175,581 results

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pcDNA3.1his p66shc (corrected)

Plasmid

#32574

Depositor

Toren Finkel

Article

Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

Insert

p66shc (SHC1 Human)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pLv-HSA-uDys/eGFP

Plasmid

#26810

Depositor

Jeffrey Chamberlain

Article

Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

Insert

dystrophin (DMD Human)

Use

Lentiviral; Lentiviral plasmid backbone from l. n…

Tags

eGFP

Mutation

deleted R4-R23 deleted exons 71-78

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pPaxillin-PSmOrange

Plasmid

#31923

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Tags

Paxillin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV4-NIK-HA

Plasmid

#27554

Depositor

Shao-Cong Sun

Article

Xiao et al J Biol Chem. 2000 Jul 14. 275(28):21081-

Insert

NIK (MAP3K14 Human)

Tags

HA

Expression

Mammalian

Mutation

NIK starts at amino acid 3; C52R and R139H mutati…

Promoter

CMV

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-FLEX-ArchT-GFP

Plasmid

#28307

Purpose

Cre-dependent viral expression of ArchT-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual LIC cloning vector (5A)

Plasmid

#30121

Depositor

Scott Gradia

Type

Empty backbone

Expression

Insect

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pET Biotin His6 GFP LIC cloning vector (H6-msfGFP)

Plasmid

#29725

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, AviTag (biotin), SiriusGFP, and TEV cleava…

Expression

Bacterial

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2/v5-DEST hGlut2

Plasmid

#18086

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

Glut2 (SLC2A2 Human)

Expression

Mammalian

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-TREX1(D18N)

Plasmid

#27220

Depositor

Judy Lieberman

Article

Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

Insert

TREX1 (TREX1 Human)

Tags

GFP

Expression

Mammalian

Mutation

D18N sequence 52-GAC changed to AAC, resulting i…

Available Since

Jan. 26, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pLew100::NLS-ISceI-HA

Plasmid

#21299

Depositor

Nina Papavasiliou

Article

Boothroyd et al Nature. 2009 May 14;459(7244):278-81. do

Insert

I-SceI (SCEI )

Tags

HA and NLS

Expression

Mammalian

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-FLEX-GT

Plasmid

#26198

Depositor

Edward Callaway

Article

Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Enhanced green fluorescent protein, TVA (eGFP TVA is a mouse codon optimized version of an avian gene., Mouse)

Use

AAV and Cre/Lox

Mutation

TVA mammalian codon optimized

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pLNCX2-Scrib

Plasmid

#24600

Depositor

Senthil Muthuswamy

Article

Zhan et al Cell. 2008 Nov 28. 135(5):865-78.

Insert

Scribble (SCRIB Human)

Expression

Mammalian

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-RhoB

Plasmid

#23225

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

RhoB (Rhob Rat)

Tags

GFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-K27 histone methylation reporter

Plasmid

#22865

Depositor

Alice Ting

Article

Lin et al J Am Chem Soc. 2004 May 19. 126(19):5982

Insert

K27 HMR

Tags

CFP and YFP

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-K9 histone methylation reporter

Plasmid

#22866

Depositor

Alice Ting

Article

Lin et al J Am Chem Soc. 2004 May 19. 126(19):5982

Insert

K9 HMR

Tags

CFP and YFP

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pFNF

Plasmid

#22687

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Neo*

Use

Mouse Targeting

Expression

Bacterial and Mammalian

Mutation

FRT-pPGK-pEM7-Neo*-bpA-FRT

Available Since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP43

Plasmid

#22583

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP43 (USP43 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

not FL, missing first 108 aa

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-HA-USP29

Plasmid

#22599

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP29 (USP29 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
mRFP-human APPL1

Plasmid

#22202

Depositor

Pietro De Camilli

Article

Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

Insert

Adaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)

Tags

mRFP

Expression

Bacterial and Mammalian

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pmRFP_a_tubulin_C1

Plasmid

#21040

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Tags

pmRFP

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-NL1AB

Plasmid

#15262

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Neuroligin 1 with inserts A and B (Nlgn1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Jan. 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pAd 2B Ngn3-I-nGFP

Plasmid

#19410

Depositor

Douglas Melton

Article

Zhou et al Nature. 2008 Oct 2;455(7213):627-32. doi

Insert

neurogenin 3 (Neurog3 Mouse)

Use

Adenoviral and Gateway Cloning

Tags

GFP and IRES

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
HA-EglN3-pcDNA3

Plasmid

#18960

Depositor

William Kaelin

Article

Lee et al Cancer Cell. 2005 Aug . 8(2):155-67.

Insert

Egg laying Nine - 3 (EGLN3 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C3-hYAP2

Plasmid

#17844

Depositor

Marius Sudol

Article

Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

Insert

YAP (YAP1 Human)

Tags

EGFP

Expression

Bacterial and Mammalian

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-SENP8

Plasmid

#18066

Depositor

Edward Yeh

Article

Gao et al Nat Cell Biol. 2006 Oct . 8(10):1171-7.

Insert

SENP8 (SENP8 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pCEP4 Smad4

Plasmid

#16483

Depositor

Bert Vogelstein

Article

Riggins et al Nat Genet. 1996 Jul . 13(3):347-9.

