4,133 results

1. pAV5S-F30-2xdBroccoli

   Plasmid

   #66845

   Purpose

   Expresses 5S rRNA fused to two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

   Depositor

   Samie Jaffrey

   Article

   Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

   Insert

   Two dimeric Broccoli aptamers in the F30 scaffold
   

   Use

   Tags

   5S rRNA

   Expression

   Mammalian

   Mutation

   Promoter

   5S

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRK5-myc-RhoA-T19N

   Plasmid

   #12963

   Depositor

   Gary Bokoch

   Insert

   RhoA DN (RHOA Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   T19N Dominant negative

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBiFC-CrN173

   Plasmid

   #22016

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

   Insert

   CrN173
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSV Sport SREBP-1c

   Plasmid

   #8883

   Depositor

   Bruce Spiegelman

   Article

   Tontonoz et al Mol Cell Biol. 1993 Aug . 13(8):4753-9.

   Insert

   SREBP-1c (Srebf1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Active version of SREBP-1c.

   Promoter

   Available since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro

   Plasmid

   #141387

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-orf6 (ORF6 SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available since

   April 9, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pDusk

   Plasmid

   #43795

   Purpose

   Empty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.

   Depositor

   Andreas Moeglich

   Article

   Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pmTurquoise2-N1

   Plasmid

   #60561

   Purpose

   Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR11 CA NFAT1

   Plasmid

   #11792

   Depositor

   Anjana Rao

   Insert

   NFAT1 (Nfatc2 Mouse)
   

   Use

   Tags

   HA

   Expression

   Bacterial

   Mutation

   Constitutively active: multiple serine to alanine…

   Promoter

   Available since

   Aug. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQUAST

   Plasmid

   #24349

   Depositor

   Liqun Luo

   Article

   Potter et al Cell. 2010 Apr 30. 141(3):536-48.

   Insert

   5xQUAS-Pmin promoter
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 12, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3 Hygro HA Akt2

    Plasmid

    #16000

    Depositor

    Morris Birnbaum

    Insert

    Akt2 (AKT2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIRESneo-PA-GFP-alpha Tubulin

    Plasmid

    #12297

    Depositor

    Patricia Wadsworth

    Article

    Tulu et al Curr Biol. 2003 Oct 28. 13(21):1894-9.

    Insert

    alpha Tubulin (TUBA1B Human)
    

    Use

    Tags

    PA-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pECFP-DEVDR-Venus

    Plasmid

    #24537

    Depositor

    Peter Sorger

    Article

    Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

    Insert

    ECFP-DEVDR-Venus
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

13. mOrange2-N1

    Plasmid

    #54568

    Purpose

    Localization: N1 Cloning Vector, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson, Roger Tsien, Nathan Shaner

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLVX-EF1alpha-SARS-CoV-2-nsp4-2xStrep-IRES-Puro

    Plasmid

    #141369

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp4 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pcDNA3.1/opto-a1AR-EYFP

    Plasmid

    #20947

    Purpose

    Mammalian expression of opto-a1AR fused to EYFP for optical control of GPCR signaling

    Depositor

    Karl Deisseroth

    Article

    Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

    Insert

    opto-a1AR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHJ421

    Plasmid

    #20680

    Depositor

    Stephen Shaw

    Article

    Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

    Insert

    Ezrin (EZR Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLVX-EF1alpha-SARS-CoV-2-orf9b-2xStrep-IRES-Puro

    Plasmid

    #141392

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-orf9b
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pEGFP-N1-ACTR3

    Plasmid

    #8462

    Depositor

    Matthew Welch

    Article

    Welch et al J Cell Biol 1997 Jul 28;138(2):375-84.

    Insert

    ACTR3 (ACTR3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p181 pK7-HDAC1 (GFP)

    Plasmid

    #11054

    Depositor

    Ramesh Shivdasani

    Article

    Tou et al Mol Cell Biol. 2004 Apr . 24(8):3132-9.

    Insert

    HDAC1 (Hdac1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK7-HA-S6K1-KR

    Plasmid

    #8985

    Depositor

    John Blenis

    Article

    Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K100R, kinase-inactive

    Promoter

    Available since

    Jan. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDONR223 SARS-CoV-2 S

    Plasmid

    #149329

    Purpose

    Gateway-compatible Entry vector, with insert of the spike (S) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    S (S SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. mTLR4 flag

    Plasmid

    #13087

    Depositor

    Ruslan Medzhitov

    Insert

    TLR4 (Tlr4 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Amino acids 1-19 have been replaced with mammalia…

    Promoter

    Available since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 1066 pBabe puroL PTEN

    Plasmid

    #10785

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCFJ350 - MCS(ttTi5605, II)

    Plasmid

    #34866

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

    Insert

    ttTi5605 targeting
    

    Use

    Worm targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pQLinkH

    Plasmid

    #13667

    Depositor

    Konrad Buessow

    Article

    Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

    Insert

    multi cloning site
    

    Use

    Co-expression

    Tags

    His, TEV, and attB1

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mTOR_1 shRNA

    Plasmid

    #1855

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    mTOR (MTOR Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. paavCAG-pre-mGRASP-mCerulean-2A-nls-mCherry

    Plasmid

    #34911

    Depositor

    Jinhyun Kim

    Article

    Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

    Insert

    presynaptic mGRASP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Nov. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDD286 (TagRFP^SEC^3xMyc)

    Plasmid

    #70684

    Purpose

    TagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.

    Depositor

    Bob Goldstein

    Insert

    TagRFP-T-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xMyc and C. elegans codon-optimized TagRFP-T

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mApple-VE-Cadherin-N-10

    Plasmid

    #54959

    Purpose

    Localization: Tight Junctions, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    VE-Cadherin (CDH5 Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    I503T

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBabe-Puro-MEK-DD

    Plasmid

    #15268

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    Map2k1 (Mapk1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    S218D S222D

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge