179,521 results

Showing: 4641 - 4680 of 179521 results

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pAAV-CaMKIIa-eArch 3.0-EYFP

Plasmid

#35516

Purpose

AAV expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

Depositor

Karl Deisseroth

Publication

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

Arch3.0-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Enhanced trafficking signal and ER export signal …

Promoter

CaMKIIa

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pET His6 Thioredoxin TEV LIC cloning vector (2T-T)

Plasmid

#29712

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6-Thioredoxin-TEV

Expression

Bacterial

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CALM-pmCherryN1

Plasmid

#27691

Depositor

Christien Merrifield

Publication

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CALM (Picalm Mouse)

Tags

mCherry

Expression

Mammalian

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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hWIP1 FLAG

Plasmid

#28105

Depositor

Lawrence Donehower

Publication

Moon et al J Biol Chem. 2010 Apr 23. 285(17):12935-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

WIP1 (PPM1D Human)

Tags

FLAG

Expression

Mammalian

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCMVTNT-T7-KPNA1

Plasmid

#26677

Depositor

Bryce Paschal

Publication

Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Karyopherin Alpha 1 (KPNA1 Human)

Tags

T7

Expression

Mammalian

Mutation

KPNA1 sequence contains a reported SNP at S73N

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pDONR223_BFP

Plasmid

#25891

Purpose

Gateway entry vector containing BFP.

Depositor

David Root

Publication

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

BFP

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223-MERTK

Plasmid

#23900

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MERTK (MERTK Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pDEST51-LRRK2-WT

Plasmid

#25080

Depositor

Mark Cookson

Publication

J Biol Chem. 2008 Jun 13;283(24):16906-1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LRRK2 (LRRK2 Human)

Use

Gateway Cloning

Tags

6XHis and V5

Expression

Mammalian

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGBMCS-SortA

Plasmid

#21931

Depositor

Fuyuhiko Inagaki

Publication

Kobashigawa et al J Biomol NMR. 2009 Mar . 43(3):145-50.

Insert

sortase (srtA Staphylococcus aureus (strain N315))

Tags

GB1 and His-tag

Expression

Bacterial

Mutation

deleted amino acids 1-59

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles
CHOP::GFP

Plasmid

#21898

Depositor

David Ron

Publication

Novoa et al J Cell Biol. 2001 May 28. 153(5):1011-22

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CHOP (Ddit3 Mouse)

Use

Non-viral

Tags

EGFP

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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mEGFP-HRas S17N

Plasmid

#18665

Depositor

Karel Svoboda

Publication

Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H-Ras (HRAS Human)

Tags

monomeric EGFP

Expression

Mammalian

Mutation

S17N mutation in H-Ras

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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PUMA Frag1-Luc

Plasmid

#16591

Depositor

Bert Vogelstein

Publication

Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PUMA promotor fragment (BBC3 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pcDNA3 barr1 flag

Plasmid

#14687

Depositor

Robert Lefkowitz

Publication

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

beta arrestin 1 (Arrb1 Rat)

Tags

flag

Expression

Mammalian

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles
pBS human c-Myc

Plasmid

#14971

Depositor

Joan Massague

Publication

Siegel et al J Biol Chem. 2003 Sep 12. 278(37):35444-

Insert

c-Myc (MYC Human)

Expression

Bacterial

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Hmga2 3'UTR wt luciferase (Luc-wt)

Plasmid

#14785

Depositor

David Bartel

Publication

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Luc-wt (Hmga2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pEP PolI

Plasmid

#11722

Depositor

Lawrence Loeb

Publication

Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

Insert

DNA polymerase I EP (polA E. coli)

Expression

Bacterial

Mutation

D424A, I709N, A759R. Error-prone version of PolI.

Available since

Aug. 8, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFLAG/HA-MOV10

Plasmid

#10976

Depositor

Thomas Tuschl

Publication

Meister et al Curr Biol. 2005 Dec 6;15(23):2149-55. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MOV10 (MOV10 Human)

Tags

FLAG and HA

Expression

Mammalian

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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EGFR G719S

Plasmid

#11013

Purpose

Retroviral construct for expressing human EGFR with G719S mutation in human cells

Depositor

Matthew Meyerson

Publication

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR G719S (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

G719S

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pCMV-evoCAST-eCas8

Plasmid

#234727

Purpose

Engineered human-codon optimized PseCas8 in mammalian cells (for evoCAST)

Depositor

David Liu

Publication

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ePseCas8

Expression

Mammalian

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-evoCAST-TniQ

Plasmid

#234728

Purpose

Engineered human-codon optimized PseTniQ in mammalian cells (for evoCAST)

Depositor

David Liu

Publication

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PseTniQ

Expression

Mammalian

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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PiggyBac PlayBack

Plasmid

#203312

Purpose

To all for PiggyBac gene insertion into Genome

Depositor

Aleksandar Necakov

Publication

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pJRH-1180 U6-reci Gag-pol v2

Plasmid

#201915

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Publication

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-pol

Expression

Mammalian

Promoter

CAG

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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lentiCRISPRi(v2)-Blast

Plasmid

#170068

Purpose

Expresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistance

Depositor

Neville Sanjana

Publication

Morris et al Science. 2023 May 19;380(6646):eadh7699.

