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171,784 results

Showing: 6151 - 6200 of 171784 results

  3. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid
    #213026
    Purpose
    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213026-plasmid-map-sequence-id-422069

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  14. pGL3-NFAT luciferase

    Plasmid
    #17870
    Purpose
    3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue
    Depositor
    Jerry Crabtree
    Article
    Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.
    Insert
    3x NFAT binding sequence (IL2 )
    Use
    Luciferase
    Tags
    IL2 promoter and Luciferase
    Expression
    Mammalian
    Promoter
    IL-2 promoter
    Available Since
    April 29, 2008
    Availability
    Academic Institutions and Nonprofits only
    17870-plasmid-map-sequence-id-325133

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  16. pSR657

    Plasmid
    #65214
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Article
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-2 cap
    Use
    AAV
    Expression
    Insect
    Promoter
    p10 and polyhedrin
    Available Since
    June 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    65214-plasmid-map-sequence-id-116341

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  24. mCherry-7aa-cb5-pEGFP-C1

    Plasmid
    #177407
    Purpose
    To express mCherry fused to endoplasmic reticulum (ER) targeting sequence "Cb5". mCherry faces the cytosol.
    Depositor
    David Andrews
    Insert
    Cb5 tail anchor (Cyb5r4 Rat)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177407-plasmid-map-sequence-id-348154

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  25. LSB-hsa-miR-192-3p

    Plasmid
    #103303
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-192-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-192-3p target (MIR192 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103303-plasmid-map-sequence-id-195655

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  26. LSB-hsa-miR-192-5p

    Plasmid
    #103304
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-192-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-192-5p target (MIR192 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103304-plasmid-map-sequence-id-195657

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  32. hFerritinH

    Plasmid
    #122652
    Purpose
    Expression of hFerritinH in bacterial cells
    Depositor
    Trevor Douglas
    Insert
    ferritin heavy chain 1 (FTH1 Human)
    Expression
    Bacterial
    Available Since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    122652-plasmid-map-sequence-id-237549

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  33. pAAV.hSyn.iGluSnFR3.v857.PDGFR

    Plasmid
    #178329
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Has Service
    AAV1
    Insert
    pAAV hSyn iGluSnFR3 v857.PDGFR
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    IgK-chain, Myc epi tag, and PDGFR TM Domain
    Expression
    Mammalian
    Available Since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178329-plasmid-map-sequence-id-361540

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  34. AbVec2.0-mIghg1

    Plasmid
    #127158
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG1 isotype
    Depositor
    Hedda Wardemann
    Insert
    Ighg1 (Ighg1 Mouse)
    Expression
    Mammalian
    Promoter
    hCMV IE1 gene promoter
    Available Since
    Nov. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    127158-plasmid-map-sequence-id-256842

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  48. TRUPATH Triple GaoA

    Plasmid
    #196051
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoA-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196051-plasmid-map-sequence-id-385865

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  50. pLenti6M SGLT1

    Plasmid
    #194146
    Purpose
    Expresses SGLT1 protein in mammalian cells
    Depositor
    Jun Liu
    Insert
    SLC5A1 (SLC5A1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194146-plasmid-map-sequence-id-380701

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Showing: 6151 - 6200 of 171784 results