163,606 results
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Plasmid#227908PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertRAB14 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML325_GPR107-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227909PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertGPR107 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML323_GOLGA2-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227910PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertGOLGA2 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML494_ATP1B3-C-term_XTEN80-sfGFP-3xHA_pTMPLT
Plasmid#227912PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertATP1B3 homology arms with XTEN80-sfGFP-3xHA for insertion at C terminus
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML499_SLC3A2-C-term_XTEN80-sfGFP-3xHA_pTMPLT
Plasmid#227913PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertSLC3A2 homology arms with XTEN80-sfGFP-3xHA for insertion at C terminus
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML454_G3BP1-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227915PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertG3BP1 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML359_CAPRIN1-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227916PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertCAPRIN1 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML346_VPS35-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227891PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertVPS35 homology arms with mEGFP-3xHA for insertion at C terminus
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML530_EEA1-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227892PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertEEA1 homology arms with mEGFP-3xHA for insertion at N terminus
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML348_ATG101-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227900PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertATG101 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML460_MAP1LC3B-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227901PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertMAP1LC3B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML349_PEX3-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227902PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertPEX3 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML319_SEC31A-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227904PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertSEC31A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML317_COPE-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227905PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertCOPE homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHEE401_GV_MOD
Plasmid#194874PurposemCherry selection in Arabidopsis seeds in T1 and T2DepositorInsertCas9
UseCRISPRTagsExpressionMutationPromoteregg cell-specificAvailable SinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCN38-ktrA-yfp
Plasmid#227636Purposec-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.DepositorInsertktrA-yfp
UseTagsExpressionBacterialMutationPromoterAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM236 Ubc4
Plasmid#226361PurposeExpression of yeast Ubc4DepositorAvailable SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIVEX2.3-Theo-3c-EGFP
Plasmid#217526PurposeTheo-3c riboswitch-regulated GFP reporter. The gene is inserted into downstream of T7 promoter.DepositorInsertTheo-3c riboswitch-regulated EGFP
UseIn vitro transcription-translationTagsExpressionMutationPromoterT7Available SinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cut190*_pET21b
Plasmid#215404PurposeExpression construct for Cut190* gene, codon optimized for expression in E. coliDepositorInsertCut190*
UseTagsExpressionBacterialMutationS226P/R228SPromoterAvailable SinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEB018
Plasmid#215568PurposeFuses insert of interest to an HA-tag, stop codon and S. commune Hom2 terminator, with flanks directing the construct to S. commune targeted integration locusDepositorInsert3 x Hemagglutinin (HA) Tag / Stop codon / Intron / Hom2-terminator
UseFungal homologous recombination plasmidTagsExpressionMutationPromoterAvailable SinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_FKBP-V5-ALX1
Plasmid#215023PurposeAAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human ALX1DepositorAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-VcRibB
Plasmid#187977PurposeExpresses 3,4-dihydroxy-2-butanone 4-phosphate synthase RibB from Vibrio choleraeDepositorInsertVcRibB
UseTagsHexahistagExpressionBacterialMutationPromoterT7Available SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF1046
Plasmid#143748PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-Green Falcan1
Plasmid#199705Purposegreen fluorescent indicator for cAMP imagingDepositorInsertGreen Falcan1
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJBL3907
Plasmid#193140PurposeClostridium beijerinckii pfl ZTP riboswitch with sfGFP reporterDepositorInsertClostridium beijerinckii (Cbe) pfl ZTP riboswitch with sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJBL6090
Plasmid#193142PurposeClostridium beijerinckii pfl ZTP riboswitch A26∆ OFF control with sfGFP reporterDepositorInsertCbe ZTP Riboswitch sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationDeleted A26 to disable ligand-bindingPromoterJ23119Available SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Portal_PhiV1
Plasmid#188811PurposePortal protein from phage PhiV1DepositorInsertPortal
UseTagsExpressionBacterialMutationPromoterAvailable SinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Terminase_ST32
Plasmid#188850PurposeLarge terminase from phage ST32DepositorInsertTerminase
UseTagsExpressionBacterialMutationPromoterAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
EpAvs1
Plasmid#188788PurposeAvs1 from Erwinia piriflorinigrans CFBP5888DepositorInsertEpAvs1
UseTagsExpressionBacterialMutationPromoterblaAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpPLK1-GFP
Plasmid#185573Purposeto see protein dynamicsDepositorInsertPLK1
UseIn vitro transcription (mrna synthesis)TagsGFPExpressionMutationPromoterAvailable SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK19 pCAS-Tyr-[gRNA: 2=FF21] (SplitHygR, AmpR)
Plasmid#179003PurposeSp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC13N-dCas9-BFP-KRAB
Plasmid#127968Purposeconstitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)DepositorInsertCLYBL-CAG-dCas9-NLS-BFP-KRAB
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV.rTH.PI.Cre.SV40
Plasmid#107788PurposeAAV vector expressing Cre from rTH promoterDepositorHas ServiceAAV Retrograde and AAV9InsertCre
UseAAVTagsExpressionMammalianMutationPromoterrTHAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
VSV.G
Plasmid#14888DepositorInsertVSV-G Envelope
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
lenti-EF1a-dCas9-VP64-Puro
Plasmid#99371Purpose3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)DepositorInsertdCas9-VP64-T2A-Puro
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Blasto
Plasmid#167904PurposeLentiviral vector for expressing U6 sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
p65-mNeonGreen
Plasmid#127172PurposeFluorescently tagged p65 reporterDepositorInsertGFP-RelA (RELA Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable SinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
PEP101E
Plasmid#97387Purposebinary construct of cytosolic sfGFP1-10 for plantDepositorInsertsfGFP1-10OPT
UseTagsExpressionPlantMutationPromoterAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
RpH-ILV-3xALFA
Plasmid#225135PurposeRpH-ILV probe strongly colocalises with lysosomal markers.DepositorAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEvol-MjaYRS
Plasmid#153557PurposeAmber suppression for incorporating 3-aminotyrosine to proteins in E. coliDepositorInsertsMJaYRS (first copy)
MJaYRS (second copy)
amber suppression tRNA under proK promoter
UseTagsExpressionBacterialMutationPromoterglnS and pBADAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only