20,116 results

Showing: 3631 - 3660 of 20116 results

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pCMV-Sport6-CD63-pHluorin

Plasmid

#130901

Purpose

Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD63-pHluorin (CD63 Aequorea victoria, Human)

Tags

pHluorin

Expression

Mammalian

Mutation

pHluorin inserted between Gln-36 and Leu-37 in ex…

Promoter

CMV

Available Since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pBAC-lacZ

Plasmid

#13422

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Cre/Lox

Expression

Bacterial

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pFLP-hyg

Plasmid

#87831

Purpose

Expresses FLP recombinase with hyg selection marker

Depositor

Pep Charusanti

Article

Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

Insert

hyg

Tags

None

Expression

Bacterial

Mutation

None

Promoter

heat-shock inducible

Available Since

Aug. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_hTRIM32

Plasmid

#69541

Purpose

Expresses human TRIM32 fused to c-terminal EGFP

Depositor

Martin Dorf

Article

Fu et al PLoS Pathog. 2015 Jun 9;11(6):e1004960.

Insert

tripartite motif containing 32 (TRIM32 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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BPK2660

Plasmid

#70709

Purpose

Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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LXRalpha_pcDNA3.1

Plasmid

#177621

Purpose

Full-length human LXR alpha cDNA sequence in pcDNA3.1+

Depositor

Thomas Burris

Article

Griffett et al ACS Chem Biol. 2022 Apr 13. doi: 10.1021

Insert

LXR alpha (NR1H3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP30

Plasmid

#22578

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP30 (USP30 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_ATP1.0

Plasmid

#167577

Purpose

Express the ATP sensor GRAB_ATP1.0 in neurons

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP sensor GRAB_ATP1.0 (P2RY1 Human)

Use

AAV

Promoter

hSyn

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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Nuclear cpGFP

Plasmid

#215707

Purpose

Expresses cpGFP in the nucleus

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

cpGFP

Use

Lentiviral

Tags

FLAG, HA, and NLS

Expression

Mammalian

Mutation

circularly permuted GFP

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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OKSIM

Plasmid

#24603

Depositor

Jose Cibelli

Article

Ross et al Stem Cells Dev. 2010 Aug;19(8):1221-9

Insert

Oct4 KLF4 Sox2 c-Myc (SOX2, POU5F1, MYC, KLF4 Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

Oct4-2A-KLF4-2A-Sox2-IRES-Myc

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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PB_iASCL1_P2A_DLX2_T2A_mCherry_Puro

Plasmid

#168806

Purpose

PiggyBac transposon construct with doxycycline inducible ASCL1, DLX2 + mCherry expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

ASCL1-P2A-DLX2-T2A-mCherry

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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AviTag-SpyCatcher

Plasmid

#72326

Purpose

Bacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

AviTag-SpyCatcher

Tags

N-term His6 tag and TEV cleavage site

Expression

Bacterial

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-SOX9

Plasmid

#141404

Purpose

Inducible Expression of SOX9

Depositor

Lorenz Studer

Article

Tchieu et al Nat Biotechnol. 2019 Mar;37(3):267-275.

Insert

SOX9 (SOX9 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_MeCP2(WT)

Plasmid

#110186

Purpose

Expression of mouse MeCP2 (WT) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 FL (mouse cDNA) (Mecp2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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mKO2-N1

Plasmid

#54625

Purpose

Localization: N1 Cloning Vector, Excitation: 551, Emission: 565

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Sakaue-Sawano et al Cell. 2008 Feb 8;132(3):487-98. doi: 10.

Type

Empty backbone

Tags

mKO2

Expression

Mammalian

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-Luciferase-IRES-Blast-WPRE

Plasmid

#108542

Purpose

Lentivirus vector expressing Luciferase with blasticidin resistance

Depositor

Javier Alcudia

Inserts

Luciferase
Neomycin

Use

Lentiviral

Promoter

EF1apha and PGK

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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scAAV-sgRNA-GFP

Plasmid

#177935

Purpose

Expresses sgRNA and can be packaged into AAV particles for somatic delivery into Cas9 transgenic mice

Depositor

Roland Rad

Article

Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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ADORA3-Tango

Plasmid

#66212

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

ADORA3 (ADORA3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-VSVM

Plasmid

#64086

Purpose

Expression vector for Vesicular Stomatitis Virus Indiana strain matrix gene

Depositor

Ian Wickersham

Insert

VSV matrix protein

Expression

Mammalian

Promoter

CAG

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pJC175e

Plasmid

#79219

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PPSP gIII, luxAB

Expression

Bacterial

Promoter

PPSP

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV2-SEP-GluA1 (M1)

Plasmid

#64942

Purpose

mammalian expression of rat GluA1

Depositor

Jeremy Henley

Article

Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

Insert

GluA1 (Gria1 Rat)

Tags

SEP (superecliptic pHluorin)

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-SEpHluorin

Plasmid

#32001

Depositor

Sergio Grinstein

Article

Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

Insert

mCherry

Tags

Superecliptic pHluorin

Expression

Mammalian

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pDG02583

Plasmid

#104129

Purpose

Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

NEDD8-specific protease 1 (NEDP1)

Tags

14x Histidine tag, Maltose binding protein, and S…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV6M-Pak1

Plasmid

#12209

Depositor

Jonathan Chernoff

Article

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pVT100U-mtGFP

Plasmid

#45054

Depositor

Benedikt Westermann

Article

Westermann et al Yeast. 2000 Nov;16(15):1421-7.

Insert

mtGFP

Expression

Yeast

Promoter

ADH

Available Since

May 17, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-mCherry-IRES-Flpo

Plasmid

#55634

Purpose

Expresses Flpo in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde and AAV1

Inserts

mCherry
Flpo

Use

AAV

Expression

Mammalian

Promoter

Ef1a and Ef1a/IRES

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pFH2.2_mCherry-MS2x12

Plasmid

#205521

Purpose

Express mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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mNeonGreen

Plasmid

#189976

Purpose

Part 3-4-5 plasmid to introduce mNeonGreen into circRNAs

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Use

Cloning

Promoter

N/A

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-Bcl2

Plasmid

#18003

Depositor

Clark Distelhorst

Article

Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

Insert

Bcl-2 (BCL2 Human)

Tags

Flag

Expression

Mammalian

Available Since

June 4, 2008

Availability

Academic Institutions and Nonprofits only

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psfTn5

Plasmid

#79107

Purpose

bacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutations

Depositor

Sten Linnarsson

Insert

transposase 5

Tags

His

Expression

Bacterial

Mutation

E54K and L372P

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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20,116 results