175,581 results

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pET-His6-ERK2-MEK1_R4F_coexpression

Plasmid

#39212

Depositor

Melanie Cobb

Article

Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

Inserts

Mek1 R4F (MAP2K1 Human)
Erk2 (Mapk1 Rat)

Tags

His6 and RBS

Expression

Bacterial

Mutation

MEK1(R4F) = S218E, S222D and deletion of AA32-51

Promoter

T7 and T7 via RBS

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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FLAG-SNAP29

Plasmid

#45915

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

synaptosomal-associated protein, 29kDa (SNAP29 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pHes1(2.5k)-luc

Plasmid

#43806

Depositor

Ryoichiro Kageyama

Article

Takebayashi et al J Biol Chem. 1994 Feb 18;269(7):5150-6.

Insert

Hes1 Promoter (Hes1 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Contains the murine Hes1 promoter

Promoter

Hes1 Promoter

Available Since

March 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-TVA-mCherry

Plasmid

#38044

Depositor

Naoshige Uchida

Article

Watabe-Uchida et al Neuron. 2012 Jun 7;74(5):858-73.

Insert

Avian sarcoma and leukemia virus receptor 950 (CD320 Synthetic, quail)

Use

AAV and Cre/Lox

Tags

mCherry

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti 4.1 Ex miR200c-141

Plasmid

#35534

Purpose

3rd generation lentiviral vector

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

miR200c-141 genomic cluster (MIR200C, MIR141 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2012

Availability

Academic Institutions and Nonprofits only

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p3HA-hMIB1

Plasmid

#33317

Depositor

Vanessa Redecke

Article

Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

Insert

MIB1 (MIB1 Human)

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pEB-p53shRNA

Plasmid

#28222

Depositor

Linzhao Cheng

Article

Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

Insert

p53 shRNA

Expression

Mammalian

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only
pCI HA NEDD4 DD

Plasmid

#26999

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4 (NEDD4 Human)

Tags

HA

Expression

Mammalian

Mutation

C867A

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Sox2 3H b

Plasmid

#26352

Depositor

Matthew Meyerson

Article

Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

Insert

Sox2 shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pMX-Brg1

Plasmid

#25855

Depositor

Hans Schöler

Article

Singhal et al Cell. 2010 Jun 11. 141(6):943-55.

Insert

Brahma related gene 1 (Smarca4 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pLL3.7_hsa-miR-34a

Plasmid

#25791

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

hsa-miR-34a (TP53 Human)

Use

Lentiviral and RNAi

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-fPV-GFP

Plasmid

#22914

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu parvalbumin promoter driving GFP

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pCF CREB M1

Plasmid

#22969

Depositor

Marc Montminy

Article

Du et al Mol Cell Biol. 2000 Jun . 20(12):4320-7.

Insert

CREB (Creb1 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

Changed Serine 133 to Alanine

Available Since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

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HA-Elongin B-pcDNA3.1(+)-Zeo

Plasmid

#20000

Depositor

William Kaelin

Article

Ohh et al Nat Cell Biol. 2000 Jul . 2(7):423-7.

Insert

transcription elongation factor B (ELOB Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wild type

Available Since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA3-TSC1

Plasmid

#19911

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

Tsc1 (Tuberous sclerosis 1) (TSC1 Human)

Tags

HA

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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Lyn-FKBP-FKBP-CFP (LF2C)

Plasmid

#20149

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

Lyn-FKBP-FKBP-CFP (FKBP1A Human)

Expression

Mammalian

Mutation

See depositor comments below.

Available Since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabePuro-hSLUG.ER.NoTag

Plasmid

#19293

Depositor

Eric Fearon

Article

Hajra et al Cancer Res. 2002 Mar 15. 62(6):1613-8.

Insert

SLUG (SNAI2 Human)

Use

Retroviral

Tags

mouse ERalpha ligand-binding domain

Expression

Mammalian

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
YPET-GGA3

Plasmid

#18841

Depositor

Martin Schwartz

Article

Hall et al Anal Biochem. 2008 Mar 15. 374(2):243-9.

Insert

YPET-GGA3 (GGA3 Human)

Tags

His6 and myc

Expression

Mammalian

Mutation

The fluorescent protein YPET was added to the ami…

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-Neo-Bam p53 V143A

Plasmid

#16435

Depositor

Bert Vogelstein

Article

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Insert

p53 V143A (TP53 Human)

Expression

Mammalian

Mutation

V143A. Mutant cDNA from human colorectal tumor CX…

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pGL2-356bp

Plasmid

#16292

Depositor

Wafik El-Deiry

Article

Wang et al Cancer Res. 2006 Jul 15. 66(14):6982-9.

Insert

p53 promoter fragment (TP53 Human)

Use

Luciferase

Expression

Mammalian

Mutation

fragment -344 to +12 relative to first transcript…

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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GFP Snail 6SA

Plasmid

#16228

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

6SA: S97A, S101A, S108A, S112A, S116A, S120A

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pRK5 HA GST Rheb1

Plasmid

#14951

Depositor

David Sabatini

Article

Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15.

Insert

Rheb1 (RHEB Human)

Tags

HA-GST

Expression

Mammalian

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PBD-EYFP

Plasmid

#13723

Depositor

Klaus Hahn

Article

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

PBD-EYFP (PAK1 Human)

Tags

EYFP

Expression

Mammalian

Mutation

p21 binding domain of human Pak1(amino acids 65-1…

Available Since

March 28, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
LL - hOCT4i -1

Plasmid

#12198

Depositor

George Daley

Article

Zaehres et al Stem Cells. 2005 Mar . 23(3):299-305.

