161,398 results

1. mKeima-Red-Mito-7

   Plasmid

   #56018

   Purpose

   Localization: Mitochondria, Excitation: 440, Emission: 620

   Depositor

   Michael Davidson

   Insert

   Mito (COX8A Human)
   

   Use

   Tags

   mKeima-Red

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRetrosuper Myc shRNA

   Plasmid

   #15662

   Depositor

   Martin Eilers

   Article

   Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

   Insert

   Myc shRNA (MYC Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pCF159_BRL

   Plasmid

   #225962

   Purpose

   CMV-Intron-BRL (env protein). Expresses BRL (BaEVRLess) env for VLP production.

   Depositor

   Christof Fellmann

   Article

   Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

   Insert

   CMV-Intron-BRL (env protein)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV(CAT)T7-SB100

   Plasmid

   #34879

   Depositor

   Zsuzsanna Izsvak

   Article

   Mates et al Nat Genet. 2009 Jun;41(6):753-61. Epub 2

   Insert

   SB100X transposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX-4T MDM2 WT

   Plasmid

   #16237

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

   Insert

   MDM2 (MDM2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-Cyto-iPASnFR-H-mScarlet3-3xFLAG

   Plasmid

   #225241

   Purpose

   Mammalian expression of iPASnFR-H in the cytosol

   Depositor

   Loren Looger

   Article

   Tamura et al bioRxiv 2024.08.21.609037

   Insert

   iPASnFR-H
   

   Use

   Tags

   3xFLAG and mScarlet3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mCherry

   Plasmid

   #176016

   Purpose

   Mammalian expression of mCherry. Parton lab clone DFQ

   Depositor

   Rob Parton

   Article

   Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-NLS-iPASnFR-H-mScarlet3-3xFLAG

   Plasmid

   #225245

   Purpose

   Mammalian expression of iPASnFR-H in the nucleus

   Depositor

   Loren Looger

   Article

   Tamura et al bioRxiv 2024.08.21.609037

   Insert

   iPASnFR-H
   

   Use

   Tags

   3xFLAG and mScarlet3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV2/6 v1

   Plasmid

   #240485

   Purpose

   AAV packaging plasmid expressing AAV2 Rep protein and AAV6 capsid protein

   Depositor

   Addgene Research Program, Meghan Rego

   Insert

   AAV2 Rep and AAV6 Cap
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pCMV-PE6d

    Plasmid

    #207854

    Purpose

    Mammalian expression of PE6d prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6d
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    M-MLVRTDRNAseHrt(T128N, V223Y, D200C)

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBOB-EF1-FastFUCCI-Puro

    Plasmid

    #86849

    Purpose

    3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection

    Depositor

    Kevin Brindle, Duncan Jodrell

    Article

    Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164

    Inserts

    mKO2-hCDT1(30-120)
    mAG-hGEM(1-110)
    pac (puromycin N-acetyl-transferase)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1 and PGK

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti CMV GFP Puro (658-5)

    Plasmid

    #17448

    Purpose

    3rd gen lentiviral eGFP expression vector, CMV promoter, Puro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSF1389

    Plasmid

    #104962

    Purpose

    Bacterial expression plasmid of bdSENP1 protease

    Depositor

    Dirk Görlich

    Article

    Frey et al J Chromatogr A. 2014 Apr 11;1337:95-105.

    Insert

    SENP1
    

    Use

    Tags

    His14 tag and Tev protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Jan. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLVX-UbC-rtTA-Ngn2:2A:EGFP

    Plasmid

    #127288

    Purpose

    all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)

    Depositor

    Fred Gage

    Article

    Schafer et al Nat Neurosci. 2019 Feb;22(2):243-255. do

    Insert

    Ngn2:2A:Ascl1 (NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    Ngn2 and EGFP linked via 2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL3 Basic Vector

    Plasmid

    #212936

    Purpose

    Firefly luciferase vector for investigating regions controlling transcription

    Depositor

    Debrya Groskreutz

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pCMV-PE6c

    Plasmid

    #207853

    Purpose

    Mammalian expression of PE6c prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6c
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MCP-mCherry

    Plasmid

    #207668

    Purpose

    To express fluorescently labeled MS2 coat binding protein (MCP)

    Depositor

    Christine Mayr

    Article

    Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

    Insert

    MCP-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-parkin WT

    Plasmid

    #45875

    Depositor

    Edward Fon

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin (PRKN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-sgRNA

    Plasmid

    #71409

    Purpose

    There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI

    Depositor

    Eric Lander, David Sabatini

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYC-C

    Plasmid

    #207114

    Purpose

    HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

    Depositor

    Yifan Cheng

    Article

    Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge