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pBetaActin-TUBB2a-mScarlet

Plasmid

#191324

Purpose

Expresses fluorescent labeled beta Tubulin to measure microtubule density

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mScarlet (Tubb2a Mouse)

Expression

Mammalian

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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mARG1 cassette 3

Plasmid

#134345

Purpose

Mammalian acoustic reporter gene 1 cassette 3 on the piggyBac trasposon plasmid.

Depositor

Mikhail Shapiro

Article

Farhadi et al Science. 2019 Sep 27;365(6460):1469-1475

Insert

mARG 1 cassete 3

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP12042

Plasmid

#222354

Purpose

Tn7 integrative vector with PabstBR promoter expression cassette and aprR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Type

Empty backbone

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-AP(-3)-Ub

Plasmid

#232583

Purpose

Express Ubiquitin tagged with a modified Acceptor Peptide (AP)

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

Ubiquitin

Tags

AP

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF-KpAGO207-1251

Plasmid

#117854

Purpose

Expressed KpAGO 207-1251 in E. coli under IPTG-inducible promotor

Depositor

Kotaro Nakanishi, Dinshaw Patel

Article

Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

Insert

Argonaute

Tags

6xHis-SUMO encoded in vector

Expression

Bacterial

Mutation

missing first 206 amino acid residues

Promoter

T7

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pKM493

Plasmid

#140191

Purpose

ORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

TEV-Flag-Gly4-eGFP

Tags

Flag + eGFP

Expression

Bacterial

Promoter

PGroEL

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pSEVA23-GG

Plasmid

#208013

Purpose

for cloning homology arms, with GFP as counter selection marker

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Insert

msfGFP

Promoter

14G

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->myc-mtLaG2

Plasmid

#162224

Purpose

Lentiviral vector for constitutive expression of Myc GFP nanobody Lag2 in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

anti-GFP nanobody LaG2

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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EpAvs1

Plasmid

#188788

Purpose

Avs1 from Erwinia piriflorinigrans CFBP5888

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EpAvs1

Expression

Bacterial

Promoter

bla

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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C1-CFP-Lyn11

Plasmid

#155234

Purpose

membane marker

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lyn11 membrane targeted CFP. Membrane control

Tags

CFP

Expression

Mammalian

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pAX1

Plasmid

#117397

Purpose

allelic exchange vector with GFP merodiploid tracker and temperature-sensitive origin of replication

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pBSV2B

Plasmid

#118228

Purpose

Empty E. coli - B. burgdorferi shuttle vector, blasticidin S resistant

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pHR_Ef1a-->flagLaM4_Notch_tTA

Plasmid

#162237

Purpose

Lentiviral vector for the expression of LaM 4 mCherry nanobody notch tTA: flagLaM4_Notch_tTA in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

LaM4 mCherry nanobody Notch tTA

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pHIP_RNA2FLAG

Plasmid

#61261

Purpose

Flag tagged Orsay virus RNA2 genome segment expression plasmid

Depositor

David Wang

Article

Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

Insert

Orsay RNA2

Tags

Flag fused

Expression

Worm

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pHR_TRE3GS-->sGFP-P2A-mCherry

Plasmid

#162232

Purpose

Lentiviral vector for the expression of TRE3GS-->sGFP-P2A-mCherry in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

EGFP and mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_TRE3GS-->sGFP-P2A-BFP

Plasmid

#162234

Purpose

Lentiviral vector for the expression of TRE3GS-->sGFP-P2A-BFP in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

EGFP and BFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pKEW189-MBP-TEV-AcrVA1

Plasmid

#115660

Purpose

Expresses AcrVA1 in bacteria via T7 RNAP, for purification

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA1

Tags

MBP-TEV

Expression

Bacterial

Promoter

T7 RNAP

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pET-mKelly1

Plasmid

#119782

Purpose

Expresses mKelly1 tagged N-terminally with an MHHHHHHGSG tag

Depositor

Stephen Mayo

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

Insert

mKelly1

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pET-mGinger1

Plasmid

#119784

Purpose

Expresses mGinger1 tagged N-terminally with an MHHHHHHGSG tag

Depositor

Stephen Mayo

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

Insert

mGinger1

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAC-BETAipi-ispA/dxs

Plasmid

#178302

Purpose

Produces beta-carotene in E. coli; pAC-BETAipi inserted with an operon containing ispA and dxs from E. coli.

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Inserts

ispA
dxs

Expression

Bacterial

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-SwiChRca-TS-EYFP

Plasmid

#55630

Purpose

Chloride Channel

Depositor

Karl Deisseroth

Article

Berndt et al Science. 2014 Apr 25;344(6182):420-4. do

Insert

iC1C2-TS-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

C128A

Promoter

CaMKIIa

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pJMP3666

Plasmid

#222352

Purpose

Expression vector with GFP expressed under PabstBR promoter, aprR marker, and replicates in A. baumannii and E. coli.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Insert

sfGFP

Expression

Bacterial

Promoter

PabstBR

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-RanV19

Plasmid

#118316

Purpose

Expression of human Ran

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

RanV19 (RAN Human)

Tags

mCherry

Expression

Mammalian

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-hG9a-ΔSET

Plasmid

#33026

Depositor

Martin Walsh

Article

Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

Insert

hG9a

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the SET domain; contains aa 1-758

Promoter

CMV

Available Since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pSEVA13-GG

Plasmid

#208002

Purpose

for cloning homology arms, with GFP as counter selection marker

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Insert

msfGFP

Promoter

14G

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSt-KO

Plasmid

#44566

Purpose

for generation of knockout of mycobacterial genes

Depositor

Vinay Nandicoori

Article

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Expression

Bacterial

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pMO719

Plasmid

#117479

Purpose

Desulfovibrio shuttle vector, contains pBG1 and pMB1 replicons, specR

Depositor

Judy Wall

Article

Keller et al Appl Environ Microbiol. 2009 Dec;75(24):

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRL-ubi63E

Plasmid

#74280

Purpose

Renilla control plasmid driven by the ubiquitin 63E promoter

Depositor

Alexander Stark

Article

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Insert

Renilla luciferase

Expression

Insect

Promoter

ubiquitin 63E promoter

Available Since

April 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP6f-P2A-PAmCherry

Plasmid

#133412

Purpose

Fluorescent reporter for calcium imaging and photoactivation.