Insert

OCT4 - RNAi (POU5F1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Sept. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro CXCR4 siRNA-1

Plasmid

#12271

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

CXCR4 siRNA (CXCR4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Casp2-Flag

Plasmid

#11811

Depositor

Guy Salvesen

Insert

Caspase-2 (CASP2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5B-FLAG-SARA

Plasmid

#11738

Depositor

Jeff Wrana

Article

Tsukazaki et al Cell. 1998 Dec 11. 95(6):779-91.

Insert

SARA (ZFYVE9 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
1051 pBabe puroL Akt

Plasmid

#9011

Depositor

William Sellers

Article

DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

Insert

Akt1 (AKT1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
1272 pBabe puroL Myr HA Akt3

Plasmid

#9019

Depositor

William Sellers

Insert

Akt3 (AKT3 Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
1072 pBabe puroL myr Akt K179M T308A S473A

Plasmid

#9015

Depositor

William Sellers

Article

DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

Insert

Akt1 K179M T308A S473A (AKT1 Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Mutation

K179M T308A S473A

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
MLV Gag-RFP

Plasmid

#1814

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

Gag (gag MuLV)

Tags

RFP

Expression

Mammalian

Available Since

May 19, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3/mStayGold(c4)=UtrCH

Plasmid

#212020

Purpose

For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

UtrCH

Tags

mStayGold(c4)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Fos-CreERT2

Plasmid

#194643

Purpose

Expresses Cre-ERT2 under the control of c-Fos promoter

Depositor

Jun-Hyeong Cho

Article

Lee et al Nat Neurosci. 2022 Dec 23. doi: 10.1038/

Insert

Fos-CreERT2

Use

AAV

Expression

Mammalian

Available Since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-PS9

Plasmid

#177944

Purpose

Transient mammalian expression of eGFP along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

eGFP (ORF1ab )

Expression

Mammalian

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Fab_8D3_2_H-H6

Plasmid

#176075

Purpose

Express His6 tagged heavy chain of Fab_8D3_2 in mammalian cells

Depositor

Tom Rapoport

Article

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

His6 tagged heavy chain of Fab_8D3_2

Tags

His tag

Expression

Mammalian

Promoter

CAG promoter

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Fab_8D3_2_L

Plasmid

#176076

Purpose

Express light chain of Fab_8D3_2 in mammalian cells

Depositor

Tom Rapoport

Article

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

Light chain of Fab_8D3_2

Expression

Mammalian

Promoter

CAG promoter

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Frankenbody-FLAG-mRuby2

Plasmid

#175294

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with mRuby2

Tags

mRuby2

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCW-Cas9-2A-EGFP

Plasmid

#167928

Purpose

Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Insert

T2A-EGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

tTRE, hPGK

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZac2.1-GfaABC1D-TurboID(full)-HA-GPI

Plasmid

#166055

Purpose

Expresses the full length TurboID on the surface of astrocytes

Depositor

Scott Soderling

Article

Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

Insert

GPI anchored TurboID

Use

AAV

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-puro/Flag PD-L1 WT

Plasmid

#121446

Purpose

expression of huamn PD-L1 wildtype in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

wildtype

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV.CBA.eGFP.2A.wtTau

Plasmid

#140424

Purpose

AAV.CBA.eGFP.2A.wtTau propagation reporter WT-2N4R-hTau

Depositor

Bradley Hyman

Article

Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

Insert

eGFP-2A-Tau (MAPT Synthetic, Human)

Use

AAV

Tags

eGFP

Promoter

CBA

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLARRY-EGFP

Plasmid

#140025

Purpose

Lentiviral backbone for cloning expressed barcodes in the 3'UTR of the EGFP mRNA

Depositor

Fernando Camargo

Article

Weinreb et al Science. 2020 Jan 23. pii: science.aaw33

Insert

EGFP

Use

Lentiviral

Promoter

EF1alpha

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pUCmini-iCAP-PHP.V1

Plasmid

#127847

Purpose

non-standard AAV2 rep-AAV-PHP.V1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

Insert

Synthetic construct isolate AAV-PHP.V1 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SaKKH-ABE8e

Plasmid

#138502

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSaCas9 (KKH)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR-cBEST

Plasmid

#125689

Purpose

C to T base editor for actinomycetes

Depositor

Tilmann Weber

Article

Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

Insert

streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-EGFP-2xFYVE

Plasmid

#136996

Purpose

Lentiviral EGFP lipid sensor for PI(3)P

Depositor

Ken-Ichi Takemaru

Insert

EGFP-2xFYVE

Use

Lentiviral

Tags

EGFP

Promoter

EF1 alpha

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SpyTag003-MBP

Plasmid

#133450

Purpose

Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-MBP

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

Peptide tag added to N-terminus of MBP via a GSGE…

Promoter

T7

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRN114

Plasmid

#127221

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, Wus, AtSTM

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-6XHis-human SUMO 2

Plasmid

#133771

Purpose

express 6Xhis- SUMO 2 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 2 (SUMO2 Human)

Tags

6XHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX401-INK4A

Plasmid

#121919

Purpose

Doxycycline-inducible expression of p16-INK4A product of CDKN2A

Depositor

William Hahn

Article

Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

Insert

p16-INK4A (CDKN2A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE Tet ON

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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175,581 results