4,133 results

1. pBabe puro Myr HA PIK3CA

   Plasmid

   #12523

   Depositor

   Jean Zhao

   Article

   Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

   Insert

   PIK3CA Myr (PIK3CA Human)
   

   Use

   Retroviral

   Tags

   HA and Myr

   Expression

   Mammalian

   Mutation

   Myristoylation. Constitutively active allele; I14…

   Promoter

   Available since

   Aug. 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJL-SaKKH-BE3

   Plasmid

   #85170

   Purpose

   Expresses SaKKH-BE3 in mammalian cells [Edit]

   Depositor

   David Liu

   Article

   Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   SaKKH-BE3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3 Flag C/EBPa

   Plasmid

   #66978

   Purpose

   Expression plasmid for mouse C/EBPa; adds Flag tag to the N-terminus of protein

   Depositor

   Christopher Vakoc

   Article

   Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

   Insert

   C/EBP alpha (Cebpa Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-hSyn-eNpHR 3.0-EYFP

   Plasmid

   #26972

   Purpose

   AAV expression of eNpHR 3.0 fused to EYFP driven by humanized Synapsin promoter for optogenetic inhibition

   Depositor

   Karl Deisseroth

   Has Service

   AAV2 and AAV5

   Insert

   eNpHR 3.0
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Trafficking Signal (TS) ER Export Signal

   Promoter

   hSyn

   Available since

   Feb. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAD/myc-his A Rv0712 (FGE)

   Plasmid

   #16132

   Purpose

   Bacterial expression of Mycobacterium tuberculosis FGE

   Depositor

   Carolyn Bertozzi

   Article

   Carrico et al Nat Chem Biol. 2007 Jun . 3(6):321-2.

   Insert

   Formylglycine-generating enzyme
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAV-CAG-hChR2-H134R-tdTomato

   Plasmid

   #28017

   Purpose

   AAV-mediated expression of hChR2(H134R) for neuronal excitation.

   Depositor

   Karel Svoboda

   Article

   Mao et al Neuron. 2011 Oct 6;72(1):111-23.

   Has Service

   AAV Retrograde

   Insert

   humanized ChR2-H134R-tdTomato
   

   Use

   AAV; Adeno associate viral

   Tags

   tdTomato

   Expression

   Mutation

   H134R

   Promoter

   CAG

   Available since

   Nov. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV PRDM16

   Plasmid

   #15504

   Depositor

   Bruce Spiegelman

   Article

   Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

   Insert

   PRDM16 (isoform 4) (Prdm16 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSKBAD2

   Plasmid

   #15335

   Depositor

   Hideo Iwai

   Article

   Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

   Insert

   DnaE C-intein
   

   Use

   Tags

   GB1

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV5 TrkA

   Plasmid

   #15002

   Depositor

   Raymond Birge

   Article

   Yano et al J Neurosci Res. 2000 Feb 1. 59(3):356-64

   Insert

   TrkA (NTRK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V289L

   Promoter

   Available since

   Aug. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-Puro

    Plasmid

    #141368

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp2 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pPAmCherry1-N1

    Plasmid

    #31928

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

    Promoter

    cmv

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMXs-IP spGFP-ERGIC53

    Plasmid

    #38270

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Autophagy. 2010 Aug;6(6):764-76.

    Insert

    ERGIC-53 (LMAN1 Human)
    

    Use

    Retroviral

    Tags

    EGFP with Calreticulin signal peptide at N-termin…

    Expression

    Mammalian

    Mutation

    deleted signal peptide (1-30 amino acids)

    Promoter

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mEos4b-C1

    Plasmid

    #54812

    Purpose

    Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Loren Looger, Michael Davidson

    Article

    Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Type

    Empty backbone

    Use

    Tags

    mEos4b

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. mCerulean C1

    Plasmid

    #27796

    Depositor

    Steven Vogel

    Article

    Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

    Insert

    mCerulean C1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBMN DHFR(DD)-YFP

    Plasmid

    #29325

    Depositor

    Thomas Wandless

    Article

    Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

    Insert

    DHFR(DD)-YFP
    

    Use

    Retroviral

    Tags

    HA and YFP

    Expression

    Mutation

    Change Arg 12 to Tyr (R12Y),Gly 67 to Ser (G67S) …

    Promoter

    Available since

    July 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FUW-Sox10

    Plasmid

    #36978

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    Sox10 (SOX10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO-puro FLAG SREBP1

    Plasmid

    #32017

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    SREBP1 (Srebf1 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    V225M

    Promoter

    CMV

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. gRNA_AAVS1-T1

    Plasmid

    #41817

    Purpose

    Expresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_AAVS1-T1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNCS-mRuby3

    Plasmid

    #74234

    Purpose

    Constitutive expression of mRuby3 in bacteria

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Insert

    mRuby3
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pThy1-Brainbow3.2

    Plasmid

    #45179

    Depositor

    Joshua Sanes

    Article

    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

    Insert

    pThy1-Brainbow3.2
    

    Use

    Linearized for transgenic mouse injection

    Tags

    Expression

    Mutation

    Promoter

    Thy1

    Available since

    Nov. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBAC-BA-lacZ

    Plasmid

    #13423

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    BA
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. IRE1 alpha KA-pcDNA3.EGFP

    Plasmid

    #13010

    Depositor

    Fumihiko Urano

    Article

    Lipson et al Cell Metab. 2006 Sep . 4(3):245-54.

    Insert

    Inositol Requiring Enzyme-1 (ERN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R4L, K599A

    Promoter

    CMV

    Available since

    Nov. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. dxCas(3.7)-VPR

    Plasmid

    #108383

    Purpose

    Mammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminus

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    dxCas(3.7)-VPR
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSECC

    Plasmid

    #60820

    Purpose

    3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre

    Depositor

    Tyler Jacks

    Article

    Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature

    Inserts

    Cas9
    Cre
    

    Use

    CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    BsmBI site eliminated by C->A; E308G

    Promoter

    EFS and EFS (after Cas9-2A)

    Available since

    Nov. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBMN-I-GFP

    Plasmid

    #1736

    Purpose

    This retroviral vector has an IRES-GFP downstream of the cloning sites

    Depositor

    Garry Nolan

    Insert

    IRES-GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-SypHluorin 4x (S4x)

    Plasmid

    #37005

    Depositor

    Stephen Heinemann, Yongling Zhu

    Article

    Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

    Insert

    SypHluorin 4x (Syp Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Four copies of pHluorin inserted at site 184-185

    Promoter

    CMV

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAG-DsRed2

    Plasmid

    #15777

    Depositor

    Edward Callaway

    Article

    Wickersham et al Neuron. 2007 Mar 1. 53(5):639-47.

    Insert

    DsRed2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p2xFlag CMV2-YAP2

    Plasmid

    #19045

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBMPC3

    Plasmid

    #31367

    Depositor

    Aziz Sancar

    Article

    Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

    Insert

    Bmal1 (Bmal1 Mouse)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pIRESneo-FLAG/HA EYFP

    Plasmid

    #10825

    Depositor

    Thomas Tuschl

    Article

    Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

    Insert

    EYFP
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge