We narrowed to 40,591 results for: tro
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Plasmid#239799PurposeExpress mEGFP tagged mouse CENPT chimeric protein containing the rat CENPT histone fold domainDepositorInsertMmCENPT--Rn-Histone Fold Domain
UseRetroviralTagsmEGFPExpressionMammalianMutationPromoterMoloney Murine Leukemia Virus (MMLV) LTR promoterAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-Trim21-OLLAS
Plasmid#237438PurposeExpresses mouse Trim21 ubiquitin ligase tagged with OLLAS at C-term; made for in vitro transcription (T7 promoter)DepositorInsertTrim21 (Trim21 Mouse)
UseTagsOLLASExpressionMammalianMutationPromoterT7Available sinceJune 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-MmCENP-W
Plasmid#237433PurposeExpresses Mus musculus CENP-W; untagged; made for in vitro transcriptionDepositorInsertMus musculus CENP-W (Cenpw Mouse)
UseTagsExpressionMammalianMutationPromoterT7Available sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-MmCENPT-GFP
Plasmid#237426PurposeExpresses Mus musculus CENP-T tagged with GFP at C-term; made for in vitro transcription (T7 promoter)DepositorInsertMus musculus CENP-T (Cenpt Mouse)
UseTagsGFPExpressionMammalianMutationPromoterT7Available sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUGW U6 gLacZ dCas9-KRAB-T2a-GFP
Plasmid#234883Purposenon-targeting CRISPRi controlDepositorInsertL1HS gRNA
UseCRISPR and LentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCARGO-RMCE-Airn
Plasmid#233266PurposeCARGO plasmid containing ~90kb dox-inducible Airn transgeneDepositorInsertAirn (Airn Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT478
Plasmid#228286PurposeConstitutive mutant rPhlTA expression with strong promoterDepositorInsertrphlTA mutant
UseSynthetic BiologyTagsThree copies of VP16 (VP48)-Nuclear localization …ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143KPromoterKpGAPDH promoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Hyg-ADE2
Plasmid#232104PurposeExpresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRTagsExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterGAL7Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Kan-ADE2
Plasmid#232105PurposeExpresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRTagsExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterGAL7Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS-miRFP670-TRF1
Plasmid#227378PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, WT (TERF1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
iLID-miRFP670-TRF1
Plasmid#227385PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric iLID (TERF1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-NS3/4A S139A
Plasmid#186885PurposeDoxycycline-dependent expression of catalytically inactive NS3/4A protease gene (S140A) in mammalian cells by retroviral transductionDepositorInsertHCV NS3/4A protease with S139A mutation
UseRetroviralTagsExpressionMutationS139APromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-WHG cGAS-HA
Plasmid#186867PurposeDoxycycline-dependent expression of white-handed gibbon cGAS gene in mammalian cells by retroviral transductionDepositorInsertWhite-handed gibbon cGAS with C-terminal HA tag
UseRetroviralTagsHAExpressionMutationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-IVT_ACC-msfGFP[r5M]_ACC-mEBFP2
Plasmid#221082PurposePlasmid for producing DNA Templates for in vitro transcription. Produces msfGFP[r5M] and mEBFP2 from IVT mRNA.DepositorInsertACC-msfGFP[r5M]_ACC-mEBFP2
UseSynthetic BiologyTagsExpressionMutationPromoterCMV, T7Available sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_+15.2prox1a:EGFP_XCA:DsRed2
Plasmid#218206PurposeZebrafish +15.2prox1a enhancer reporter in the FCLV from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert+15.2prox1a
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHis-WzzE
Plasmid#196661PurposeExpression of N-terminally His6-tagged WzzE from Pectobacterium atrosepticum in E.coliDepositorInsertWzzE
UseTagsTEV-cleavable His6ExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGST-WzzE
Plasmid#196662PurposeExpression of N-terminally GST-tagged WzzE from Pectobacterium atrosepticum in E.coliDepositorInsertWzzE
UseTagsTEV-cleavable GSTExpressionBacterialMutationValine insertion at position 2.PromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHis-mCherry-WzzE
Plasmid#196663PurposeExpression of N-terminally His6-mCherry-tagged WzzE from Pectobacterium atrosepticum in E.coliDepositorInsertWzzE
UseTagsHis and mCherryExpressionBacterialMutationValine insertion at position 2 of WzzE.PromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-GST-distrepII
Plasmid#196666PurposeExpression of C-terminally GST-distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
UseTagsGST and TEV-cleavable di-strep tag IIExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-strep-mClover3
Plasmid#196668PurposeExpression of C-terminally distrep tag II-mClover3-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
