19,585 results

1. pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #191797

   Purpose

   vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized dCas13d
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   R239A, H244A, R858A, H863A

   Promoter

   CBh, SV40, hU6

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME-GAL4-ERT2-VP16

   Plasmid

   #82588

   Purpose

   Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression

   Depositor

   Kryn Stankunas

   Article

   Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

   Insert

   GAL4-ERT2-VP16
   

   Promoter

   None

   Available Since

   Dec. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. eGFP-ova-C1

   Plasmid

   #163524

   Purpose

   Expresses both eGFP and chicken ovalbumin in mammalian cells

   Depositor

   Thomas Weber

   Insert

   enhanced-GFP-chicken ovalbumin tandem
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-PBZ-mRuby2

   Plasmid

   #110650

   Purpose

   expresses a PAR-binding (PBZ) domain fused to mRuby2

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

   Insert

   PBZ domain from APLF, amino acids 371-451 (APLF Human)
   

   Tags

   mRuby2

   Expression

   Mammalian

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiGuide-puro-NLS-GFP

   Plasmid

   #185473

   Purpose

   Mammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCMV-PE2-P2A-hMLH1dn

   Plasmid

   #174827

   Purpose

   Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PE2-P2A-hMLH1dn
   

   Tags

   SV40 bpNLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript; deletion of residues 754-…

   Promoter

   CMV

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-N1_hTRIM32

   Plasmid

   #69541

   Purpose

   Expresses human TRIM32 fused to c-terminal EGFP

   Depositor

   Martin Dorf

   Article

   Fu et al PLoS Pathog. 2015 Jun 9;11(6):e1004960.

   Insert

   tripartite motif containing 32 (TRIM32 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)

   Plasmid

   #85006

   Purpose

   For Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   EGFP-ires-tTA
   

   Expression

   Mammalian

   Available Since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. tetO-ASCL1-LMX1b-NURR1-PuroR

   Plasmid

   #182298

   Purpose

   Doxycycline-inducible vector expressing the ALN transcription factors and PuroR (all separated by 2A sequences)

   Depositor

   Kristen Brennand

   Article

   Powell et al Mol Psychiatry. 2021 Sep 7. pii: 10.1038

   Insert

   ASCL1-2A-LMX1B-2A-NURR1 (ASCL1, LMX1B Human)
   

   Use

   Lentiviral

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-HP1beta

    Plasmid

    #17651

    Depositor

    Tom Misteli

    Article

    Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

    Insert

    HP1 beta (CBX1 Human)
    

    Tags

    GFP and His

    Expression

    Mammalian

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSL2680

    Plasmid

    #85581

    Purpose

    Replicating base vector for constructing cpf1/CRISPR editing plasmids

    Depositor

    Himadri Pakrasi

    Article

    Ungerer et al Sci Rep. 2016 Dec 21;6:39681. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-HP1gamma

    Plasmid

    #17650

    Depositor

    Tom Misteli

    Article

    Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

    Insert

    HP1 gamma (CBX3 Human)
    

    Tags

    GFP and His

    Expression

    Mammalian

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-MH6-uvsY

    Plasmid

    #163914

    Purpose

    Expresses uvsY for bacterial expression and affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    uvsY (uvsY Enterobacteria phage T4)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-uvsX-H6

    Plasmid

    #163913

    Purpose

    Expresses uvsX for bacterial expression and affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    uvsX
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. piRFP-N3-Tyrosinase

    Plasmid

    #80152

    Purpose

    Melanosome marker

    Depositor

    Santiago Di Pietro

    Article

    Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

    Insert

    Tyrosinase (TYR Human)
    

    Tags

    iRFP682

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pSLIK-IDH1-R132H-FLAG

    Plasmid

    #66803

    Purpose

    Tet-inducible expression of mutant IDH1 in mammalian cells, 3rd generation lentiviral vector

    Depositor

    Christian Metallo

    Article

    Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

    Insert

    IDH1 (IDH1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R132H

    Promoter

    CMV

    Available Since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX307-DPP4-puro

    Plasmid

    #158451

    Purpose

    Constitutive lentiviral expression of human DPP4.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    DPP4 (DPP4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBoBi-hLAMP2-C-GC6s

    Plasmid

    #154151

    Purpose

    To detect the calcium ion releases close to the lysosomal surface

    Depositor

    Sheng-cai Lin

    Article

    Li et al Cell Metab. 2019 Sep 3;30(3):508-524.e12

    Insert

    LAMP2 and GCamp6s (LAMP2 Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. mEmerald-Mito-7

    Plasmid

    #54160

    Purpose

    Localization: Mitochondria, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

    Insert

    Mito (COX8A Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pSLIK-IDH1-FLAG

    Plasmid

    #66802

    Purpose

    Tet-inducible expression of IDH1 in mammalian cells, 3rd generation lentiviral vector

    Depositor

    Christian Metallo

    Article

    Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

    Insert

    IDH1 (IDH1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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