165,965 results

1. AAV-U6-sgRNA-EF1α-sakkhCas9-HA-NLS-polyA-CBh-EGFP-polyA

   Plasmid

   #168305

   Purpose

   mediated knock-in of sgRNA precursor

   Depositor

   Hui Yang

   Article

   Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

   Insert

   SaKKH-EGFP-U6-sgRNA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   EF1alpha, CBh

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW57-GFP-2A-MCS

   Plasmid

   #71783

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Expression

   Mammalian

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. GSK3beta  (1015)

   Plasmid

   #49491

   Purpose

   expresses human Gsk3beta

   Depositor

   Scott Friedman

   Article

   Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

   Insert

   GSK3beta (GSK3B Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSRK2706

   Plasmid

   #78571

   Purpose

   Expresses untagged human rhinovirus 3C protease in E. coli

   Depositor

   David Waugh

   Article

   Raran-Kurussi et al Anal Biochem. 2016 Apr 19;504:30-37. doi

   Insert

   human rhinovirus 3C protease (HRVBgp1 Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMT525

   Plasmid

   #131392

   Purpose

   L1RP retrotransposition reporter

   Depositor

   Kathleen Burns

   Article

   Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

   Insert

   L1RP GFP-AI
   

   Expression

   Mammalian

   Available Since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-EF1a-GFP-LaminA-IRES-Hygromycin

   Plasmid

   #134867

   Purpose

   Expresses EGFP-tagged nuclear envelope marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Lamin A (LMNA Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. HcKCR2_mCherry_pcDNA3.1

   Plasmid

   #177337

   Purpose

   Expresses KCR2 in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

   Insert

   HcKCR2
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   mammalian codon-adapted

   Available Since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPR::rpsL

   Plasmid

   #44505

   Purpose

   A crRNA expression plasmid specific to the rpsL allele.

   Depositor

   Luciano Marraffini

   Article

   Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   CRISPR::rpsL (rpsL )
   

   Use

   CRISPR; E.coli

   Available Since

   April 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. All_in_one_CRISPR/Cas9_LacZ

   Plasmid

   #74293

   Purpose

   "All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening

   Depositor

   Lynne Postovit

   Article

   Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do

   Inserts

   LacZ-alpha
   Cas9
   mCherry
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Promoter

   Lac promoter, SV40, and T7 promoter

   Available Since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. 18065-M01-412

    Plasmid

    #225664

    Purpose

    Lentiviral expression of FLAG-tagged fluorescent proteins for immunohistochemical detection in FFPE tissue

    Depositor

    Dominic Esposito

    Article

    Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

    Inserts

    dsCopGFP
    delCD19 (CD19 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Mutation

    C-terminally truncated (aa 1-333 only)

    Promoter

    SORE6-mCMVp and WPRE-SV40p

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. Hsp90 HA

    Plasmid

    #22487

    Depositor

    William Sessa

    Article

    Garcia-Cardena et al Nature. 1998 Apr 23. 392(6678):821-4.

    Insert

    Hsp90 HA (HSP90AB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRC-CMV-Rev1b

    Plasmid

    #164443

    Purpose

    Encodes HIV-1 Rev accessory protein for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    HIV-1 Rev
    

    Expression

    Mammalian

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Anti-VGAT [L118/80R]

    Plasmid

    #177463

    Purpose

    Mammalian Expression Plasmid of anti-VGAT (Mouse). Derived from hybridoma L118/80.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-VGAT (Mus musculus) recombinant mouse monoclonal antibody (Slc32a1 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. HiBiT-SRPK1 Fusion Vector

    Plasmid

    #236908

    Purpose

    Express HiBiT-SRPK1 in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    SRPK1 (SRPK1 Human)
    

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pEGFP-FRB

    Plasmid

    #25919

    Depositor

    Klaus Hahn

    Article

    Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

    Insert

    FRB (MTOR Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBAC-lacZ

    Plasmid

    #13422

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox

    Expression

    Bacterial

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-phSyn-flex-SypEGFP

    Plasmid

    #153203

    Purpose

    Can be used to generate AAV virus that will mark the presynaptic terminal (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

    Depositor

    Lynette Lim

    Insert

    synaptophysin-EGFP
    

    Use

    AAV

    Promoter

    rat synapsin

    Available Since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-fNPY-GFP

    Plasmid

    #22912

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    fugu neuropeptide Y promoter driving GFP
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-C1-TULP3

    Plasmid

    #218741

    Purpose

    Expresses C-terminally EGFP-tagged TULP3 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Insert

    TULP3 (TULP3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

    Plasmid

    #185914

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. GRK2-K220R

    Plasmid

    #35403

    Depositor

    Robert Lefkowitz

    Article

    Freedman et al J Biol Chem. 1995 Jul 28;270(30):17953-6

    Insert

    GRK2 (GRK2 Bovine)
    

    Expression

    Mammalian

    Mutation

    K220R

    Promoter

    CMV

    Available Since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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22. Ubc.Luc.IRES.Puro

    Plasmid

    #33307

    Depositor

    Linzhao Cheng

    Article

    Jang et al Mol Imaging. 2011 Apr;10(2):111-22.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    Ubc

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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23. pDAC627

    Plasmid

    #195240

    Purpose

    Mammalian expression of Csm complex from separate promoters; Puro backbone (Used for RNA knockdown)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; Puro
    

    Expression

    Mammalian

    Available Since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. mEGFP-C1

    Plasmid

    #54759

    Purpose

    Localization: C1 Cloning Vector, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pAAV-hSyn-GRAB_ACh3.0

    Plasmid

    #121922

    Purpose

    express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0

    Depositor

    Yulong Li

    Article

    Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

    Has Service

    AAV9

    Insert

    GPCR activation based ACh sensor GRAB-ACh3.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pET-28a_6H-TAQ_E602D

    Plasmid

    #166944

    Purpose

    Bacterial expression of Taq DNA polymerase

    Depositor

    Robert Tjian

    Article

    Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

    Insert

    Thermus aquaticus DNA polymerase
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    E602D

    Promoter

    T7

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. mCherry-Vimentin-7

    Plasmid

    #55156

    Purpose

    Localization: Intermediate Filaments, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Article

    Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

    Insert

    Vimentin (VIM Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

28. TurboID-V5_pRS415

    Plasmid

    #107167

    Purpose

    expresses V5-tagged TurboID in the yeast cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Expression

    Yeast

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. LentiCRISPRv2-Beclin1

    Plasmid

    #99574

    Purpose

    Expressed Cas9 with sgRNA targeting Beclin1

    Depositor

    Edward Campbell

    Article

    Imam et al J Virol. 2016 Jan 13. pii: JVI.03033-15.

    Insert

    gRNA targeting Beclin1 (BECN1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEGFP-Sec24D

    Plasmid

    #32678

    Depositor

    Henry Lester

    Article

    Richards et al J Biol Chem. 2011 Sep 9;286(36):31241-9.

    Insert

    Sec24D (SEC24D Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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