166,224 results

1. p103_gRNA_Sp_mClover

   Plasmid

   #213779

   Purpose

   Cloning vector for Sp gRNA, contains Sp gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and GFP for mammalian screening.

   Depositor

   Howard Chang

   Article

   Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   Sp gRNA
   

   Use

   CRISPR and Lentiviral

   Promoter

   mU6

   Available Since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-NL1B

   Plasmid

   #15261

   Depositor

   Peter Scheiffele

   Article

   Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

   Insert

   Neuroligin 1 with insert B (Nlgn1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Jan. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEZYmyc-His

   Plasmid

   #18701

   Purpose

   pcDNA3.1(-)/Myc-His B converted Gateway Destination vector for expressing C-terminal myc-His tagged fusion proteins in mammalian cells

   Depositor

   Yu-Zhu Zhang

   Article

   Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

   Type

   Empty backbone

   Tags

   Myc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 26, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0; cmlc2:EGFP

   Plasmid

   #230075

   Purpose

   Partial ablation using NTR2 and subsequent lineage tracing of regenerating hepatocytes in zebrafish.

   Depositor

   Sumeet Pal Singh

   Article

   Hovhannisyan et al bioRxiv 2025.05.23.655316

   Insert

   lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0
   

   Use

   Cre/Lox; Zebrafish expression

   Promoter

   fabp10a

   Available Since

   May 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBJ2

   Plasmid

   #167131

   Purpose

   A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

   Depositor

   Akira Mine

   Article

   Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

   Insert

   luxCDABE
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   Kanamycin promoter

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHM066

   Plasmid

   #225524

   Purpose

   Encodes Wnt-responsive destablized iRFP

   Depositor

   Jared Toettcher

   Article

   McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

   Insert

   iRFP
   

   Expression

   Mammalian

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE

   Plasmid

   #89609

   Purpose

   over-expression of VEGF-A(165) in human cells

   Depositor

   Fernando Fierro

   Insert

   VEGF-A(165) (VEGFA Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   K188E

   Promoter

   MNDU3

   Available Since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHM126

   Plasmid

   #225528

   Purpose

   Encodes Wnt-responsive destablized GFP

   Depositor

   Jared Toettcher

   Article

   McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

   Insert

   GFP
   

   Expression

   Mammalian

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRE118

   Plasmid

   #43830

   Depositor

   Dieter Schifferli

   Article

   Edwards et al Gene. 1998 Jan 30;207(2):149-57.

   Type

   Empty backbone

   Use

   Bacterial allelic exchange vector with sacb1

   Expression

   Bacterial

   Available Since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSL103-SpCas9-FLAG-3XNLS

    Plasmid

    #182032

    Purpose

    Expression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNP

    Depositor

    Steven Lin

    Article

    Lin et al Bio Protoc. 2022 May 20;12(10):e4420. do

    Insert

    Streptococcus pyogenes cas9 (cas9 Synthetic)
    

    Use

    CRISPR

    Tags

    12X His tag, 3XNLS, FLAG, and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-HA AKT1

    Plasmid

    #78778

    Purpose

    To overexpress AKT1 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

    Insert

    AKT1 (AKT1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 - HA-KLF4 FL

    Plasmid

    #34593

    Depositor

    Michael Ruppert

    Article

    Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

    Insert

    KLF4 (KLF4 Human)
    

    Tags

    HA tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAX APLP1

    Plasmid

    #30141

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Like Protein 1 (APLP1 Human)
    

    Expression

    Mammalian

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pCAG cyto-RCaMP1h

    Plasmid

    #105014

    Purpose

    cytosolic calcium sensor

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

    Insert

    RCaMP1h
    

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-Flag-PGC-1alpha1

    Plasmid

    #45501

    Depositor

    Jorge Ruas, Bruce Spiegelman

    Article

    Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

    Insert

    PGC-1alpha Isoform1 (Ppargc1a Mouse)
    

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Q339K relative to NM_008904

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRE-BI-hGRAD

    Plasmid

    #207837

    Purpose

    Inducible hGRAD Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

    Depositor

    Michaela Mueller-McNicoll

    Article

    Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

    Inserts

    mCherry
    hFBXW11 (FBXW11 Human)
    VHH-GFP4
    

    Expression

    Mammalian

    Mutation

    215 amino acids of the full length protein

    Promoter

    TRE3G BI promoter

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET303_sumocodo_GFP_codoopti

    Plasmid

    #210393

    Purpose

    Express SUMO-GFP in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    GFP
    

    Tags

    SUMO-tag

    Expression

    Bacterial

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pSSRB070_pAC8-hsCRBN

    Plasmid

    #218789

    Purpose

    Insect cell expression vector for FLAG-TEV-Spy-hsCRBN

    Depositor

    Eric Fischer

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    Protein cereblon (CRBN Human)
    

    Tags

    Flag-TEV-Spy

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. U6-BbsI-GGsite-mCherry-Puro

    Plasmid

    #221232

    Purpose

    Cloning vector for sgRNA with mCherry and Puro

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-BEC enhancer-minBG-Cre

    Plasmid

    #236724

    Purpose

    Cre Brain Endothelial Cell Enhancer

    Depositor

    Xiangmin Xu

    Article

    Velazquez-Rivera et al Neuron. 2025 May 21;113(10):1562-1578.e6

    Inserts

    cis-regulatory elements 89763
    Cre Recombinase
    

    Use

    AAV

    Available Since

    July 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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