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177,160 results

Showing: 5601 - 5650 of 177160 results

  1. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid
    #107943
    Purpose
    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    Tags
    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    107943-plasmid-map-sequence-id-207614

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  6. LZ10 PBREBAC-H2BHalo

    Plasmid
    #91564
    Purpose
    piggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.
    Depositor
    Zhe J. Liu
    Publication
    Li et al Elife. 2016 Aug 3;5. pii: e17056. doi: 1
    Insert
    histone H2B-HaloTag
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    EF1
    Available since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    91564-plasmid-map-sequence-id-180643

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  9. pcDNA3.1-ccdB-Nanoluc

    Plasmid
    #87067
    Purpose
    Mammalian Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase
    Depositor
    Mikko Taipale
    Type
    Empty backbone
    Tags
    Nanoluc luciferase
    Expression
    Mammalian
    Available since
    March 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    13 Articles
    87067-plasmid-map-sequence-id-192710

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  12. pML104-KanMx4

    Plasmid
    #83476
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    KanMX4
    Expression
    Yeast
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    83476-plasmid-map-sequence-id-189195

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  13. pH2B-miRFP703

    Plasmid
    #80001
    Purpose
    Histone 2B labeling with near-infrared fluorescent protein miRFP703
    Depositor
    Vladislav Verkhusha
    Publication
    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    histone cluster 1 H2B family member j (H2BC11 Human)
    Tags
    miRFP703
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    80001-plasmid-map-sequence-id-157294

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  15. pHLmMBP-10

    Plasmid
    #72348
    Purpose
    Mammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Publication
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Tags
    6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…
    Expression
    Mammalian
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    72348-plasmid-map-sequence-id-133757

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  16. mKate2-P2A-APEX2-GBP

    Plasmid
    #67650
    Purpose
    Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.
    Depositor
    Rob Parton
    Publication
    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    APEX2-GBP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    67650-plasmid-map-sequence-id-116990

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  18. OPRM1-Tango

    Plasmid
    #66464
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OPRM1 (OPRM1 Human)
    Tags
    FLAG and tTA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    66464-plasmid-map-sequence-id-157903

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  23. Villinpromoter-blue-FlpOERT2

    Plasmid
    #67278
    Purpose
    4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context
    Depositor
    Jannik Elverløv-Jakobsen
    Publication
    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do
    Insert
    Villin-mTQ2-P2A-FlpO-ERT2
    Tags
    mTQ2, linked to FlpO through an P2A ribosomal ski…
    Expression
    Mammalian
    Promoter
    Villin
    Available since
    July 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    67278-plasmid-map-sequence-id-115887

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  25. GFP-BAZ1B

    Plasmid
    #65372
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BAZ1B (BAZ1B Human)
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    June 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    65372-plasmid-map-sequence-id-366630

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  26. GFP-BRD7

    Plasmid
    #65379
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BRD7 (BRD7 Human)
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    65379-plasmid-map-sequence-id-422714

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  28. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61356-plasmid-map-sequence-id-498727

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  32. mCherry-Calnexin-N-14

    Plasmid
    #55005
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Calnexin (CANX Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    I474N in Calnexin
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    55005-plasmid-map-sequence-id-456544

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  34. mEmerald-Alpha-V-Integrin-25

    Plasmid
    #53985
    Purpose
    Localization: Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Alpha-V Integrin (ITGAV Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles
    53985-plasmid-map-sequence-id-257384

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  36. pBABEpuro-CRAF

    Plasmid
    #51124
    Purpose
    mammalian expression of wt CRAF
    Depositor
    Matthew Meyerson
    Insert
    CRAF (RAF1 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    51124-plasmid-map-sequence-id-261155

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  40. pETcon(-)

    Plasmid
    #41522
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Tags
    3x(G4S) Linker, G3S Linker, HA, Myc Tag, and Stuf…
    Expression
    Yeast
    Promoter
    GAL1
    Available since
    April 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    22 Articles
    41522-plasmid-map-sequence-id-59598

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Showing: 5601 - 5650 of 177160 results