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171,548 results

Showing: 3511 - 3540 of 171548 results

  2. pGES401

    Plasmid
    #190198
    Purpose
    For CRISPR-Cas9 mediated multiple gene editing in soybean, single transcript unit system driven by soybean elongation factor 1A promoter (pM4), Basta selection
    Depositor
    Yuefeng Guan
    Article
    Bai et al Mol Plant. 2022 Jul 4;15(7):1081-1083. d
    Insert
    spCas9
    Use
    CRISPR
    Tags
    3xFlag
    Expression
    Plant
    Mutation
    plant-codon optimized
    Promoter
    Glycine max elongation factor 1A(pM4)
    Available Since
    Sept. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    190198-plasmid-map-sequence-id-371936

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  4. pBbB8k-csg-NbGFP

    Plasmid
    #166858
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.
    Depositor
    Neel Joshi
    Article
    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469
    Insert
    csgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166858-plasmid-map-sequence-id-329027

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  6. lentiSAMv2

    Plasmid
    #75112
    Purpose
    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    75112-plasmid-map-sequence-id-305764

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  9. pAAV.CMV.PI.EGFP.WPRE.bGH

    Plasmid
    #105530
    Purpose
    AAV expression of EGFP from CMV promoter
    Depositor
    James M. Wilson
    Has Service
    AAV PHP.eB, AAV rh10, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105530-plasmid-map-sequence-id-454420

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  10. pTUB1:OsTIR1-3FLAG, SAG1:CAT

    Plasmid
    #87258
    Purpose
    TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.
    Depositor
    David Sibley
    Article
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Inserts
    TIR1
    CAT
    Use
    Toxoplasma expression
    Tags
    3FLAG
    Mutation
    Codon optimized for Toxoplasma gondii expression
    Promoter
    TgSAG1 361 bp and TgTUB1 2717 bp
    Available Since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87258-plasmid-map-sequence-id-176556

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  12. pAAV-hSyn-DIO-hM3D(Gq)-mCherry

    Plasmid
    #44361
    Purpose
    Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter.
    Depositor
    Bryan Roth
    Article
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM3D(Gq)-mCherry
    Use
    AAV; Adeno associated viral vector
    Tags
    mCherry
    Promoter
    human Synapsin 1
    Available Since
    May 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    44361-plasmid-map-sequence-id-148598

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  20. Inducible Caspex expression

    Plasmid
    #97421
    Purpose
    For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics
    Depositor
    Steven Carr, Samuel Myers
    Article
    Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4
    Inserts
    Caspex
    T2A-Gfp
    Use
    CRISPR
    Tags
    3xFLAG and V5
    Expression
    Mammalian
    Mutation
    dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …
    Promoter
    TRE3G
    Available Since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    97421-plasmid-map-sequence-id-466895

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  23. pLIX_403

    Plasmid
    #41395
    Purpose
    Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA
    Depositor
    David Root
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector, doxycycli…
    Tags
    V5
    Expression
    Mammalian
    Promoter
    TRE promoter, Tet ON
    Available Since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41395-plasmid-map-sequence-id-364439

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  29. AiP15646: pAAV-AiE1426m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN5646)

    Plasmid
    #230884
    Purpose
    AiE1426m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical and striatal Sst-Chodl neurons
    Depositor
    Allen Institute for Brain Science, Jonathan Ting
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Has Service
    AAV2
    Insert
    SYFP2
    Use
    AAV
    Expression
    Mammalian
    Available Since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    230884-plasmid-map-sequence-id-459521

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Showing: 3511 - 3540 of 171548 results