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Showing: 6401 - 6450 of 18950 results

  1. pYPQAT35

    Plasmid
    #223407
    Purpose
    T-DNA vector for SpCas9-D10A based A-to-G base editing for monocot plants; NGG PAM; SpCas9D10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-ecTadA8e-SpCas9-D10A-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223407-plasmid-map-sequence-id-452579

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  2. pYPQAT36

    Plasmid
    #223408
    Purpose
    T-DNA vector for SpCas9-D10A based A-to-G base editing for monocot plants; NGG PAM; SpCas9D10A-ABE was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-ecTadA8e-SpCas9-D10A-ZmUbi-gRNA scaffold-tRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223408-plasmid-map-sequence-id-454249

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  3. pYPQAT37

    Plasmid
    #223409
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    AtUBQ10-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223409-plasmid-map-sequence-id-453514

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  4. pYPQAT38

    Plasmid
    #223410
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants select.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223410-plasmid-map-sequence-id-453577

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  5. pYPQAT39

    Plasmid
    #223411
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants select.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223411-plasmid-map-sequence-id-454175

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  6. pYPQAT40

    Plasmid
    #223412
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223412-plasmid-map-sequence-id-453531

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  7. pYPQAT41

    Plasmid
    #223413
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants select.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223413-plasmid-map-sequence-id-452335

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  8. pYPQAT42

    Plasmid
    #223414
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-ecTadA8e-SpRY-D10A-ZmUbi-gRNA scaffold-tRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223414-plasmid-map-sequence-id-454172

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  9. pYPQAT43

    Plasmid
    #223415
    Purpose
    T-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for monocot plants; TTTV PAM; LbCas12a-D156R and the crRNA were driven by separate ZmUbi1 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-LbCas12a-D156R-ZmUbi-crRNA scaffold
    Use
    CRISPR; T-dna vector
    Tags
    NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223415-plasmid-map-sequence-id-453575

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  10. pYPQAT06

    Plasmid
    #223378
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9-ZmUbi-gRNA scaffold-tRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223378-plasmid-map-sequence-id-454174

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  12. SRP19 in pLX311

    Plasmid
    #233292
    Purpose
    Expression of WT SRP19
    Depositor
    Sefi Rosenbluh
    Insert
    SRP19 (SRP19 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    E1Fa
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    233292-plasmid-map-sequence-id-466535

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  15. HaloTag-LacI-mHP1a

    Plasmid
    #232637
    Purpose
    lac repressor (LacI) which binds lacO sites; labeled with HaloTag and coupled with effector protein mouse HP1alpha
    Depositor
    Karsten Rippe
    Insert
    HaloTag-LacI-mHP1a (Cbx5 Mouse, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    232637-plasmid-map-sequence-id-463625

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  16. pMVRha

    Plasmid
    #229553
    Purpose
    Broad-host range plasmid (pBBR1), contains an oriT (RP4) for conjugation in kanamycin sensitive non-model bacteria. The GOI can be cloned under the control of a rhamnose-inducible promoter using BsaI.
    Depositor
    Sandy Schmidt
    Article
    Vajente et al ACS Synth Biol. 2024 Oct 31. doi: 10.102
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    229553-plasmid-map-sequence-id-461178

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  22. pHPHS800

    Plasmid
    #228240
    Purpose
    Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination
    Depositor
    Arvind Rasi Subramaniam
    Article
    Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.
    Insert
    Cas9
    Use
    Tags
    Hygromycin phosphotransferase
    Expression
    Mammalian
    Mutation
    Promoter
    none
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228240-plasmid-map-sequence-id-454866

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  31. pHR-IDR4-mCh-Cry2WT-NLS

    Plasmid
    #221920
    Purpose
    Expression of CBP IDR4 fragment fused to optogenetic protein mCh-Cry2WT
    Depositor
    Daniel Bose
    Insert
    CBP IDR4 (aa 662-787) (CREBBP Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    insertion of the IDR4 region (aa 662-787); insert…
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221920-plasmid-map-sequence-id-437714

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  32. pHR-CFID-mCh-Cry2WT-NLS

    Plasmid
    #221923
    Purpose
    Expression of CBP CFID fragment fused to optogenetic protein mCh-Cry2WT
    Depositor
    Daniel Bose
    Insert
    CBP CFID (aa 1892-2441) (CREBBP Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    insertion of the CBP-FUS interaction domain (CFID…
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221923-plasmid-map-sequence-id-437710

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  33. pHR-IDR3-mCh-Cry2WT-NLS

    Plasmid
    #221919
    Purpose
    Expression of CBP IDR3 fragment fused to optogenetic protein mCh-Cry2WT
    Depositor
    Daniel Bose
    Insert
    CBP IDR3 (aa 441-587) (CREBBP Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    insertion of the IDR3 region (aa 441 - 587); inse…
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221919-plasmid-map-sequence-id-437708

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  34. pHR-AL-mCh-Cry2WT-NLS

    Plasmid
    #221924
    Purpose
    Expression of CBP autoregulatory loop (AL) fragment fused to optogenetic protein mCh-Cry2WT
    Depositor
    Daniel Bose
    Insert
    CBP AL (aa 1529-1607) (CREBBP Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    insertion of the autoregulatory loop (AL) (aa 152…
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221924-plasmid-map-sequence-id-437707

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  35. pHR-IDR7-mCh-Cry2WT-NLS

    Plasmid
    #221922
    Purpose
    Expression of CBP IDR7 fragment fused to optogenetic protein mCh-Cry2WT
    Depositor
    Daniel Bose
    Insert
    CBP IDR7 (aa 2109-2434) (CREBBP Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    insertion of the IDR7 region (aa 2109 - 2434); in…
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221922-plasmid-map-sequence-id-437705

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  36. pHR-IDR6-mCh-Cry2WT-NLS

    Plasmid
    #221921
    Purpose
    Expression of CBP IDR6 fragment fused to optogenetic protein mCh-Cry2WT
    Depositor
    Daniel Bose
    Insert
    CBP IDR6 (aa 1856-2090) (CREBBP Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    insertion of the IDR6 region (aa 1856-2090); inse…
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221921-plasmid-map-sequence-id-437701

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  37. pHR-IDR1-mCh-Cry2WT-NLS

    Plasmid
    #221918
    Purpose
    Expression of CBP IDR1 fragment fused to optogenetic protein mCh-Cry2WT
    Depositor
    Daniel Bose
    Insert
    CBP IDR1 (aa 72-175) (CREBBP Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    insertion of the IDR1 region (aa 72 - 175); inser…
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221918-plasmid-map-sequence-id-437702

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  38. pAcGFP-N1_CBPdCFIDKTG

    Plasmid
    #221917
    Purpose
    Transient expression of GFP-tagged CBPdeltaCFIDKTGL in mammalian cells
    Depositor
    Daniel Bose
    Insert
    CBP (CREBBP Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    deletion of the CBP-FUS interaction domain (CFID)…
    Promoter
    Available since
    June 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    221917-plasmid-map-sequence-id-437438

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  39. pAG426-GAL-APP-CTF

    Plasmid
    #181732
    Purpose
    Galactose inducible expression of APP-CTF
    Depositor
    Alejandro Chavez
    Insert
    APP-CTF (APP Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    C-terminal fragment (aa 671-770)
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    181732-plasmid-map-sequence-id-437192

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Showing: 6401 - 6450 of 18950 results