Insert

KRAB-dCas9-MeCP2

Use

CRISPR and Lentiviral

Tags

2A and 2xHA tag

Expression

Mammalian

Promoter

EFS-NS

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pK18msB

Plasmid

#177839

Purpose

Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size.

Depositor

Christopher Johnson

Publication

Ling et al Nat Commun. 2022 Aug 22;13(1):4925. doi:

Type

Empty backbone

Use

Bacterial gene replacement

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PB-TO-NFIA.1-SOX9

Plasmid

#182310

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

NFIA-T2A-SOX9 (NFIA, SOX9 Human)
puroR-T2A-mycNLS-mTagBFP2

Use

Piggybac

Tags

None and T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

EF1a and TRE3G

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-AsCas12f1

Plasmid

#171614

Purpose

AsCas12f1-based Human cell genome editing

Depositor

Quanjiang Ji

Publication

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AsCas12f1 and sgRNA_v1

Expression

Mammalian

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
p-EF1a-CreERT2-3Xflag-T2A-eBFP2

Plasmid

#170186

Purpose

This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing

Depositor

Bradley Bernstein

Publication

Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:

Insert

CreERT2-T2A-eBFP2

Use

Lentiviral

Promoter

EF1 alpha

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAR-Ec633

Plasmid

#130873

Purpose

A nuclear plasmid encoding an error-prone mutant TP-DNAP1 (L477V, L640Y, I777K, W814N) for OrthoRep

Depositor

Chang Liu

Publication

Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

Insert

TP-DNAP1

Expression

Yeast

Mutation

L477V, L640Y, I777K, W814N

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBT281

Plasmid

#122611

Purpose

express evoAPOBEC1-BE4max in mammalian cells

Depositor

David Liu

Publication

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

evoAPOBEC1-BE4max

Expression

Mammalian

Mutation

E4K H109N H122L D124N R154H A165S P201S F205S

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMIH-TFAM-GFP

Plasmid

#113704

Purpose

Fluorescent fusion protein used to visualise mitochondrial nucleoids, with hygromycin selection

Depositor

Benjamin Kile

Publication

McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

Insert

TFAM (Tfam Mouse)

Use

Retroviral

Tags

eGFP

Expression

Mammalian

Promoter

MSCV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMXs GFP-LC3-RFP

Plasmid

#117413

Purpose

Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.

Depositor

Noboru Mizushima

Publication

Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

EGFP and mRFP1

Expression

Mammalian

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pAAV-MBP-2xNLS-tdTomato

Plasmid

#104054

Purpose

An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

Depositor

Viviana Gradinaru

Publication

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

2xNLS-tdTomato

Use

AAV

Tags

NLS

Promoter

MBP

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHR-Tre3G-29xGGGGCC-12xMS2

Plasmid

#99149

Purpose

Encodes 29xGGGGCC repeats with 12xMS2 tag, 2nd generation lentiviral vector. *Please see comments section below about the number of verified repeats.

Depositor

Ron Vale

Publication

Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

Insert

29xGGGGCC 12xMS2 hairpins

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3-N-Flag-Caspase-1

Plasmid

#75128

Purpose

Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

Depositor

Bruce Beutler

Publication

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

caspase 1 (Casp1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

P341S in Caspase-1 (please see depositor comments…

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pLPCX2-MT1-MMP-eGFP

Plasmid

#89819

Purpose

Express in MT1-MMP mammalian cells

Depositor

Shengyu Yang

Publication

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

MT1-MMP (MMP14 Human)

Use

Retroviral

Tags

eGFP

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6.2-Nanoluc-ccdB

Plasmid

#87078

Purpose

Lentiviral Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferase

Depositor

Mikko Taipale

Type

Empty backbone

Use

Lentiviral

Tags

Nanoluc luciferase

Expression

Mammalian

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

Enlarge
LentiCRISPRv2Cre

Plasmid

#82415

Purpose

Lentiviral vector expressing Cre recombinase alongside Cas9 and an sgRNA cloning site

Depositor

David Feldser

Publication

Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

Insert

Cre Recombinase

Use

CRISPR, Cre/Lox, and Lentiviral

Mutation

Mutated BsmBI cut site

Promoter

EFS (P2A)

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

Enlarge
pLentiCRISPR-mCherry

Plasmid

#75161

Purpose

Derived from LentiCRISPR v1 but expresses mCherry instead of puromycin resistance. 3rd generation.

Depositor

Beat Bornhauser

Publication

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

Enlarge
pcDNA3.1+ hnRNPK myc HIS

Plasmid

#71662

Purpose

Mammalian expression of hnRNPK

Depositor

Gideon Dreyfuss

Insert

hnRNPK (HNRNPK Human)

Tags

HIS and myc

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
pUC18T-mini-Tn7T-Tp-gfpmut3

Plasmid

#65033

Purpose

TpR mini-Tn7 vector, mobilizable, for GFP tagging bacteria

Depositor

Herbert Schweizer

Publication

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

gfpmut3

Expression

Bacterial

Available since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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179,521 results