We narrowed to 15,661 results for: genome
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pPP089
Plasmid#158796PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purificationDepositorInsertCasPhi-3
ExpressionBacterialAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2R3z-BASI-ccdB-BSAI
Plasmid#118389PurposeEntry clone containing ccdB negative selection marker. Used to clone any DNA fragments of interest; goldengate and gibson assembly cloning compatible. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertccdB
UseUnspecifiedMutationSee Depositor Comments SectionAvailable SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSS9
Plasmid#71655PurposeContains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coliDepositorInsertgfpuv
UseSynthetic BiologyPromoterT7A1Available SinceJan. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSQT834
Plasmid#53371PurposeCsy4 and Cas9 nuclease expression plasmidDepositorInsertCsy4-T2A-Cas9-NLS
UseCRISPRExpressionMammalianPromoterCAGAvailable SinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMCB306-GFP-Rab12
Plasmid#198471Purposelentiviral expression of GFP-Rab12DepositorAvailable SinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_membrane_EGFP
Plasmid#139402Purposein vitro transcription for mRNADepositorInsertmembrane-EGFP
UseIn vitro transcriptionTagsEGFPPromoterT3Available SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBA1015
Plasmid#189589PurposeT7gp1.7 deletion HR vector 250bp HR Arms (T7 phage editing)DepositorInsertT7gp1.7 Locus 250bp Homology Arms
UseSynthetic BiologyExpressionBacterialMutationEncodes for a full deletion of T7gp1.7Available SinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open]
Plasmid#71805PurposeGenerating transgenic flies for 4-hydroxytamoxifen-inducible DamIDDepositorInsertFL.hsp70P-Dam[4-HT-intein@L127C]-Myc[open]
ExpressionInsectAvailable SinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSG4K5
Plasmid#74492PurposeFor cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119Available SinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
psgRNAv1-empty
Plasmid#171611PurposesgRNA_v1 plasmid for AsCas12f1 in bacteriaDepositorInsertAsCas12f1_sgRNA_1
ExpressionBacterialAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11296: pHR-HSP-hyperdCas12a-miniVPR-EPS-puro
Plasmid#210604Purposeheat shock promoter - activated hyperdCas12a-based transcriptional activatorDepositorInserthyperdCas12a-miniVPR
UseCRISPR and LentiviralPromotertruncated hsp70Available SinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
prDNA spacer-LacOx50-20xMS2-Hyg
Plasmid#210219PurposeIntegrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.DepositorInserts50xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865
Available SinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-mRuby3-2A-Cre
Plasmid#203844PurposeFlp-dependent Cre and mRuby3 expressionDepositorInsertCre
UseAAVTagsmRuby3-2AAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvCAHS3-CAHS3-mEGFP
Plasmid#205006PurposeThe vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the RvCAHS3-mEGFP gene positioned in between.DepositorInsertRvCAHS3-mEGFP with 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
UseTardigrade expressionAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-itis pSel
Plasmid#195344PurposeSelection plasmid expressing non-fluorescent EGFP A111VDepositorInsertEGFP
UseCRISPRMutationA111VPromoterJ23119Available SinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvSAHS1-SAHS1-mEGFP
Plasmid#205007PurposeThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the RvSAHS1-mEGFP gene positioned in between.DepositorInsertRvSAHS1-mEGFP with 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
UseTardigrade expressionAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
gRNA NIA Null
Plasmid#198728PurposeExpresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Neither gRNA is fused to a mobility signal and are therefore non-mobile.DepositorInsertAtNIA1 gRNAs
ExpressionPlantPromoterpU6-26, pU6-29Available SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0501
Plasmid#91011PurposeModule A, Promoter: 35S, Gene: Csy4-P2A-AtCas9, Terminator: HSPDepositorInsertCsy4-P2A-AtCas9
UseCRISPRPromoter35SAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
ePE2
Plasmid#202029PurposePrime Editing plasmid for tag insertionDepositorInsertCas9，
UseCRISPRExpressionPlantAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCB036 FoMV-sgGFP
Plasmid#234813PurposeTo express gRNA from Foxtail Mosaic Virus Vector targeting GFP (T-DNA)DepositorInsertgRNA targeting GFP
ExpressionPlantAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE HA-epitope donor ORF1
Plasmid#200372PurposeTagging endogenously expressed proteins with HA epitope, C-terminalDepositorInsertHA epitope
ExpressionMammalianMutationNAAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCE38
Plasmid#174432PurposeC. auris LEUpOUT HYG marker CAS9 expression construct. Use with pCE41 gRNA expression construct.DepositorInsertC. auris LEU2 1 of 2, pENO1, Cas9, HYG 1 of 2
UseCRISPRExpressionBacterialAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCE41
Plasmid#174433PurposeC. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.DepositorInsertHYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
UseCRISPRExpressionBacterial and YeastAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
SaCas9_sgRNA_expression_in_pBluescript
Plasmid#122090PurposeU6 driven SaCas9 sgRNA expression vector for cloning own guidesDepositorAvailable SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA-Sirius-8XPP7
Plasmid#121940PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XPP7
UseCRISPR and LentiviralTagsnoExpressionMammalianPromoterhuman U6 promoterAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
P2A-NLS-tdtomato-EPG backbone
Plasmid#215548PurposeAn empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFPDepositorTypeEmpty backboneUseCRISPRAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA-Sirius-4X(MS2-PP7)
Plasmid#121941PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-4X(MS2-PP7)
UseCRISPR and LentiviralTagsnoExpressionMammalianPromoterhuman U6 promoterAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
1162_pAAV-HLP-EmGFP-SpA
Plasmid#109313PurposePlasmid for AAV EmGFP Mammalian ExpressionDepositorInsertEmGFP
UseAAVExpressionMammalianAvailable SinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ167
Plasmid#69309PurposeGateway entry vector with Cas9pDepositorInsertCas9p (Plant codon-optimized; high GC content at 5 prime region)
UseCRISPRMutationNLS fusionAvailable SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
P2A-mCherry-EhT backbone
Plasmid#215549PurposeAn empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of hygromycin-NLS-tdTomatoDepositorTypeEmpty backboneUseCRISPRAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFSW FLEx mCherry-KASH
Plasmid#139652PurposeConditional expression of mCherry-KASHDepositorInsertmCherry-KASH
ExpressionMammalianPromoterSynapsinAvailable SinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3001
Plasmid#91094PurposeModule C, Promoter: 35S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedPromoter35SAvailable SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTA-HUH
Plasmid#221152PurposeHisG-URA3-HisG recycling type marker in pTAs for Yarrowia lipolyticaDepositorInsertHisG-URA3-HisG
ExpressionBacterial and YeastAvailable SinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF644_2
Plasmid#86306PurposeEncodes gRNA for 3' target of human ZNF644DepositorInsertgRNA against ZNF644 (ZNF644 Human)
UseCRISPRAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF644_1
Plasmid#86305PurposeEncodes gRNA for 3' target of human ZNF644DepositorInsertgRNA against ZNF644 (ZNF644 Human)
UseCRISPRAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1111
Plasmid#91032PurposeModule A, Promoter: OsAct1, Gene: TaCas9, Terminator: HSPDepositorInsertTaCas9
UseCRISPRPromoterOsAct1Available SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti_AID-Dam-only
Plasmid#119765PurposeLentiviral plasmid for Mammalian ExpressionDepositorInsertAID-Dam only
UseLentiviralExpressionMammalianAvailable SinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
WNK2 WT
Plasmid#24569DepositorAvailable SinceMay 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Nat
Plasmid#232097PurposeYeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with NatMX selectionDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only