Insert

Smad4 (SMAD4 Human)

Tags

Double HA

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
VL3 (Flag-Mad2)

Plasmid

#16047

Depositor

Robert Benezra

Article

Wassmann et al EMBO J. 2003 Feb 17. 22(4):797-806.

Insert

Mad2 (MAD2L1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCX-EGFP c-Abl

Plasmid

#15007

Depositor

Raymond Birge

Insert

c-Abl (Abl1 Mouse)

Tags

IRES EGFP

Expression

Mammalian

Available Since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only
pGFP-E p27 (CC#631)

Plasmid

#15192

Depositor

Connie Cepko

Article

Dyer et al J Neurosci. 2001 Jun 15. 21(12):4259-71.

Insert

p27 (Cdkn1b Mouse)

Use

Retroviral

Tags

Flag and His

Expression

Mammalian

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5 Smad1-HA

Plasmid

#14956

Depositor

Joan Massague

Article

Liu et al Nature. 1996 Jun 13. 381(6583):620-3.

Insert

Smad1 (SMAD1 Human)

Tags

HA

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL-FLKif3A

Plasmid

#13742

Depositor

Linda Wordeman

Article

Ginkel et al Mol Biol Cell. 2000 Dec . 11(12):4143-58

Insert

mouse Kif3A (Kif3a Mouse)

Use

Cre/Lox

Expression

Mammalian

Available Since

Jan. 29, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pLI50

Plasmid

#13573

Depositor

Chia Lee

Article

Lee et al Gene. 1991 Jul 15. 103(1):101-5.

Type

Empty backbone

Use

S. aureus-e. coli shuttle vector

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 MEKK1 KD

Plasmid

#12180

Depositor

Gary Johnson

Article

Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

Insert

MEKK1 (Map3k1 Mouse)

Tags

His and Xpress

Expression

Mammalian

Mutation

Kinase dead: K1253M; V1467L

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
pCerulean-VSVG

Plasmid

#11913

Depositor

Jennifer Lippincott-Schwartz

Article

Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

Insert

VSVG

Expression

Mammalian

Mutation

F64L, S65T, Y66W, S72A, N146I, Y145A, H148D, M15…

Available Since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe H-Ras 12V

Plasmid

#12545

Depositor

Channing Der

Article

Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

Insert

H-Ras 12V (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

12V constituitively activated.

Available Since

Sept. 8, 2006

Availability

Academic Institutions and Nonprofits only
LL - hOCT4i -2

Plasmid

#12197

Depositor

George Daley

Article

Zaehres et al Stem Cells. 2005 Mar . 23(3):299-305.

Insert

OCT4 - RNAi (POU5F1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Sept. 6, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCL-MFG-BRCA1

Plasmid

#12341

Depositor

Inder Verma

Article

Ruffner et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

Insert

BRCA1 (BRCA1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pL3-TRE-LucGFP-2L

Plasmid

#11685

Depositor

Geoff Wahl

Article

Wong et al Nucleic Acids Res. 2005 . 33(17):e147.

Insert

Luciferase-EGFP

Use

Cre/Lox

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCINeo-myc-Itch (C822S)/2860

Plasmid

#11428

Depositor

Allan Weissman

Article

Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

Insert

Itch (Itch Mouse)

Tags

myc

Expression

Mammalian

Mutation

cysteine at 822 mutated to serine

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
1435 pSG5L Flag HA

Plasmid

#10791

Purpose

Mammalian expression empty backbone.

Depositor

William Sellers

Type

Empty backbone

Tags

Flag and HA

Expression

Mammalian

Available Since

Sept. 28, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-zeo smallT

Plasmid

#8583

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen (SV40gp7 simian virus)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only
COM-epegRNA-Dnmt1

Plasmid

#211376

Purpose

Dnmt1 targeting epegRNA with COM insertion in gRNA scaffold for v3b PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

COM-epegRNA

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP

Plasmid

#188778

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Hs.cargo(Cre)

Plasmid

#174863

Purpose

Expresses human Peg10 cargoRNA encoding Cre recombinase

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

Cre flanked by HsPeg10 UTRs

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-Cas13X.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

Plasmid

#171379

Purpose

Expression vector for encoding a human codon-optimized Cas13X.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

Depositor

Hui Yang

Article

Xu et al Nat Methods. 2021 May;18(5):499-506. doi

Insert

humanized Cas13X.1

Expression

Mammalian

Promoter

CMV, U6

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pIR-CMV-SCN2A-Variant-1-IRES-mScarlet

Plasmid

#162279

Purpose

Eukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

Depositor

Al George

Article

DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

Insert

SCN2A Variant 1, stabilized (SCN2A Human)

Tags

IRES-mScarlet

Expression

Mammalian

Mutation

IVS intron inserted after bp4551.

Promoter

CMV

Available Since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-HyPer7-MEM

Plasmid

#136465

Purpose

Mammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Farnselation tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 SARS-CoV-2 ORF8

Plasmid

#141278

Purpose

Gateway-compatible Entry vector, with insert of ORF8 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF8 (ORF8 SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

Plasmid

#139987

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Mito-mEosEM

Plasmid

#132706

Purpose

application in Correlative light-electron microscopy

Depositor

Pingyong Xu

Article

Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

mitochondria targeting sequence

Tags

mEosEM

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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175,581 results