165,947 results

1. SV-ABAactDA

   Plasmid

   #38247

   Depositor

   Jerry Crabtree

   Article

   Liang et al Sci Signal. 2011 Mar 15;4(164):rs2.

   Inserts

   VP16/PYL1
   Gal4DBD/ABI*
   

   Tags

   Flag, Gal4DBD, HA, and VP16

   Expression

   Mammalian

   Mutation

   D143A

   Promoter

   SV40 (IRES) and SV40p

   Available Since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBabe-puro Ras V12

   Plasmid

   #1768

   Depositor

   Bob Weinberg

   Insert

   ras V12 (HRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   G12V, constitutively active

   Available Since

   June 3, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFV2-HA

   Plasmid

   #187082

   Purpose

   Expression of ATP synthase, with all Cys changed to Ala, and subunit a-HA tagged, subunit beta-His tagged)

   Depositor

   Steven Vik

   Article

   Bae et al Biochim Biophys Acta. 2009 Sep;1787(9):1

   Insert

   atpBEFHAGDC
   

   Tags

   6-His beta subunit (atpD) and HA tag subunit a (a…

   Expression

   Bacterial

   Mutation

   All Cys codons changed to Ala.

   Available Since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAV-Camk2-NES-Twitch-NR

   Plasmid

   #173043

   Purpose

   TnC-based calcium sensor with green-red FRET pair ratiometric readout

   Depositor

   Yiyang Gong

   Article

   Zhang et al Commun Biol. 2021 Jul 29;4(1):924. doi:

   Insert

   NES-Twitch-NR
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CamK2

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. STAT3-GFP

   Plasmid

   #110495

   Purpose

   reporter of STAT3 signaling activity

   Depositor

   Michael Lewis

   Article

   Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

   Insert

   4 repeats of STAT3 binding site M67 at GFP promoter region
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLViP-LifeAct-iRFP670

   Plasmid

   #229699

   Purpose

   Lentiviral expression of LifeAct-iRFP670 in mammalian cells

   Depositor

   Alpha Yap

   Article

   Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

   Insert

   Synthetic construct LifeAct-mTurquoise gene
   

   Use

   Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3 myc CIITA (P#808)

   Plasmid

   #14650

   Depositor

   Matija Peterlin

   Article

   Kanazawa et al Immunity. 2000 Jan . 12(1):61-70.

   Insert

   CIITA (CIITA Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. p4xmts-mScarlet3_N1

   Plasmid

   #189774

   Purpose

   Construct for labelling mitochondria in mammalian cells using mitochondrial targeted mScarlet3

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   4xmts-mScarlet3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HA-TEAD1

   Plasmid

   #229411

   Purpose

   Lentiviral or overpexression of cDNA

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   TEAD1 (TEAD1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV5-Flag-XBP1s

    Plasmid

    #63680

    Purpose

    Mammalian expression of Flag tagged XBP1s

    Depositor

    Qingbo Xu, Lingfang Zeng

    Article

    Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

    Insert

    XBP1 (XBP1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    spliced

    Promoter

    CMV

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. Human Beta-catenin GFP

    Plasmid

    #71367

    Purpose

    mammalian expression of human Beta-catenin GFP

    Depositor

    Alpha Yap

    Article

    Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

    Insert

    Beta-catenin (CTNNB1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAcGP67A-mMD2-proA

    Plasmid

    #187878

    Purpose

    baculovirus protein expression of mouse MD-2 (residues 19–160) fused to a protein A tag

    Depositor

    Bruce Beutler

    Article

    Wang et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

    Insert

    MD2 (Ly96 Mouse)
    

    Expression

    Insect

    Mutation

    includes residues 19–160 of mouse MD-2

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. CARPID BASU-dCasRx

    Plasmid

    #153209

    Purpose

    express CARPID BASU-dCasRx fusion protein in mammalian cells

    Depositor

    Jian Yan, Liang Zhang

    Article

    Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

    Insert

    BASU-dCasRx
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    R239A/H244A/R858A/H863A in RfxCas13d

    Promoter

    EF-1a

    Available Since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF0799

    Plasmid

    #141705

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ATF3 (ATF3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CMV500 A-CREB