UseTagsTEV-cleavable di-strep tag II and mClover3ExpressionBacterialMutationPromoteraraBADAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4QE2Crimson
Plasmid#218280PurposeIntroduction of a bicistronic expression cassette of yEGFP and E2Crimson under the control of the SkGAL2 promoterDepositorInsertpURA3>KlURA3>tKlURA3-pSkGAL2>yEGFP>ERBV1.2A>E2Crimson>tURA3
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-2xflox-BRX-eGFP-NLS
Plasmid#217535PurposeAAV vector to drive the expression of eGFP in genetically defined cell populations under the control of the 4xBRE regulatory element of SMAD1 transcription factor and miniXon splicing casette in vivoDepositorArticleInsert4X BRE reporter and miniXon casette (Smad1 Mouse)
UseAAVTagsExpressionMammalianMutationPromoterBMP response element (BRE)Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF702
Plasmid#209650PurposeSuicide plasmid carrying a neomycin resistance marker flanked by homologous regions of the Mth60-fimbria encoding operon of Methanothermobacter thermautotrophicusDepositorInsertsDownstream homologous region
thermostable neomycin phosphotransferase gene
Upstream homologous region
UseTagsExpressionMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMX-IG-ATG3 (H262A)
Plasmid#212027PurposeExpresses untagged ATG3 (H262A) in mammalian cellsDepositorInsertATG3 (ATG3 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLINE1ΔORF2-scFvFc
Plasmid#206021PurposeThe ORF2 sequence was removed from pLINE1-scFvFcDepositorInsertmouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF2 sequence
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLINE1ΔORF1-2-scFvFc
Plasmid#206022PurposeThe ORF1 and ORF2 sequences were removed from pLINE1-scFvFcDepositorInsertmouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF1 and ORF2 sequences
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF366
Plasmid#155332PurposeExpression of FLuc under the control of AtAP1 promoterDepositorInsertPAtAP1-FLuc-TSV40
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGA-red-mini
Plasmid#196338PurposeEasy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGA-red-maxi
Plasmid#196337PurposeEasy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGA-blue
Plasmid#196336PurposeEasy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRA1SEGFPTcIfm
Plasmid#193777PurposeCassette 1: Expresses EGFP under the control of PR promoter, Cassette 2: Expresses frame-shifted CI under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selectionDepositorInsertEGFP and frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPR promoter and PLTetO-1Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-DrCavin1b
Plasmid#194292PurposeMultisite gateway entry clone for expression of codon optimised zebrafish cavin1b with fusion tag at the N-terminus. Parton lab clone KXJDepositorInsertcavin1b (cavin1b Zebrafish)
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWPT-mEGFP
Plasmid#190606PurposeObtained by creating a deletion inside the mCherry coding sequence in pWPT-/GCCACC-mEGFP-IRES-mCherry (Addgene #49235)DepositorInsertsmEGFP
mCherry
UseLentiviralTagsExpressionMutationa deletion was created in mCherry CDS impairing i…PromoterEIF1-shortAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-PBD-GFP-U6ac-rac2 guides
Plasmid#168251Purpose"label active Rac under Rac2 neutrophil-specific knockout background"DepositorInsertPBD GFP
UseZebrafish expressionTagsExpressionMutationPromoterLyzCAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
eDrIR1
Plasmid#183998PurposeOptically controlled eDrIR1 expression in mammalian cellsDepositorInserteDrIR1 (INSR Human)
UseTagsIg kappa secretory signal, mCherry, Deinococcus r…ExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQDKN0760
Plasmid#185629PurposeModified tobacco rattle virus RNA2 vector expressing an sgRNA fused to trucated flowering locus T (BsaI-domesticated) which targets phytoene desaturase (PDS) of Nicotiana benthamianaDepositorInsertsgRNA
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0KN0751
Plasmid#185628PurposeModified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using BsaIDepositorInsertlacZ
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0001
Plasmid#185625PurposeMoClo Level 1, position 2 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoterDepositorInsertSpCas9
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-foxd4l1.1-deltaRII-Cterm-myc
Plasmid#185505PurposeExpression of Xenopus laevis foxd4DepositorInsertfoxd4l1.1
UseIn vitro transcriptionTagsmycExpressionMutationRII domain and C-term deletedPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_HLA-cmyc-GRM3 C127A TMD Y616M
Plasmid#140454PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A and Y616M mutantDepositorInsertGRM3 (GRM3 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanine, tyrosine 616 to methioni…PromoterAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only