarge

  37. pelB-MBP-HtrA-ss

    Plasmid
    #137066
    Purpose
    E. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature HtrA: lacks the HtrA signal peptide
    Promoter
    T7lac
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137066-plasmid-map-sequence-id-291956

    Enlarge

  38. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139089
    Purpose
    Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    xCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139089-plasmid-map-sequence-id-273885

    Enlarge

Showing: 5551 - 5600 of 31648 results