    Plasmid

    #33371

    Depositor

    Charles Vinson

    Article

    Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

    Insert

    CREB (Creb1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Dominant Negative (see notes)

    Promoter

    CMV

    Available Since

    Dec. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJZC43

    Plasmid

    #66565

    Purpose

    sgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 2XPP7
    PCP-VP64 IRES mCherry
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHInt-AppA-KanR-loxP

    Plasmid

    #232910

    Purpose

    Plasmid for marker free integration of E. coli appA at the phac1 locus of Cupriavidus necator.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

    Insert

    AppA (appA )
    

    Use

    Synthetic Biology

    Promoter

    Pj5

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Olig001

    Plasmid

    #170716

    Purpose

    Olig001 encodes a novel AAV capsid that exhibits preferential oligodendrocyte tropism in the CNS

    Depositor

    Thomas McCown

    Article

    Powell et al Gene Ther. 2016 Nov;23(11):807-814. doi:

    Insert

    Olig001 capsid gene / rep 2
    

    Use

    AAV

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. AbVec1.1-IGLC2-XhoI

    Plasmid

    #99575

    Purpose

    Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hCMV IE1 gene promoter

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLZ12Km2-P23R:TA:Xen5

    Plasmid

    #88902

    Purpose

    Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

    Depositor

    Thomas Proft

    Article

    Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

    Insert

    toxin-antitoxin + LuxABCDE
    

    Expression

    Bacterial

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

21. pcDNA3 Flag FKHR

    Plasmid

    #13507

    Depositor

    Kunliang Guan

    Article

    Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

    Insert

    FKHR (FOXO1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Dec. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLifeAct-mScarlet3_N1

    Plasmid

    #189767

    Purpose

    Construct for labelling actin filaments in mammalian cells with lifeact-mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    LifeAct-mScarlet3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTNT-B18R-6His

    Plasmid

    #58979

    Purpose

    expresses His-tagged B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Insert

    B18R
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7/Sp6

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDS-Red-N1 GABAA beta3

    Plasmid

    #109143

    Purpose

    The expression vector carries the red fluorescent protein tagged at the C-terminus of beta3 subunit of the GABAa receptor.

    Depositor

    Stefano Vicini

    Insert

    GABAa receptor
    

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    beta3 subunit of the GABAa receptor

    Promoter

    CMV

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti-CMV-MCS-GFP-SV-puro

    Plasmid

    #73582

    Purpose

    Third generation lentiviral vector expressing GFP and puromycin

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP tag

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p413 TEF NAPstar3

    Plasmid

    #232740

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar3 in S. cerevisiae.

    Depositor

    Bruce Morgan

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    NAPstar3
    

    Expression

    Yeast

    Available Since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMS59

    Plasmid

    #215680

    Purpose

    Cas9 + guide plasmid targeting ChrI split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    eef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1-AXL-V5/HIS

    Plasmid

    #200117

    Purpose

    Expression of human AXL receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human AXL receptor tyrosine kinase (AXL Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. HIV-1 integrase F185K/C280S in pET-15b

    Plasmid

    #61669

    Purpose

    HIV-1 integrase F185K/C280S in pET-15b

    Depositor

    Robert Craigie

    Article

    Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

    Insert

    HIV-1 integrase F185K/C280S
    

    Use

    Synthetic Biology

    Tags

    His

    Promoter

    T7 promotor

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pWay21-MKP-1 FL

    Plasmid

    #13469

    Depositor

    Anton Bennett

    Article

    Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

    Insert

    MKP-1 FL (Dusp1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

31. pTW2066

    Plasmid

    #236575

    Purpose

    ISYmu1 PDS3 gRNA2 TRV2 Architecture_B

    Depositor

    Steven Jacobsen

    Article

    Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

    Insert

    ISYmu1 TnpB
    

    Expression

    Plant

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLVX-CMV-Galpha-oA ONE-GO

    Plasmid

    #189732

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-oA ONE-GO
    GNAO (Gnao1 Rat)
    

    Use

    Lentiviral

    Mutation

    I250 (silent mutation in YFP)

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. MCS-TurboID MSCV-IRES-GFP

    Plasmid

    #207957

    Purpose

    Retroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the C-terminus

    Depositor

    Timothy Ley

    Article

    Day et al J Clin Invest. 2023 Dec 7:e176311. doi:

    Insert

    TurboID
    

    Use

    Retroviral; Turboid biotin proximity ligase

    Tags

    TurboID

    Expression

    Mammalian

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pPBpuro-miRFP670-HRasCT

    Plasmid

    #176030

    Purpose

    Encoding membrane-targeted miRFP670

    Depositor

    Michiyuki Matsuda

    Article

    Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

    Insert

    Membrane-targeted miRFP670
    

    Expression

    Mammalian

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. BAZ2AA-c002

    Plasmid

    #53623

    Purpose

    bacterial expression plasmid

    Depositor

    Nicola Burgess-Brown

    Insert

    BAZ2AA
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Promoter

    T7

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

36. SpyTag003-mKate2

    Plasmid

    #133452

    Purpose

    Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-mKate2
    

    Tags

    GSGSGS linker and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTRIP-GFP-IRF3

    Plasmid

    #127663

    Purpose

    Over-express EGFP-IRF3

    Depositor

    Nicolas Manel

    Article

    Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

    Insert

    IRF3 (IRF3 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pegRNA-GBA-10

    Plasmid

    #199271

    Purpose

    pegRNA used to induce GBA (c. 1226 A > G) mutation

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    GBA 1226AtoG pegRNA
    

    Expression

    Mammalian

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 6 x DBE-luc

    Plasmid

    #153160

    Purpose

    Luciferase reporter plasmid for FOXTF

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    luciferase
    

    Use

    Luciferase

    Promoter

    minP

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. DJ-1 pET15b

    Plasmid

    #60678

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

    Insert

    DJ-1 (PARK7 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

41. pGWB-nLUC

    Plasmid

    #174050

    Purpose

    Gateway compatible plasmid containing N-terminal luciferase for split-luciferase complementation assay

    Depositor

    Alberto Macho

    Article

    Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    N-terminal luciferase

    Expression

    Plant

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pLVUHshp53

    Plasmid

    #11653

    Purpose

    3rd generation lentiviral transfer vector expressing shp53 under an hUbiquitin promoter.

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    GFP, shRNA against TP53 (TP53 Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pBAD-HisD-CreiLOV

    Plasmid

    #192817

    Purpose

    Expresses CreiLOV in bacteria

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    CreiLOV
    

    Expression

    Bacterial

    Promoter

    araBAD promoter

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pRK5_mEGFP-YAP-MAMLD1

    Plasmid

    #237675

    Purpose

    For overexpression of mEGFP-YAP-MAMLD1

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    YAP-MAMLD1 (YAP1, MAMLD1 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pRK5_mEGFP-YAP-MAML2

    Plasmid

    #237674

    Purpose

    For overexpression of mEGFP-YAP-MAML2

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    YAP-MAML2 (YAP1, MAML2 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. PEmax_AAVS1_knockin_HDR_donor

    Plasmid

    #223000

    Purpose

    HDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PEmax
    

    Use

    Hdr donor

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST

    Plasmid

    #174007

    Purpose

    Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the control of an hSyn promoter. jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A.

    Depositor

    Mark Histed

    Article

    LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22

    Has Service

    AAV9

    Insert

    jGCaMP8s-P2A-ChrimsonR-ST
    

    Use

    AAV

    Tags

    6xHis and soma targeting motif

    Promoter

    hSyn

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pMal-Strep

    Plasmid

    #209345

    Purpose

    Protein expression vector with a TEV cleavable N-terminal 6xHis-MBP and C-terminal StrepTagII

    Depositor

    Lucia Strader

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pEVOL_PylRS(AF)-tRNA

    Plasmid

    #223511

    Purpose

    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

    Depositor

    Edward Lemke

    Article

    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

    Inserts

    Pyrrolysyl-tRNA Synthetase (AF) (2 copies) (pylS Methanosarcina mazei)
    tRNA Pyl
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y306A; Y384F

    Promoter

    araBAD, rrnB, and glnS and proK

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. Active Wnt5A-V5

    Plasmid

    #43813

    Depositor

    Xi He

    Article

    MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

    Insert

    WNT5A (WNT5A Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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