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-PAmCherry

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTrig-CopA

Plasmid

#104578

Purpose

Copy number inducible plasmid. Contains mini-F origin for maintenance and P1 lytic replication machinery under CopA promoter for copy increase.

Depositor

Harris Wang

Article

Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

Insert

PCopA-RiboJ-B0034-repL

Use

Synthetic Biology

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmaxAW

Plasmid

#125647

Purpose

Expresses ABEmaxAW in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A, TadA* V106W)

Expression

Mammalian

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6C

Plasmid

#42023

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6C (TNRC6C Human)

Tags

EGFP and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
lenti-CHMP4B gRNA2

Plasmid

#211629

Purpose

sgRNA-2 against CHMP4B

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA CHMP4B

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-CHMP4B gRNA1

Plasmid

#211628

Purpose

sgRNA-1 against CHMP4B

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA CHMP4B

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p8267 LentiCRISPR v2 hygro sgNT-2

Plasmid

#193978

Purpose

Expression of spCas9 and non-targeting control sgRNA

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

spCas9

Use

Lentiviral

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pstb-LAFR5

Plasmid

#86169

Purpose

pLAFR5 with Smb21651 promoter. pLAFR5 is a broad-host range cosmid vector with double cos sites.

Depositor

Kay Jones

Article

Jones et al J Bacteriol. 2012 Aug;194(16):4322-31. d

Type

Empty backbone

Expression

Bacterial

Promoter

SMb21651 promoter

Available Since

Feb. 6, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3.1 3xF-NHP2

Plasmid

#126871

Purpose

Expresses 3xFlag NHP2

Depositor

Kathleen Collins

Article

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

NHP2 (NHP2 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAH01Sp::Cas9D10A

Plasmid

#128161

Purpose

Ptet-controlled expression of S. pyogenes Cas9D10A variant

Depositor

Calvin Henard

Article

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Insert

Sp Cas9

Expression

Bacterial

Mutation

D10A

Promoter

Ptet

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP8045

Plasmid

#220900

Purpose

Tn7 transposase (tnsABCD) expression plasmid for Rhodobacter sphaeroides

Depositor

Jason Peters

Article

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

tnsABCD

Expression

Bacterial

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2H

Plasmid

#118229

Purpose

Empty E. coli - B. burgdorferi shuttle vector; hygromycin B resistant

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEVY-GT

Plasmid

#51231

Purpose

bi-directional expression vector for yeast, galactose inducible, TRP1 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GAL1,10

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pEQ1180

Plasmid

#83805

Purpose

express human cytomegalovirus TRS1 with a C-terminal 6X his tag in eukaryotic cells

Depositor

Adam Geballe

Article

Child et al J Virol. 2012 Apr;86(7):3880-9. doi: 10.

Insert

TRS1

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
pGL3-MMLV-LTR-Luc

Plasmid

#67831

Purpose

Expression of luciferase under the control of MMLV viral LTRs

Depositor

Stephen Goff

Article

Wang et al J Virol. 2015 Mar;89(5):2813-9. doi: 10.

Insert

MMLV-LTR + primer binding site

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
pNHA5

Plasmid

#223647

Purpose

TurboID protein purification

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Insert

TurboID

Tags

6His

Expression

Bacterial

Promoter

LacI

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pNHA6

Plasmid

#223648

Purpose

BioID protein purification

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Insert

BioID

Tags

6His

Expression

Bacterial

Promoter

LacI

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCDNA3_Gap43_Csx30_Cre

Plasmid

#197604

Purpose

Cre reporter for mammalian cells with Gap43 membrane anchor

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

Gap43_Csx30_Cre

Expression

Mammalian

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKEW212-MBP-TEV-AcrVA4

Plasmid

#115658

Purpose

Expresses AcrVA4 in bacteria via T7 RNAP, for purification

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA4

Tags

MBP-TEV

Expression

Bacterial

Promoter

T7 RNAP

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV2-GCaMP5g-P2A-PAmCherry

Plasmid

#133411

Purpose

Fluorescent reporter for calcium imaging and photoactivation.

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP5g-P2A-PAmCherry

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP12049

Plasmid

#222355

Purpose

Tn7 integrative vector with GFP expressed under PabstBR promoter and aprR marker on Tn7 transposon.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Insert

sfGFP

Expression

Bacterial

Promoter

PabstBR

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PNCA-luciferase

Plasmid

#67793

Purpose

Use together with pCMV-intron and VSV-G to package MMLV reporter virus

Depositor

Stephen Goff

Article

Wang et al J Virol. 2015 Mar;89(5):2813-9. doi: 10.

Insert

MMLV-LTR-firefly luciferase

Expression

Mammalian

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

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We narrowed to 12,598 results for: 112