We narrowed to 15,043 results for: NOL
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Plasmid#140465PurposeCRISPR/Cas9 engineering with nourseothricin selectionDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only
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KG#470
Plasmid#110930PurposeExpresses proteins pan-neuronally in C. elegans (entire nervous system)DepositorInsertsrab-3 promoter
unc-54 3' control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PRKCA
Plasmid#67115PurposeStandard for cell-free expression benchmarking.DepositorInsertPRKCA Protein kinase C alpha type (PRKCA Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-HD118
Plasmid#172655PurposeExpressing paired pegRNAs from human U6 and H1 promoters to make 118-bp deletion on HPRT1 geneDepositorInsertpegRNA-HD118A/pegRNA-HD118B
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3_4xUAS_CP-candidate_luc+
Plasmid#125154PurposeSTAP-seq luciferase validation vector, which harbors an 4x UAS array upstream of the CP position (candidates are inserted at CP position) for the recruitement of GAL4-DBD tagged proteinsDepositorInsert4 x upstream activating sequence (UAS)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA
Plasmid#87560PurposeExpresses aa397-423 of Flp recombinase fused to PYL CIDDepositorInsertFlpO aa397-423
UseCre/Lox and Synthetic BiologyTagsPYLExpressionMammalianMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA
Plasmid#87559PurposeExpresses aa1-396 of Flp recombinase fused to ABI CIDDepositorInsertFlpO aa1-396
UseCre/Lox and Synthetic BiologyTagsABIExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLP762_L2pV1_OR-OCS_TCTP-Flp_s35S-Cre
Plasmid#192421PurposeTo act as the 2-input state of the OR gate.DepositorInsertAct2::FRT-loxP-OCS-FRT-loxP::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
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EK0499 SFFV-mCherry-SGc(PPIB.s1)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191183PurposeExpression of PPIB sensor in mammalian cellsDepositorInsertmCherry:PPIB.s1:EGFP-3xMS2
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBM13-sd4U
Plasmid#179691PurposeBacterial expression of base editorDepositorInsert[sd4U] UGI-TALE3-DddA-G1397-N (Q1310R + S1330I + T1380I)
UseTagsExpressionBacterialMutationQ1310R, S1330I, T1380IPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-Actin-7
Plasmid#55462PurposeLocalization: Actin, Excitation: 462, Emission: 492DepositorInsertActin (ACTB Human)
UseTagsmTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
EK0289 SFFV-mCherry-SG(s17d9b)SG-EGFP (FLP-IN)
Plasmid#191142PurposeExpression of Sensor 2 (for EK0211) in mammalian cells; mCherry marker, EGFP output.DepositorInsertmCherry:s17d9b:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 WT
Plasmid#66139PurposeExpresses the wild type of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pCfB6679
Plasmid#106158PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6638, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pJBL729
Plasmid#140399PurposeExpresses CadC-regulated 3WJdB RNA aptamer driven by T7 promoter with the cadO sequence 5BP downstream from the promoterDepositorInsertT7-cadO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pPV230.13
Plasmid#14902DepositorInsertStrongyloides stercoralis transitional endoplasmic reticulum ATPase promoter region
UseTagsExpressionWormMutationSs era-1 promoter region as a HindIII-PstI fragme…PromoterAvailable sinceJuly 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pJBL718
Plasmid#140388PurposeExpresses MobR-regulated 3WJdB RNA aptamer driven by T7 promoter with the mobO sequence 5BP downstream from the promoterDepositorInsertT7-mobO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish-gRNA-0007
Plasmid#42247PurposegRNA targeted to zebrafish gene th1DepositorInsertgRNA-th1 (th Zebrafish)
UseCRISPR; Zebrafish expressionTagsExpressionMutationPromoterT7Available sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PPARG
Plasmid#67099PurposeStandard for cell-free expression benchmarking.DepositorInsertPPARG (PPARG Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Skp-hLYZ
Plasmid#83924PurposeCo-expresses cytoplasmic copies of the Skp protein folding chaperone and wild type human lysozyme..DepositorInsertsUseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-DEST-R1R2-pNeo
Plasmid#141057PurposeMutisite Gateway mediated vector constructionDepositorInsertNeomycin resistance gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPTXLUC
Plasmid#68504Purposecyclic nucleotide reporter for bacteria or plant cellsDepositorInsertOPTX promoter (AT1G33440 Mustard Weed)
UseTagsluciferaseExpressionBacterial and PlantMutationPromoterOPTXAvailable sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCoofy62
Plasmid#122014PurposeMammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His6-Smt3Star (SumoStar*) and different C-tag optionsDepositorTypeEmpty backboneUseTagsHis6-Smt3Star and Multi3: depending on the LP2 pr…ExpressionMammalianMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCfB6681
Plasmid#106157PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6637, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PSMC1
Plasmid#67087PurposeStandard for cell-free expression benchmarking.DepositorInsertPSMC1 (PSMC1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationE275G, I287V and R294GPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pENTR-L1-PGK-Neo-L2
Plasmid#141007PurposeMutisite Gateway mediated vector constructionDepositorInsertNeomycin resistance gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEU3-NII-HLICNot
Plasmid#140181PurposeUsed for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, 6x His-tag and NotI restriction siteDepositorTypeEmpty backboneUseExpression vector for a cell-free systemTagspEU3-NII-HLICNotExpressionMutationPromoterT7Available sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-mdlC
Plasmid#134590PurposeLow Phosphate inducible mdlC benzylformate decarboxylaseDepositorInsertmdlC (benzylformate decarboxylase) C -terminal his tag
UseTags6x-HisExpressionBacterialMutationPromoteryibDpAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-KA
Plasmid#64981PurposeExpresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E105K, E108APromoterAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMB1-spect-PthrC3-eGFP
Plasmid#107409PurposeThis plasmid contain promoter PthrC3, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterPthrC3Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXylA-agrCA-IV
Plasmid#53438Purposexylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
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